Neisseria elongata subsp. nitroreducens 에 의한 감염성 심내막염 1 예

원선영,정두련,주광로,이미숙,조용균,김은옥,김양수,우준희,배직현,류지소 대한화학요법학회 1998 대한화학요법학회지 Vol.16 No.2

Neisseria elongata subsp. nitroreducens는 Neisseria elongata의 세 가지 아종 중에서 인체에 병원균으로 작용하는 세균으로서 주로 심내막염을 일으키며 드물기는 하지만 그 증례 수가 점차 증 가하고 있으며 국내에서는 아직 보고된 바가 없었다. 저자들은 이전에 심질환의 병력이 없던 36세의 남자에서 치과치료 후 발생한 N. elongata subsp. nitroreducens에 의한 심내막염을 경험하여 문헌고찰과 함께 보고하는 바이다. N. elongata subsp. nitroreducens에 의한 심내막염은 경과 중 판막 파괴를 많이 초래하여 판막치환술이 필요했던 경우가 많았던 것의로 보고되고 있으나 본 증례에서는 4주간의 항균제 치료만으로 합병증 없이 호전되었다. Neisseria elongata subsp. nitroreducens differs from the other subspecies of N. elongata in additional reduction of nitrate without gas formation. Like most Neisseria, species except Neisseria meningitidis and Neisseria gonorrheae, N. elongata subsp. nitroreducens is usually classified in the group of non-pathogenin Nisseria. However, N. elongata subsp. nitroreducens, usual commensal of nasopharynx, can cause serious infections, usually endocarditis. To date, infective endocarditis with N. elongata subsp. nitroreducens has not been reported in Korea. We report a case of 36 year-old Korean male with Neisseria elongata subsp. nitroreducens endocarditis who was treated with antimicrobial agents without complication.

대장의 혼합성 증식성 선종성 폴립 (톱니 선종)에서 Ki-67과 p53 단백의 발현

원선영,홍은경,이중달 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.2

Pathologic characteristics of 23 mixed hyperplastic adenomatous polyps(serrated adenoma) of the large intestine are compared with those of hyperplastic polyps and adenomatous polyps. Expressions of Ki-67 and p53 monoclonal antibodies are studied in serrated adenomas in order to clarify the neoplastic potentiality. Serrated adenomas are characterized by architectural patterns of hyperplastic polyp and cytologic features of adenomatous polyp. Various degrees of dysplasia are present in the crypt epithelium of serrated adenoma. Expression of Ki-67 protein is noted in moderate degree in upper zone of crypts in 12 out of 21 cases(57%) of serrated adenoma, the finding similar to those of adenomatous polyp(44%). Hyperplastic polyps reveal Ki-67 expression confined to the lower zone of their crypts only in a single case, the findings identical to that of normal colonic crypts. p53 monoclonal antibody is reactive for serrated adenomas (2/13), hyperplastic polyps(1/6) and adenomatous polyps (4/13) in mild degree. However, each case of serrated adenoma and adenomatous polyp show p53 protein expression in the upper and middle zones of the crypts. The histopathologic features and expressions of Ki-67 and p53 protein indicated that serrated adenomas have features more similar to adenomatous polyps rather than hyperplastic polyps. The study indicates that serrated adenomas represent in reality neoplastic process.

조건적 운동이 자폐성 아동의 부적절 손대기에 미치는 영향

원선영 단국대학교 사범대학 특수교육과 사례연구회 2001 사례연구집 Vol.7 No.-

도시 복합용도시설에 나타난 ‘지역성’의 특성에 관한 연구

원선영(Won, Sun-Young),이효창(Lee, Hyo-Chang),박정아(Park, Jung-Ah),하미경(Ha, Mi-Kyoung) 한국실내디자인학회 2008 한국실내디자인학회 학술대회논문집 Vol.10 No.1

척수성 근위축증 환자의 유전형과 표현형에 대한 상관관계 연구

원선영(Seon-Young Won),유경화(Kyong-Hwa Ryu),이은하(Eun-Ha Lee),한시훈(Si-Houn Hahn),배기수(Ki-Soo Pai),김성환(Sung-Hwan Kim) 대한소아신경학회 1999 대한소아신경학회지 Vol.7 No.1

배 경 : 척수성근위축증(SMA)은 척수 전각세포나 뇌간핵의 변성 또는 소실로 인해 점진적 인 근력의 약화나 근위축을 초래하는 질환으로 상염색체상에서 열성 유전되는 소아 신경근질환이다. 이 질환은 염색체 5번 장완(5q11.2-13.3)에 존재하는 여러 candidate gene, 즉 SMN, NAIP 및 p44 유전자의 결함으로 발생되는 것으로 본다. Candidate 유전자들의 분자적 특징이 증명되었음에도 불구하고, 유전양상과 임상양상과의 상관관계는 아직 불분명한 상태이다. 유전자의 결손과 같이 유전자 conversion도 중요한 기작으로 부각되고 있으며, 유전양상과 임상양상과의 상관관계에 대한 연구에는 많은 어려움들이 나타나고 있다. 목 적 : 한국인 척수성 근위축증 환자에서 SMN, NAIP 및 p44 유전자의 척수성 근위축증 표현형에 미치는 영향을 관찰하여 유전적인 양상과 표현형 사이의 상관관계를 규명하고, 유전자의 결손뿐만 아니라 유전자의 conversion이 질환 발현에 중요한 기전이 될 수 있음을 규명하고자 하였다. 또한 이 연구에서는 정상 신생아에서의 유전자의 rearrangement의 빈도를 조사하였다. 방 법 : 척수성 근위축증에 영향을 주는 candidate 유전자인 SMN 유전자의 exon 7과 exon 8, NAIP 유전자의 exon 5와 p44 유전자 5'말단의 C15l유전자의 결손을 확인하기 위해 각각의 primer을 이용하여 PCR 증폭을 하였으며, PCR 산물들을 각각 DraⅠ, DdeⅠ, Alu의 제한효소를 처리하여, 결손 여부를 관찰하였다. 또한, p44 유전자의 3'말단의 N1839부위는 SSCP로 분석하였다. 결 과 : 1) 제 1형 환자 8명과 제 2형 환자 2명에 대한 조사에서 제 1형 환자 5명에서 SMN 유전자 뿐만 아니라, NAIP와 p44 유전자의 결손을 관찰하였고 나머지 제 1형의 3명과 제 2형 모두에서는 SMN 유전자만이 결손 되어 있었다. 2) 본 연구 대상아 100명에서 SMN와 NAIP 유전자의 결손 여부를 관찰한 결과 NAIP 유전자는 모두 존재하였으나, centromeric SMN 유전자가 2%정도에서 결손 되어 있음을 확인하였다. 3) SMN, NAIP 및 p44 유전자 모두 결손을 보이는 제 1형 환자 중 telomeric exon 8만이 존재하는 환아 1명에서 telomeric exon 7이 centromeric exon 7로 유전자 conversion이 발생된 것을 확인하였다. 결론 : 척수성 근위축증 중 제 1형에서만 candidate 유전자인 SMN, NAIP 및 p44 유전자의 결손이 발견되어 이 세 가지 유전자 모두의 결손이 임상적으로 심한 양상과 관련이 높은 것으로 추정되나 SMN 유전자만 결손된 경우도 제 1형과 제 2형 모두에서 발견되어 SMN 유전자결손 외에 또 다른 요소가 임상양상에 영향을 주는 것으로 추정된다. 한 명의 제 1형 환자에서 관찰된 centromeric SMN 유전자의 telomeric 위치로의 gene conversion은 SMN 유전자 copy수와 임상양상간의 상관관계가 불분명함을 제시하고 있다. 정상아에서 관찰된 높은 빈도의 유전자 rearrangement는 산전진단시 주의를 필요로 하는 것으로 생각된다. Background : Spinal muscular atrophy(SMA) is the second most common disease with autosomal recessive mode of inheritance in children and characterized by degeneration of anterior horn cells of the spinal cord resulting in weakness and wasting of voluntary muscles. This disease is caused by deletion of many candidate genes including SMN, p44, NAIP on chromosome 5q11.2-13.3. Although molecular characteristics of candidate genes were identified, genotype-phenotype correlation has not been clearly elucidated yet. Nevertheless, gene conversion, previously described as simply as gene deletion, appears to be very important mechanism as a molecular pathogenesis, and even makes more difficult to pursue the correlation. Purpose : This study was aimed to define the correlation between genotype and phenotype of SMA in Korean patients. The significance of SMN gene as well as NAIP gene, p44 gene in the progress of disease process and phenotypic correlation with gene conversion was evaluated. This study was also undertaken to determine the frequency of gene rearrangements in normal population. Method : Eight type Ⅰ SMA patients and two type Ⅱ SMA patients were studied. SMN, NAIP, and p44 gene deletion were analysed by PCR amplification and restriction enzyme digestion with DraⅠ, DdeⅠ and AluⅠ, respectively. p44 gene was also analyzed by SSCP. Gene conversion was defined by centromeric and telomeric SMN gene exon 7 to exon 8 PCR amplification followed by DdeⅠ restiction enzyme digestion. Result : 1) Five of eight type Ⅰ patients showed deletion of SMN, NAIP and p44 gene, while the rest of type Ⅰ and all type Ⅱ patients showed deletion of SMN gene only. 2) We examined SMN and NAIP gene deletion on 100 normal newborns, which showed the deletion of centromeric SMN gene in two newborns, the relative frequency of 2% in gene rearrangement. 3) There was one case of type Ⅰ SMA showing deletion of telomeric SMN exon 7 but not SMN axon 8 suggestive of gene conversion occurred during the recombination as a molecular pathogenesis. Conclusion : The major deletion of SMA candidate genes, SMN, NAIP, and p44 gene appear to be involved in severe phenotype since these three candidate genes deletion were noted only in type 1 cases. However, SMN gene deletion only identified both in type 1 and type 2 explains that SMN gene may plan an major role in the pathogenesis of SMA and also suggests that other factors may be affecting the severity in spinal muscular atrophy. One patient with type Ⅰ which showed the conversion of the centromeric SMN gene to the teleomeric gene strongly supports that SMN gene copy number may not be correlated with the severity in SMA. Our molecular findings suggest that phenotype is not clearly correlated with genotype. Prenatal screening should be carefully undertaken to interpretate because of high frequency of gene rearrangements in normal populations.

제5차 대한간학회 춘계학술대회 초록집 : 포스터 토의 순서 ; 한국인 간질환에서 TT virus의 감염율

원선영 ( Won Seon Yeong ),송병철 ( Song Byeong Cheol ),박무인 ( Park Mu In ),정영화 ( Jeong Yeong Hwa ),이영상 ( Lee Yeong Sang ),서동진 ( Seo Dong Jin ) 대한간학회 1999 Clinical and Molecular Hepatology(대한간학회지) Vol.5 No.1(S)

대학교 캠퍼스 내ㆍ외부 보행환경의 ‘접근성’에 관한 연구

원선영(Won, Sun-Young),김성준(Kim, Sung-Jun),이효창(Lee, Hyo-Chang),김대진(Kim, Dae-Jin),박정아(Park, Jung-Ah),하미경(Ha, Mi-Kyoung) 한국생태환경건축학회 2009 한국생태환경건축학회 논문집 Vol.9 No.1

한국인 간질환에서 TT virus 감염의 유병률

원선영(Sun Young Won),서동진(Dong Jin Suh),송병철(Byung Cheol Song),이한주(Han Chu Lee),정영화(Young Hwa Chung),이영상(Yung Sang Lee) 대한내과학회 2000 대한내과학회지 Vol.59 No.6

N/A Background : TT virus (TTV), an unenveloped single-stranded DNA virus, has been reported in patients with non A-G posttransfusion hepatitis in Japan. Since it has been distributed globally afterward, it was suggested to be a possible causative agent in a proportion of cases with cryptogenic acute and chronic hepatitis. Therefore, we investigated the prevalence and clinical significance of TTV infection in Korean patients with acute and chronic liver diseases. Methods : TTV DNA was determined by polymerase chain reaction with semi-nested primers in sera of 94 patients with liver diseases: 38 with non-ABC liver diseases, 33 with HBV infection and 23 with HCV infection. Results : TTV DNA was detected in 7.9% of non-ABC group, 6.1% of HBV group and 13.0% of HCV group. The positive rates of TTV DNA among three groups were not different statistically. The prevalences of TTV in acute and chronic liver disease were not different. For our study, six out of eight patients with positive TTV DNA had a past history of transfusion. Conclusion : Although TTV infection exists in Korea, it seems unlikely that the virus is responsible for liver diseases of unknown etiology.(Korean J Med 59:612-617, 2000)

아파트 단지 범죄불안감 감소를 위한 환경계획에 관한 연구

원선영(Won Sun-Young),김나연(Kim Na-Yeon),이효창(Lee Hyo-Chang),하미경(Ha Mi-Kyoung) 대한건축학회 2009 대한건축학회논문집 Vol.25 No.2

Modern city penerates into the industrial society and crime which is occurred in the city space is rising up seriously. Above all things, residence crime makes resident feel fear of crime. And that causes harmful affects. Modern city's prevention of crime has its limits. I hope that this study will contribute to the collecting and analysis of the crime phenomenon and the establishment of effective crime prevention strategies in our society. Six apartment complexes are selected. They are located in Gang-nam. A questionnaire survey of 210 residents, the exhaustive analysis and questionnaire of site plans were used to achieve the objective of the study. The results of this study are the followings: 1) The gateway of apartment, like a boundary of outside person's entrance should strengthen cleaning and managing condition, night lighting and security facilities. 2) Parking space should strengthen security facilities and guard condition. 3) Living in the different apartment residents use the Park, playground and pedestrian frequently. So, it should strengthen the security facilities. 4) Elevator should strengthen the security facilities.

Erwinia carotovora subsp. carotovora에 의한 비트 세균성 무름병

박덕환,원선영,황수경,원선영,임춘근 한국식물병리학회 1998 Plant Pathology Journal Vol.14 No.5

Bacterial soft rot caused damage to beets massively grown in Pyungchang, Kangwon province, Korea. The affected roots and stems became cream colored and slimy, and turned black, causing the plants to become wilt and die. The casual organism isolated from the infected plants was identified as Erinia carotovora subsp. carotovora based on physiological and biochemical characteristic, and the results of the Biolog program (Biolog Inc., U.S.A.). E. carotovora subsp. carotovora is the first discribed bacterium which causes bacterial soft rot on beet in Korea.