지속가능한 해삼 양식장 조성을 위한 생태적합 서식처 모형 개발

오윤화,강민선,위진희,이인태,Oh, Yoon Wha,Kang, Min-Seon,Wi, Jin Hee,Lee, In Tae 응용생태공학회 2015 Ecology and resilient infrastructure Vol.2 No.1

본 연구는 우리나라 생태환경에 맞는 해삼(Holothuroidea, de Blainville, 1834)의 생태적합서식 모형을 개발하고자 해삼의 주요 서식지인 백령도, 진도, 울진 해역의 수치조류도, 해황 및 저서환경을 조사하였다. 조사결과, 수온은 $12{\sim}18^{\circ}C$정도로 모두 냉수대 지역이며 표층과 저층 사이의 해수 순환이 활발하였다. 백령도와 진도의 수치조류도 분석 결과, 해수 흐름이 $100{\sim}120cm\;s^{-1}$ 정도로 강한 유속장이 형성되는 특징을 보였다. 또한 세 지역 모두 저서에는 은신처 역할을 하는 암반과 전석 등이 고루 분포하며 다양한 해조류 군락 및 저서생물이 해삼과 함께 서식하였다. 이와 같이 해삼 서식처의 공통적인 특징을 바탕으로 사니질 저질, 다양한 해조류 군락, 저서동물 그리고 암반과 전석 등이 고루 분포하는 형태의 해삼생태적합서식처 모형을 개발하였다. 본 모형의 치삼 서식지는 조간대 지역으로 암초 및 해조류가 분포하며, 퇴적물의 구성은 펄질로 나타내었으며 성삼 서식지는 사질과 니질이 만나는 곳에서 가장 많은 해삼 분포와 수심이 깊어질수록 큰 개체를 나타내었다. 또한, 조간대보다 큰 전석과 해조류가 다수 분포하는 형태로 나타내었다. We investigated the tidal current, hydrographic data, and benthic environment of major sea cucumber (Holothuroidea, de Blainville, 1834) habitats in Baengnyeongdo, Jindo and Uljin to understand the optimal environmental or ecological habitat for sea cucumbers. The three study areas were characterized by a cold-water mass of temperatures ranging $12{\sim}18^{\circ}C$, with an active circulation between the surface and deep waters. According to an analysis of the tidal current map, a strong flow velocity of $100{\sim}120cm\;s^{-1}$ appeared in Baengnyeongdo and Jindo. The three sea cucumber habitats showed the common characters of a bottom sediment composed of sand-silt, a diverse seaweed colony and benthic organisms, and boulders and rocks which provide a hideout for the organisms. We aimed to draw the optimal habitat condition for sea cucumbers in Korea, and the result showed that the low water temperature, rapid water flow, active vertical mixing between surface and deep waters, bottom composed by sand-silt, large rocks, and diverse seaweed colony and benthic organism were important factors. The optimal habitat for Juvenile sea cucumbers was the intertidal areas characterized by a muddy bottom, reef, and seaweed. The optimal habitat for adult sea cucumbers was characterized by a place where sand and mud are mixed, and the body size of the sea cucumber was proportional to water depth, and the relatively large boulders and rocks compared to the intertidal area.

Sympathetic regulation of aquaporin water channels in rat kidney

오윤화 ( O Yun Hwa ),김수완 ( Kim Su Wan ),유광재 ( Yu Gwang Jae ),임동윤 ( Im Dong Yun ),김남호 ( Kim Nam Ho ),최기철 ( Choe Gi Cheol ),이종은 ( Lee Jong Eun ) 대한신장학회 2002 춘계학술대회 초록집 Vol.21 No.1

허혈/재관류 손상을 유발한 흰쥐 신장에서 산화질소합성 억제가 신장의 Aquaporin 수분통로 발현에 미치는 영향

마성권,오윤화,박춘순,이연경,김수완,김남호,최기철,이종은 대한신장학회 2006 Kidney Research and Clinical Practice Vol.25 No.1

초등 과학 교수학습 활동 유형에 대한 초등학생들의 감성 상태

임채성,오윤화 韓國生物敎育學會 2004 생물교육 Vol.32 No.4

To investigate the relationship between emotional states of elementary school students and learning science, the emotional aspects of activities involved in learning science were analyzed. 77 sixth graders' perceptions on various activities performed in science instruction were discerned through a questionnaire. The emotional states perceived by the students were divided into two categories of the positive and negative ones. The students' responses were analyzed in terms of the content, the teaching and learning methods, and the reasons why they perceived those aspects as positive or negative. Major results are as follows. First, for the reasons why they perceived the science learning experience as positive, the factors associated with Processes of Learning appeared as most prominent. whereas the factors related to Learning Materials were most prominently perceived as negative. Second, for the characteristic ways of learning science in both emotional states, it was shown that Experimentation was a typical method which provided students the joyfulness of physical activities and encouraged curiosity. The learning method which most frequently perceived as negative was Observation. Finally, the students suggested Experimentation as most preferred activity. while activity of Constructing Report as most not-preferred one. In general, the learning activities to be performed alone, and felt as static, routine, or meaningless to students were all contributed to the negative emotional states of the learners. These results have fundamental implications for the development of emotionally desirable science learning environments and for the design of further research on learning.

아동이 지각한 감성 상태와 과학 학습과의 관계

임채성,오윤화 부산교육대학교 과학교육연구소 1999 科學敎育硏究 Vol.24 No.-

To investigate the relationship between emotional states of children and science learning, we analyzed the memories of school science with regard to the emotional states of them. Seventy seven sixth graders' memories on the science instruction which they have received so far were discerned through a questionaire and interviews. The emotional states of the children were divided into two, that is, the positive and negative states. The children's responses were categorized into the amount of memory, content and learning methods, and the reasons why them perceived as positive or negative. The results as follows. First, in the quantitative aspect, it was found that the science learning experiences perceived as positive sere much more effective than those of perceived as negative. In the qualitative aspects, more science concepts were formed from the learning at the positive emotional states than those at the negative states, furthermore, it was found that in associating their learning with the processes they performed, the former was more effective. Second, for the reasons why they perceived the science learning experience as positive, the factors associated with processes of learning were most typical, and in those of perceived as negative the factors related to the learning materials were most prominent. For both of positive and negative emotional states, personal factors showed to get a second priority. Finally, for the characteristic ways of science learning in both of emotional states, Experimentation was typical method which provided children the joyfulness of physical activities and new curiosity, also it was shown to be most preferred activity by the children. While, the learning method which most probably perceived as negative emotional states by children was Observation. The reasons for the method contained the learning materials they dislike and the limitation of time. In general, the learning activities performed alone, static activities, and activities felt as routine or meaningless to children were all significantly contributed to the negative emotional states of learners. These findings provide a basis for understanding the roles of emotion in learning science. The results also highlight the need for teachers to provide experiences and to create environments which encourage the positive emotional states of learners when teach science subjects.

초등학교 학생이 지각한 감성 상태와 과학 학습 경험에 대한 기억의 관계

임채성,오윤화 韓國生物敎育學會 2004 생물교육 Vol.32 No.2

To investigate the relationship between emotional states of children and science learning, we analyzed the memories of school science experiences with regard to the children's emotional states. Seventy seven sixth graders' memories on the science instruction which they have received were discerned through a questionaire and interviews. The emotional states of the children were divided into two, that is, the positive and negative states. The children's responses were analyzed with respect to the amount of memory on science learning experiences and the scientific and nonscientific concepts. The results are as follows. First, in the quantitative aspect, it was found that the amount of memory on science learning experiences perceived by the children as positive emotion were higher than those of perceived as negative one. Second. when the children experienced science learning activities in the positive emotional states, their memory on them were much more interrelated across contents, whereas in the negative emotional states their memory were fragmented. Finally, in the qualitative aspect, more scientific concepts were formed from the learning at the positive emotional states than those at the negative states. These findings provide a basis for understanding the roles of emotion in tehaching and learning science. The results also suggest the need for teachers to provide experiences and to create environments which encourage the positive emotional states of learners when teach science subjects.

Upregulation of Heat Shock Proteins in the Kidney in Hypertension

이건,오윤화,이종은 대한약리학회 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.3

The present study was undertaken to determine the regulation of heat shock proteins (HSP) in the kidney in hypertension. Two-kidney, one clip (2K1C) or deoxycorticosterone acetate (DOCA)-salt hypertension was induced in male Sprague-Dawley rats. At weeks 1 and 4 after inducing the hypertension, the expression of HSP70, HSP32 and HSP25 was determined in the kidney by Western blot analysis. In 2K1C hypertension, the expression of HSP70, HSP32 and HSP25 was increased in the clipped kidney at both weeks 1 and 4. However, in the contralateral kidney, their expression was not significantly altered at week 1, but increased at week 4. In DOCA-salt hypertension, the expression of HSP remained unaltered in the remnant kidney at week 1, but significantly increased at week 4. These results indicate that HSP are differentially regulated in the kidney according to the duration and the model of hypertension.

Upregulation of Renal Renin-Angiotensin System in Rats with Adriamycin-Induced Nephrosis

김수완,JongUnLee,한상웅,류준호,오윤화,김남호,최기철,김호중 대한약리학회 2002 The Korean Journal of Physiology & Pharmacology Vol.6 No.2

신혈관성 고혈압 흰쥐 신장에서 Na, K-ATPase 단백 발현 감소

마성권 ( Seong Kwon Ma ),오윤화 ( Yoon Wha Oh ),김인진 ( In Jin Kim ),배은희 ( Eun Hui Bae ),이종은 ( Jong Un Lee ),김수완 ( Soo Wan Kim ) 대한내과학회 2010 대한내과학회지 Vol.78 No.4

목적: 본 연구는 two kidney, one clip (2K1C) 신혈관성 고혈압을 유발한 흰쥐에서 신장의 Na,K-ATPase, 레닌-안지오텐신-알도스테론계(renin-angiotensin-aldosterone system, RAAS) 및 심방나트륨이뇨호르몬(atrial natriuretic peptide, ANP)계가 고혈압 및 신장 손상의 병태생리에 관련되어 있는지를 조사하고자 하였다. 방법: 실험재료는 Sprague-Dawley 숫쥐를 사용하였다. 2K1C 고혈압을 유발하기 위하여 ketamine (50 mg/kg, i.p.) 마취 후 왼쪽 신동맥에 0.25mm 내경의 silver clip을 끼웠다. 4주일 후에 tail-cuff 방법을 이용하여 수축기 혈압을 측정하였으며, 마취하지 않은 상태에서 단두하여 신장을 적출하였다. 신장 조직에서 Na,K-ATPase α1 subunit 단백 발현을 Western blot 분석법에 의하여 조사하였다. 또한, renin, angiotensin-converting enzyme (ACE), aldosterone synthase (CYP11B2), ineralocorticoid receptor (MR) 및 ANP계의 mRNA 발현을 real-time polymerase chain reaction으로 조사하였다. 결과: 실험군에서 수축기 혈압이 유의하게 증가하였으며, 혈장 레닌 활성도 및 혈청 알도스테론 농도가 증가하였다. 또한, 소변량 및 나트륨 분획 배설이 유의하게 증가하였다. Na,K-ATPase α1 subunit의 단백 발현은 클립 신장에서 대조군에 비하여 유의하게 감소하였고, 클립을 끼우지 않은 실험군의 반대쪽 신장의 단백 발현이 클립 신장에 비하여 증가하였다. 신장 조직의 renin, ACE1, CYP11B2 및 MR의 mRNA 발현은 실험군의 클립 신장에서 대조군에 비하여 증가하였고, 클립을 끼우지 않은 반대쪽 신장에서는 클립 신장에 비하여 유의하게 감소하였다. ACE2 mRNA 발현은 대조군, 실험군의 클립 신장 및 반대쪽 신장 모두에서 유의한 차이가 없었다. 또한, 클립 신장 및 반대쪽 신장의 ANP mRNA 발현이 대조군에 비하여 유의하게 증가하였다. 결론: 신혈관성 고혈압 유발 흰쥐에서 전신 및 신장의 국소 RAAS의 활성 증가가 고혈압 및 세뇨관 손상에 기여할 것으로 사료되며, 신장 ANP계의 활성증가는 고혈압에 대한 보상기전으로 작용할 것으로 보인다. 또한, 세뇨관 손상과 관련된 클립 신장의 Na,K-ATPase 발현 감소가 다뇨 및 나트륨 재흡수 장애에 관여할 것으로 생각된다. Background/Aims: This study investigated the role of Na,K-ATPase, the local renin-angiotensin-aldosterone system (RAAS), and atrial natriuretic peptide (ANP) system in the pathogenesis of renal tubular dysfunction and hypertension in rats with two-kidney, one-clip (2K1C) hypertension. Methods: Adult male Sprague-Dawley rats were made 2K1C hypertensive for 4 weeks. The renal expression of Na,K-ATPase was determined by immunoblotting. The mRNA expression of renin, angiotensin-converting enzyme (ACE), aldosterone synthase (CYP11B2), mineralocorticoid receptor (MR), and the ANP system were determined in the kidney using real-time polymerase chain reaction. Results: The blood pressure was increased in the 2K1C rats, compared with controls. The plasma renin activity and serum aldosterone concentrations were increased, as were the urine output and fractional excretion of sodium. The expression of Na,K-ATPase protein was decreased in the clipped kidney, as compared with the control kidney, while it remained unchanged in the contralateral kidney. The mRNA expression of renin, ACE1, CYP11B2, and MR was increased in the clipped kidney, but unchanged in the non-clipped kidney. The mRNA expression of ACE2 did not differ between the groups. The expression of ANP mRNA was increased in both clipped and non-clipped kidneys, as compared with control kidneys. Conclusions: The enhanced activity of the local RAAS may result in to ischemic tubular injury and the development of hypertension in 2K1C rats. The downregulation of Na,K-ATPase associated with tubular injury in the clipped kidney may account for the impaired tubular sodium reabsorption in 2K1C hypertension. (Korean J Med 78:477-484, 2010)

Influence of Nitric Oxide Synthesis Inhibition on Endothelin-1 and Its Receptor Expression in Rat Kidney

Lee, Jong Un,Kim, Soo Wan,Kim, Sun Mi,Oh, Yoon Wha,Li, Ying Shun,Kim, Nam Ho,Choi, Ki Chul 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

배 경 : 본 연구는 산화질소 합성억제 흰쥐 모델에서신장 endothelin(ET)계의 변화를 알아보고자 하였다. 방 법 : 수컷 Sprague-Dawley 흰쥐를 이용하여 실험하였다. 실험군은 내인성 산화질소 합성을 억제하고자 NG-nitro-L-arginine methyl ester(L-NAME, 100mg/L)을 4주간 음용수로 공급하였다. 대조군은 약물이 포함되지 않은 수돗물을 공급하였다. 신장에서 ET-1,ETA 및 ETB 수용체 mRNA 발현 변화는 역전사-중합효소 연쇄반응을 이용하였다. 혈장 및 신장에서 산화질소 대사물 함량을 측정하였다. 결 과 : 산화질소 합성억제제인 L-NAME 투여군에서 유의한 혈압의 상승을 보였으며, 혈장 및 신장에서 산화질소 대사물은 감소하였다. ET-1 mRNA 발현은 신피질에서 증가하였으나, 신수질에서는 유의한 변화를 보이지 않았다. 신장에서 ETA 및 ETB 수용체 발현 또한 유의한 차이를 보이지 않았다. L-NAME(200 μg/kg per min, iv)를 60분간 주사한 흰쥐에서 유의한 혈압의 증가와 함께 혈장 ET-1 농도가 증가하였다. 또한, 이때 신피질 ET-1 mRNA 발현은 증가하였으나, ETA 및 ETB 수용체 발현 또한 유의한 차이를 보이지 않았다. 결 론 : 이상의 결과는 산화질소 합성 억제에 의한 고혈압의 발생에 ET계의 활성증가가 일부 관여함을 시사한다. Background : The present study was aimed to evaluate the influence of nitric oxide( NO) synthesis inhibition on endothelin(ET) expression in rat kidney. Methods : Male Sprague-Dawley rats were treated with NG-nitro-L-arginine methyl ester(L-NAME, 100 mg/L drinking water) for 4 weeks to inhibit the endogenous synthesis of NO. The tissue expression of ET-1, ETA receptor, and ETB receptor mRNA in the kidney was determined by reverse transcription-polymerase chain reaction. Results : Tissue levels of NO metabolites were significantly decreased in the plasma and the kidney, along with the increased blood pressure. The expression of ET-1 mRNA was increased in the cortex, but not in the medulla. The expression of ETA and ETB receptor mRNA was not significantly altered either in the cortex or in the medulla. The plasma level of ET-1 peptide was significantly increased, along with the increased blood pressure, when L-NAME(200 μg/kg per min, iv) was administered in an acute preparation of animals. Accordingly, the expression of ET-1 mRNA was increased in the cortex, whereas that of ETA and ETB receptor mRNA was not altered. Conclusion : These results suggest that enhanced activity of ET system induced by NO synthesis inhibition may be associated with hypertension although direct association between two factors is not confirmed. (Korean J Nephrol 2002;21(2):205-212)