눈알고둥, Lunella coronata coreensis의 생식주기

오성립,나오수,이영돈,김형배 제주대학교 해양과환경연구소 2000 해양과환경연구소 연구논문집 Vol.24 No.-

눈알고둥, Lunella coronata coreensis의 생식주기

오성립,나오수,이영돈,김형배 제주대학교 해양연구소 2000 해양자원연구소연구보고 Vol.24 No.-

눈알고둥,Lunella coronata coreensisd의 생식주기

오성립,나오수,이영돈,김형배 제주대학교 해양연구소 2000 해양과환경연구소 연구논문집 Vol.24 No.-

We investigated reproductive cycle of the turban shell. Lunella coronata coreensis with the histological preparation of gonads and the gonadosomatic index (GSI). The turban shells were collected at the intertidal zone of Hamdeok in Jeju-Do from December. 1995 to October. 1996. The gonad was located on the surface of the liver below the stomachal caecum Posterior spiral meat part of the shell. GSI value began to increase from May as water temperature increased and reached its maximum value in August both male and female which were 56.94 and 61.88. respectively. GSI started to decrease from September thereafter. maintaining relatively low value from January to March. The reproductive cycle of L. coronata coreensis could be grouped into five successive developmental stages : multiplicative stage (female: February to April. male. April to May). growing stage (female: April to June, male: June). mature stage (female: June to August, male: July to August). ripe and spawning stave (August to September). And degenerative and recovery stage (October to March). The spawning of the turban shell occurred from August to September and the main spawning period appeared in September. The turban shell. L. coronata coreensis appeared to be gonochoristic species. Sex ratio of female to male was 1.6 : 1.0 (P > 0.05).

Ovarian Differentiation of the Scorpion Fish, Sebastiscus marmoratus

오성립,허성표,임봉수,이치훈,이영돈 한국발생생물학회 2006 발생과 생식 Vol.10 No.3

이 연구는 태생경골어인 쏨뱅이의 번식생태학적 기초자료를 제공하고자 체내 자어와 출산 후의 시원생식세포의 출현, 원시 생식소와 난소 분화과정을 조직학적으로 조사하였다. 약 의 시원생식 세포는 체내 자어의 초기 소화관과 중심신관 사이 복막에 위치하였다. 출산 후 31일째부터 생식원세포가 생식원기에 도달하였으며, 체세포들이 분열 증식하여 원시생식소를 형성하였고, 출산 후 49일째에 난소강이 형성되기 시작하였으며, 출산 후 60일째에 생식원기의 양쪽 끝에서부터 The process in the formation of primordial germ cells, primitive and early gonadogenesis were investigated by histological examination for the reproductive physiological study in the scorpion fish, Sebastiscus marmoratus. The primordial germ cells about in diameter were observed in the fibrous mesenchymal tissue located between gut and mesonephric duct of the larvae within the maternal body. As the fibrous epithelium extends to the direction of peritoneum, the primordial germ cells moved. From 31 days post parturition, the fibrous epithelium gets thicker, and the primitive gonad starts to form. From 49 days post parturition, as the primitive gonad of the primitive gonad extend, it starts to form cavity, and about 60 days post parturition, the formation of the ovarian cavity is completed. From 79 days post parturition, germ cells proliferated along the inner edge of the ovarian cavity.

Evaluation of Fertilizing Ability using Frozen Thawed Sperm in the Longtooth Grouper, Epinephelus bruneus

오성립,이치훈,Hyeong-Cheol Kang,송영보,김형배,이영돈 한국발생생물학회 2013 발생과 생식 Vol.17 No.4

This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at different DMSO concentration of 5.0%, 7.5%, 10.0% respectively, FR were (DMSO 5.0%: 99.5±0.8%, DMSO 7.5%: 99.5±0.7%, and DMSO 10.0%: 99.6±0.6%). The results are not significantly different from the control fresh sperm (100%). HR also (DMSO 5.0%: 96.2±2.3%, DMSO 7.5%: 95.3±3.6%, 10.0%: 96.6±1.8%) were not significantly different in each group. The normal individual rate after hatching using with control fresh sperm (98.4%±0.5) and DMSO concentration level of 5.0% (97.8±0.1%) were not significantly different. However, with 7.5% (97.2±0.6%) and 10.0% DMSO concentrations (95.9±0.2%) are lower than the normal individual rate after hatching observed in the control and 5.0% DMSO. Performing artificial fertilization using frozen-thawed sperm at different frozen period (2 days, 2 years, and 3 years), 10% DMSO FR and HR of 3 years (FR; 66.8±1.8%, HR: 82.0±12.9%) and 2 years (FR; 78.5±14.8%, HR: 79.3±0.6%) cryopreserved sperm were lower than control (FR; 100%, HR: 91.1±3.6%) and 2 days cryopreserved sperm (FR; 99.6±0.6%, HR: 96.6±1.8%). These results suggest suitable DMSO concentration ranges of cryopreservation sperm for E. bruneus is 5 to 10% and with 2 to 3 years cryopreservation period, cryopreservation sperm can be useful for seed production.

고전압 정전기장 냉장시스템에 저장한 넙치(Paralichthys olivaceus) 필렛의 이화학적 특성 변화

남종웅,오성립,고형범,원승환,정민정,한아람,김병목,전준영 한국식품저장유통학회 2022 한국식품저장유통학회지 Vol.29 No.6

The effects of a high-voltage electrostatic field-refrigeration (HVEsF) system on the freshness of olive flounder fillets were investigated by evaluating the changes in the physicochemical properties of the fillets for 7 days. After the olive flounders had been euthanized, the fillets were obtained immediately and stored under four different conditions: -1℃ as a control (Con); -1℃ HVEsF system set to 3,500 V (H35); -1℃ HVEsF system set to 7,000 V (H70); and 4℃ (R). During the whole storage period, slight decreases and increases in pH were repeatedly performed in all the groups, and bacterial growth was inhibited at –1℃. The formation of volatile base nitrogen, trimethylamine, and hypoxanthine was significantly lower in H70 than in Con for 7 days (p<0.05). The cutting strength of H70 was maintained until day 3, while that of the other groups decreased drastically as the storage period increased. Overall, a -1℃ HVEsF system set to 7,000 V maintained the freshness of the fillets in the initial stages of storage.

손바닥선인장 열매 발효액 첨가사료가 참돔의 성장에 미치는 영향

스이치 사토,고경민,오성립 한국양식학회 2007 韓國養殖學會誌 Vol.20 No.1

RNA-Seq transcriptome 분석을 통한 갈치 광수용체 유전자 탐색 및 mRNA 조직발현

현지연,김문관,임봉수,변준환,문지성,강형철,허성표,오성립 한국해양과학기술원 2017 Ocean and Polar Research Vol.39 No.2

The opsin family of light sensitive proteins family makes up are the universal photoreceptor molecules of all visual systems in the vertebrates including teleosts. They can change their conformation from a resting state to a signaling state upon light absorption, which activates the G-protein coupled receptor, thereby resulting in a signaling cascade that produces physiological responses. However, this species is poorly characterized at molecular level due to little sequence information available in public databases. We have investigated the opsin family of nocturnal cutlass fish using the whole transcriptome sequencing method. The opsin genes were cloned and its expression in the tissues and organs were examined by qPCR. We cloned 6 opsin genes (RRH, Opn4, Rh1, Rh2, VA-opsin, and Opn3) in retina and brain tissue. It contained the seven presumed transmembrane domains that are characteristic of the Gprotein- coupled receptor family. However, short wavelength sensitive pigment (SWS) and long wavelength sensitive pigment (LWS) were not detected in this study. The mRNA expression of the 6 photoreceptor genes were detected in retina and peripheral tissue. Our studies will lead to further investigation of the photic entrainment mechanism at molecular and cellular levels in cutlass fish and can be used in comparative studies of other fishes.

Bisphenol A 가 송사리 , Oryzias latipes 의 수정란 부화와 어미의 산란에 미치는 영향

나오수(Oh Soo Na),백혜자(Hea Ja Baek),이영돈(Young Don Lee),오성립(Seong Rip Oh),김형배(Hyung Bae Kim) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.5

내분비장애물질로 알려진 BPA가 송사리 수정란의 부화와 어미의 산란량에 미치는 영향을 번식생물학적으로 조사하였다. 송사리 수정란의 부화 소요시간은 대조구 I과 II에서 9∼12일, BPA 50㎍/ℓ 처리구에서 26∼35일, BPA 100㎍/ℓ처리구에서 26∼27일 이었으나, 200㎍/ℓ 처리구에서는 부화하지 않았다. 부화율은 대조구 I과 II에서 각각 89.8, 84.3%인데 비해, BPA 50과 100㎍/ℓ 처리구에서 각각 23.3, 22.2%로 현저하게 낮았다. 송사리 어미가 산란한 알의 수는 대조구 I과 II 그리고 BPA 50㎍/ℓ body weight 처리구에서 각각 52.5, 4.70, 48.0개인데 비해, BPA 100과, 200㎍/ℓ body weight 처리구에서 각각 21.5, 18.5개로 상대적으로 산란량이 적었다. 산란한 모든 알 중에서 비정상란의 비율은 대조구 I과 II에서 각각 11.8, 16.2% 이었고, BPA 50, 100 그리고 200㎍/ℓbody weight 처리구에서 각각 38.8, 74.1%의 비율을 보여 BPA 처리 농도가 높을수록 비정상란이 많았다. 그리고 정상란의 부화율은 대조구에서 평균 78% 이상인데 비해, BPA 처리구에서 평균 50% 이하로 대조구에 비해 상대적으로 낮았다. 이들 결과들에서 BPA 처리구는 대조구에 비해 송사리의 수정란 부화와 송사리 어미의 산란량과 난질 그리고 수정에 저해를 주는 것으로 보인다. The effects of bisphenol A (BPA), known as one of the endocrine disrupting chemicals (EDCs), on the hatching of fertilized eggs (FE) and the spawning of adult fish in songsari, Oryzias latipes were investigated. In condition of the experimental groups, incubated water temperature was 25.0±5℃ and photoperiod was 16 h (light) : 8 h (dark). FE were exposed to aqueous solutions of BPA at nominal concentration of 50, 100 and 200㎍/ℓ. The time required in hatching of FE was long in the BPA 50 and 100㎍/ℓ treatment groups when compared to the controls, and in the BPA 200㎍/ℓ treatment group, FE failed to hatch. And also hatching rate was decreased in the BPA treatment groups in comparison to the controls. Adult fishes were reared to oral administration via a diet of 50, 100 and 200㎍/ℓ body weight BPA concentration for 3 weeks. Number of total eggs spawned in the adult fish were fewer in the BPA 100 and 200 treatment groups than in the controls and BPA 50 treatment group. Frequency of abnormal eggs in the total eggs spawned was 11.8 and 16.2% in the control and acetone carrier control lower than 36.8, 46.8 and 74.1% in the BPA 50, 100 and 200 treatment groups, respectively. And hatching rate of FE decreased in the BPA treatment groups in comparison to the controls. In these results, inhibition of the hatching of FE and the quantity and quality of spawned eggs in adult fish were observed from BPA treatment groups when compared to the controls.