Morphological differences according to uterine tissue remodeling during estrus between sika deer and water deer

오민기,박용수,김상환 사단법인 한국동물생명공학회 2023 Journal of Animal Reproduction and Biotechnology Vol.38 No.4

Background: Water deer and sika deer, which breed in the wild environment, are known to have similar reproductive physiology mechanisms. Therefore, this study aimed to analyze the differences in uterine development between water deer and sika deer during estrus. Methods: MMPs and uterine development-related factors were analyzed and morphological differences were compared in the uterus of sika deer captured near Russia near Korea and water deer captured in the wild in Korea. Results: In terms of morphological differences in the uterus, the glands that form villus within the endometrium of the water deer were newly developed, and the formation of small glands was high, but the villus and glands of the sika deer were expanded, and the stroma zone in the myometrium was higher than that of the water deer. Development has increased. Additionally, the expression of PAPP-A and VEGF factors was increased in the endometrium of water deer than in sika deer, but the actions of MMPs were increased in sika deer. Conclusions: As a result of this study, there is a significant difference in the development of glands in the endometrium of water deer and sika deer during estrus, and it is believed that there is a significant difference in the development of the uterus due to the physiological effects of estrus between water deer and sika deer. Additionally, it is believed that there will be differences in the timing at which pregnancy can be decided.

Histological Study of Oculocutaneous Albinism in the Korean Bitterling, Acheilognathus signifer (Osteichthyes; Cyprinidae)

오민기,박종영,유민정,강언종,양상근,김응오,조용철,박인석,김치홍,Ishinabe Toshihiro 한국어류학회 2008 韓國魚類學會誌 Vol.20 No.3

The Korean bitterling, Acheilognathus signifer (Osteichthyes Cyprinidae), is an endemic and endangered species in Korea. During developmental stages, a small number of oculocutaneous albinism (with colorless body and eyeballs) were observed in the species. In order to investigate histological differences between normal and albinic bitterling, the dorsal skin and choroid-retina of the eyes were taken. The skin and eyes of normal and albino bitterling were similar in structure except for the presence or density of pigment cells. In normal bitterling, the epidermal melanocytes and dermal melanophores were abundantly developed in both the skin and epidermis of the eyes. But in the albino, the dorsal skin had few melanins, and the pigment cells over the choroid-retina pigment epithelium and iris of the eye were very small.

한국산 피라미속(Zacco) 어류의 미토콘드리아 cytochrome b gene 분석을 통한 분자계통

오민기,김익수,박종영 한국어류학회 2010 韓國魚類學會誌 Vol.21 No.4

A molecular phylogenetic relationship inferred from mitochondrial cytochrome b gene sequence was developed based on analysis of Zacco species distributed in Korea as well as China,Japan and Taiwan. A maximum parsimony (MP) tree showed that Korean Z. temminckii and Z. koreanus formed a monophyletic clade, but the populations of Z. temminckii and Z. koreanus in the ‘South Korean Subdistrict’ region had genetic similarity with Japanese Z. temminckii. Korean Z. platypus had a closer relationship with Japanese members of the clade than with Chinese Z. platypus,which was more closely related to Opsariichthys uncirostris amurensis. The analysis of neighbor joining (NJ) tree may support a hypothesis that the clade of Z. platypus had genetically diverged from the common ancestor of Zacco species comprising Z. koreanus, Z. temminckii, Z. sieboldii and other species; thereafter a cladogenesis of Z. koreanus and Z. temmminckii had occurred from the ancestor of Z. sieboldii. Moreover, the Chinese Z. platypus had diverged far from the Korean Z. platypus and formed a phylogenetic relationship with O. uncirostris amurensis. Therefore, a more detailed study of the taxonomy and systematics of Zacco species in regard to their zoogeographical distributions is needed. 한국, 중국, 일본, 대만 등 동아시아에 분포하는 피라미속(Zacco) 어류에 대한 mitochondrial cytochrome b gene의 염기서열 분석을 통해 분자계통학적 유연관계를 조사하였다. MP tree 분석 결과, 한국산 참갈겨니와 갈겨니 집단은 모두단계통군을 형성하고 있었으나 생물분포구계의 남한아 지역에 분포하는 참갈겨니와 갈겨니 집단은 일본산 갈겨니와 유전적으로 유사하게 분석되었다. 또한 한국산 피라미 집단은일본산 피라미와 유전적 친화성을 보였으나, 중국산 피라미는 끄리와 분자계통학적 유연관계를 형성하였다. 참갈겨니의 한강집단은 동해 중북부에 서식하는 집단과 유전적으로유사하였고, 갈겨니의 동진강과 영산강 집단은 유전적 동일집단, 동진강과 섬진강 집단은 유전적 유사집단으로 여겨졌다. NJ tree 분석을 통해 피라미 집단은 참갈겨니와 갈겨니및 Z. sieboldii 집단과는 오래전에 공동조상으로부터 분화되었고, Z. sieboldii의 조상으로부터 참갈겨니와 갈겨니가 분기진화한 것으로 추정되었다. 또한 중국에 서식하는 피라미는 한국산 피라미 집단과 상당한 유전적 차이를 보였을 뿐만 아니라 끄리속 어류와 분자계통수를 형성하고 있어 추후 동물지리학적 분포 및 피라미속 어류에 대한 계통분류학적 논의가 요구된다.

베링해 해빙 상태와 척치해 해빙 변화 간의 연관성 분석: 정보 엔트로피 접근

오민기,김현철 대한원격탐사학회 2018 大韓遠隔探査學會誌 Vol.34 No.6

본 연구에서는 위성영상 기반의 북극의 해빙 농도 시계열 데이터를 이용하여 베링해의 해빙 상태가 척치해 해빙 농도 변화의 전조로서 작용할 수 있는지를 실험하였다. 해빙 농도 자료는 1982년부터 2017년의 36년간의 월평균 시계열 데이터로 이뤄져 있으며, 베링해의 해빙 농도와 척치해 해빙 농도 사이의 관계성을 전송 엔트로피 측정을 통해 분석하였다. 전송 엔트로피는 두 개의 확률변수 또는 신호 간의 비선형적 연관성을 파악하게 해주는 동시에 변수 사이의 시간 간격 조절을 통해 인과관계를 추정할 수 있는 측정이다. 해빙 농도를 대상으로 한 측정 결과, 베링해의 과거 3, 5, 6개월 전의 해빙 농도값이 척치해 해빙의 변화에 관련되어 있음을 알수 있었다. 특히, 베링해의 해빙 농도값이 극소를 나타냈을 때, 5개월 후의 척치해의 해빙 농도는 감소될 확률이 약 70%로 나타났다. 이는 태평양에서 베링해협을 통해 북극해로 유입되는 해류가 베링해의 해빙 농도를 감소시킨 후 해협을 통해 척치해로 이동하여 해빙을 녹이는 과정에 비롯한 것으로 사료된다. 향후 위성데이터에 정보 이론으로 접근하는 이 연구를 더 발전시켜 어떤 시점과 시간적 스케일로 특이 패턴이 발생하는지 조사하고 그 기간에 관련된 해양-대기의 패턴 또는 사건들을 분석하여, 떨어진 두 지역의 해빙 농도 상태에 내재된 연관성에 대한 심층적 이해가 가능할 것이다. We examined if a state of sea-ice in Bering Sea acts as a prelude of variation in that of Chukchi Sea by using satellites-based Arctic sea-ice concentration time series. Datasets consist of monthly values of sea-ice concentration during 36 years (1982-2017). Time series analysis armed with Transfer entropy is performed to describe how sea-ice data in Chukchi Sea is affected by that in Bering Sea, and to explain the relationship. The transfer entropy is a measure which identifies a nonlinear coupling between two random variables or signals and estimates causality using modification of time delay. We verified this measure checked a nonlinear coupling for simulated signals. With sea-ice concentration datasets, we found that sea-ice in Bering Sea is influenced by that in Chukchi Sea 3, 5, 6 months ago through the transfer entropy measure suitable for nonlinear system. Particularly, when a seaice concentration of Bering Sea has a local minimum, sea ice concentration around Chukchi Sea tends to decline 5 months later with about 70% chance. This finding is considered to be a process that inflow of Pacific water through Bering strait reduces sea-ice in Chukchi Sea after lowering the concentration of sea-ice in Bering Sea. This approach based on information theory will continue to investigate a timing and time scale of interesting patterns, and thus, a coupling inherent in sea-ice concentration of two remote areas will be verified by studying ocean-atmosphere patterns or events in the period.

Effects of pregnancy serum and scriptaid on development in early partheno embryo

오민기,정나현,김대승,윤종택 사단법인 한국동물생명공학회 2020 한국동물생명공학회지 Vol.35 No.2

Partheno Embryo's research is known to play a very important role in identifying the development of embryonic cells or analyzing the genetic mechanisms of embryonic development, but the information on apoptosis formed during the early stage of development on Partheno Embryo is very little. Therefore, this study analyzed whether the embryonic cell death of unit embryos can be inhibited by adding Scriptaid, one of HDACi, which plays a role in demethylation of histone proteins as a method of regulating the cell cycle in the early embryo development of Partheno Embryo. As a result, the differentiation rate was higher in the group that added Scriptaid and FBS, but the cellular development was higher in the group that added pregnant serum to Scriptaid. As a result of analyzing the expression of the gene through IF and PCR, the group with the addition of gestational serum increased the expression of BCL2 and PCNA, which affects the anti-Casp3 action in cell survival. In addition, it is interpreted that treatment of Scriptaid for 16 hours, rather than 24 h treatment lowers the expression of Casp-3, a representative factor of apoptosis, and also increases embryonic development, thus affecting early embryo development. Therefore, it is concluded that the 16-hour treatment of Scriptaid and the use of gestational serum will inhibit cell death in the early embryonic development and increase the development rate of the embryo.

문화적 특성을 고려한 공공도서관 도서 대출수요 분석 : 대구광역시 시립도서관을 사례로

오민기,김경래,정원웅,김건욱 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.3

기계학습을 활용한 남극의 지상기온 재구성 연구

오민기,김성중,전상윤 한국기상학회 2022 한국기상학회 학술대회 논문집 Vol.2022 No.4

중학교 과학 교과서 ‘자극과 반응’ 단원의 삽화 분석

오민기,정은영 제주대학교 교육과학연구소 2019 교육과학연구 Vol.21 No.1

The purpose of this study was to analyze illustrations in the unit ‘Stimulus and Reaction’ of middle school science. Illustrations presented in the unit ‘Stimulus and Reaction’ of middle school 9 science textbooks were analyzed in terms of their ‘Location’, ‘Type’, ‘Usage’, ‘Function’, ‘Reference’ and ‘Composition’ and then compared by subunits and textbooks. According to the analysis of textbook illustrations, the proportion presented in the body was the highest at 46.3%. The proportion of ‘Photo type’ illustrations was highest at 43.0% in the subunit ‘Sensory organs’. Each ‘Diagram type’ was highest at 29.6% and 38.5% in the subunit ‘Nerve system’ and ‘Hormones’. The ratio of illustrations to the use of ‘Data provision 1’ was high at 18.9% and 55.1% of ‘Data provision 2’ in terms of the function of illustration. And it was found that there were 30.8% of illustrations of ‘Decorative functions’ in the subunit ‘Sensory organs’. Also, each illustration of ‘Explanatory functions’ was highest at 32.1% and 35.8% in the subunit ‘Nerve system’ and ‘Hormones’. As for the reference of the illustration, ‘Non-reference’ was 85.5%, which was more common among all the subunit. The ‘Single composition’ of the illustrations was highest, which was 66.5%. And the proportion of ‘Partial enlargement’ was relatively high in the subunit ‘Sensory organs’. Also, the proportion ‘Paired’ was relatively high in the subunit ‘Hormone’. Considering these results, it is suggested to increase the rate of illustrations of ‘Explanatory’ or ‘Complementary’ functions in middle school science textbooks and specify the reference to the illustration in the body. By presenting the composition of the illustration as ‘Multiple’, it is necessary to help students to understand their learning contents in textbooks. 본 연구에서는 2009 개정 중학교 과학 교과서의 ‘자극과 반응’ 단원에 제시된 삽화를 분석하였다. 중학교 과학 교과서 9종의 ‘자극과 반응’ 단원에 제시된 삽화를 제시 위치, 종류, 용도, 기능, 참조 유무, 구성의 측면에서 분석하여 중단원별, 교과서별로 비교하였다. 교과서 삽화를 분석한 결과, 삽화가 본문에 제시된 비율이 46.3%로 가장 높았다. 삽화의 종류로는 ‘감각 기관’ 중단원에서 ‘사진’의 비율이 43.0%로 높게 나타났고, ‘신경계’와 ‘호르몬’ 중단원에서 ‘도해’의 비율이 각각 29.6%와 38.5%로 높게 나타났다. ‘자료제공’의 용도로 삽화가 제시되는 비율이 ‘자료제공 1’은 18.9%, ‘자료제공 2’는 55.1%로 높게 나타났다. 삽화의 기능 측면에서 분석한 결과, ‘감각 기관’ 중단원에서 ‘장식적’ 기능의 삽화가 30.8%로 많았고, ‘신경계’와 ‘호르몬’ 중단원에서 ‘설명적’ 기능의 삽화가 각각 32.1%와 35.8%로 가장 많았다. 삽화의 참조 유무로는 ‘비참조’의 경우가 85.5%였다. 삽화의 구성이 ‘단독’인 경우가 66.5%로 가장 높았고, ‘감각 기관’ 중단원에서는 ‘부분 확대’의 비율이 비교적 높았으며, ‘호르몬’ 중단원에서는 ‘짝’의 비율이 비교적 높게 나타났다. 이러한 결과를 고려할 때, 중학교 과학 교과서에서 ‘설명적’ 기능이나 ‘보충적’ 기능의 삽화 비율을 높이고, 삽화에 대한 참조를 본문에 명시하며, 삽화 구성을 ‘다중’으로 제시함으로써, 학생들의 학습 내용 이해에 도움을 줄 필요가 있다.

머신러닝을 활용한 기상조건에 따른 공공도서관 도서대출 수요분석

오민기,김건욱,신세영,이진명,장원준 한국디지털정책학회 2022 디지털융복합연구 Vol.20 No.3

Although domestic public libraries achieved quantitative growth based on the 1st and 2nd comprehensive library development plans, there were some qualitative shortcomings, and various studies have been conducted to improve them. Most of the preceding studies have limitations in that they are limited to social and economic factors and statistical analysis. Therefore, in this study, by applying the spatiotemporal concept to quantitatively calculate the decrease in public library loan demand due to rainfall and heatwave, by clustering areas with high demand for book loan due to weather changes and areas where it is not, factors inside and outside public libraries and After the combination, changes in public library loan demand according to weather changes were analyzed. As a result of the analysis, there was a difference in the decrease due to the weather for each public library, and it was found that there were some differences depending on the characteristics and spatial location of the public library. Also, when the temperature was over 35℃, the decrease in book loan demand increased significantly. As internal factors, the number of seats, the number of books, and area were derived. As external factors, the public library access ramp, cafe, reading room, floating population in their teens, and floating population of women in their 30s/40s were analyzed as important variables. The results of this analysis are judged to contribute to the establishment of policies to promote the use of public libraries in consideration of the weather in a specific season, and also suggested limitations of the study. 국내 공공도서관은 1, 2차 도서관 발전 종합계획을 토대로 양적 성장을 이루었으나, 질적으로는 다소 부족한 점이 있어 이를 개선하기 위한 다양한 연구가 수행되었다. 대다수 선행연구에서는 사회·경제적 요인과 통계분석에 한정되어 수행된 한계점이 있다. 이에 본 연구에서는 시공간적 개념을 적용하여 강우와 폭염으로 인한 공공도서관 대출 수요 감소를 정량적으로 산출하고, 기상 변화로 도서 대출 수요 감소가 높은 지역과 그렇지 않은 지역을 군집화하여 공공도서관 내·외부 요인들과 결합한 후 기상변화에 따른 공공도서관 대출 수요 변화를 분석하였다. 분석 결과 공공도서관별 기상으로 인한 감소 차이가 존재하였으며, 공공도서관의 특성과 공간적 위치에 따라 일부 다르게 나타났다. 또한, 기온이 35℃ 이상인 폭염일 경우 도서 대출 수요 감소 폭이 많이 증가하였으며, 랜덤포레스트 모형으로 분석한 결과 유의미한 요인이 도출되었다. 내적 요인으로는 좌석 수, 장서 수, 면적이 도출되었으며, 외적 요인으로는 공공도서관 접근 경사로, 카페, 독서실, 10대 유동인구, 30/40대 여성 유동인구가 중요한 변수로 분석되었다. 이러한 분석 결과는 특정 시즌 기상을 고려한 공공도서관 이용 활성화 정책 수립에 이바지할 것으로 판단되며, 연구의 한계점도 제시하였다.

중학교 과학 ‘자극과 반응’ 단원의 삽화 제시 유형에 따른 학습 효과

오민기,유형빈,정은영 全南大學校 師範大學 科學敎育硏究所 2018 科學敎育硏究誌 Vol.42 No.1

The purpose of this study was to investigate the learning effect of the illustration type in the unit ‘Stimulus and Reaction’ of middle school science. In order to do this, 2 types of learning materials were made; ‘Separation type’, in which the texts and illustrations are separated, and ‘Unity type’, in which the texts are integrated into the illustrations. The subjects of this study was 184 students in 6 classes in the 8th grade. ‘Separation type’ materials were applied to the 3 classes and ‘Unity type’ materials were applied to the other 3 classes for 4 lessons. The Group Embedded Figures Test was administrated to classify the learners according to the cognitive style, and the achievement test was administrated before and after lessons. The results are as follows. The group of ‘Unity type’ improved the achievement significantly compared with the group of ‘Separation type’. When ‘Unity type’ materials were applied to the learners with the field-independent cognitive style, the achievement was significantly improved, but there was no significant difference between the ‘Separation type’ and the ‘Unity type’ to the learners with field-dependent cognitive style. In the group of ‘Unity type’, the field-independent learners have significantly improved their achievement compared to the field-dependent learners. But there was no significant difference in the achievement of field-independent and field-dependent learners in the group of ‘Separation type’. In conclusion, ‘Unity type’ materials are more effective in improving achievement than ‘Separation type’ ones, and it is necessary to find another kind of learning materials for the learners with field-dependent cognitive style.