한국에서 발생한 돼지의 세균성 질병 조사

예재길,Yeh Jae-gil 한국임상수의학회 1994 한국임상수의학회지 Vol.11 No.2

The purposes of this investigations were survey of the occurred bacterial diseases, development of new animal health drug, guidance to formers on the treatment and control methods of diseases. Some series of investigations have been carried out by microbiological, pathological and serological examinations. The results could be summarized as follows. 1. A total of 953 cases of outbreaked swine diseases have been diagnosed in Clinical pathology laboratories, Bayer Vet Res Institute during 8 years (from 1986 to 1993). The high incidence diseases were colibacillosis, pleuropneumonia, streptococcal infection and pasteurellosis in decreasing order. 2. Pleuropneumonia caused by Actinobacillus pleuropneumoniae was the most important respiratory diseases and pasteurellosis by Pasteurella multocide could be confirmed in several cases. 3. Actinobacillus pleuropneumoniae 50 strains were isolated and identified by biochemical and serological tests. In serotyping test, 22 isolated strains were serotype 5, 21 strains as serotype 2, each 2 strains as serotype 3 and 7 by the coagglutination test. 4. Colibacillosis and edema discase caused by Escherichia coli has been the most predominant outbreaked disease in this investigations. The 100 isolates of E coli strains were sensitive to amikacin, colistin, enrofloxacin, gentamycin and trimethoprim -sulfamethoxazole. 5. Swine erysipelas caused by Erysipelothrix rhusiopathiae was confirmed 25 cases as acute septicemic forms. Isolates of E rhusiopathiae were highly sensitive to ampicillin, cephalothin, enrofloxcin, penicillin and tetracycline. 6. The 49 cases of hemorrhagic and necrotic enteritis in piglets were observed and 13 strains of Clostridium perfringens could be isolated and confirmed by biological and serological test. Isolates of Clostridium perfringens type C were highly sensitive to ampicillin, cephalothin, enrofloxacin, penicillin and trimethoprim- sulfamethoxazole. 7. The 14 strains of Streptococcus suis type II could be isolated from meningitis of piplets. 8. Polyserositis caused by Haemophilus parasuis and salmonellosis were observed and confirmed. Also Corynebacterial infections and several parasitosis have been also observed in this investigations.

국내(國內) 돼지에서 분리(分離)한 Erysipelothrix rhusiopathiae의 성장(性狀) 및 병원성(病原性) 관한 연구

예재길,석호봉,Yeh, Jae-gil,Seok, Ho-bong 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.2

In studies on Erysipelothrix rhusiopathiae infection of pigs, a series of investigations were conducted for isolation, biochemical properties, pathogenicity, serotyping and susceptibility to antimicrobial agents. The results could be summarized as follows: 1. A total of 14 isolates of E rhusiopathiae were made from an acute form of swine erysipelas and the incidence was high during the hot summer season. 2. The biochemical properties of the 14 isolates were identical to the reference strain of E rhusiopathiae. 3. Pathogenicity of the isolated strain(89368) were ascertained that $LD_{50}$ for mice was $1.8{\times}10 $ cfu and also typical urticarious lesions and acute septicemia for pigs were induced by intradermal and intravenous inoculation with isolates, respectively. 4. All isolates were serotyped by the agarose gel slide double-diffusion system and proved to be serotype 1. 5. In susceptibility test to antimicrobial agents, 14 isolates of E rhusiopathiae were highly sensitive to ampicillin, baytril, cephalothin, chloramphenicol, penicillin and tetracycline.

학술발표 연제 및 초록 : 경기지방에서 발생한 돼지 Aujeszky`s Disease Virus 감염병에 관한 연구

예재길(Jae Gil Yeh),박경윤(Kyung Yoon Park),권혁진(Heuk Jin Kwon),이창구(Chang Koo Lee) 한국수의공중보건학회 1991 예방수의학회지 Vol.15 No.4

Effect of Enrofloxacin (Baytril Premix)for the Treatment and Prevention of Respiratory Diseases in Swine

예재길,서정기,박경윤,김병기,김용희,Yeh Jae-Gil,Seo Jeong-Ky,Park Kyung-Yoon,Kim Byeung-Gie,Kim Yong-Hee The Korean Society of Veterinary Clinics 1992 한국임상수의학회지 Vol.9 No.2

돼지의 호흡기질병을 효과적으로 치료 및 예방하고자 Enrofloxacin(Baytril premix)를 사료에 혼합하여 호흡기질병이 산발적으로 발생하고 있는 돈군에 투여하여 호흡기질병의 치료여부, 증체량, 사료요구율, 폐병변치 등을 조사하여 다음과 같은 결과를 얻었다. 1. 육성돈군에 Enrofloxacin(Baytril premix)를 10일간 급여하였던 바 증체량은 33일동안 31kg이었으나 대조구에서는 20.5kg으로써 통계적 유의차를 보였으며(p<0.05), 사료요구율도 개선되었고(p<0.05), 호흡기질병의 임상증상도 없어졌다. 2. 폐병변조사에서 Baytril 급여구에서는 평균 폐병변의 형성정도는 전체 폐면적의 7.92%이었으나 대조구에서는 15.8%의 심한 병변이 형싱되어 있었다. 3. 공시농장에서 발생하고 있는 호흡기질병은 Actinobacillus pleuropneumoniae에 의한 흉막폐렴, Mycoplasma성 유행성폐렴 및 Pasteurella multocida에 의한 폐렴 등이었다. For the purpose of effective treatment and prevention of porcine respiratory diseases, Enrofloxacin(Baytril premix) was administered in the feed to trial herd occurred sporadically the respiratory diseases and then a serious investigation was carried out on the therapeutic effect, weight gain, feed conversion rate and lung lesion scoring. The results obtained were as follows, 1. The grower pigs which were administered with Baytril premix for 10 days gained the average body weight of 31kg for 33 days compared to 20.5kg of control group(p<0.05). Also drastical improvement of feed conversion rate(p<0.05) and disappearance of clinical signs were approved. 2. Average scorings of lung lesions were 7.92% in the medication group and 15.8% in the control group respectively. 3. Respiratory diseases outbreaked in the trial farm were pleuropneumonia caused by Actinobacillus (Haemophilus)pleuropneumoniae, Mycoplasmal pneumonia and Pasteurellosis.

Effect of Enrofloxacin on Post Weaning Diarrhea in Pig

예재길,서정기,김병기,이창구,한병우,Yeh Jae-Gil,Seo Jeong-Ki,Kim Byeung-Gie,Lee Chang-Koo,Han Byung-Woo The Korean Society of Veterinary Clinics 1991 한국임상수의학회지 Vol.8 No.1

돼지의 이유자돈 설사병을 효과적으로 치료, 예방하고자 Enrofloxacin(Baytril premix)을 사료에 혼합투여하여 설사 발생여부, 증체량, 사료요구율, 돈분내 대장균수의 변화, 분리한 대장균의 항균물질에 대한 감수성을 조사하여 다음과 같은 결과를 얻었다. 1. 이유자돈에 Baytril premix를 급여하였던 바 대조구보다 62일령에서 체중은 3.2kg의 통계적 유의차이를 보여(p<0.01) 현저한 증체효과 나타내었으며, 사료요구율도 계선되었고 설사발생도 예방할 수 있었다. 2. Baytril Premix를 급여한 자돈의 분변내 대장균수는 3.14$\times$$10^{8}$에서 5일후 2$\times$$10^{7}$ 개/g 10일후 1$\times$$10^{5}$개/g으로 현저히 감소하였다. 3. 공시농장에서 분리한 E. coli 10중의 항균물질에 대한 감수성은 baytril, gentamicin 및 trimeoprim+sulfamethoxazole에 감수성이 우수하였다.다. For the purpose of effective therapy and prevention of post weaning E. coli diarrhea, Enrofloxacin-(Baytril premix) was administered in the feed and a series of investigations were made the occurence of diarrhea, weight gain, feed conversion rate, changes In the counts of faecal E. coli and susceptibility of the isolated E. coli strains to antimicrobials. The results obtained were as follows 1. The weaned piglets which were treated with Baytril premix gained the average body weight of 3.2kg over the control group at the age of 62 days. The result indicates a marked weight gain effect which, by statiscal analysis, showed a significant difference(P<0.01). Administration of Baytril premix also resulted in the improvement of feed conversion rate and provention of post weaning diarrhea. 2. The counts of faecal E. coli in Baytri premx treated piglets showed a marked decrease from 3.14${\times}$10$\^$8/cells/g to 2${\times}$10$\^$7/cells/g at 5days post treatment and 1${\times}$10$\^$5/cells/g at 10 days post treatment. 3. 10 strains of E. coli isolated fred the experimental pig farm were highly susceptible to Bayoil, gentamicin and trimethoprim plus sulfamethoxazole.

국내(國內) 돼지의 Clostridium perfringens type C 감염증에 관한 연구

예재길,박경윤,조성근,Yeh, Jae-gil,Park, Kyoung-yoon,Cho, Soung-kun 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.3

Thirteen strains of Clostridium perfringens were isolated from the pigs with hemorrhagic enteritis. The characteristics of the outbreaks, clinical signs and lesions were examined. The biochemical properties, type of toxin and susceptibility to antimicrobial agents of the isolates were investigated. The results could be summarized as follows ; 1. Almost of the pigs affected with hemorrhagic enteritis, 17 cases examined from 1989 to 1992, were piglets less than 7 day old. 2. The average mortality rate of piglet less than 7 day old affected with hemorragic and necrotic enteritis was 48.5%. 3. The clinical signs of pigs with hemorrhagic enteritis were depression, hemorrhagic diarrhea, anemia and dehydration. Necropsy of the infected pigs showed typical hemorrhage of upper intestine and necrosis of mucosal membrane. 4. The characteristic biochemical properties of the isolates were 2-band hemolysis, positive reaction of reverse CAMP test and formation of LV precipitate in egg yolk medium. 5. The toxin type of the 13 isolates, investigated by mouse inoculation test, was all type C strains of Clostridium perfringens. 6. In susceptibility test to antimicrobial agents, 13 isolates of Clostridium perfringens were highly sensitive to ampicillin, enrofloxacin(Baytril), cephalothin, penicillin and trimethoprim-sulfamethoxazole.

돼지 parvovirus불활화(不活化) vaccine 및 중화항체가측정(中和抗體價測定)에 관한 연구(硏究)

권혁진,예재길,이창구,Kwon, Hyock-jin,Yeh, Jae-gil,Lee, Chang-koo 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2

A porcine parvovirus inactivated vaccine was prepared and inoculated to 7 piglets and also 8 guinea-pigs, and their serum antibodies were titrated. Twenty-two field serum samples of unvaccinated sows were also tested by SN and HI methods. It was observed that SN test was superior over HI test. Therefore, it is suggested that the SN test could well be used in the detection of serum antibody for PPV in vaccinated pigs.

'83 돈(豚)콜레라 유행시(流行時)의 면역모돈(免疫母豚)과 자돈(仔豚)의 END혈청중화항체가(血淸中和抗體價) 조사(調査)

전윤성,예재길,서익수,Jeon, Yun-seong,Yeh, Jae-gil,Seo, Ik-soo 대한수의학회 1985 大韓獸醫學會誌 Vol.25 No.1

Hog cholera serum neutralizing antibody of piglets and sows were titrated by means of END SN method. The piglets of a variety of ages, precolostrally immunized with LOM living HC vaccine were subjected to the test. The sows were vaccinated with lapinized living HC vaccine after 25 days from the parturition. Throughout the studies the following results were obtained and summarised. 1. Hog cholera antibody titers of inbred sows immunized with lapinized living HC vaccine after 25 days from parturition were high except Hampshire group(Table 2). 2. Sows, different stage of the pregnancy or the day of parturition, and of 3 way crossed, that were immunized with lapinized living HC vaccine have shown no significant difference on HC antibody titer(Table 2, 3). 3. HC antibody titers of piglets, immunized with a single dose of LOM HC vaccine before feeding colostrum, were high in case of the younger group(1 week) compare to the older(7 week) (Table 4). 4. The piglets that were booster immunized with LOM HC vaccine at the age of 7 weeks have shown an inconsistent antibody titers(Table 5).

국내(國內) 닭의 괴사성 장염 발생에 관한 연구

박경윤,정성대,예재길,서일복,Park, Kyoung-yoon,Jung, Sung-dae,Yeh, Jae-gil,Seo, Il-bok 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.3

This study was carried out to investigate the necrotic enteritis of chicken in Korea. Epidemiological, pathological and microbiological examination were done on 12 naturally occurred cases of necrotic enteritis of chicken. And the susceptibility of isolated bacteria to antimicrobial agents was also examined. The results obtained were as follows; 1. The Clostridium perfringens infection, necrotic enteritis of chicken, occurred in the chickens mainly raised in floor pens. Necrotic enteritis of chicken was occurred coincidently with coccidiosis and Gumboro disease frequently. And several cases were recurred at 2-3 weeks after recovery. 2. Clinical signs of the infected chickens were depression, decreased appetite, reluctance to move, diarrhea, ruffled feathers and acute death within several hours. 3. The characteristic biochemical properties of isolates were 2-band hemolysis, no motility, positive reaction of reverse CAMP test and the formation of LV precipitate in egg yolk medium. 4. Gross lesions of the infected chickens were distention of intestine with gas, thickened mucosa and formation of thick pseudomembrane in intestine. Livers were friable with yellowish brown color and, in some case, showed demarcated necrotic foci. 5. Histopathological findings of the infected chickens were severe necrosis of the intestinal mucosa and attachment of numerous large bacilli to the mucosal surface of necrotic villi. In liver, necrosis of liver tissue and numerous large bacilli in the necrotic foci were also observed. 6. In susceptibility test to antimicrobial agents, 12 isolates of Clostridium perfringens were highly sensitive to ampicillin, baytril, cephalothin and penicillin.

자돈에서의 연쇄구균성 감염증에 관한 연구 I. 이환돈에서의 streptococcus suis type II에 의한 돼지뇌막염의 실태와 약제 감수성

석호봉(Ho Bong SEOK),예재길(Jae Gil YEH) 한국예방수의학회 1990 예방수의학회지 Vol.14 No.1

Investigations into the bacteriology and epidemiological features of streptococcus suis typeⅡ infections, especially in naturally-occurring field outbreaks of meningitis and its carrier state were carried out from June 1987 to December 1989 at intensive pig farms in parts of Seoul, Kyounggi, Chungchong and Kyoungnam areas. Also determined were isolated frequency of streptococci in various sources of effected pigs and antibiotics susceptibilities of the isolates. The results obtained were summarized as follows: 1. Streptococci were isolated from porcine specimen submitted to the lab. from affected pig farms and 48 strains isolated from pigs of different clinical symptoms belonged to 14 brains, 23 lungs and 11 feces. 2. The brain diseased pigs ranged mostly from 30 to 50 days of age and main clinical symptoms of the disease were CNS sign and meningitis. The morbidity and mortality of this pig weaned in a part of pig farms were 4 to 10 and 30 percent, respectively. 3. Most of brain-origin streptococci(78.7%) was belonged to Lancefield group D and identified as Streptococcus suis typeⅡ by biological and additional serological examinations. Other 3 strains were divided to group C, group F and group G.