광해지 식생복원을 위한 하수슬러지 이용 효과

염규진(Kyu Jin Yum),김판기(Pan Gi Kim),박은우(Eun Woo Park) 大韓環境工學會 1999 대한환경공학회지 Vol.21 No.12

상향류식 바이오비드 공법을 이용한 오,폐수 처리특성 및 부착 생물막의 형태적 특징

염규진 ( Kyu Jin Yum ),이정훈 ( Jeong Hun Lee ),김선미 ( Sun Mi Kim ),최원석 ( Weon Seok Choi ) 한국물환경학회 2002 한국물환경학회지 Vol.18 No.2

The objective of this study was to investigate the treatment efficiency, kinetics, and morphological characteristics of biofilm in upflow Biobead^ⓡ process, a kind of biological aerated filter(BAF). The Biobead^?? system showed high removal rates of COD_Mn, (76∼83%), BOD_5(67∼88%) and SS(71∼91%) for food wastewater with high salt concentration (>4,000㎎/ℓ) under short reaction times(2∼3hrs). Even at aerobic condition, the system had high treatment efficiency for both T-N (51∼63%) and T-P(62∼81%). The removal kinetics of COD_Mn, BOD_5, T-N, T-P, and Cl^- in the Biobead^?? system showed a plug-flow pattern with reaction rate constants(hr^-1) of 0.58, 0.63, 0,30, 0.48, and 0.38 respectively. A backwashing process to remove excess biomass and filtered solids was needed at least once during 22-hour operation at 0.5kg BOD m^-3·d^-1 loading. At the higher loading(1.0kg BOD m^-3·d^-1) the backwashing interval was shorten by 8 hours. The COD, BOD, T-N, and T-P were removed from 43 to 66% only by aerobic biodegradation. The SS was removed over 70% by the filtering of Biobead^?? media in the treatment system. The first one of three serial Biobead reactors showed the highest removal values for COD_α(52.3%), COD_Mn(38.8%), BOD(62.5%), and T-N(40.0%). The SS and T-P had the highest removal values(47.5% and 29.2%) at the second one of the serial reactors. The biofilm had non-homogeneous spatial distribution and the colonies were embedded in the sunk area of the Biobead. The thickness of the biofilm was very thin (5.0∼29.4㎛) compared to the biofilm thickness(200∼300㎛) used in other BAF systems

대구시 5 종 사업장 원폐수의 특성 분석

이태관(Tae Gwan Lee),염규진(Kyu Jin Yum) 한국수처리학회 1998 한국수처리학회지 Vol.6 No.4

In this study, we examined wastewater from small businesses in Taegu. Small businesses might be minor sources of pollution, but they are potentially dangerous because of their toxic substances and poor wastewater treatment. There are no special policies or programs for the small business in Korea. Therefore we focused our investigation of wastewater to propose environmental policy for the small business. We chose some small businesses three plating factories, three dyeing factories, three transportation companies and one chemical factory all located in the Sungser industrial complex and 10 car washes randomly selected throughout Taegu, and then examined their wastewater. Our results found, the characteristics of wastewater from each of the factories were totally different and wastewater from the car washes had harmful quantities of E-coli and ABS which are omitted from present day regulation. Our findings support the assertion that Korea needs broader and specialized policies for various kinds of small businesses.

열무김치에서 발굴한 Saccharomyces servazzii의 신규 Strain인 Ceb-kc-011의 생육조건 최적화 및 서목태 생물전환에 따른 α-Glucosidase 억제 효능

박찬호(Chan-Ho Park),최예림(Yae Rim Choi),염규진(Kyu-Jin Yum),김민정(Min Jung Kim) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.3

본 연구는 열무김치로부터 신규 효모를 분리 및 동정하였고 배양조건을 최적화시킨 후 효모를 활용해 서목태를 생물전환함으로써 α-glucosidase 억제 활성을 확인하였다. 열무김치에서 18S rRNA 염기서열 분석으로 선정된 균주는 S. servazzii Ceb-kc-011로 명명하고, 배양조건은 YPD 배지, 25°C, 호기배양으로 최적화되었다. YPD 기본배지에서 질소원을 서목태로 대체했을 때에는 S. servazzii Ceb-kc-011의 생장속도는 YPD 배지에서 배양했을 때보다 적었으나, 서목태 대사산물에 의한 α-glucosidase 억제능은 더 높아진 것으로 나타났다. 질소원을 서목태로만 설정한 상태에서 서목태 함량을 0.2~4.0% 변화를 시켜주면서 α-glucosidase 억제능을 살펴본 결과 서목태 함량이 0.6~1.0%에서 유의적으로 최고의 억제능이 나타났다. 또한 서목태 함량 0.6% 조건에서 생성된 서목태 대사산물의 α-glucosidase 활성에 대한 IC50은 29.5 mg/mL로 나타났다. α-Glucosidase 억제는 항비만, 항당뇨 소재 개발에 있어서 가장 기초적인 시험법이기 때문에, 이러한 결과는 효모로 서목태를 생물전환함으로써 생성된 서목태 대사산물을 항비만, 항당뇨 소재로의 개발 및 응용에 활용할 수 있을 것이라 예상된다. Food bioconversion and fermentation is a process wherein microorganisms convert food materials to degradation products, including organic acids and bioactive substances. Rhynchosia nulubilis, which is used traditionally as a food and herbal medicine in Asia, has also been biotransformed by microorganisms, and fermented R. nulubilis has anti-hyperglycemic, anti-hyperlipidemic, and anti-obesity activity. On the other hand, the microbes involved in the fermentation of R. nulubilis remain unknown. In particular, the role of yeast in inducing the biotransformation of R. nulubilis has yet to be examined. This study assessed the adaptation of yeast isolated from young radish (Yeolmoo) kimchi for inducing bioconversion using R. nulubilis. A novel yeast strain was isolated from young radish kimchi and called Saccharomyces servazzii Ceb-kc-011 by 18S rRNA gene sequence analysis. The results showed that the optimal culture conditions for Ceb-kc-011 were YPD media [yeast 1% (w/v), peptone 2% (w/v), D-glucose 2% (w/v)], 25°C, and a 24-hour incubation time under aerobic conditions. When R. nulubilis was added to the media, the growth of S. servazzii Ceb-kc-011 was indicated by the presence of yeast and peptones. Still, bioactive substances were produced in the absence of yeast and peptones (2% D-glucose and 4% R. nulubilis). Bioconverted R. nulubilis inhibited α- glucosidase activity (41.43±0.25%). In conclusion, the bioconversion of R. nulubilis using a specific microorganism was observed, highlighting its potential for the production of anti-diabetic agents through bioconversion.

Endosulfan으로 오염된 현장토양의 복합미생물제제를 이용한 생물학적 정화

박찬호(Chan-Ho Park),이주영(Joo-Young Lee),조민지(Min-Ji Cho),성금화(Jinhua Cheng),염규진(Kyu-Jin Yum) 대한환경공학회 2020 대한환경공학회지 Vol.42 No.11

목적: 잔류성 유기염소계 농약의 사용은 금지 되었지만 여전히 많은 농경지에서 검출되고 있다. 본 연구에서는 다양한 후보 미생물 중에서 최종 선발된 Phanerochaete chrysosporium Y-2 (KCCM-10725P) 및 Streptomyces sp. MJM14747 (KACC 81078BP) 균주를 복합미생물 형태로 현장토양에 적용하여 endosulfan 제거율을 평가하였다. 방법: 현장 시험용 부지는 대조구 및 시험구로 각각 3 m × 3 m 면적으로 설정하였다. 두 site에 35% endosulfan 유제를 살포하여 최종 농도가 약 20 ㎍/g이 되도록 하였다. 그 후 아무 처리를 하지 않은 톱밥 12 kg 및 복합미생물이 담지된 톱밥 12 ㎏을 두 site 토양에 각각 혼합하였다. 총 100일 동안의 실험 기간 중 시료는 30일 단위로 채취하였다. 채취된 토양 시료는 QuEChERS AOAC 방법에 따라 추출 및 전처리하였고 gas chromatography로 분석하였다. 결과 및 토의: 대조구 및 실험구에 endosulfan 유제를 처리한 직후의 농도는 각각 21.2 ㎍/g 및 21.9 ㎍/g으로 분석되었다. 미생물을 처리하지 않은 대조구 사이트의 endosulfan 제거율은 30일까지 약 21.9%를 나타냈고, 100일째에는 54.4%를 나타냈다. 반면, 복합미생물을 처리한 사이트의 100일째 endosulfan 제거율은 89.5%로 대조구에 비하여 1.6배 높게 나타났다. 30일째의 제거율은 56.8%였고 이후 endosulfan의 제거율이 빠르게 증가하는 경향을 보였다. Endosulfan의 주요 성분 중 α-endosulfan의 제거율이 β-endosulfan보다 높게 나타났다. 100일 경과 시 α-endosulfan과 β-endosulfan의 제거율은 각각 98.0% 및 83.5%로 확인되었다. 결론: 미생물복합제제로 선발된 Streptomyces sp. MJM14747은 100 ㎍/g 내외의 고농도 endosulfan 존재하에서도 분해능이 우수하였고, Phanerochaete chrysosporium Y-2은 endosulfan의 분해속도가 느린 β-endosulfan의 제거에 우수하였다. 복합미생물제제는 앞으로 잔류농약 문제에 직면해 있는 국내외 농경지 토양뿐만 아니라 생물학적 정화사업 전반에 응용 가능할 것으로 기대된다. Objectives : The use of risky and persistent organochlorine pesticides was prohibited, but is still detected in many agricultural lands. In this study, Phanerochaete chrysosporium Y-2 (KCCM-10725P) and Streptomyces sp. MJM14747 (KACC 81078BP), which were finally selected among various candidate microorganisms, were applied to field soil and estimated the endosulfan removal rate. Methods : The field testing sites were set to an area of 3 m × 3 m, respectively. A 35% endosulfan emulsion was sprayed on both sites so that the final concentration was about 20 ㎍/g. Thereafter, 12 ㎏ of original sawdust and 12 ㎏ of sawdust loaded with microbial consortia were mixed at each of the two sites. During the test period for a total of 100 days, samples were taken every 30 days. Soil samples were extracted according to the QuEChERS AOAC method and analyzed by gas chromatography. Results and Discussion : The concentrations immediately after endosulfan emulsion treatment at both sites were detected as 21.2 ㎍/g and 21.9 μg/g, respectively. The removal rate of endosulfan at the site without microbial consortia treatment (Control) was about 21.9% until the 30th day, and 54.4% on the 100th day. On the other hand, the endosulfan removal rate on the 100th day of the site treated with microbial consortia was 89.5%, which was 1.6 times higher than that of the control site. On the 30th day, the removal rate was 56.8%, and the rate of degradation of endosulfan increased thereafter. Among the major components of endosulfan, the removal rate of α -endosulfan was higher than that of β-endosulfan. After 100 days, the removal rates of α-endosulfan and β -endosulfan were 98.0% and 83.5%, respectively. Conclusions : Streptomyces sp. MJM14747 showed good degradation ability in the presence of endosulfan at a high concentration of around 100 ㎍/g. Moreover, Phanerochaete chrysosporium Y-2 has a good performance in the removal of β-endosulfan, which is slow degrading components of endosulfan. Microbial consortia are expected to be applied not only to domestic and foreign agricultural land but also to overall bioremediation projects.

태백지역 석탄 폐석지의 식생 복원을 위한 향토 수종 선발

이재천,한심희,장석성,이정환,김판기,허재선,염규진 한국농림기상학회 2002 한국농림기상학회지 Vol.4 No.2

This study was carried out to identify the relationship between naturally introduced vegetation on the abandoned coal mine lands and the surrounding forest, and to select the indigenous tree species for the revegetation of the coal mine lands in Taeback, Kangwon Province, Korea. The apparent species in the surrounding forest of Sododong were 9 species of tree layer, 13 species of subtree layer, 23 species of shrub layer and 23 species of herb layer, respectively. The dominant species in Sarijae were 6 species of tree layer, 15 species of subtree layer, 31 species of shrub layer and 25 species of herb layer, respectively. The plant species in the abandoned coal mine land were 31 species in Sododong and 30 species in Sarijae, respectively. The number of plant species in the abandoned coal mine land was smaller than the surrounding forest. The common species of the coal mine lands and the surrounding forest were 6 species(Betula schimidtii etc.) in Sododong and 4 species(B. costata etc.) in Sarijae. It was considered that the species in the coal mine lands introduced from the species of top layer in the surrounding forest. Especially, B. costata in Sarijae showed the edge effect, which regenerates the vegetative community from the boundary area of coal mine land and surrounding forest. Natural revegetation in Sododong was also progressing by the invasion of tree species in surrounding forest, such as B. schmidtii. Consequently, natural revegetation in the disturbed coal mine lands may be related to the species composition of the surrounding forest.