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구강암 발생 과정에서 TGF-α 및 TGF-β 발현에 관한 연구
양희창,이동근 원광대학교 생체재료·매식연구소 1997 원광생체재료·매식 Vol.6 No.1
Though many genetic and epigenetic alterations have been identified in hamster oral carcinogenesis model, there is no information about the possible role of transforming growth factor related with oral cancer. The purpose of this paper was to find the expression patterns of transforming growth factor alpha and beta during the stages of complete oral carcinogenesis model in hamster. 0.5% 9, 10- dimethyl-1, 2-benzanthracene(DMBA) in mineral oil was topically applied to the buccal pouch of 75 hamsters three times a week during the experimental periods. The experimental animals were subdivided into two groups of control and experiment. Only the mineral oil was applied to the control group. 0.5% DBMA in mineral oil was applied to the experimental groups of 6,8,10,12,14,16,18 and 20 weeks. The expression of the TGF-α and TGF-β protein were evaluated by the distribution and intensity of positive cells during the carcinogenesis using the immunohistochemical study. The following results were obtained; 1. The buccal pouch epithelium of hamster was histologically changed to the dysplasia at 6, 8, 10 weeks, carcinoma in situ at 12 weeks, and squamous cell carcinoma at 14 weeks. 2. The expression of the TGF-α was restricted to the parabasal and basal layers of the normal and dysplastic mucosa, but those positive cells were extended to the spinous layers of the epithelium in the carcinoma. 3. The degree of TGF-α expression was markedly decreased in the carcinoma at 16, 18, 20. The strong positive staining in the center of cancer islands and weak positive staining in periphery of tumor were seen at the stage of squamous cell carcinoma 4. The positive index of the TGF-α had a tendency to increase with DBMA- applied time. There was a statistically significant difference between 12, 18, 20 experimental group and control group (p<0.05). 5. The expression of the TGF-β was shown at the cytoplasm of all control and experimental groups, and the parabasal and basal layers of the normal and dysplastic mucosa, but it was shown at the basal layers of the epithelium in the carcinoma. 6. TGF-β was expressed diffusely at 16, 18, 20 experimental group. The strong positive staining in the center of cancer islands and positive staining in periphery of tumor were seen at the stage of squamous cell carcinoma From the above findings, the expression of TGF-α and β in oral carcinogenesis model seems to have two formal stages, the first being an overexpression step as reaction to uncontrolled growth and the second being one in which external protein accumulate in the surrounding stroma and intracytoplasm. Overexpression of TGF-α and β may have important cooperative roles for the promotion of cancer and factor of prognosis.