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4-Pentenoic Acid로 유발시킨 라이증후군 흰쥐의 뇌에서 GFAP면역반응성 성상교세포의 변화
양현억, 황경태(Hyun-Eog Yang, Kyung-Tai Whang) 대한소아신경학회 1996 대한소아신경학회지 Vol.3 No.2
4-pentenoic acid(4-Pa)로 유발시킨 실험적 라이증후군 흰쥐에서 교세포원섬유성 산성단백질(GFAP)항체를 이용한 광학 및 전자현미경적면역세포화학법으로 대뇌와 소뇌의 피질 및 수질에 분포하는 GFAP양성 성상교세포를 감별 염색하여 그 면역반응성과 이들이 차지하는 면적 비율을 계측하여 성상교세포에서의 GFAP활성도를 확인하고, 아울러 이들 미세구조적 변화를 관찰하여 다음과 같은 결과를 얻었다. 4-PA를 투여한 동물에서 투여후 40-80분 사이에 모두 경련발작을 일으켰으며, 4-PA투여후 30분에 혈당값은 정상대조군 것에 비하여 유의하게 감소하였고, SGOT값과 혈중 암모니아값은 정상대조군 것에 비하여 유의하게 증가하였다. 4-PA를 투여한 동물의 대뇌에서 대상속에 분포하는 GFAP양성 성상교세포의 GFAP면역반응성과 면적비율은 정상군 것에 비하여 다같이 유의한 증가를 나타내었다. GFAP항체를 이용한 면역전자현미경적 관찰에서 볼 때 성상교세포의 세포질에 있는 중간세사가 선택적으로 염색되었으며, 이들 중간세사는 4-PA투군의 대뇌 및 소뇌 성상교세포에서 현저히 증가하였다. 이상의 관찰결과로 미루어 볼 때 4-PA에 의한 실험적 라이증후군에서 조기에 대뇌 및 소뇌에 분포하는 성상교세포의 GFAP발현과 중간세사가 현저히 증가하며, 이와 같은 현상은 라이증후군에서 대상적 반응성 성상교세포중의 한 양상으로 생각된다. Background: 4-pentenoic acid(4-PA) has been known to be a useful neurotoxin to produce most of the essential features of Reye's syndrome in rats, but little has been studied about the effects of this reagent on the changes of the glial fibrillary acidic protein(GFAP) immunoreactivity of astrocytes in the central nervous system. We designed this study in order to clarify the alterations of the GFAP-immunoreactivity, area percent and ultrastructure of GFAP-positive astrocytes in the brain of the rat with experimental Reye's syndrome by light and electron microscopic immunocytochemistry using anti-GFAP antiserum. Method: Reye's syndrome was induced by intraperitoneal injection every 4 hr with 50mg/kg body weigh of 4-PA for 10 doses, followed by a single dose of 200mg/kg. Animal were sacrificed in 30 minutes after th final injection, and tissue blocks were taken from the cerebrum and cerebellum. Paraffin sections were immunostained with the indirect immunoperoxidase method for light microscopy, and the area percent of GFAP-positive astrocytes was calculated using the image analysis system(Vidas 2.0, Kontron, Germany) in the immunostained sections. For electron microscopy, pre-embedding immunocytochemical method was applied Result: During the experimental period, all rats were observed clinical features similar to those of the Reye's syndrome, including convulsion, hyperammonemia, hypoglycemia and elevated SGOT. The GFAP immunoreactivity and the area percent of GFAP-positive astrocyte in 4-PA-treated animals were significantly increased in cerebral cingulum and cerebellar cortex and medulla, as compared with those of normal control. The increment was highly signigicant in cerebellum than in cerebrum. However, the intensity was observed to be slight in frontal cortex. The intermediate filaments in the cytoplasm of astrocytes were selectively immunostained by the electron microscopic immunocytochemistry and these GFAP-immunoreactive intermediate filaments were markedly increased in both cerebral and cerebellar astrocytes of the 4-PA treated animals. Conclusion; These observations elucidate that the increment of GFAP expression and intermediate filaments in the astrocytes of cerebrum and cerebellum are striking feature in the early stage of Reye's syndrome induced by 4-PA, and suggest that the compensatory reactive astrocytosis is consequently involved in the encephalopathy.
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경기북부 지역의 Richettsia 질환에 관한 임상적 고찰
전성주(Seoung Ju Chun),이기영(Kee Yeoung Lee),최두혁(Du Hyok Choi),양현억(Hyun Eog Yang) 대한내과학회 1989 대한내과학회지 Vol.36 No.4
N/A Rickettsiosis is one of the most prevailng acute febrile diseases in the northern part of Kyoung Ki Do during harvest-time. We experienced 36 cases of rickettsiosis in this area during 1978. Herein we report the epidemiological and clinical observations with laboratory findings. 1) The disease was prevalent in adult female farmers and the epidemic seasons were fall and early winter. 2) The serologic test revealed a positive rate of 67% (24 cases) in R. tsutsugamushi and 33% (12 cases) in R. typhi and two cases were seropositive to Korean Hemorrhagic Fever. 3) Clinical pictures of Tsutsugamushi disease were characterized by fever (88%), chills (71%), headache (71 %) and abdominal pain (50%) but were less severe in R. typhi as fever in 83% of cases, chills 67% and headache 67%. 4) On physical examination, abdominal tenderness (54 %), fever (38%), CVA tenderness (29%), tachycardia (25 %) and eschar (13%) were found but were less severe in R. typhi as abdominal tenderness in 25% of cases, fever 25%, CVA tenderness 17% and tachycardia 17%. 5) On laboratory examination, a left shift of leukocytes, atypical lymphocytes, anemia, microscopic hematuria and proteinuria were found. Blood chemistry findings were characterized by increased SGOT & SGPT and increased CPK & LDH. The chest X-ray revealed interstitial pneumonia in nine cases (25%).
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