아토피 피부염의 한약 치료에 대한 무작위대조군연구 중심의 연구 동향 - CNKI를 중심으로

양금진,조은희,강수진,홍지은,심유화,이주현,박민철 한의병리학회 2020 동의생리병리학회지 Vol.34 No.2

The purpose of this study was to analyze the research trends of randomized controlled trials on herbal medicine treatment for atopic dermatitis in China for the last 5 years. We searched for randomized controlled trials with the intervention of herbal medicine for the treatment of atopic dermatitis in the CNKI (China National Knowledge Infrastructure) from January 2014 to December 2018. For the screening of the paper, we used ‘特应性皮炎’ and ‘异位性皮炎’ which mean atopic dermatitis and search was limited to three areas within Medicine & Public Health: Traditional Chinese Medicine, Traditional Chinese Medicinal Herbs, Combination of Traditional Chinese Medicine with Western Medicine. Among the 136 searched studies, we selected a total of 34 studies and analyzed a year of publication, subject characteristics, study design and intervention, prescribed herbal medicine and herbs, pattern identification, evaluation criteria, and outcomes. Longmu decoction (龍牡湯) and Polia Sclerotium (茯笭) was the most frequently prescribed medicine and herb. The most commonly used pattern identification was Blood deficiency and Wind-dryness (血虛風燥), and among them, the most frequently prescribed herb is Rehmanniae Radix (生地黃). In most studies using the total effectiveness and SCORAD index as an outcome measure, the herbal medicine treatment group showed statistically better results than the control group. As a result of the safety assessment, the herbal medicine treatment group was reported having significantly fewer side effects compared to the control group. Hence, it was confirmed that the intervention including herbal medicine had a significant effect on atopic dermatitis. This study would be able to provide the basis of clinical research on atopic dermatitis and applied to the treatment of atopic dermatitis.

Identification and Characterization of Neurotrophic Factors in Porcine Small Intestinal Submucosa

양금진,박기철,Hyun Su Choi,Jun-Hyuk Choi,박상열,이일우,이형진 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.5

“Small intestinal submucosa” (SIS) is a natural degradable biomaterial derived from the small intestineof vertebrates. Porcine SIS has been widely used in repairing various tissues and organs, especially in nervous tissues because intrinsic autonomic nerve plexuses are included in submucosal layer. However, little is known about active neurotrophic factors in this material. In this study, we proposed a method to prepare an extract from decellularized SIS and to identify and characterize the major neurotrophic factors in it. Decellularized SIS extract was obtained by sequential procedures: mechanical disassociation, degreasing, enzyme digestion, freeze-drying, freezer-milling,extracting, concentrating, and filtering. Contamination with cellular components in the SIS extract was evaluated by hematoxylin and eosin (H&E) staining and the content of genomic DNA. Major neurotrophic factors in SIS extract was assessed quantitatively by ELISA. The effects of SIS extracts on cells were evaluated by observation of neurite outgrowth from PC12 cells. Decellularized SIS extract was obtained successfully by the proposed method and no cellular component was found within it. Brain-derived neurotrophic factor (BDNF), erythropoietin (EPO), glial cellderivedneurotrophic factor (GDNF), nerve growth factor (NGF), and platelet-derived growth factor (PDGF) were identified and characterized form SIS extract. The bioactivity of SIS extract was assessed in terms of neurite outgrowth from PC12 cells. In summary, we prepared a decellularized extract from porcine SIS and identified major neurotrophic factors present in it. SIS extract also had bioactivity with PC12 cells. From these results, we conclude that SIS may be useful to repair or improve nervous system function.

인간염색체 12q13에 내재한 마우스 Gamm1의 인간유전자 homolog, MYG1의 클로닝과 발현

양금진(Keumjin Yang),이형남(Hyoungnam Lee),배윤정(Younjung Bae),신동직(Dongjik Shin),김은민(Eunmin Kim),윤종복(Jong-Bok Yoon),박영일(Youngil Park),김준(Jun Kim),유지창(Jichang Yu),김성주(Sungjoo Kim) 한국생물공학회 2002 KSBB Journal Vol.17 No.4

수술 환자의 안전 증진 강화 활동

조은정,강현주,양금진,이효순,노숙자,최혜영,노미라,주유미,최영지,오유경,한유정,최지영,김금미,전지혜,김신혜,장미영,송선미,봉은정,오은녀,오미란,문미현 한국의료QA학회 2009 가을학술대회 Vol.2009 No.3

체계적인 교육제공을 통한 학생 간호사의 실습 만족도 향상 활동

문춘심,명선희,양금진,최은선,이강순,최설희,이은선,정세미 한국의료QA학회 2009 가을학술대회 Vol.2009 No.2

유방암 환자의 항암화학요법 후 한방병원 입원치료에 대한 일개 한방병원 환자 특성 및 치료 고찰

강수진,김선경,양금진,홍가경,조한백 대한한방부인과학회 2020 大韓韓方婦人科學會誌 Vol.33 No.2

Objectives: The purpose of this study is to analyse current status and treatment of the Korean medicine hospital after chemotherapy in patients with breast cancer. Methods: We investigated the medical records of 21 patients who admitted to Korean medicine hospital after chemotherapy in patients with breast cacner from March 1, 2017 to December 31, 2019. We searched medical records retrospectively and analyzed current status and treatment of Korean medicine hospital. Results: The average age of 21 participants was 52.81±8.38 years and 40s and 50s accounted for 85.6% of the total. After receiving chemotherapy, the average time to hospitalization was 1.87±3.13days and average hospital stay was 9.78±4.14 days. The subjects were classified as 28.6% of stage I, 52.4% of stage Ⅱ, 9.5% of stage Ⅲ, and 9.5% of stage Ⅳ. The analysis according to the presence of metastasis was 57.1% without metastasis, 33.3% with axillary lymph node metastasis, and 9.5% with distant metastasis. The main symptoms complained when hospitalized by 21 subjects were nausea (54.2%), fatigue (54.2%), and anorexia (50.8%) in over 50%, pantalgia (47.5%), and insomnia (47.5%), dizziness (44.1%), cold sweating (42.4%), lower extremity pain (40.7%), 37.5~37.9℃ fever (39.0%), headache (37.3%), hot flush (37.3%), pruritus (30.5%) are 30% or more. Korean medicine treatment was performed in 87.4% of all hospitalizations and Gwakhyangjunggi-san-gami was the most administered prescription. Extracts of Korean medicine was performed in 100.0% of all patients and Eunkyo-san was most administered extracts medicine. Acupuncture, moxibustion, and cupping treatments were performed in all 21 study subjects. Other treatments was performed at a frequency of hyperthermia (90.5%), lymph massage (23.8%), air compression therapy (23.8%), and Interference current therapy (19.0%) Conclusion: Korean traditional medicine can be used as a countermeasure for side effects after chemotherapy in breast cancer patients.

Effect of leptin receptor deficiency on spermatogenesis in NOD mice

이철호,강길태,양금진,황정환,김용훈,노정란 한국실험동물학회 2010 한국실험동물학회 학술발표대회 논문집 Vol.2010 No.2

발열을 동반한 호중구감소 상태의 급성백혈병 환아에서 경험적 항진균제로 투여한 Amphotericin B와 Itraconazole의 효과와 이상 반응 비교

이상윤,박종선,김선영,양금진,박경덕,김학기,Lee, Sang-Yun,Park, Jong-Sun,Kim, Sun-Young,Yang, Keum-Jin,Park, Kyung-Deok,Kim, Hack-Ki 대한소아감염학회 2005 Pediatric Infection and Vaccine Vol.12 No.1

목 적 : 혈액종양 환아의 항암요법 후 발생한 호중구감소증 상태에서, 진균 감염은 높은 치명률을 가지는 것으로 알려져 있다. 진균 감염에 대한 경험적 항진균제로 주로 사용되는 ABV는 염증성 사이토카인인 IL-$1{\beta}$, TNF-${\alpha}$의 증가에 의해 발생하는 것으로 알려져 있는 발열, 오한, 발진, 신독성과 같은 부작용이 있다. Azole 계열의 ITZA도 광범위한 항진균 효과를 나타내고 있어 경험적 항진균제로의 사용이 고려되고 있는데 본 연구는 ABV와 ITZA의 정맥 주입에 따른 부작용의 발생 및 효능과 염증성 사이토카인 및 항염증성 사이토카인의 변화를 관찰하고자 한다. 방 법: 2004년 3월부터 2005년 2월까지 호중구감소증 상태에서 발열이 있어 치료한 급성 백혈병 환자를 대상으로 하였다. 대상으로 선정된 환자는 30명으로 ABV, ITZA 각각의 치료군은 15명이었다. 항진균제는 총 14일간 투여하였으며, 투여 후 혈청에 포함된 염증성 사이토카인(IL-$1{\beta}$, TNF-${\alpha}$)과 항염증성 사이토카인(IL-1Ra, IL-4)을 ELIZA를 통하여 측정하고, 치료 종료 시 치료 효과를 평가하였다. 결 과 : 두 치료군의 성별, 나이, 진단명, 항암치료의 단계, 마지막 항암요법의 시기 특성은 유의한 차이가 없었다. ABV 치료군에 비해 ITZA 치료군에서 정맥 주입 시 발생하는 이상 반응의 빈도가 적었다. 또한, ABV 치료군에서 ITZA 치료군에 비해 염증성 사이토카인인 IL-$1{\beta}$가 정맥주입 시 증가함을 보였고, IL-1Ra/IL-$1{\beta}$는 ABV 치료군에서는 감소하는 반면 ITZA 치료군에서는 증가함을 보였다. 결 론: 급성백혈병 소아에서 발열을 동반한 호중구감소증시 경험적 항진균제로 ABV와 ITZA를 사용하여 최종 치료 효과의 유의한 차이는 없었으나 정맥 투여와 연관된 이상 반응은 ABV 군에서 많았으며 호중구의 회복은 ITZA 군에서 빠른 것을 알 수 있었다. 이는 ABV나 ITZA 투여 시 시간에 따른 IL-Ra/IL-$1{\beta}$의 변화와 연관이 있을 것으로 생각된다. Purpose : Fungal infection is one of the important causes of morbidity and mortality in patients with hematologic malignancies. Amphotericin B(ABV) and itraconazole(ITZA) have been used as the standard empirical antifungal therapy in neutropenic patients with acute leukemia who have persistent fever that does not respond to antibiotic therapy. ABV is an antifungal drug associated with side effects such as fever and chills, symptoms which may be mediated by pro-inflammatory cytokines such as interleukin-$1{\beta}$(IL-$1{\beta}$) and tumor necrosis factor-${\alpha}$(TNF-${\alpha}$). We assessed modulation of these pro-inflammatory cytokines as well as the anti-inflammatory cytokines(IL-4, IL-1Ra) by ABV and ITZA. Methods : From March 2004 to February 2005, a total of 30 episodes from acute leukemia patients with febrile neutropenia were analyzed for this study. They were randomly allocated to receive intravenous ABV or ITZA for 14 days. Clinical responses were evaluated at the completion of therapy, and cytokine IL-$1{\beta}$, TNF-${\alpha}$, IL-4, and IL-1Ra were measured for determination to know the correlation between two antifungal agents and inflammatory cytokines. Results : Empirical antifungal agents were given to 37 patients(ABV 20, ITZA 17), and 30 patients(ABV 15, ITZA 15) were evaluable for efficacy. White blood cell and absolute neutrophil count in the group treated with ITZA increased early days of treatment, so the duration of neutropenia in ITZA group is shorter. Serum creatinine level is lower in ITZA group than in ABV group but this is not statistically significant. There was no significant difference in response rate between two groups. The IL-$1{\beta}$ was increased in ABV treatment group and the ratio of IL-1Ra/IL-$1{\beta}$ is markedly decreased in ABV treatment group while increased in ITZA group. Conclusion : ITZA and ABV have at least equivalent efficacy as empirical antifungal therapy in neutropenic children with acute leukemia. However ITZA is associated with significantly less toxicity in clinical and molecular aspects.

아토피피부염 및 건선 환자 증례를 통한 상한론 백호탕 조문의 ‘면구(面垢)’에 대한 새로운 해석

홍지은,박민철,강수진,양금진,조은희 한의병리학회 2020 동의생리병리학회지 Vol.34 No.1

This paper suggested MyeonGu(面垢), symptom of Baekho-tang(白虎湯) in text 219 of Shang-Han-Lun(傷寒論), could be reinterpreted. There were 4 cases, 1 case on atopic dermatitis and 3 cases on psoriasis. All of them were diagnosed by Ying-Ming-Bing(陽明病) symptom. After taking Baekho-tang, SCORAD index of atopic dermatitis in case 1 improved 62.8 to 12.8 and PASI score of psoriasis in case 2, 3, and 4 improved 13.0 to 2.4, 10.8 to 0.8, and 8.6 to 1.0 respectively. Since Baekho-tang was effective for certain skin disease with Ying-Ming-Bing(陽明病) symptom, MyeonGu could be related on skin problem. MyeonGu was mainly translated in ‘someone's face seems to be covered with dirt’. However if Myeon(面) is translated in ‘surface of the body’ and Gu(垢) is translated in ‘inflammation’, it means the surface of the body suffered from papules, erythema, white scales, etc. These are features of chronic inflammatory cutaneous disease like atopic dermatitis and psoriasis. This study is meaningful in case of giving specific clinical case of MyeonGu of Baekhotang text of Shanghanlun.

Expression profiling of microRNAs in lipopolysaccharideinduced acute lung injury after hypothermia treatment

이운정,김인수,신소영,박기철,양금진,은정우,설해정,정시경 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.3

We investigated the expression profiles of miRNAs in acute lung injury (ALI) rats after hypothermia treatment. ALI rats were induced with lipopolysaccharide (LPS) and maintained with hypothermia (HT) or normothermia (NT) for 6 hours. HT attenuated inflammatory cell infiltration in the lung and improved biochemical indicators of multi-organ dysfunction. Nineteen miRNAs were significantly differentially expressed in the HT group compared with the NT group. miR-142, miR-98, miR-541, miR-503, miR-653, miR- 223, miR-323 and miR-196b exhibited opposite patterns of expression between the two groups. These dysregulated miRNAs were mainly involved in the immune and inflammatory response on functional annotation analyses. This study shows that HT has lung protective effects and influences expression profiles of miRNAs in ALI. And dysregulated miRNAs after HT modulate the immune and inflammation in ALI. These results suggest that dysregulated miRNAs play a role in the mechanism of the lung protective effects of HT in ALI.