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안희진,이유미,박영석,장미숙,이승표,Ahn, Hee-Jin,Lee, Yoo-Mee,Park, Young-Seok,Chang, Mi-Sook,Lee, Seung-Pyo 대한영상치의학회 2010 Imaging Science in Dentistry Vol.40 No.3
Purpose : This study was to investigate whether cervical vertebrae can be utilized in evaluating the growth of the maxilla. Materials and Methods : Fifty one male patients took lateral cephalometric radiographs once in every two years from the age of 8 till 14. Measured parameters were the concavity depth at the lower border of the third and fourth cervical vertebrae, and three analytical maxillary dimensions. Results : The analysis of the maxillary measurements and the concavity depth at the lower border of the cervical vertebrae in the lateral cephalometric radiographs showed that the measured parameters gradually increased as the patients aged. Moreover, while the parameters of the patients in age 8 and 10 did not show any correlation, those of the patients in age 12 and 14 definitely showed the correlations. Conclusions : Although certain correlations were seen in particular parameters, further researches and studies with various parameters and shorter age intervals are needed. The result of this study will help clinicians in making plans and evaluating the proposed treatment plans.
생쥐 난자의 체외 성숙에 미치는 Melatonin의 영향
안희진,배인하,Ahn, Hee-Jin,Bae, In-Ha 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.3
Objective: Melatonin, which is secreted by pineal gland play an important role in the regulation of ovarian function via seasonal rhythm and sleep in most mammals. It also has a role in the protection of cells by removing toxic oxygen free radicals brought about by metabolism. In the present study, effects of melatonin on the mouse oocyte maturation were examined using two different culture conditions provided with 5% or 21% oxygen concentration. Material and Method: Immature mouse oocytes were obtained from the ovarian follicles of $3{\sim}4$ weeks old ICR strain mice intraperitoneally injected with 5 I.U. PMSG 44 hour before. Under stereomicroscope, morphologically healthy oocytes with distinct germinal vesicle (GV) were liberated from the graafian follicles and collected using mouth-controlled micropipette. They were then cultured for 17 hour at $37^{circ}C$, 5% $CO_2$ and 21% $O_2$ (95% air) or 5% $CO_2$, 5% $O_2$ and 90% $N_2$. New modified Hank's balanced salt solution (New MHBS) was used as a culture medium throughout the experiments. Effects of melatonin were examined at a concentration of $0.0001{\mu}M$, $0.01{\mu}M$ or $1.0{\mu}M$. For the prevention of spontaneous maturation of immature oocytes during culture, dibutyryl cyclic AMP (dbcAMP) and/or hypoxanthine were included in the medium. Results: Under 21% oxygen condition, oocytes cultured in the presence of $0.01{\mu}M$ melatonin showed a significantly higher maturation rates, in terms of germinal vesicle breakdown (95.0% vs 89.0%) and polar body formation (88.1% vs 75.4%), compared to those cultured with $0.0001{\mu}M$ or $1.0{\mu}M$ melatonin. However, no difference was observed in oocytes cultured under 5% oxygen whether they were treated with melatonin or not. In the presence of $0.01{\mu}M$ melatonin, oocytes either cultured under 21% or 5% oxygen exhibited no difference in the polar body formation (85.6% vs 86.7%). However, in the absence of melatonin, oocytes cultured under 21% oxygen exhibited lower polar body formation (74.7%). When oocytes were cultured in the presence of dbcAMP alone or with varying concentrations of melatonin, those treated with both compounds always showed better maturation, i.e., germinal vesicle breakdown and polar body formation, compared to those cultured with dbcAMP alone. At the same concentration of melatonin, however, oocytes exposed to 21% oxygen showed poor maturation than those to 5% oxygen. Similar results were obtained from the experiments using hypoxanthine instead of dbcAMP. Conclusion: Based upon these results, it is suggested that melatonin could enhance the meiotic maturation of mouse oocytes under 21% oxygen concentration, and release oocytes from the meiotic arrest by dbcAMP or hypoxanthine regardless of the concentration of oxygen, probably via the removal of oxygen free radicals.
미국 드라마를 활용한 지도가 대학생들의 영어듣기 능력 향상에 미치는 효과
안희진(Ahn Hee Jin),정동빈(Jeong Dong Bin) 영상영어교육학회 2011 영상영어교육 (STEM journal) Vol.12 No.1
The purpose of this study was to examine the effects of American soap operas on college students' listening ability. This research was conducted over a 12-week period with college students: two classes consisting of 27 and 26 students each. The classes were divided into an experimental group, which studied English through American soap operas, and a control group, which studied English using a typical listening text. Fifty three students in two classes were surveyed to investigate their previous learning experience and backgrounds. They were asked to take a pre-listening test to evaluate their listening proficiency. Their progress was assessed on the basis of a post-listening test. Finally, twenty seven students in the experimental group were administered to fill out a post-questionnaire in order to measure their perceptions and attitudes toward their learning experience. The findings of this study indicated that there was a statistically significant difference between the control group and the experimental group with respect to their post-test scores. However, there was no statistically significant difference not only between the highly proficient students in two groups but also between the students of low proficiency in two groups. The results of this study were discussed and pedagogical implication were suggested.
음식점 원산지 표시제 시행에 따른 소비자의 인식 및 요구도 조사 -서울시 거주하는 성인 대상-
안희진 ( Hee Jin Ahn ),박상현 ( Sang Hyun Park ),주나미 ( Na Mi Joo ) 대한영양사협회 2010 대한영양사협회 학술지 Vol.16 No.3
The purpose of this study was to investigate consumer awareness and demand related to country-of-origin labeling at restaurants, and to provide basic data to reexamine the need for current policies and to determine problems. The study found that 70% of the respondents thought that the implemented representation policy had improved food quality, and 81.3% of the respondents checked country-of-origin labeling at restaurants. In addition, 74.7% of the respondents answered that "reward for accusation" was appropriate policy. Regarding the degree of recognition of the meat importers, the respondents were well aware of the importing countries, but did not recognize the importing country of chicken. In terms of preference for meat importers, Australian beef was rated highest, but beef from the U.S. was ranked seventh. However, in preferences for pork and chicken, U.S. products were rated highest. According to the survey, in a question regarding the perception toward country-of-origin labeling, the respondents recognized that rice, beef, pork, and chicken were the targeted items. In addition, the respondents suggested that other food ingredients at restaurants should be designated as target items for country-of-origin labeling.
재외문화원의 프로그램 및 브랜드스케이핑 특성요소 분석에 관한 연구
안희진(Ahn, Hee-Jin),공순구(Kong, Soon-Ku) 한국실내디자인학회 2015 한국실내디자인학회 학술대회논문집 Vol.2015 No.10
Oversea Korean cultural centers play such an important role in improving national images, and as a public institution representing Korea, they are the nearest Korea to citizens in other countries of residence and really important organizations diffusing Korean culture and the Korean language at the same time. To improve national images and introduce the excellence of Korean culture around the world, therefore, it is necessary to analyze the roles and problems of oversea Korean cultural centers and develop various programs, further actively carrying out research on concrete directions for the interior design of oversea Korean cultural centers. Thus, this study aims to investigate the functions and roles of cultural centers in different countries and propose a direction for the interior design of oversea Korean cultural centers by analyzing their programs and interior design characteristics with brandscaping characteristic elements grafted so that oversea Korean cultural enters may fulfill their functions as a cultural promotion space in the process of interior design, which will increase in the near future. Based on the analysis results on the programs of oversea Korean cultural centers and brandscaping characteristic elements, oversea Korean cultural centers should adjust the rate of locally-diverse programs and draw up measures for spacial activation, while creating a new interior space to show differentiated national images according to the brandscaping characteristic elements.
김희선,설혜원,안희진,오선경,구승엽,김석현,최영민,김정구,문신용,Kim, Hee-Sun,Seol, Hye-Won,Ahn, Hee-Jin,Oh, Sun-Kyung,Ku, Seung-Yup,Kim, Seok-Hyun,Choi, Young-Min,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.4
Objective: This study was performed to evaluate the possibility of prolonged culture of human embryonic stem cells (hESC; SNUhES2) on human amniotic fluid cells (hAFC), which had been storaged after karyotyping. Method: The hAFC was prepared for feeder layer in the presence of Chang's medium and STO medium (90% DMEM, 10% FBS) at $37^{circ}C$ in a 5% $CO_2$ in air atmosphere. Prior to use as a feeder layer, hAFC was mitotically inactivated by mitomycin C. The hESCs on hAFC were passaged mechanically every seven days with ES culture medium (80% DMEM/F12, 20% SR, bFGF). Results: The hAFC feeder layer support the growth of undifferentiated state of SNUhES2 for at least 59 passages thus far. SNUhES2 colonies on hAFC feeder appeared slightly angular and flatter shape as compared with circular and thicker colonies observed with STO feeder layer and showed higher level with complete undifferentiation in seven days. Like hESC cultured on STO feeders, SNUhES2 grown on hAFC expressed normal karyotype, positive for alkaline phosphatase activity, high telomerase activity, Oct-4, SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 and formed embryoid bodies (EBs). Conclusion: The hAFC supports undifferentiated growth of hESC. Therefore, these results may help to provide a clinically practicable method for expansion of hESC for cell therapies.