Amoeba proteus 공생주의 질소 및 퓨린 대사에 있어서 생화학적 특이성의 분화

안태인,최의열 한국유전학회 1985 Genes & Genomics Vol.7 No.2

Diversification of the biochemical strain specificity between tD and symbiotic xD strain of A. proteus was studied by quantitative analysis of the intermediates, the end products, and the related enzymes in purine and nitrogen catabolism. Among the purine catabolites, the levels of allantoin in the two strains were the same. The amount of carbonyl diurea-crystals in xD strain was reduced to 60% compared with that of tD strain. The levels of ammonia and biuret reactant in xD cytoplasm were reduced to 46% and 21% of those in tD cytoplasm, respectively. The specific activities of glutamate dehydrogenase, glutamine synthetase, and aspartate transaminase did not differentiate the two strains. The levels of cytoplasmic free ammonia, biuret, and carbonyl diurea could be the possible markers for diversification of biochemical strain specificity of the two strains. The symbiotic bacteria in xD strain appeared to utilize the metabolic wastes of nitrogen and purine catabolism of the host.

1. 改造에 對 하야

安泰仁 대한전기학회 1948 전기의 세계 Vol.1 No.-

항생제 처리에 의한 공생박테리아의 Genetic System 및 그 산물의 역할 분석

안태인,안광석,이병은 한국유전학회 1986 Genes & Genomics Vol.8 No.4

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아메바 공생 세균의 groEx 에 의한 대장균의 열충격 보완과 열내성 증가

안태인,이정은,임쌍택 한국유전학회 2001 Genes & Genomics Vol.23 No.3

The groESLx operon cloned from endosymbiotic Gram-negative X-bacteria in the xD strain of Amoeba proteus has heat-shock consensus promoters at an extended region of 5' of the structural gene and specific promoters within the coding region of groESx. When the genes were subcloned in plasmid vectors, groESx and groELx could be highly expressed separately at temperatures above 30℃ by heat-shock consensus promoters and the specific promoters, respectively. E. coli groE mutants complementing these genes formed colonies at non-permissive temperatures for the mutants and supported plaque formation for λphage. Thus, the gene products were found to have chaperonin functions in heterologous combination with groE gene products of E. coli in vivo. In growth experiments, E. coli groE mutants transformed with groEx grew to a density twice that of E. coli groE mutants at 42 and 45℃. Accumulation of both GroESx and GroELx enabled the E. coli to maintain over 70% viability at 50℃ for 6hrs. Thus, accumulation of large amounts of GroESLx in E. coli appeared to promote cell growth and to confer thermotolerance at sublethal temperatures.

아메바의 공생세균에서 클로닝된 groEx 의 프로모터 영역에 결합하는 대장균 단백질의 탐지 및 특성에 대한 연구

안태인,이정은 한국유전학회 2000 Genes & Genomics Vol.22 No.2

The groEx gene cloned from symbiotic X-bacteria in Amoeba proteus contains specific strong promoters overexpressing without heat shock both in X-bacteria in symbiosis and in E. coli transformed with the gene, As X-bacteria are unable to grow in vitro, we detected and studied the characteristics of trans factors binding to the promoters in E. coli. Proteins were partially purified by ammonium sulfate precipitation and fractionation in heparin agarose column, and applied in the assay of gel retardation and Southwestern blotting of groEx promoters. We detected a 90-kDa protein specifically binding to the σ^(32)-dependent heat-shock consensus promoter (P1) of pgroExc. Proteins binding to σ^(70)-dependent promoters, P2 of pgroExc and P3 and P4 promoters of pgroELx DNA fragment were similar with those to lac promoter, though different in binding affinity. Thus, it is implied that E. coli contains a trans-factor protein acting specifically on P1 promoter of pgroExc and that trans factors binding to pgroELx DNA are the same as those to cis-elements for lac promoter.

생물교육 연구의 동향 : 영국의 Journal of Biological Education 의 컴퓨터 분석

안태인 한국과학교육학회 1989 한국과학교육학회지 Vol.9 No.1

For the advancement of research in biological education in Korea, research trends shown in Journal of Biological Education(JBE) of England were analyzed by using a PC program(REFMENU). Papers Published in JBE between 1977 and 1987 were registered on the program with classifying keys of biological education and biology including names of authors, year, title, volume pages, and key words. Those input-date were analyzed by sorting depend-ing on either the classifying keys or the key words. Among the 361 papers 28.8% was dealing with the theory of science education. The rest dealt with biology and biological education, together. Of the six categories of biological education, the research on biological curriculum was 41% of total and was the most. The major trends in this category was in developing the content of the curriculum. In the research of biological instruction, 37 papers dealt with the instruction theory and the rest 60 papers dealt with the tactics of instruction. Of the 60 papers on materials in biological education, the research in developing the biological material was the most. Thus, the general research trend was far more practical aspect than the theoretical aspect of biological education. In the analysis of the papers depending on the biological categories, the one dealt with ecology was the most(26.8%). The rest papers showed almost even distribution in the 13 categories of biology. The results of this analysis was discussed by comparing with the research trends in Korea to suggest the possible future studies.

Amoeba proteus 공생주 xD strain 에 있어서 박테리아 유전 기구의 역할 : 항생제를 이용한 분석 an Analysis Using Antibiotics

안태인,안광석 한국유전학회 1989 Genes & Genomics Vol.11 No.2

Roles of the bacterial genetic components in the endocellular symbiosis of xD strain of A. proteus were determined by application of plasmid curing agents and antibiotics. The effects of plasmid curing or blocking prokaryotic gene expression were analysed by two dimensional gel electrophoresis, autoradiography and experimental infection. Curing plasmids of the bacteria resulted in loss of the ability to infect the new host. By selective suppression of the transcription or translation of the symbiotic amoebae it was possible to localize the gene of xD strain specific protein in the bacterial chromosome. When the gene expression of bacterial chromosome was blocked, the symbiotic vesicles underwent degradation in xD strain and the bacteria were unable to develop the symbiotic vesicles in the newly infected host. Thus the development and maintenance of the symbiotic vesicle are under the control of the bacterial genome. Plasmids of the symbionts were found to be essential for the initial step of symbiosis by enabling the bacteria to avoid digestion in phagolysosomes.

아메바 세포막에 대한 단항체 생산 및 이를 이용한 막 조성 물질의 역할규명

안태인,최지영 한국통합생물학회 1989 동물학회지 Vol.32 No.4

Monoclonal antibodies (MAbs) reacting with the plasmalemma of Amoeba proteus were produced. Specificity of the 3 MAbs was determined by transfer blotting of the SDS polvacryfamide gel. AMS antibody reacted with the mucopolysaccharide bands of the spacer gel, 220 KD and 50 KD proteins of the resolving gel. The maior glycoprotein bands (175 KD, 165 KD) and 50 KD protein of the plasmalemma were recognized by AUG antibody. A third, AMP antibody reacted with the 50 KD protein only. In immunofluorescence microscopy of the enzyme treated cells, the antigens of these MAbs were sensitive to proteases, but not sensitive to neuraminidase. In the assay of cell to substratum attachment after binding with the antibody, AMG and AMP antibodies exerted no effect, but AMS hindered the attachment and cell spreading. Thus the effective components of the plasmalemma in cell to substratum attachment appear to be the mucopolysaccharides and 220 KD protein. The membranes of latex particle infested phagosomes did not show any distinction from the plasmalemma in fluorescence microscopy. Phagosome membranes of amoebae appear to be derived from the plasma membrane without selection in terms of the antigen composition. Amoeba Proteus의 세포막과 반응하는 단세포군 항체를 생산하였다. SDS polyacrylamide gel을 transfer blotting하여 이들 항체의 반응 특이성을 조사해 본 결과 AMS 단항체는 PAS로 염색되는 spacer gel의 mucopolysaccharide 린드, resolving gel의 220 KD 및 50 KD 단백질과 반응하였으며, 세포막의 주요 당단백질인 175 KD 및 165 KD 빈드와 50 KD 단백질은 AMG 단항체에 의해서 인지되었다. 그리고 AMP단항체는 공통인 50 KD 단백질과 특이하게 반응하였다. 효소처리한 아메바의 면역형광칠미경적 조사에서 이들 항체에 대한 항원분자들은 모두 단백질분해효소에 민감하였으며 neuraminidase에 대해서는 변화가 없었다. 이들 항체를 결합시킨 아메바의 용기표면 부착 가능성을 분석한 결과 AMP 및 AMG 단항체는 아무런 영향을 미치지 못하였으며 AMS 단항체는 세포의 용기표면 부착 및 세포의 펴짐을 저해하였다. 따라서 아메바의 용기표면 부착은 mucopolysaccharide 및 220 KD 단백질에 의해서 매게되는 것으로 나타났다. 그리고 latex particle을 담고 있는 식포막은 면역형광형미경적 조사에서 세포막과 차이가 없었다. 따라서 겐포막은 항원 조성에 있어서 비 선택적으로 세포막에서 유도되는 것으로 나타났다.

개선에 관하여

안태인 대한전기학회 1948 電氣工學 Vol.1 No.2

개선에 관하여

안태인 대한전기학회 1956 전기의 세계 Vol.1 No.2