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부분예혼합 저NOx Dual 버너가 설치된 수관식 보일러 연소실 수치해석
안준 한국연소학회 2018 한국연소학회지 Vol.23 No.4
A series of numerical simulations have been conducted for a combustion chamber of a water tube type boiler with dual partial-premixed burners. The burner was expected to give shorter flame to make the boiler be more compact. Two burners were predicted not to interact strongly so as to yield two semi-independent flames. 50% partial load operations were tried with 3 modes and the mode with lower burner was predicted to transfer heat to the combustion chamber most effectively.
안준,문준철,김주희,김금솔,장철성 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.-
Berry fruits have attracted attention because of their purported benefits for aging, cardiovascular disease, and cancer. Therefore, highly priced berry fruits might be targets for food adulteration and fraud. In this study, eight speciesspecific primer sets based on the single nucleotide polymorphism of the chloroplast genomes of four berry fruits (aronia, blackberry, cranberry, and strawberry) were developed for quantitative real-time PCR (qPCR) analysis by SYBR Green staining with the aim of preventing berry fruit food fraud. The developed primer pairs exhibited high efficiencies ranging from 88 to 110% with strong correlation coefficients ( R2 > 0.99) for the amplification of each target species. However, no clear correlation coefficients were evident for non-target species. To evaluate the practicality of the developed primers, 18 commercial berry fruit products were verified by qPCR analysis. The developed primer pairs were amplified to a low Ct value (range 16.1–23.3) for the target species and proved capable of detecting target species in berry fruit commercial foods. Therefore, the developed qPCR-based species-specific markers could be suitable for the prevention of berry fruit food fraud and to verify marker reliability.
안준,문준철,장철성 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.5
Human consumption of plant functional foods has been rapidly increasing owing to the health benefits they provide. In particular, in Korea, the plant Orostachys japonica has attracted attention for its anticancer and other effects. Of the 12 established Orostachys species, only three (viz., O. iwarenge, O. malacophyllus, and O. japonica) have been allowed for use as foods in Korea. In this study, 12 species-specific primer sets based on single nucleotide polymorphisms of five chloroplast genes and one nuclear gene were developed to discriminate Orostachys species through quantitative real-time PCR (qPCR) analysis with SYBR Green staining. The efficiencies of the designed primer pairs in amplifying the target species ranged from 80 to 110%, with strong correlation coefficients (R2[0.99), whereas no clear correlation coefficient was evident for the non-target species. In order to verify the specificity of the 12 developed Orostachys-specific primers, binary mixtures of the DNAs (tenfold serially diluted samples) from the target species and each of the other non-target species were generated for qPCR analysis, with results suggesting that the primers could clearly discriminate at least 0.1% of O. japonica DNA (10 pg) in the mixtures. With regard to the feasibility of the developed qPCR system for detecting Orostachys species in O. japonica food products, O. japonica DNA was detected in all eight commercial products tested, with low Ct values (\20), whereas none of the other Orostachys species DNAs were detected, confirming that the tested foods contained only O. japonica. Therefore, developed primers and qPCR conditions would be useful for verifying the authenticity of commercial O. japonica food products.
Combustion and heat transfer characteristics inside the combustion chamber of a wood pellet boiler
안준,김종진 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.2
A grate-firing boiler was developed for wood pellet fuel, and then its combustion characteristics were tested. The flame was stretchedto the exit of the combustion chamber, implying insufficient space for complete combustion. As a first step to resolve this problem, anumerical simulation was conducted for the combustion chamber. Turbulent and chemically reacting flow was considered by implementinga homogeneous reaction model. Flow field from the simulation showed strong recirculation flow at the upstream corner of the chamber,along which the flame was stretched to the exit. Based on these results, we suggest possible modification of the combustion chamberto improve combustion characteristics, such as relocating its exit or installing internals like guide vanes.
Oxy-fuel combustion boiler for CO2 capturing: 50 kW-class model test and numerical simulation
안준,김혁주,최규성 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.10
A novel oxy-fuel burner was devised and integrated into a 50 kW-class furnace-type boiler system. A series of experiments was conducted to verify its feasibility for industrial applications. Additionally, numerical simulations were performed and the results validated against experimental data on the detailed physics inside the conventional-design combustion chamber. The oxy-fuel burner, with the help of gas radiation, could effectively heat the combustion chamber. The composition of the exhaust gas revealed that the sealing of the system is crucial to the achievement of high CO2 concentrations and low NOx emissions.