순채 (蓴采) ( Brasenia purpurea ) 자생 저수지인 (自生貯水池) 금호제와 척포제의 육수학적 (陸水學的) 연구

안영근,주흥규,서화중 ( Young Keun Ahn,Heung Kyu Joo,Hwa Jung Sheo ) 한국하천호수학회 1977 생태와 환경 Vol.10 No.3·4

The purpose of this study is to elucidate the characteristics of Brasenia purpurea wild growing reservoirs(Keum Ho and Cheok Po Jae) by comparing the limnological condition with other reservoir(Kim Si Jae) which has no wild growing of this plant of Chun Nam Province during Spring, Summer, and Autumn in 1977. The results obtained are as follows 1. B. purpurea wild growing reservoirs. Cotaining small reservoirs in Keum Ho Jae catchment and the greater part of the woody land in Cheok Po Jae catchment all diminish the earth carrying into the reservoirs and keep up the bottom of the basin structure formed spontaneously by earth dam erosin for long time. On account of the bottom basin a little water are left after maximum drainage and aquatic plants are protected from drying up. Therefore thick nutrious sediment layer are formed in the bottom by plant deposit. Low water depth at all times, making latent capacity in store large in flood and flushing rate small, diminish the loss of nutrients from the reservoirs. Above all condition with the comparatively large loading rate of nutrients make the reservoirs high eutrophication. The high concentration of P, K, N, Mg, Ca, optimum temperature, and high transparence elevate highly the photosynthetic faculty of plant. The highly saturated DO elevate the oxidative autopurification faculty and accelerate the inorganization of organic substance and accumulate a lot of nutrients in the reservoirs by increasing the precipitation of the insoluble phosphate and suppressing the denitrification in the bottom water layer. The high concentration of the total salt seem to prevent dissolving the gel which cover the young leaf of B. purpurea. Therefore B. purpurea wild growing reservoirs are very likely to have symmetrical environment and fit for the aquatic plant. 2. B. purpurea non-growing reservoir On account of the major part of the bare land in the catchment the large amount of the earth are carried into the reservoir in flood, having neither basin structure nor sediment in the bottom. Small latent capacity in store make flushing rate large in flood and the big drainage rate make the loss of nutrients large. Above all condition with the comparatively little loading rate of nutrients result the reservoir in poor nutrient accumulation. The low concentration of nutrient salt, deep water, and high turbidity make unfit environment for aquatic plant growing, especially for B. purpurea.

Amygdalin의 경구투여독성에 미치는 Cysteine과 Streptomycin의 영향

안영근,오종,정지건,김정훈,Ahn, Young-Keun,Oh, Chong,Chung, Zi-Gun,Kim, Jong-Hoon 환경독성보건학회 1989 환경독성보건학회지 Vol.4 No.3

Amygdalin has been used for a long time as an anticancer agent. But because of its toxicity, it is difficult to administer continuously for treatment of cancer. This paper was attempted to reduce the side effect and toxicity of amygdalin. That is, effects of cysteine and streptomycin on the toxicity of amygdalin were investigated in rats orally administered amygdalin. 1. The group administered only amygdalin 400 mg/kg was effected on the lung and body weight, hematocrit, hemoglobin, clotting time, SGOT and albumin value. That is, lung and body weight, hematocrit hemoglobin and albumin value were significantly decreased. SGOT and clotting time were significantly increased compared with those of normal group. 2. Weight of lung was significantly increased in the C group (administred amygdalin 400 mg/kg and cysteine 300 mg/kg), D group (amygdalin 400 mg/kg and streptomycin 10 mg/kg), E group (amygdalin 400 mg/kg, streptomycin 10 mg/kg and cysteine 200 mg/kg)and F group (amygdalin 400 mg/kg, streptomycin 10 mg/kg and cysteine 300 mg/kg). 3. Values of hematocrit and hemoglobin were significantly increased, and clotting time was significantly decreased, in the I group and F group compared with those of A group. 4. SGOT was significantly decreased in the C group, E group and F group compared with that of A group. 5. The blood cyanide concentration was significantly decreased in the E group and F group compared with that fo A group. 6. In short, coadministration of cysteine and streptomycin are considered to reduce the toxicity of amygdalin in rats orally administered.

마우스에 있어서 에이코사펜타엔산이 면역반응에 미치는 영향(I) I. 체액성 면역

안영근(Young Keun Ahn),김정훈(Joung Hoon Kim),이상근(Sang Keun Lee),김행순(Haeng Soon Kim) 대한약학회 1989 약학회지 Vol.33 No.1

The humoral immune response of Eicosapentanoic acid(EPA) was investigated in mice. ICR male mice were divided into 8 groups and received intraperitoneal injection of EPA(5mg, 10mg, 20mg/kg) for 4 weeks. Cyclophosphamide(5mg/kg) was administered i.p. 2 days prior to secondary immunization. Humoral immune response was evaluated by antibody titer, hypersensitivity to SRBC(Arthus), plaque forming cell(PFC) and organ weight. The obtained results were as followings: The increased rate of body weight, the ratio of liver weight, spleen weight to body weight were decreased by all EPA administration groups as compared to normal group. HA titer, HY titer and Arthus reaction were enhanced according to the increase of EPA doses as compared to normal group. PFC was significantly enhanced by EPA 10mg administration group. These results suggest that EPA enhances humoral immune response to SRBC in mice, indicating that EPA may block a immunoglobulin synthesis inhibition of arachidonic acid.

베타-카로틴의 면역생물학적 연구

안영근(Young Keun Ahn),구자돈(Ja Don Koo),김정훈(Joung Hoon Kim),김봉희(Bong Hee Kim),조필형(Phil Hyoung Cho),구교임(Kyo Im Koo) 대한약학회 1992 약학회지 Vol.36 No.5

Effects of beta-carotene on the immunobiological responses were studied in ICR mice. ICR male mice were divided into 8 groups (10 mice/group), and beta-carotene at doses of 4, 20 and 100mg/kg were orally administered to ICR mice once daily for 28 consecutive days. Cyclophosphamide (CY) was injected intraperitoneally (i.p.) to ICR mice with a single dose of 5mg/kg body weight at 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by humoral immunity, cellular immunity and non-specific immunity. The results of this study were summarized as follows; (1) Beta-carotene significantly increased the weight ratios of liver, spleen and thymus to body weight depending on dose, and significantly increased the increasing rate of body weight and the number of circulating leukocyte. (2) Beta-carotene dose-dependently increased hemagglutination titer, Arthus reaction and hemolytic plaque forming cell related to humoral immunity. (3) Beta-carotene significantly increased delayed-type hypersensitivity reaction and rosette forming cell related to cellular immunity. (4) Beta-carotene dose-dependently increased phagocytic activity, and significantly increased natural killer (NK) cell activity. (5) Beta-carotene dose-dependently inhibited reductions in humoral immunity, cellular immunity, NK cell activity and phagocytic activity by treatment with CY.

염화아연이 생쥐의 면역반응에 미치는 영향

안영근(Young Keun Ahn),김정훈(Joung Hoon Kim),채병숙(Byung Sook Chae),차광재(Kwang Jae Cha) 대한약학회 1992 약학회지 Vol.36 No.4

Effects of Zinc chloride on the immune responses were studied in ICR mice. ICR male mice were divided into 5 groups(10 mice/group) and Zinc chloride at doses of 0.3, 1.2, 4.8 and 19.2mg/kg were orally administered to ICR male mice once a day for three weeks. Mice were sensitized and challenged with sheep red blood cells(S-RBC). The results of this study were summarized as follows; (1) Zinc chloride significantly increased the body weight rate, the weight ratios of spleen and thymus to body weight and the number of circulating leukocyte, but significantly decreased them at the high dose of it, and increased dose-dependently the weight ratio of liver to body weight. (2) Zinc chloride significantly increased hemagglutination titer, Arthus reaction and plaque forming cell related to humoral immunity, but significantly decreased them at the high dose of it. (3) Zinc chloride significantly increased delayed type hypersensitivity reaction and rosette forming cell related to cellular immunity, but significantly decreased them at the high dose of it. (4) Zinc choride significantly enhanced phagocytic activity, but significantly decreased according to the increase of its dose. These results suggest that high dose of zinc chloride decreased humoral, cellular and non-specific immune responses.

Quercetin이 마우스의 면역반응에 미치는 영향

안영근(Young Keun Ahn),박영길(Young Gil Park),김정훈(Joung Hoon Kim) 대한약학회 1991 약학회지 Vol.35 No.5

Effects of quercetin on the specific and non-specific immune responses were studied in vivo. Quercetin at a dose of 2.5, 5, 10, 20 and 40mg/kg were orally administered to ICR male mice once daily for 28 consecutive days. Cyclophosphamide was injected intraperitoneally to ICR mice with a single dose of 5mg/kg 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by humoral and cellular immune reponses and non-specific immune response. The results of this study were summarized as followings; 1. Quercetin significantly decreased the body weight, and introduced the atrophy of liver, spleen and thymus gland dose-dependently, but increased the numbers of white blood cell. 2. Querectin significantly depressed the hemagglutination titer, Arthus reaction and hemolytic plaque forming cell. 3. Quercetin significantly depressed the delayed type hypersensitivity and rosette forming cell. 4. Quercetin at a dose of 2.5, 5 and 40 mg/kg significantly depressed phagocytic activity. 5. Quercetin at a dose of 10 and 20 mg/kg significantly increased natural killer cell activity.

마우스에 있어서 에이코사펜타엔산이 면역반응에 미치는 영향(II) II. 세포성 면역 및 비특이적 면역

안영근(Young Keun Ahn),김정훈(Joung Hoon Kim),이상근(Sang Keun Lee),김행순(Haeng Soon Kim) 대한약학회 1989 약학회지 Vol.33 No.1

The cellular and nonspecific immune response of EPA were investigated in mice. ICR male mice were divided into 8 groups and received intraperiteneal injection of EPA(5mg, 10mg, 20mg/kg) for 4 weeks. Cyclophosphamide(5mg/kg) was administered i.p. 2 days prior to secondary immunization. Immune responses were evaluated by hypersensitivity to SRBC(DTH), rosette forming cell(RFC), natural killer(NK) cell activity and macrophage activity. The obtained results were as follows: As compared to normal group, 1) DTH was increased by EPA 5mg, 10mg administration groups. 2) RFC was significantly increased by EPA 20mg administration group. 3) NK-Cell activity was significantly increased by EPA 10mg administration group. 4) Macrophage activity was enhanced by EPA 5mg administration group.

생쥐에 있어서 에탄올의 면역독성에 미치는 메치오닌 식이의 영향

안영근(Young Keun Ahn),김정훈(Joung Hon Kim),구기범(Gi Bum Koo),문재규(Jae Gyu Moon) 대한약학회 1992 약학회지 Vol.36 No.4

Experiments were performed on mice to investigate the effect of methionine diets (MET) on the immunotoxicity of ethanol. ICR female mice were divided into 5 groups, Met (Basal (B) + O.19% methionine(M), B + 1.71% M and B + 5.13%(M) and ethanol(4%) were administered ad libitum for 21 days. The mice were evaluated for changes in immune status as measured by antibody titer, Arthus reaction, delayed type hypersensitivity (DTH), rosette forming cell(RFC) and plaque forming cell (PFC) to sheep red blood cells (S-RBC). To investigate the change of the non-specific immune response, the number of leukocytes in peripheral blood and phagocyte activity were measured. The results were summarized as follows: (1) The weight ratios of spleen and thymus to body weight were significantly increased by the B + 0.19% M, B + 0.57% M and B + 1.71% M groups in comparison with control group(B), but B + 5.13% M group was significantly decreased. (2) Humoral immune responses were significantly increased by the B + 0.19% M and B + 0.57% M groups in comparison with control group, but B + 5.13% M group was significantly decreased. (3) Cellular immune responses were significantly decreased by the B + 1.71% M and B + 5.13% M groups in comparison with control group. (4) Phagocyte activities were significantly increased by the B + 0.19% M, B + 0.57% M and B + 1.71% M groups in comparison with control groups, but B + 5.13% M group was significantly decreased. (5) The number of circulating leukocyte was significantly increased in the B + 0.19% M and B + 0.57% M groups in comparison with control group, but B + 5.13% M group was significantly decreased.

월견초종자유가 생쥐의 면역반응에 미치는 영향

안영근(Young Keun Ahn),오연준(Yun Joon Oh),김정훈(Joung Hoon Kim) 대한약학회 1992 약학회지 Vol.36 No.2

The purpose of this experiment was to investigate both the immunomodulatory effect of evening primrose(EP) oil and the effects of EP oil on immunoregulation by cyclophosphamide in mice. EP oil at doses of 0.1, 0.2 and 0.4ml/kg were orally administered to ICR male mice once daily for 28 consecutive days. Cyclophosphamide was injected intraperitoneally to ICR mice with a single dose of 5mg/kg at 2 days before secondary immunization . Mice were sensitized and cellular immune responses and non-specific immune response. The results of this study were summarized as follows; (1) The humoral immune responses such as hemagglutination titer(HA), hemolysin titer(HY)Arthus reaction and plaque forrming celll(PFC) were significantly enhanced in the low dose EP oil administered groups(0.1 and 0.2ml/kg). However, in the high dose EP oil administered group(0.4ml/kg) the responses were significantly lowered. (2) In the case of cellular immune responses, delayed type hypersensitivity reaction(DTH) was significantly decreased in EP oil whereas rosette forming cell(RFC)was remarkably enhanced. (3) Activities of natural killer cells and phagocyte were generally enhanced in EP oil. In addition, serum albumin and globulin were also increased.

관상동맥 중재술을 시술받은 불안정형 협심증 환자에서 저분자량 헤파린의 장기 효과

홍영준(Young Joon Hong),정명호(Myung Ho Jeong),이승현(Seung Hyun Lee),박옥영(Ok Young Park),김주한(Ju Han Kim),김원(Weon Kim),류제영(Jay Young Rhew),안영근(Young Keun Ahn),조정관(Jeong Gwan Cho),박종춘(Jong Chun Park),서순팔(Soon Pa 대한내과학회 2002 대한내과학회지 Vol.63 No.2

배경: 급성 관동맥 증후군 환자에서 저분자량 헤파린은 기존의 미분획 헤파린에 비해 aPTT monitoring이 필요 없으며 피하주사로 쉽게 투여할 수 있다는 장점과 함께 주요 심장사고를 감소시키는 것으로 알려져 있다. 목적: 불안정형 협심증으로 내원하여 관상동맥 중재술을 시행한 환자 중 Dalteparin (Fragmin)을 사용했던 군과 미분획 헤파린을 사용했던 군 사이에 사망, 급성 심근경색증, 표적 병변 재개통술, 재협착률 등의 주요 심장사고 및 합병증에 대해 알아보기 위하여 본 연구를 시행하였다. 대상 및 방법: 1999년 1월부터 2001년 6월까지 전남대학교병원 심장센터에 입원하여 관상동맥 중재술을 시행 받고 6개월 이후에 추적 관상동맥 조영술을 시행한 불안정형 협심증 환자 180예를 대상으로 Dalteparin (Fragmin)을 사용했던 90예의 환자군을 I군(61.8±8.9세, 남:61, 여:29)으로, 미분획 헤파린을 사용했던 90예의 환자군을 II군(62.6±9.7세, 남:63, 여:27)으로 각각 분류하고 양군 사이에 임상적 및 관상동맥 조영술 특성과 주요 심장사고, 합병증에 대해 비교하였다. 결과: 1) I군에서 당뇨병 환자가 많았던 것 이외에(I군; 38/90명, 42.2% vs II군; 27/90명, 30.0%, p=0.021) 양 군 사이에 임상 양상에 있어서 유의한 차이는 없었다. 2) 병변 혈관의 수, 경색관련 혈관의 분포, TIMI flow, ACC/AHA 형태, 혈전 내재 병변, 스텐트 사용 여부, 병변 길이 등 관상동맥 조영술상 양군간에 유의한 차이는 없었다. 3) 재원 기간 중 급성 심근경색증, 표적 혈관 재개통술, 사망 등 주요 심장 사고에 있어서 양군간에 차이는 없었다. 4) 6개월 추적 관상동맥 조영술상 I군에 비해 II군에서 최소 혈관 내경의 유의한 감소 및 내경 협착률에 있어서 유의한 증가를 보였다(1.81±0.49 mm vs 1.64±0.44 mm, 32.2±14.5% vs 37.4±18.8%, 각각 p=0.035, 0.041). 5) 6개월 이후 추적 관상동맥 조영술상 재협착률은 I군에서 유의하게 낮았고(I군 26/90명, 28.8% vs II군 32/90명, 35.6%, p=0.041), 표적 혈관 재개통술에 있어서 I군에서 유의하게 낮았으나(I군 21/90명, 23.3% vs II군 27/90명, 30.0%, p=0.039), 사망률에 있어서 양군 사이에 유의한 차이는 없었다. 6) 합병증에 있어서 심각한 출혈성, 경미한 출혈성 부작용 및 허혈성 뇌졸중, 혈소판 감소증에 있어서 양군 사이에 유의한 차이는 없었다. 7) 관상동맥 중재술 후 재협착에 관여하는 인자는 스텐트 사용 여부, 병변 길이, 관상동맥 중재술 후 최소 혈관 내경, 내원 당시 CRP 수치, 당뇨병 동반 여부, 사용하였던 헤파린의 종류였다(p=0.032, 0.001, 0.001, 0.011, 0.022, 표 6). 결론: 불안정형 협심증 환자에서 저분자량 헤파린인 Dalteparin (Fragmin)을 사용했던 환자군에서 미분획 헤파린을 사용했던 환자군에 비해서 재협착률 및 관상동맥 재개통술이 유의하게 낮았다. Background: Antithrombotic therapy with heparin reduces the rate of ischemic events in patients with acute coronary syndrome. Low-molecular-weight heparin (LMWH), given subcutaneously twice daily, has a more predictable anticoagulant effect than standard unfractionated heparin, is easier to administer and does not require monitoring. Methods: We prospectively analyzed 180 patients with unstable angina who underwent percutaneous coronary intervention (PCI) between 1999 and 2001 at Chonnam National University Hospital to receive either 120 U/kg of Dalteparin (Fragmin), administered subcutaneously twice daily (group I; n=90, 61.8±8.9 years, male 67.8%), or continuous intravenous unfractionated heparin (group II; n=90, 62.6±9.7 years, male 70.0%). During hospitalization and at 6 month after PCI, major adverse cardiac events such as acute myocardial infarction, target vessel revascularization, death, or restenosis were examined. Results: During hospitalization, the incidence of acute myocardial infarction, target vessel revascularization and death were not different between two groups. At follow-up coronary angiography at 6 month after PCI, the incidence of restenosis was lower in group I than in group II (Group I; 26/90, 28.8% vs. Group II; 32/90, 35.6%, p=0.041) and the incidence of target vessel revascularization was lower in group I than in group II (Group I; 21/90, 23.3% vs Group II; 27/90, 30.0%, p=0.039). There was no difference in the rate of major and minor hemorrhage, ischemic stroke and thrombocytopenia between two groups. In the multivariate analysis, factors relating to restenosis were lesion length, postprocedural minimal luminal diameter, CRP on admission, diabetes mellitus, type of hepairn, stent use. Conclusion: Dalteparin, a LMWH, is superior to standard unfractionated heparin for reducing restenosis rate and target vessel revascularization without increasing bleeding complications. (Korean J Med 63:158-168, 2002)