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미세유체채널 내에서 가해진 복합적 자극에 따른 미세아교세포의 활성도 및 운동성 변화 양상에 관한 연구
안송이(Song Ih Ahn),송석현(Sukhyun Song),신현준(Hyun Jun Shin),박진성(Jin-Sung Park),신현정(Jennifer H. Shin) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
In neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease, microglia, a type of brain cells, play critical role as immune cells depending on their activation states. Microglia undergo significant changes in their activation states, which determine their functional roles and migration patterns, upon various physicochemical stimulations from the microenvironment of the brain. Moreover, brain cells can be exposed to exogenously imposed physical stimulations from therapeutic treatments. Therefore, it is important to understand how physical cues influence the activation states of microglia. Our ability to regulate the activation state by physical stimulations can provide new insights for pathological outbreaks of such diseases and may lead us to a novel therapeutic tool. To investigate how physical cues influence the activation states of microglia, we developed a microfluidic platform that can apply the combinatorial stimulation of an fluid shear stress and electric field on microglial cells by controlling two directional cues independently.
안송이(Song Ih Ahn),홍정우(Jung Woo Hong),신현정(Jennifer H. Shin) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
Sphingosylphosphoryl choline (SPC) is a bioreactive lipid, present in abnormally high concentrations in pancreatic cancer patients. SPC is known to facilitate cancer metastasis by altering cytokeratin network, enhancing cell migration. In this study we investigated the effects of SPC on cell migration behavior, focusing on the major players in cell migration, actin cytoskeleton structure and cell traction force rather than cytokeratin network. From our preliminary tests of immunofluorescence analysis and traction force microscopy, treatment of SPC induced higher expression of lamellipodia and lower traction force, respectively. These results concur to the fact that SPC enhances cell migration, since motile cells are known to actively express lamellipodia and form weak bindings to the substrate. We suggest that SPC stimulation in pancreatic cancer cells would enhance cell migration by modulating cellular traction force and actin cytoskeletal network, which in turn facilitates metastasis.