남해안 통영지역 가두리양식장 해수-퇴적물 경계면에서의 chemical fluxes

심정희,강영철,최진우,Shim, Jeong-Hee,Kang, Young-Chul,Choi, Jin-Woo 한국해양학회 1997 바다 Vol.2 No.2

1995년 6월, 남해안 통영지역 가두리양식장 해수-퇴적물 경계면에서 입자상유기물의 수직유입량과 용존산소의 소모량, 영양염류의 용출량을 관측하였다. 입자상유기물의 수직유입량은 저층고정식 sediment trap을 이용하였으며, 용존산소의 소모량과 영양염류의 용출량은 benthic chamber method로 측정하였다. 가두리양식이 연안부영양화에 미치는 영향과 가두리에서 유출된 입자상유기물의 확산강도를 정량하기 위해, 가두리 아래(수심 약 18 m, Cage Site)와 가두리에서 수평으로 약 100 m 가량 떨어진 곳(수심 약 32 m, Control Site)의 해수-퇴적물 경계면에서, 암모니아와 인산염, 규산염의 용출량을 비교하고, 탄소와 질소와 인의 mass balances를 추정하였다. 관측결과, 가두리정점(Cage Site)으로는 6400 mg C $m^{-2}d^{-1}$의 입자상유기물이 유입되었고, 동시에 230 mmol $O_2\;m^{-2}d^{-1}$ 이상의 용존산소가 소모되었다. 따라서 탄소의 경우, 가두리 아래 해저면으로 공급되는 유기 입자의 약 40%에 달하는 양이 해수-퇴적물 경계면에서 분해되며 (ca. 2400 mg C $m^{-2}d^{-1}$), 나머지 약 60%는 퇴적되어 매몰되는 것으로 보인다. (ca. 4000 mg C $m^{-2}d^{-1}$) 그러나 대비정점(Control Site)에서는 가두리정점에 비해 상대적으로 적은 양의 유기물유입과(ca. 4000 mg C $m^{-2}d^{-1}$), 낮은 용존산소소모율이 관측되었다(75 mmol $O_2\;m^{-2}d^{-1}$). 관측결과는 가두리에서 투기되는 대부분의 입자상유기물이 가두리 아래 해저면에 집중적으로 퇴적되고 있음을 보여주며, 가두리 부근 해저면으로 확산되는 입자상유기물의 양은 가두리에서 멀어질수록 급속히 감소함을 시사한다. Benthic respiration and chemical fluxes were measured at the sediment-water interface underlying the marine fish cages floating on the open coastal waters off Tong-Young, the South Coast of Korea. The effects of cage farming on coastal benthic environment and on mass balance of organic carbon in the benthic boundary layer under the marine fish cages are addressed. In a growing season of caged fishes of June, 1995, benthic chambers and sediment traps were deployed on the sediment-water interfaces of the two sites chosen for this study: 1) Cage Site, directly underlying the fish cages of the farm at 18 m water depth, and 2) Control Site, about 100 m away from the farm at 32 m water depth. Benthic respiration rates and chemical fluxes were calculated from the evolution of dissolved oxygen and chemicals in the chamber water, and mass balance of organic carbon in the benthic boundary layer was constructed based on the vertical flux of particulate organic matter (POM) and chemical fluxes out of the sediment. High organic dumping (6400 mg C $m^{-2}d^{-1}$) and high benthic respiration (230 mmol $O_2\;m^{-2}d^{-1}$) were observed at the Cage Site. Equivalent to 40% of vertical flux of organic carbon into the Cage Site seemed to be decomposed concurrently and released back to overlying waters (2400 mg C $m^{-2}d^{-1}$). Consequently, up to 4000 mg C $m^{-2}d^{-1}$ of organic carbon could be buried into the farm sediment (equivalent to 60% of organic carbon flux into the Cage Site). At the Control Site, relatively less input of organic carbon (4000 mg C $m^{-2}d^{-1}$) and low benthic respiration rate (75 mmol $O_2\;m^{-2}d^{-1}$) were observed despite short distance away from the cages. The influence of cage farming on benthic chemical fluxes might be restricted and concentrated in the sea bottom just below the fish cages in spite of massive organic dumping and high current regime around the fish cage farm.

중년 여성의 체형에 대한 자기 평가

심정희 한국의류학회 2003 한국의류학회지 Vol.27 No.1

The purpose of this study is to provide total data of real body-type and self-evaluation about it. The subjects were 614 middle-aged women between 35 and 59 years old. Data were collected through measurement and a questionnaire survey on self-evaluation. The results of self-evaluation of body are as follows; 1. As the result of the recognition degree analysis, people consider themselves thicker, shorter or smaller than what they really are, regardless of ages and types. In addition, the women of younger group rather than the women of order group consider themselves bigger/thicker and shorter than what they really are. They think that the items of waist girth, abdominal girth, hip girth, upper arm girth, and thigh girth are thicker than the other items, however, among girth items the size of bust girth is smaller than the other items. The women of the late middle-aged group consider their somatotype thicker/bigger and shorter than the women of the early middle-aged group do. 2. As the result of body cathexis for each part, women in general turn out themselves not to be satisfied with their body parts, regardless of ages and types. Particularly, they are not satisfied with abdominal girth, weight, hip girth. According to the age bracket, the women in the older group are more satisfied than the women of younger group in terms of their body. According to somatotype, women in tall and slim groups are more satisfied with their body. The women of the early middle-aged group turn out not to be satisfied with girth items while the women of the late middle-aged group are not satisfied with length items.

Proteomic Analysis of Time-Dependant Difference of Protein Expression Profile Changes during Neuronal Differentiation of Mouse Embryonic Stem Cells

심정희,조선아,서민지,김정희,류나경,유경현,양문희,김세윤,박영일,박종훈 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

KCl Mediates K+ Channel-Activated Mitogen- Activated Protein Kinases Signaling in Wound Healing

심정희,임종우,김병규,박수진,김석화,최태현 대한성형외과학회 2015 Archives of Plastic Surgery Vol.42 No.1

Background Wound healing is an interaction of a complex signaling cascade of cellular events, including inflammation, proliferation, and maturation. K+ channels modulate the mitogen-activated protein kinase (MAPK) signaling pathway. Here, we investigated whether K+ channel-activated MAPK signaling directs collagen synthesis and angiogenesis in wound healing. Methods The human skin fibroblast HS27 cell line was used to examine cell viability and collagen synthesis after potassium chloride (KCl) treatment by Cell Counting Kit-8 (CCK-8) and western blotting. To investigate whether K+ ion channels function upstream of MAPK signaling, thus affecting collagen synthesis and angiogenesis, we examined alteration of MAPK expression after treatment with KCl (channel inhibitor), NS1619 (channel activator), or kinase inhibitors. To research the effect of KCl on angiogenesis, angiogenesis-related proteins such as thrombospondin 1 (TSP1), anti-angiogenic factor, basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), pro-angiogenic factor were assayed by western blot. Results The viability of HS27 cells was not affected by 25 mM KCl. Collagen synthesis increased dependent on time and concentration of KCl exposure. The phosphorylations of MAPK proteins such as extracellular-signal-regulated kinase (ERK) and p38 increased about 2.5–3 fold in the KCl treatment cells and were inhibited by treatment of NS1619. TSP1 expression increased by 100%, bFGF expression decreased by 40%, and there is no significant differences in the VEGF level by KCl treatment, TSP1 was inhibited by NS1619 or kinase inhibitors. Conclusions Our results suggest that KCl may function as a therapeutic agent for wound healing in the skin through MAPK signaling mediated by the K+ ion channel.

울진군 대형산불 발생 인근 마을어장의 해조류 군집 특성

심정희,최희찬,정해근,갈종구,심정민,홍석익,권철휘,김상우 해양환경안전학회 2023 해양환경안전학회지 Vol.29 No.2

본 연구에서는 울진군 대형산불 발생이 연안해역에 미치는 영향을 파악하기 위하여 울진군 나곡(F-1), 후정(F-2), 봉평(F-3), 공세항(F-C)에서 수질과 함께 해조류의 종조성, 우점종 및 군집 특성을 분기별로 조사하였다. 수질 분석 결과, 산불에 대한 영향을 판단하기 위한 수소이온농도(pH)는 표․저층에서 각각 8.07~8.30과 8.12~8.48 이었다. 본 연구의 pH 값은 동해의 연안 해수에 있는 일반적인 농도 범위에 포함된 값으로 산불로 인한 직접적인 영향으로 볼 수 없었다. 연안 조하대에서 조사한 해조류의 분석 결과, 전 시기에 대한 해조류의 종조성 비율은 홍조류(58.1%) > 대롱편모조류(갈조류, 25.8%) > 녹조류(14.5%) > 현화식물(1.6%) 순이었다. 시기별 해조류의 우점종은 3월과 6월에 나곡(F-1)과 후정(F-2) 해역에서 대롱편모조류(Ochrophyta, 갈조류)의 미역(Undaria pinnatifida)이 가장 우점하였다. 9월과 11월은 봉평(F-3) 해역과 공세항(F-C)에서 각각 홍조류(Rhodophyta)인 우뭇가사리(Gelidium elegans)와 혹돌잎류(Lithophyllum sp.)가 가장 우점하였다. 군집분석에서는 그룹이 계절에 따른 특정 해조류의 출현 유무에 따라 2개(A, B)로 나누어졌다. 우점종은 그룹 A에서 미역, 우뭇가사리, 미끈뼈대그물말, 그룹 B는 주로 혹돌잎류가 출현하였다. 따라서, 연구지역의 해조류 종조성과 군집구조는 전형적인 수온에 따른 계절변화와 함께 대조구와의 유의미한 차이도 보이지 않아서 산불에 의한 영향은 나타나지 않았다.

해조양식장 수질환경 모니터링을 통한 이산화탄소 단순 수지모델

심정희,강동진,한인성,권정노,이용화,Shim, Jeong Hee,Kang, Dong-Jin,Han, In Sung,Kwon, Jung No,Lee, Yong-Hwa 한국해양학회 2012 바다 Vol.17 No.4

부산시 기장군 일광 해조류양식장에서 2011년 7월 5일부터 6일까지 약 30시간동안 해양 표층수의 수온, 염분 등의 환경인자와 pH와 이산화탄소분압($fCO_2$)을 연속 관측하였다. 표층수의 수온과 염분은 $12.5{\sim}17.6^{\circ}C$, 33.7~34.0범위를 보였으며, 조석과 광주기, 해류 등의 영향으로 일변화 및 일간변화를 크게 나타내었다. 이산화탄소분압과 pH는 381~402 ${\mu}atm$, 8.03~8.15범위를 보였으며, 엽록소는 0.8~5.8 ${\mu}g\;L^{-1}$ 범위를 보였다. 이산화탄소분압, pH 그리고 엽록소는 최대 간조와 성층이 강했던 5일 오후 5시 전후에 최소 및 최고치를 보였으며, 이는 엽록소에 의한 생물생산 결과 이산화탄소는 낮고 pH는 높아진 것을 의미한다. 해조양식장 이산화탄소변화에 대한 단순 수지모델을 적용한 결과, 낮에는 생물생산에 의한 감소가 수온상승, 대기와의 교환에 의한 증가와 상쇄하는 것으로 나타났으며, 밤에는 대기와의 교환 물리적 혼합에 대한 과대 평가로 관측치보다 다소 높게 나타났다. 모델결과는 해조양식장 이산화탄소분압 총변화량의 14~40%는 해조류의 일차생산에 의한 것으로 나타났다. Real-time monitoring for environmental factors(temperature, salinity, chlorophyll, etc.) and carbonate components( pH and $fCO_2$) was conducted during 5-6th of July, 2012 at a seaweeds farm in Gijang, Busan. Surface temperature and salinity were ranged from $12.5{\sim}17.6^{\circ}C$ and 33.7~34.0, respectively, with highly daily and inter-daily variations due to tide, light frequency(day and night) and currents. Surface $fCO_2$ and pH showed a range of $381{\sim}402{\mu}atm$ and 8.03~8.15, and chlorophyll-a concentration in surface seawater ranged 0.8~5.8 ${\mu}g\;L^{-1}$. Environmental and carbonate factors showed the highest/lowest values around 5 pm of 5th July when the lowest tidal height and strongest thermocline in the water column, suggesting that biological production resulted in decrease of $CO_2$ and increase of pH in the seaweed farm. Processes affecting the surface $fCO_2$ distribution were evaluated using a simple budget model. In day time, biological productions by phytoplankton and macro algae are the main factors for $CO_2$ drawdown and counteracted the amount of $CO_2$ increase by temperature and air-sea exchange. The model values were a little higher than observed values in night time due to the over-estimation of physical mixing. The model suggested that algal production accounted about 14-40% of total $CO_2$ variation in seaweed farm.

남극 킹조지섬 마리안소만에서 침강 입자와 금속 플럭스의 계절 변화

심정희,한명우,김동선,강영철,정호성,이상훈 한국해양과학기술원 2002 Ocean and Polar Research Vol.24 No.2

Seasonal variations of settling particles and metal fluxes were monitored at a nearshore site of Marian Cove, King Geroge Island, Antarctica from 28th February 1998 to 22nd January 2000. Near-bottom sediment traps were deployed at 30 m water depth of the cove, and sampling bottles were recovered every month by SCUBA divers. Total particulate flux and metal concentrations were determined from the samples. Total particulate flux showed a distinct seasonality, high in austral summer and low in austral winter: the highest flux (21.97 g m2d1) was found in February of 1999, and the lowest (2.47 g m2d1) in September of 1998, when sea surface was frozen completely. Lithogenic particle flux accounted for 90% of the total flux, and showed a significantly negative correlation with the thickness of snow accumulation around the study site. It was suggested that the most of the lithogenic particles trapped in the bottles was transported by melt water stream from the surrounding land. Fluxes of Al, Fe, Ti, Mn, Zn, Cu, Co, Ni, Cr, Cd, and Pb showed similar seasonal variations with the total flux, and their averaged fluxes were 34000, 9000, 960, 180, 13.8, 17.6, 3.0, 2.1, 5.4, 0.02, and 1.5 nmol m2d1, respectively. Among the metals, Cu and Cd showed the most noticeable seasonal patterns. The Cd flux correlated positively with the fluxes of biogenic components while the Cu flux correlated with both the lithogenic and biogenic particle fluxes. The Cu flux peak in the late summer is likely related to a substantial amount of inflow of ice melt water laden with Cu-enriched lithogenic particles. On the other hands, the Cd flux peak in the early spring may be associated with the unusually early occurred phytoplankton bloom.

말하기·듣기 본질과 평가의 방향

심정희 서울교육대학 국어교육과 1994 초등 국어교육 Vol.- No.4

중년 후기 여성의 체형 유형화에 관한 연구

심정희 한국의류학회 2002 한국의류학회지 Vol.26 No.1

The purpose of this study was to classify the somatotype of late middle-aged women and to analyze the characteristics of each somatotype. The subjects were 337 late middle-aged women and their age range os from 45 to 59 years old. Data were collected through anthropometry and photometry and analyzed by factor analysis, cluster analysis and discriminant analysis. The results were as follows; 1. The result of factor analysis indicated that 9 factors were extracted through factor analysis and those factors comprised 83.56 percent of total variance. 2. Using factor scores, cluster analysis was carried out and the subject were classified into 4 cluster. Each cluster was classified as their body front and side view contour. Type 1 is tall, slim, and lower back is flat on the side. Type 2 is standard and lean-back type on the side. Type 3 is standard height and weight, H type in front, and belly-protruded on the side. Type 4 is short, fat, and the side is hip-protruded. 3. According to the stepwise discriminant analysis, the 9 important items in classifying the somatotype of the late middle-aged women are as follows ; lower back tilt angle, hip depth(back) - back waist depth(back), bust depth(fore) - anterior waist depth(fore), jugular fossa point(fore), upper back tilt angle, bust breadth - waist breadth, right shoulder tilt, height of shoulder - height of anterior waist, abdomen breath. The correct classification rate for these items is as exact as 84.62%.

중년 전기 여성의 체형 유형화에 관한 연구

심정희 한국의류학회 2001 한국의류학회지 Vol.25 No.8

The purpose of this study was to classify and analyze the somatotype of early middle-aged women and to provide it's total data for clothing construction, and to improve clothing culture. The subjects were 277 early middle-aged women between 35 and 44 years old. Data were collected through anthropometry and photometry and analyzed by factor analysis, cluster analysis and discriminant analysis. The results were as follows; 1. The result of factor analysis indicated that 10 factors were extracted through factor analysis and those factors comprised 86.13 percent of total variance. 2. Using factor scores, cluster analysis was carried out and the subject were classified into 4 cluster. Type 1 is tall, slim, and X type in front. Type 2 is standard height and weight, short upper body, and hip-protruded on the side. Type 3 is standard height, thin, H type in front, back and hip are clearly protruded, and lean-back type on the side. Type 4 is standard height, fat, and long upper body. 3. According to the stepwise discriminant analysis, the 8 important items in classifying the somatotype of early middle-aged women are as follows ; bust girth, back length, hip breadth-waist breadth, back protruded point depth(back)-back waist depth(back), hip tangent tilt, hip depth(back)-back waist depth(back), bust depth-waist depth, and cervical height. The correct classification rate for these items is as exact as 83.20%.