우질병에 있어서 경사시험관법에 의한 적혈구침항율에 관한 연구

신종욱,Shin Jong-uk 한국임상수의학회 1994 한국임상수의학회지 Vol.11 No.2

In the present studies, samples of blood were taken from 24 Holstein cattle over 2 years old. By using the angled tube method the normal ESR values were determined in cattle under various diseases. The results obtained were summarized as follows ; The standardized ESR/hr using 45$^{\circ}$ angled capillary tubes were 19.0% in case of necrotic mastitis, 23.0% in nephritis, 12.2% in anemia and 4.2% in diarrhea. And the ESR was increased from some necrotic disease, decreased from diarrhea conditions and general the normal ESRs were shown in the sub-clinical or recovery stage of infections or disorders.

유우의 유방염 치료에 대한 약제내성 검사

신종욱,Shin Jong.Uk 대한수의사회 1984 대한수의사회지 Vol.20 No.1

This study was undertaken to investigate drug susceptibility against the major antibiotics used in this country by the use of the disc diffusion susceptibility test. The results are summarized as follows: 1. Comparatively highly sensitive to antibiotics e

경사관법(傾斜管法)에 의한 우혈액(牛血液)의 적혈구침강율(赤血球沈降率) 측정(測定)

이방환,신종욱,Lee, Bang-whan,Shin, Jong-uk 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.1

The measurement of angled erythrocyte sedimentation rate (ESR), as a replacement for perpendicular ESR, for cattle blood was scrutinized since it has been well known that perpendicular ESR in cattle is too slow to be adopted as an effective clinical test. Samples of blood were taken from 186 Korean native cattle over 2 years old. The results obtained in the experiment were summarized as follows. 1. Average values of perpendicular ESR/24hrs in 15 apparently healthy cattle, as measured by Wintrobe, Westergren and capillary tubes, were $5.8{\pm}2.2$, $11.1{\pm}3.7$ and $10.4{\pm}4.5%$ respectively, which were found to be similar to the values of perpendicular ESR/hr of normal blood of human. 2. The ESR was determined in the tubes held at 90, 75, 60, 45, 30 and 15-degree angles, using 3 types of tubes. For the diagnostic purposes, the best results were obtained from the tubes held at 45-degree angle. 3. The angled ESR values increased as the diameters of the tube-bores decreased. 4. The tube length did not affect the angled ESR(%). 5. The angled ESR values increased with the increased environmental temperature during the ESR measurement. 6. The storage temperature at $5^{\circ}C$, $20^{\circ}C$ and $35^{\circ}C$, of the blood for 24 hours did not affect the angled ESR. 7. Samples of blood were treated with 4 kinds of anticoagulants (heparin, $K_2$-EDTA, double oxalate and sodium citrate) and the ESR was determined at 45-degree angle, using capillary hematocrit tubes. The ESR values were higher in the blood samples treated with sodium citrate than in those treated with other anticoagulants. 8. By using the autologous plasma, the PCV was adjusted to be 5, 10, 20, 30, 40 and 50ml/100ml and the ESR was determined in the capillary hematocrit and Wintrobe tubes held at 45 degrees. In both of the methods the ESRs increased as the values of PCV decreased. The regressions of ESR to PCV in both 45-degree-angled capillary and Wintrobe tubes were curvilinear. For the capillary hematocrit tubes the second degree polynomial $Y=61.9779-2.3533x+0.0228x^2$ (r=0.9999) fits the data. And in the case of Wintrobe tubes the second degree polynomial $Y=27.9767-1.1314x-0.0117x^2$ (r=0.9998) fits the data. 9. The 45-degree angled ESR was determined in the blood of 71 healthy Korean native cows using capillary hematocrit tubes. The average PCV was $35.4{\pm}3.6ml/100ml$. The observed ESR/hr averaged $7.2{\pm}2.7%$, while the corrected ESR/hr to a PCV of 36ml/100ml averaged $6.6{\pm}1.3%$. From these results it was concluded that to obtain the best results the ESR/hr of Korean native cattle should be determined at 45-degree angle at room temperature($20^{\circ}C$) using capillary hematocrit tubes.

육성우의 혈액학치 및 혈액화학치에 관한 연구

강정부,신종욱,Kang Chung-Boo,Shin Jong-Uk 대한수의사회 1985 대한수의사회지 Vol.21 No.6

This experiment was performed to investigate the levels of hematological and blood chemical parameters in korean native growing cattle from 7 month to 11 month after birth. Animals used were 15 healthy korean native growing cattle with about 190kg body we

신생자돈의 선천성 전지과골증의 발생예

곽수동,신종욱,최상룡,Kwak Soo-Dong,Shin Jong-Uk,Choe Sang-Yong 한국임상수의학회 1987 한국임상수의학회지 Vol.4 No.2

Two cases of congenital hyperostosis at a pig farm were observed and examined pathologically in Landrace piglets. Twenty two piglets from 2 litters: 10 alive piglets in a litter and 2 stillborn and 10 alive piglets in another, were born with thickened forelimbs and some of them with thickened hindlimbs too. These piglets stowed symptoms of staggering and incoordination. Only one of these piglets could be reared normally and all the others died within 3 days of age. The thickened parts of their limbs were felt firm and not well pitted by finger pressing. An apparent ossification was identified on the central regions of the cross sections of the thickened limbs, and some pale, gray and ossiferous signs were found in the peripheral soft areas. On histopathological examination of the thickened limbs, it was found that the bone trabeculae were newly proliferated in a concentric form in central regions and in a radiated form in peripheral regions. The peripheral regions of the periosteum and perichondrium of new bone were proliferated with connective tissue and the edematous muscle bundles were atrophic and degenerative. Both the sows which produced piglets of congenital hyperostosis were mated with a different boar at next breeding and farrowed normal piglets. It was concluded that the swine congenital hyperostosis in the present cases was expressed by autosomal recessive gene of first mating boar.

Enzyme immunoassay(EIA)에 의한 소의 progesterone 측정과 이의 응용에 관한 연구 II. Progesterone 측정에 대한 효소면역측정방법(酵素免疫測定方法)의 확립

강정부,신종욱,최상용,Kang, Chung-boo,Shin, Jong-uk,Choe, Sang-yong 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.1

This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, enzyme conjugate and gelatin were invested. The sensitivity, recovery rate and reproducibility by this assay were also analyzed. The results obtained were as follows: 1. The suitable supplementation level of gelatin was 0.2%. As the gelatin level increased to 1%, coefficient variation of interassay was shown to be irregular. 2. The optimum dilution rate of enzyme conjugate was 30 times. Enzyme activity was greatly fluctuated depending on the minor condition of enzyme conjugate technique. Therefore, it was considered to be checked when determined. 3. The sensitivity of the assay was 12 pg/tube. 4. Recovery rate was decreased when the amount of sample was too little or too much, but the recovery rate was high as 97.8% when the amount of sample between 50 and $200{\mu}l$. 5. Mean intra-assay and inter-assay coefficient of variation was 4.5% and 5.9%, respectively. By using liquid phase double antibody enzyme immunoassay, progesterone in plasma can be detected, and also this assay system is applicable to study on physiological function of progesterone and to diagnosis of reproductive disorders.

Enzyme immunoassay(EIA)에 의한 소의 혈중 progesterone 측정과 이의 응용에 관한 연구 I. 이항체(二項體)의 최적조건에 관한 연구

강정부,신종욱,최상용,Kang, Chung-boo,Shin, Jong-uk,Choe, Sang-yong 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2

This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated. The optimum dilution rate of first antibody, second antibody and normal rabbit serum was $10{\times}10^3$ to $15{\times}10^3$, 20 and $1{\times}10^3$ times, respectively.

경사모세관법(傾斜毛細管法)에 의한 산양혈액(山羊血液)의 적혈구침강율(赤血球沈降率) 측정(測定)

신성식,이방환,신종욱,Shin, Sung-shik,Lee, Bang-whan,Shin, Jong-uk 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.1

The purpose of this study is to develop a new method which enables the goat ESR to be used as an effective clinical test. Blood samples were taken from 61 Korean native goats aged above one year old and the effect of tube inclinations, tube bores, tube lengths, environmental temperatures during tests and packed erythrocyte volumes (PCV) on the ESR were observed. The results were summarized as follows. 1. The ESR/hr using capillary hematocrit tube (Micro-Ht-tube) was gradually increased as the tube angle inclined from 90 (vertical) to 15 degrees and the best angle in view of both security and fast sedimentation rate was found to be an angle of 45 degrees. 2. The 45-degree angled ESR ($45^{\circ}$-ESR) increased as the diameter of tube bore decreased. 3. The tube length did not affect the $45^{\circ}$-ESR in percent. 4. The $45^{\circ}$-ESR increased with the increased environmental temperature during the ESR test. 5. The heparinized Micro-Ht tubes did not affect the $45^{\circ}$-ESR of EDTA-blood in healthy group but in anemic group. In the anemic group, the ESRs by the heparinized Micro-Ht-tubes were slightly higher than those by non-heparinized Micro-Ht-tubes. 6. By using the autologous plasma, PCV of the blood was adjusted to be 10, 20, 30, 40 and 50ml/100ml and $45^{\circ}$-ESRs were determined in the Micro-Ht-tubes. The $45^{\circ}$-ESRs increased as the values of PCV decreased. The regression of the $45^{\circ}ESR$ to PCV was curvilinear with the second degree polynomial, $Y=42.1838-1.7355X+0.0180X^2$(r=0.9997). 7. The $45^{\circ}$-ESR/hr, using non-heparinized Micro-Ht-tubes at $20^{\circ}C$, was determined in 35 healthy Korean native goats. The average PCV was $30.6{\pm}1.4ml/100ml$. The observed ESR values averaged $6.8{\pm}1.7%$ and the corrected ESR values to the standard PCV of 31ml/100ml averaged $6.5{\pm}1.2%$. From these results, the angled capillary tube method was found to be desirable ESR test of goat blood in which EDTA-blood is filled in nonheparinized Micro-Ht-tubes held at an angle of 45 degrees for an hour at $20^{\circ}C$.

소 혈액의 45도-경사-Wintrobe관에 의한 적혈구침강율 측정에 있어서 환경온도 및 적혈구침층용적치에 상관하는 관계적 예기 적혈구침강율

이방환,박영우,신종욱,Lee, Bang-whan,Park, Young-woo,Shin, Jong-uk 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1

Each of twenty blood samples taken from apparently healthy Korean cows was used to produce five different mixtures of autologous plasma and blood corpuscles such that their values of packed cell volume(PCV) lay between 10 to 50ml/100ml. The measurement of erythrocyte sedimentation rate (ESR) using 45 degree-angled Wintrobe hematocrit tube, 3mm bore, ($45^{\circ}$-Wintrobe-ESR) was practiced for the blood of various levels of PCV under the ambient temperature of $10^{\circ}C$, $20^{\circ}C$ and $30^{\circ}C$. Correlation of the ESR to the ambient temperature showed linear regression each in five levels of PCV. The ESR increased with ascending ambient temperature, and magnitude of the increase of the ESR became greater as the level of PCV lowered. Correlation of the ESR to PCV showed curvilinear regression each in three levels of the ambient temperature, and the ESR was increased with decreasing PCV. The data were statistically analysed and a list of relative anticipated $45^{\circ}$-Wintrobe-ESR values for PCV and ambient temperature was presented.

건유기 유방염 감염우의 유방내 면역저하요인 규명에 관한 연구 II. 호중구에 의한 말초혈액 및 유즙 림프구의 mitogen 유도성 증식반응 억제작용

신동백,박용호,남향미,문진산,주이석,신종욱,Shin, Dong-baek,Park, Yong-ho,Nam, Hyang-mi,Moon, Jin-san,Joo, Yi-seok,Shin, Jong-uk 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3

To establish the effective ways to prevent bovine mastitis, the study has been performed to investigate the attributable factors causing down-regulation of immune responses in mammary gland of non-lactating cows. Lymphocytes from peripheral blood and mammary gland secretions(MGS) were obtained from normal healthy cows and mastitic cows, respectively. Lymphoblastogenesis were investigated carefully by adding different concentrations of supernatants collected from pure-cultures of neutrophils seperated from peripheral blood and MGS, respectively. The results obtained are as follows ; 1. Lymphoblastogenesis activity stimulated by Con A, PWM and PHA were significantly reduced in MGS from mastitic cows. 2. Supernatants collected from pure-culture of neutrophils separated both from peripheral blood and MGS showed inhibitory effect on mitogenic lymphoblastogenesis. 3. Supernatants from mammary gland neutrophils have shown 7 times more inhibitory activity than those from peripheral blood and this inhibitory effect was increased in proportion to increasing concentrations of supernatants when those were added to lymphoblast cells in culture.