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강수경,신미경,어규식,전양현,홍정표,Kang, Soo-Kyung,Shin, Mi-Kyoung,Auh, Q-Schick,Chun, Yang-Hyun,Hong, Jung-Pyo 대한안면통증구강내과학회 2007 Journal of Oral Medicine and Pain Vol.32 No.1
Plant extract has attracted considerable interest in oral disease therapy. The present study was performed to observe the antibacterial effect on cariogenic Streptococcus mutans GS5 and Streptococcus sobrinus 6715, and periodontopathic Actinobacillus actinomycetemcomitans Y4 of phytoncide from Chamaecyparis obtusa Sieb. et Zucc employing the measurement of optical density, viable cell counts, and antibiotic sensitivity. The results were as follows: 1. Minimum inhibitory concentration of the phytoncide for S. mutans, S. sobrinus, and A. actinomycetemcomitans was observed to be 0.5%, 1%, and 0.2%, respectively. 2. Minimum bactericidal concentration of the phytoncide for S. mutans, S. sobrinus, and A. actinomycetemcomitans was determined to be 0.5%, 2%, and 0.2%, respectively. 3. The bacteria exposed to the phytoncide become more sensitive to antibiotics. The phytoncide enhanced significantly antibacterial activity of ampicillin against S. mutans and S. sobrinus. It also increased significantly the activity of penicillin and amoxicillin against S. sobrinus. In contrast, the phytoncide augmented the activity of amoxicillin and cefotaxime against A. actinomycetemcomitans but the increase was not statistically significant. The overall results indicate that phytoncide from Chamaecyparis obtusa Sieb. et Zucc used for this study has a strong antibacterial activity against cariogenic and periodontopathic bacteria and that it also has permeabilizing effect on certain antibiotics against these bacteria. Therefore, the phytoncide may be used as a candidate for prevention and therapeutic agent against oral infectious disease including dental caries and periodontal disease.
김선규,신미경,어규식,이진용,홍정표,전양현,Kim, Sun-Q,Shin, Mi-Kyoung,Auh, Q-Schick,Lee, Jin-Yong,Hong, Jung-Pyo,Chun, Yang-Hyun 대한안면통증구강내과학회 2007 Journal of Oral Medicine and Pain Vol.32 No.2
Trees emit phytoncide into atmosphere to protect them from predation. Phytoncide from different trees has its own unique fragrance that is referred to as forest bath. Phytoncide, which is essential oil of trees, has microbicidal, insecticidal, acaricidal, and deodorizing effect. The present study was performed to examine the effect of phytoncide on Porphyromonas gingivalis, which is one of the most important causative agents of periodontitis and halitosis. P. gingivalis 2561 was incubated with or without phytoncide extracted from Hinoki (Chamaecyparis obtusa Sieb. et Zucc.; Japanese cypress) and then changes were observed in its cell viability, antibiotic sensitivity, morphology, and biochemical/molecular biological pattern. The results were as follows: 1. The phytoncide appeared to have a strong antibacterial effect on P. gingivalis. MIC of phytoncide for the bacterium was determined to be 0.008%. The antibacterial effect was attributed to bactericidal activity against P. gingivalis. It almost completely suppressed the bacterial cell viability (>99.9%) at the concentration of 0.01%, which is the MBC for the bacterium. 2. The phytoncide failed to enhance the bacterial susceptibility to ampicillin, cefotaxime, penicillin, and tetracycline but did increase the susceptibility to amoxicillin. 3. Numbers of electron dense granules, ghost cell, and vesicles increased with increasing concentration of the phytoncide, 4. RT-PCR analysis revealed that expression of superoxide dismutase was increased in the bacterium incubated with the phytoncide. 5. No distinct difference in protein profile between the bacterium incubated with or without the phytoncide was observed as determined by SDS-PAGE and immunoblot. Overall results suggest that the phytoncide is a strong antibacterial agent that has a bactericidal action against P. gingivalis. The phytoncide does not seem to affect much the profile of the major outer membrane proteins but interferes with antioxidant activity of the bacterium. Along with this, yet unknown mechanism may cause changes in cell morphology and eventually cell death.
최유경 ( Yu Kyoung Choi ),김도훈 ( Do Hoon Kim ),신미경 ( Mi Kyong Shin ),김영생 ( Young Saeng Kim ),김종훈 ( Jong Hun Kim ),구본호 ( Bon Ho Ku ),최정희 ( Jeong Hee Choi ) 대한천식알레르기학회 2007 천식 및 알레르기 Vol.27 No.4
Occult foreign body aspiration is uncommon in adults because there is usually a high index of suspicion. Occult foreign bodies can remain undetected for months to years and are often misdiagnosed as asthma, pneumonia, or even malignancy. We here in report a rare case of occult bronchial foreign body aspiration in an 81-year-old woman presenting with asthma-like symptom for 8 months. (Korean J Asthma Allergy Clin Immunol 2007;27:273-276)
Bacillus sp. SP-KSW3을 이용하여 제조한 간장의 발효 기간에 따른 효소 활성 및 기능성의 변화
김병수 ( Byoung Soo Kim ),이창호 ( Chang Ho Rhee ),홍영아 ( Young Ah Hong ),권태형 ( Tae Hyung Kwon ),신미경 ( Mi Kyoung Shin ),김진희 ( Jin Hui Kim ),우철주 ( Cheol Joo Woo ),김영배 ( Young Bae Kim ),박희동 ( Heui Dong Park ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2008 한국식품저장유통학회지 Vol.15 No.2
金南姬,申美敬 성신여자대학교 기초과학연구소 1989 基礎科學硏究誌 Vol.6 No.-
Most component of honey is saccharides, and honey has a small amount of protein, amino acids, vitamines, minerals, etc. There are many studies of hydroxymethylfurfural in honey. In these experiments, the ratio of fructose/glucose and the content of saccharides of Korean acacia and chestnut honey were measured by HPLC, and the content of proline, a kind of amino acids in the honey, was measured by UV/VIS. Spectrophotometer. Also, the change of diastase activity on the temperature and storage time were experimented. In order to study of some rheological properties, the steady state viscosity was observed on the shear rate, the dynamic viscosity and shear modulus were surveyed on the frequency by Rheolography of cone-and-plate type. Also, the dynamic viscosity to the change of the temperature and storage time on the constant frequency was observed. As a result, honey contained a large amount of fructose, glucose and sucrose. The ratio of fructose/glucose effected on the crystallization of honey was measured, and each values were 1.17 and 1.15. At 4℃, room temperature (20℃-25℃) and 37℃, diastase activity showed no decrease with the temperature and storage time. But at 50℃, 70℃ and 80℃, it showed an appreciable decrease of diastase activity. From these experiments, two kinds of honey represented the rule of Newtonian liquid. The change of viscosity values of two kinds of honey on the temperature and storage time decreased in a higher temperature than the lower. This result agreed with the equation of Andrade.
초인지 수업모형이 초등학생들의 분자개념 변화에 미치는 효과
최영재,고영신,신미경 한국초등과학교육학회 1999 초등과학교육 Vol.18 No.2
The purpose of this study was to find out the effectiveness of metacognitive instruction model on the changes of science concepts, when it was applied to 6th grade students. To do this, students were tested with the achievement of molecules and molecular motion concepts and metacognitive self-regulation test as a pretest. Based upon metacognitive instruction model and student's conception, instruction program were developed. This metacognitive strategy Program was applied to the experimental group and expository teaching was applied to the comparison group (followed the order and method in authorized science textbook and teacher's handbook). When planned lessons were finished, students were given a post-test to find conceptual change. After six months students were given a test again to find retention effect. There was a significant difference in conceptual change and retention between comparison group and experimental group by treatment at p$lt;.05 level. The difference between comparison group and experimental group was especially significant, when the situation of test item wasn't similar to that of the textbook. Metacognitive instruction model was more effective to high group than low group in metacognitive self-regulation level on conceptual chance and retention. So the metacognitive strategy Played an important role in conceptual change and retention. And we can recognize that the students who take part in the metacognitive lessen can apply the corrected concept to the other concrete situation because they can understand new concept accurately by metacognitive strategies. And we can guess that high group in metacognitive self-regulation level can team metacognitive strategy easily but relatively low 7:roue students have some trouble in learning new strategy.