다채 (Brassica campestris var. narinosa) 유묘의 형질전환 및 일시발현의 정량적 분석

신동일,박희성,Shin Dong-Il,Park Hee-Sung 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.4

Brassica campestris var. narinosa는 새싹채소로 애용되는 식물로서 간단한 용기에서 손쉽게 재배할 수 있다. 본 연구에서는 vacuum-infiltration을 통한 Agrobacterium-mediated transformation에 의해서 B. campestris var. narinosa 싹에서의 GUS일시발현을 성공적으로 확인하였다. 발아전의 종자가 발아를 이미 시작한 종자보다 그 형질전환 효율이 높게 나타났다. 한편, hydrogen peroxide를 2일 생장시킨 싹에 처리한 후 형질전환 하였을 때 GUS발현이 증가되는 것을 관찰하였다. 간염항원유전자의 형질전환과 ELISA에 의한 항원단백질의 발현량 분석 시 hydrogen peroxide 처리 싹이 비처리 형질전환 싹에서 보다 2배 이상의 발현량이 측정되었다. Tah tasai chinese cabbage (Brassica campestris var. narinosa), a vegetable plant popularly consumed as several-days-old seedlings in oriental countries, can be easily cultivated using a simple appliance. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration (agroinfiltration) resulted in a successful transient GUS gene expression in tah tasai chinese cabbage seedlings. Pre-germinated seeds were found to be more susceptible to Agrobacterium infection than one-day-old or two-days-old seedlings. We also demonstrated that hydrogen peroxide (HPO) treatment increased GUS expression especially for two-days-old seedlings. In ELISA using seedlings transformed with hepatitis B surface antigen (HBsAg) DNA by agroinfiltration, HBsAg protein synthesis increased more than two folds by HPO treatment to two-days-old seedlings in comparison to the mock-treated pre-germinated seeds.

Sodium Hypochlorite Solution As a Chemical Wounding Agent for Improving Agrobacterium-mediated Chinese Cabbage Seed Transformation

신동일,박희성,Shin Dong-Il,Park Hee-Sung Korean Society of Life Science 2005 생명과학회지 Vol.15 No.6

배추 종자 및 유묘에 대하여 GUS발현 또는 hepatitis B surface antigen (HBsAg)발현 벡터를 지니는 Agrobacterium tumefaciens LBA4404 세포를 이용하여 진공침윤(agroinfiltration)에 의한 형질전환을 시도하였다. 특히 ELISA를 이용한 HBsAg발현의 정량적 분석에서 agroinfiltration방법은 형질전환효율이 매우 저조하게 나타났다. 그러나 차아염소산나트륨 용액을 발아 전 또는 발아 중인 배추종자에 처리한 후 agroinfiltration을 실시한 경우 형질전환 효율이 $2\~5$배 증가하였다. 따라서 차아염소산나트륨 등의 화학연마제에 의한 종자의 상처발생이 Agrobacterium의 감염을 용이하게 함으로써 배추유묘에서의 일시유전자발현을 증대시키는 것으로 제안되고있다. Chinese cabbage (Brassica campestris ssp. napus var. pekinensis Makino) seeds/seedlings were transformed via vacuum-infiltration with recombinant Agrobacterium tumefaciens LBA4404 cells. The agroinfiltration method was determined to be unsuccessful for Chinese cabbage transformation during the analysis of hepatitis B surface antigen expression by ELISA. However, treatment of sodium hypochlorite solution, prior to agroinfiltration, to pregerminated or germinating 1 day- or 2 days-old seeds was proven effectively to enhance transformation efficiency, suggesting that chemical wounding caused by sodium hypochlorite reaction might facilitate Agrobacterium infection and, therefore, transient gene expression in Chinese cabbage sprouts.

고구마 뿌리절편에서의 ${\beta}-glucuronidase$ 일시발현

신동일,박희성,Shin, Dong-Il,Park, Hee-Sung 한국응용생명화학회 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.4

문항개발 과정에서 시험작성 세부계획서의 역할 연구

신동일(Shin, Dong- Il),김나희(Kim, Na-Hee) 한국외국어교육학회 2010 Foreign languages education Vol.17 No.1

The purpose of this paper is to investigate the features of test development process from different components of test specifications. A data-driven study was planned to value the positive roles of test specifications in item development and review process. The source of data was collected from test writers' and reviewers' meetings in the development of a writing English test. All the key words or phrases were categorized and also counted by frequency. It was then found that there was mismatch between what was equally valued in the test specification and what was frequently discussed in testers' meetings. It is suggested that test specifications need to be discussed and implemented in the earlier stages of test development. Also, it should be noted that participation in test specifications is by those most closely connected with and affected by the test use.

워크플로우 관리 시스템의 설계 및 구현

신동일(Dong Il Shin),신동규(Dong Kyoo Shin) 한국정보처리학회 2000 정보처리학회논문지 Vol.7 No.5

Workflow means the automation of a business process, in which documents, information or tasks are transferred among participants for business action according to a set of procedural rules, and workflow management system is a system which defines, creates and manages the execution of workflow running one or more workflow engines. In this research, necessity of standardizing current workflow systems is recognized, and problems such as interoperability of current systems, dynamic adaptation to changing business environment and lacking of assessment, management and auditing of business processes are analyzed so that the design and implementation of a workflow system is focused on to offer a solution to the problems. The system is designed and implemented to change dynamically on Run-Time the processes definition defined on Build-Time, and interoperability is enabled by developing workflow engine and related modules based on the WfMC specifications and APIs.

유채유묘의 형질전환을 통한 일시발현시스템의 개발

신동일(Dong-Il Shin),박희성(Hee-Sung Park) 한국생물공학회 2006 KSBB Journal Vol.21 No.6

언어학적 전환, 비판적 언어학 전통, 그리고 비판적 담론연구의 출현

신동일(Dong il Shin) 한국질적탐구학회 2018 질적탐구 Vol.4 No.3

본 연구의 목적은 비판의식이 보태진 담론연구방법의 출현 배경과 지적 토대를 탐색하고 질적연구 분야에 어떻게 기여하고 어떤 한계점을 갖고 있는지 살펴보는 것이다. 비판적 담론연구를 위해 실증적 자료를 기획하고 수집하고 분석하는 연구방법 역시 학자군마다 다소 차이가 크기 때문에 본 연구는 알튀세(Althusser)의 이데올로기, 그람시(Gramsci)의 헤게모니. 푸코(Foucault)의 권력/담론/지식, 홀(Hall)의 문화연구로부터 구축된 구조주의와 언어학적 전환(linguistic turn)의 지식전통과 바흐친(Bakhtin), 폐슈(Pêcheux), 할러데이(Halliday)를 포함한 비판적 언어학(critical linguistics) 연구전통으로부터 페어클러프(Fairclough)의 담론연구 모형이 어떻게 출현할 수 있었는지 우선적으로 탐색하기로 한다. 페어클러프의 모형은 할러데이의 체계기능문법(systemic functional grammar)으로부터 차용된 텍스트 분석방법, 바흐친의 얽힌 텍스트의 해석, 사회구조 분석에 용이한 푸코의 고고학/계보학 이론, 그람시의 헤게모니적 투쟁논점을 모두 결합시키면서 미시적 분석과 거시적 분석의 상호 간 단점을 보완했다. 본 연구는 비판적 담론분석의 연구방법적 의미와 함께 한계점 역시 부연하고 추후 연구자들이 숙지해야 할 점을 정리했다. The purpose of this study is to provide theoretical and methodological resources to understand how CDS (Critical Discourse Studies) has been emerged as a research methodology. In this review of literature, the theoretical foundations will first be discussed on the tradition of linguistic turn and structuralistic movement, including Althusser’s ideology, Foucault’s power and discourse, Gramsci’s hegemony, Hall’s cultural study, as well as the tradition of critical linguistics including Halliday’s systematic functional grammar, and Bakhtin’s intertextuality. CDS clearly intends to incorporate social theories into discourse analysis and advocates social commitment and interventionism in researches. The main features and domains of CDS are discussed in this paper, with emphasis on the theory formation (how texts and discourses work in ideological interests with powerful political consequences) by one of CDS’s most prominent scholars, Norman Fairclough. Th e strengths as well as the limitations of the Fairclough’s model-based research are also discussed. In the conclusion, the article makes some suggestions for future directions of CDS methodological development.

영문학서사 기반의 스토리 창작 지원도구의 모형개발 연구

신동일(Shin, Dong-il),김은정(Kim, Eun-Jung),한만수(Hahn, Man-Soo),황수경(Hwang, Su-Kyung) 인문콘텐츠학회 2015 인문콘텐츠 Vol.0 No.36

본 연구의 목적은 영문학 작품의 서사에서 발견되는 모티프를 기반으로 데이터베이스를 구축하고 이를 바탕으로 스토리를 창작할 수 있는 지원도구의 개발모형을 제안하는 것이다. ‘STORY PLAYER’라고 이름을 붙인 영문학 서사 기반의 스토리 창작 지원도구는 온·오프라인 연동이 가능한 3가지 형태, 즉, 보드게임, 모바일 앱, 웹 플랫폼 환경에서 게임처럼 스토리를 만들 수 있도록 하며 웹 공간에서 자신이 만들고 있는 스토리를 타인과 공유하거나 편집하도록 돕는다. 자아형성기의 중요한 단계에 진입한 아동 혹은 청소년들이 게임의 환경에서 자기주도적인 서사를 창작하도록 유도하고 디지털 매체에 익숙한 사용자에게 마치 서사 창작을 놀이처럼, 또한 놀이를 서사를 통해 접근할 수 있는 창작의 환경을 제공한다. 본 연구는 영문학 작품의 서사가 연구자들에 의해 비평과 분석의 대상으로 주로 다뤄지면서 인문콘텐츠로서의 가치가 저평가된 점을 주목했다. 본 연구의 개발모형은 서사양식의 확장과 공유를 가능하게 하는 디지털 미디어 환경 위에서 제안된 것이기 때문에 관련 연구자들이 이야기 산업경제의 활성화에 참여할 여지를 찾게 할 것이다. 또한 완성도 높은 양질의 스토리텔링 콘텐츠를 실질적으로 개발해보고자 하는 동기를 학계에 부여하고 디지털 매체의 적극적인 도입을 통해 탈고전적 (생성)서사 담론을 실험적으로 적용해 볼 수 있는 탈학제적 융합 연구의 저변도 확대시킬 것으로 기대된다. The purpose of this research is to suggest a development model of story-creating tool, in which a motif-based database is constructed on the works of English literature. The ‘STORY PLAYER,’ a tool assisting story creation serves as three interworking forms of board game, mobile application, and web platform environment and encourages users to edit their own stories and share them on and off-line with the other storytellers. It is expected that STORY PLAYER helps children or adolescents who enter the crucial stage of forming their identities practice their self-directed narratives in the game-pursuing environment. The generation familiar with digital media can access the process of story creation as a game entertainment. This research attempts to make an interdisciplinary approach to narrative studies of English literature. The works of English literature have been researched often for literary criticism and analysis, and its potential as digitalized contents has been underestimated. The development model suggested in this paper will facilitate the expansion and sharing of storytelling practice in the digital environment and will hopefully contribute to vitalize storytelling-related industry. The academia are then invited to participate in the development of storytelling contents and also to experiment post-classical narratology/creation discourses through digital media.

국내 외국인 거주자의 언어권리의 침해에 관한 연구

신동일 ( Dong Il Shin ),심우진 ( Woo Jin Shim ) 이중언어학회 2013 이중언어학 Vol.51 No.-

Investigating the cases of violating linguistic human rights in Korea. Bilingual Research 51, 151-180. The purpose of this study is to examine the case of linguistic human rights violations to minority groups in Korea. First of all, this research defines the concept of ``linguistic human rights`` and explores its background. Then, three types of minority (foreign students, foreign workers and immigrant wives) were grouped. All the related newspaper articles, which appeared between 2003 and 2012, were collected over the Chosun Ilbo and Donga Ilbo Internet archives. Although this research cannot detect detailed cases against linguistic human rights, but affords an opportunity to consider the meaningfulness of linguistics human rights against the three minority groups. This article is concluded by proposing further research topics on linguistic diversity, ecological perspectives of language development. (Chung-Ang University)

언어교육의 후기-현대성에 관한 인문학적 고찰 -맥커뮤니케이션, 자아정체성, 그리고 생태주의 관점에서부터

신동일 ( Dong Il Shin ) 한국영어영문학회 2013 영어 영문학 Vol.59 No.1

In recent decades there has been a tendency to frame communicative language abilities as a set of technical skills that can be defined, quantified, measured, predicted and ultimately controlled. This process is often called ``McCommunication`` (Ritzer 2003). The over-rationalized, standardized, and commodified education of language have dominated the current conventions of developing curriculum, materials, and tests of language learning. Authentic language and its educational activities have often conformed to narrow standards of correctness, clarity, efficiency, or ``verbal hygiene`` (Cameron 1995). Korean researchers in the field of second language education have not taken the issue of McCommunication culture seriously. The purpose of this paper is to re-visit the modernization issues of language education industry by referencing late-modernity (Beck 1997; Giddens 1997) and ecological views (Kramsch 2002; van Lier 2004) across the other academic disciplines. It was argued that the McDonaldizatin of language learning has become a common phenomenon, and that the meaningfulness has been seriously questioned. Then Giddens` (1991) concepts of reflexivity and self-identity were suggested as a meaningful ends to language learning. Ecology of language learning were also elaborated as a transdisciplinary endeavor, to encourage reflection over the McDonaldized culture of (English) language learning in Korea. Further research questions, such as appropriate pedagogy (Kramsch & Sullivan 1996), expanded modeling of communicative language abilities (Norton 2000), are also discussed.