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Ganoderma lucidum WK-003 균사체 액체배양으로부터 균체외 고분자물질의 생산조건과 간 보호 효과
송치현,양병근,전용재,나경수,손동환,김혁일,김영환 한국생물공학회 1998 KSBB Journal Vol.13 No.4
The optimum conditions of the production of exo-polymer by Ganoderma lucidum WK-003 and it's hepatoprotective effect was studied. Optimum conditions for the production of exo-polymer (3.18 g/$\ell$) by using shaken flask culture of G. lucidum WK-003 were pH 4.5, 30$^{\circ}C$, 120 rpm for 18 days cultivation. Also exo-polymer productio (7.15 g/$\ell$) was optimized by 5$\ell$ jar fermenter cultivation with condition of pH 4.5, 30$^{\circ}C$, 200 rpm, 1.0 vvm for 6 days cultivation. Glutamic pyruvic transaminase(GPT) activities in serum of intoxicated Sprague-Dawley rat were decreased from 704 IU/L to 330 IU/L by oral administration of the exo-polymer (20mg/kg$.$day)f or 4 consecutive days.
송치현,김준영,박경덕,장용현,이석종,방용준,전재복,이원주 대한의진균학회 2019 대한의진균학회지 Vol.24 No.3
Background: Tinea capitis is a contagious fungal infection that occurs predominantly in childhood. Although its incidence has declined, its epidemiological and mycological characteristics are continue to evolve. Objective: To assess changes in the epidemiological and mycological characteristics of tinea capitis in teenage patients in Southeastern Korea. Methods: We retrospectively investigated epidemiological and mycological characteristics of 202 teenage patients with tinea capitis who visited Kyungpook National University Hospital and the Catholic Skin Disease Clinic from 1989-2018. Results: Of 202 patients, 177 patients showed KOH-positivity. Dermatophytes were cultured from 157 patients. The annual incidence of tinea capitis ranged from 0 to 30 between 1989 and 2018. Of 202 patients, 153 patients (75.74%) were male and 49 patients (24.26%) were female. The ratio of male to female was 1:0.32. For seasonal distribution, 78 patients (38.61%) visited our hospital in winter, 59 patients (29.21%) visited in spring, 35 patients (17.33%) visited in fall, and 30 patients (14.85%) visited in summer. Microsporum canis was the most common dermatophyte (44.06%) isolated from tinea capitis. Trichophyton tonsurans was the second most common dermatophyte (26.73%). For inhabitancy distribution, 151 patients (74.75%) lived in urban areas and 51 (25.25%) lived in rural areas. Conclusion: The epidemiological characteristics of teenage patients with tinea capitis were distinct from those of adults in annual incidence, sexual distribution and isolated dermatophytes. Trichophyton tonsurans was the main causative organism of tinea capitis in recent 10 years. These results are useful for the targeted treatment and prevention of tinea capitis.
담배 이분(泥粉)으로부터 향을 생성하는 효모의 분리, 동정 및 그 향의 관능적 특성에 관한 연구
송치현,강은희,박은수,Song, Chi-Hyeun,Kang, Eun-Heuy,Park, Eun-Su 한국연초학회 1981 한국연초학회지 Vol.3 No.2
From various sources of natural habitat yeasts, which coo 14 transform the constituents of tobacco Powder into flavors, were isolated and three isolates. YOII, M4-1, and M 19-1, were selected These were identified, by their biological characteristics, as Hansenuia ciferri, Pichia acaciae and Candida tropicalis, respectively. Their ferments were extracted with petroleum ether and fractionated into basic, neutral and acidic fractions. The major organoleptic properties of the fermented flavors were detected in the neutral fractions by the sensory test and the yields and threshold values of those fractions were determined and the organoleptic characteristics were described as well.
송치현,이용세,장태현,Md. Rezuanul Islam 한국잔디학회 2012 Weed & Turfgrass Science Vol.26 No.1
The objective of this study was to identify bacterial antagonists of R. solani AG2-2 (IV) on zoysiagrass and to evaluate their antifungal activity in vitro and in vivo to select an antagonistic isolate. Antagonistic isolates that inhibit large patch disease caused by R. solani AG2-2 (IV) in zoysiagrass were selected from several soils,and their antagonistic activities were investigated in vitro and in vivo. Of 216 bacterial isolates, 67 inhibited several plant pathogenic fungi. The isolates that inhibited stem-segment colonization by R. solani AG2-2 (IV) in zoysiagrass were tested in a growth chamber. Eleven isolates were active as plant growth promoting isolates. Among them, five plant growth promoting isolates and their concentration dependent efficiency on zoysiagrass following inoculation with R. solani AG2-2 (IV) was evaluated. Isolate H33 was one of the potential antagonistic isolates, and it was further tested against various plant pathogens. H33 not only suppressed the disease caused by R. solani AG2-2 (IV) on zoysiagrass but also promoted leaf weight and leaf height of zoysiagrass under growth chamber and greenhouse conditions. The H33 isolate, which belongs to Streptomyces arenae, was identified through physiological, biochemical, and 16S rDNA studies. Further studies will investigate the cultural characterization of S. arenae H33 and isolation and identification of antifungal substance produced by S. arenae H33.