Effect of cold-acclimation on freezing tolerance in canola (Brassica napus L.)

Moon Tae Song(宋文台),로렌스코플랜드(Lawrence O. Copeland) 한국육종학회 1995 한국육종학회지 Vol.27 No.2

Of the many factors influencing winter survival of plants, freezing tolerance is one of the most important. This study was conducted to determine the relationship between in vitro freezing tolerance and winter survival of canola (Brassica napusL.). Six winter cultivars and one spring cultivar that had previously exhibited a broad range of winter survival were examined both in a controlled environment chamber and under field conditions. Freezing tests showed that cold acclimation increased the freezing tolerance of both winter and spring cultivars, and that the differences in freezing tolerance among cultivars were due to their ability to acclimate. Freezing tolerance measured by electroleakage tests was highly correlated with winter survival. This suggests that the electroleakage test can be used to predict the comparative winter survival of canola cultivars and tools for the selection of winter hardy cultivars in plant breeding.

Expression of two-cold regulated genes of canola responding to cold acclimation

Moon Tae Song(宋文台),로렌스코플랜드(Lawrence O. Copeland) 한국육종학회 1995 한국육종학회지 Vol.27 No.1

Cold acclimation increases freezing tolerance and the changes of gene expression may involve in the enhancement of freezing tolerance during cold acclimation. This study was conducted to determine the responses of two cold-regulated genes in canola (Brassica napus L.), BN28 and BN115, to cold acclimation and find out correlation between increasing of freezing tolerance and the expression of the genes. Six winter cultivars and one spring cultivar that had previously exhibited a broad range of winter survival were examined in a controlled environment chamber. Southern blot analysis revealed that the seven cultivars examined had the same copy number of BN28 and BN115. Northern blot analysis indicated that these genes were induced within one day by cold temperature and turned off immediately by deacclimation. Though time-course increase of transcript in one of the genes was highly correlated with the time-course increase of freezing tolerance, no correlation was found at the cultivar lever at specific time of measurement. However, this does not rule out the possible role of cold-regulated genes in freezing tolerance of canola.

벼의 자포니카형간 교배에서 유래된 F₂ 집단을 이용한 연관지도 작성 및 출수 관련 유전자의 QTL 분석

송문태(Moon Tae Song),이점호(Jeom Ho Lee),조윤상(Youn Sang Cho),정오영(O . Young Jeong),황흥구(Hung Goo Hwang) 한국유전학회 2001 Genes & Genomics Vol.23 No.4

Most of the molecular linkage maps in rice were constructed using segregating populations derived from crosses between parents with a wide genetic distance, such as japonica and indica rices, because of the high polymorphic profiles in them. However, the molecular markers in genetic maps in the populations of japonica/japonica or indica/indica are more useful in practice since these markers can be utilized directly in the concurrent breeding programs. This study aims to develop a genetic map using a population derived from early heading japonica cultivar Olbyeo1 and late heading cultivar Ilpumbyeo. Plant height and heading dates were checked in 300 F2 plants, from which randomly selected sixty plants were analyzed with DNA markers. One hundred and twenty nine polymorphic markers were detected in the segregating population and fifty markers were located in seven linkage groups. Total genetic distance of the seven linkage group was 795.5 cM with an average 17 cM distance between markers. Two QTL for heading days were detected in the linkage group 4 whose individual LOD values were 4.06 and 6.69, respectively. Genetic synteny of the seven linkage groups in the present study is being surveyed with the established rice linkage maps with more molecular markers.

느타리버섯과 잔나비걸상버섯과의 이목간 원형질체 융합

유영복,송문태,고승주,유창현,차동열,박용환,장권열 ( Young Bok Yoo,Moon Tae Song,Seung Joo Go,Chang Hyun You,Dong Yeul Cha,Yong Hwan Park,Kwon Yawl Chang ) 한국균학회 1989 韓國菌學會誌 Vol.17 No.3

통일형 X 인디카 벼 품종간 RIL 집단을 이용한 몇가지 양적형질의 유전자좌 분석

조윤상(Youn Sang Cho),이점호(Jeom Ho Lee),송문태(Moon Tae Song),김남수(Nam Soo Kim) 한국육종학회 2000 한국육종학회지 Vol.32 No.2

방목과 비료시용(肥料施用)이 산지초지개량에 미치는 효과

최진룡,송문태,김원영 경상대학교 축산진흥연구소 1986 畜産振興硏究所報 Vol.13 No.1

In order to investigate the effects of grazing and fertilization on the improvement of oversown pasture on the hill country, an experimental site was selected from the northern aspect of Namwon Branch of the National Animal Breeding Institute. Each plot, sized 15㎡, was fenced and native vegetations were grazed by nine sheep for three days to reduce the density of native vegetation and to open space for introduced pasture. Before sowing, lime(1.5t per ha) and phosphorus(690g per plot as super phosphate) were fertilized. Selected cultivars of Dactylis glomerata, Festuca arundinacea, Trifolium repens and Trifolium pratense were sown at the rates of 3.4kg seeds for grasses and 0.6kg for legumes per 180㎡. Legume seeds were inoculated before sowing. After germination, the grazing plot were grazed by sheep with 20 to 30 day-intervals. Herbage yield and botanical composition were measured with one-month interval after wintering. Herbage yield increased significantly in the grazed plots and the yield was doubled by fertilization. The yield of fertilized plots without grazing was comparative to that of grazed plots without fertilization. There was a significant improvement in the botanical composition, i. e. , 20 per cent grasses before improvement turned out 86 per cent introduced grasses at the end of three years from sowing on both grazed and fertilized plots. However, the improvement was retarded up to 35 per cent in the stated plots without fertilization. The initial 7 per cent of legumes increased up to 50 per cent in the grazed and fertilized plots, which is not comparable to 15μ per cent for grazed without fertilization. High proportion of native shrubs(73 per cent) rapidly decreased up to 2.3 per cent after three years from sowing. Conclusively, it was envisaged that the introduced pasture plants showed better response to grazing and fertilization, and that the hill land can be .improved effectively by fencing, fertilization and regular grazing immediately after seed germination.

한국산 벼 품종 판별에 있어서 세가지 PCR 방법들간의 효용성 비교

이강수,송문태,정오영,홍지현 한국유전학회 1999 Genes & Genomics Vol.21 No.3

The effectiveness of three kinds of PCR primers, random, microsatellite, and sequence tagged site (STS) for the fingerprinting of genetically similar Korean rice cultivars, was estimated by comparing the resolution powers of these primers to classify a set of 40 Korean rice cultivars. Among the three kinds of primers examined, the random primer showed higher resolution than the other two primer kinds to make the classification of Korean rice cultivars in condition that the agarose gels were used for separation of the bands. The dendrogram constructed from pooled data from three different PCR-methods gave a clear distinctions not only between the two cultivar groups, japonica rice and indica/japonica hybrid rice groups but also between individual cultivars within a cultivar group. The near grouping of genetically similar rice groups having similar breeding backgrounds suggests that PCR techniques can offer a reliable and effective means of assessing not only genetic variation in diverse rice germplasm but also cultivar identification between very close cultivars.

일본 벼 품종의 Allelopathy 탐색과 전망

최진룡,송문태,조영손,송미광홍 한국국제농업개발학회 2000 韓國國際農業開發學會誌 Vol.12 No.1

Allelopathy has a potential in integrated weed management as a weed control strategy. Crop plants generally have the capability to exude allelochemicals into their surroundings, which can suppress the growth of weeds in their vicinity. In this paper extentive discussions were made on the agents of allelopathic activity and major research activities of allelopathic effects of rice in Japan, A series of experiments on selection for superior genotypes with allelopathic potential have been carried out with several rice cultivars or germplasms. Approaches to crop production that successfully reduce weed seed production can make farmers benefit by reducing management inputs and costs. Japanese allelopathy research team established a new testing method for the allelopathy potential in rice with the combination of biological test and field test. Among them, monochoria test was found to be most valuable for rice allelopathy test. Some old native rice cultivars of Pakistan, Philippines, and India were found to show high allelopathic potential than new cultivars of America and Japan. Upto date, although progress has been made in understanding the genetics of allelopathic activity of crop and successful genetic manipulation, further researches are required for a full degree of understanding of the genetic control of allelopathic activity in rice. Once the allelopathic genes are identified, a breeding program could be initiated to transfer the genes into modern cultivars and enhance their allelopathic activity in order to maximize weed suppression effects. Allelopathic research in rice should be cooperated with the research team of crop ecologists, soil scientists, agronomists, biochemists. plant physiologist, and farmers. This interest can be extended to effectiveness of cover crops, which is able to evaluate the allelopathy potential in the paddy field.

일본에서의 농산물과 다이옥신 현황

최진룡,최재원,송문태,조영손 한국국제농업개발학회 2000 韓國國際農業開發學會誌 Vol.12 No.1

Considerable interest has been focused on dioxins recently. In Germany since 1994, interdisciplinary and consummer-related investigations under the guidance of the Federal Ministry for Food. Agricultural and Forestry has reported the occurrence of polychlorinated dibenzoioxines and dibenzoflurances (called dioxins hereinafter) in foodstuffs and the contamination of foods by chemicals. In Japan, the government have given attention to the chemicals by enforcing the emergency laws for solving of dioxins from 1997. These case indicated the serious situation of the pollution from the dioxins. In this report, the chemical properties of dioxins, dioxins contents in several food stuff, the contamination pathway of dioxins to the food stuff and the nature of the degradation of the chemicals were broadly discussed. Special attention was also given to the problems by the chemicals and the solving methods of the agricultural practices in Japan. A particular interest is given to dioxins research team in Ehime University involved in investigating the content of the dioxins in the paddy soil and pathway of the chemicals. Their research effort have attributed to the finding of the contamination of Japanese paddy soil with the chemicals as well as the contents of the chemicals is considerably higher than safe standards. The major concern in present is that there will be no agricultural field with dioxins free and consequent exposure of men to the those hazardous chemicals through food-web system. The origins of the dioxins in the farm soil and agricultural product may be mostly from the herbicides and other agricultural chemicals. The chemicals may be accumulated in the plant and animal from the those contaminated soil and water. Half-time periods of dioxins reduction is longer than controllable and the cost of purification of the chemical from soil is considerably high. In conclusion, an imminent development and application of farming practice reducing dioxins in our agricultural environment is badly needed in order to conserve the earth and humankind in future.

RAPD , AFLP , SSRP 를 이용한 벼 품종간 변이 분석 및 이들 마커기법들간의 효율성 비교

홍순관,조윤상,김남수,송문태,이점호,문헌팔 한국유전학회 1998 Genes & Genomics Vol.20 No.2

Studies on the genetic differentiation of two different ecotypes, Oryza sativa japonica and indica, are very important for many aspects including the overcoming sterility barriers in hybrids between two types in cultivared rice. This study was carried out to 1) investigate the genetic variation and differentiation among 34 rice cultivars in two ecotypes using PCR-based markers, RAPD, AFLP and microsatellite-SSR and 2) compare the results of genetic variations derived from each PCR-marker technique. The indica type rices were clustered separately from japonica type rices at the genetic similarity level of 0.75. The genetic similarity among the indica rices was lower than that of japonica type rices. Three cultivars, Fuji269, Sobaegbyeo. Keumobyeo showed very close genetic relatedness since they were indistinguishable in our experiment. Since the utilized PCR-based marker techniques are the most frequently used ones in many areas, the variations detected by each marker were compared with each other. Although differences were not significant in genetic relatedness derived from RAPD, AFLP and microsatellite-SSR separately, AFLP technique was proved to be very effective in detecting genetic variation since it can generate many marker bands in short time.