http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Induction of thermogenic adipocytes: molecular targets and thermogenic small molecules
송노준,장서혁,Dean Y Li,Claudio J Villanueva,박계원 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Adipose tissue is a central metabolic organ that controls energy homeostasis of the whole body. White adipose tissue (WAT) stores excess energy in the form of triglycerides, whereas brown adipose tissue (BAT) dissipates energy in the form of heat through mitochondrial uncoupling protein 1 (Ucp1). A newly identified adipose tissue called ‘beige fat’ (BAT-like) is produced through a process called WAT browning. This tissue mainly resides in WAT depots and displays intermediate characteristics of both WAT and BAT. Since the recent discovery of BAT in the human body, along with the identification of molecular targets for BAT activation, stimulating energy expenditure has been considered as a great strategy to treat human obesity and metabolic diseases. Here we summarize recent findings regarding molecular targets and thermogenic small molecules that can stimulate BAT and increase energy expenditure, with an emphasis on possible therapeutic applications in humans.
Sulfuretin Prevents Obesity and Metabolic Diseases in Diet Induced Obese Mice
김수지,송노준,장서혁,반가희,최유리,이동권,윤의정,최진희,이전,유재혁,신동한,박기문,강희,이석찬,구진모,조윤신,박계원 한국응용약물학회 2019 Biomolecules & Therapeutics(구 응용약물학회지) Vol.27 No.1
The global obesity epidemic and associated metabolic diseases require alternative biological targets for new therapeutic strategies. In this study, we show that a phytochemical sulfuretin suppressed adipocyte differentiation of preadipocytes and administration of sulfuretin to high fat diet-fed obese mice prevented obesity and increased insulin sensitivity. These effects were associated with a suppressed expression of inflammatory markers, induced expression of adiponectin, and increased levels of phosphorylated ERK and AKT. To elucidate the molecular mechanism of sulfuretin in adipocytes, we performed microarray analysis and identified activating transcription factor 3 (Atf3) as a sulfuretin-responsive gene. Sulfuretin elevated Atf3 mRNA and protein levels in white adipose tissue and adipocytes. Consistently, deficiency of Atf3 promoted lipid accumulation and the expression of adipocyte markers. Sulfuretin’s but not resveratrol’s anti-adipogenic effects were diminished in Atf3 deficient cells, indicating that Atf3 is an essential factor in the effects of sulfuretin. These results highlight the usefulness of sulfuretin as a new anti-obesity intervention for the prevention of obesity and its associated metabolic diseases.
장재율,장서혁,송다운,송노준,한새로아름,오승준,윤의정,안지인,이석찬,구진모,박계원 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.12
Butea monosperma (Lam.) Taub. has been applied to treat inflammatory, metabolic, and infectious diseases. However, the antiobesity effects of B. monosperma (Lam.) Taub. flower (BMF) and the underlying mechanisms have not been determined. In this study, we analyzed the various extraction procedures, investigated the antiobesity effects, and identified the main chemical constituents of BMF. The BMF was subjected to acid hydrolysis in 5% H2SO4 in methanol at 50°C for 48 h and partitioned with ethyl acetate. The acid-hydrolyzed BMF ethyl acetate extracts (BMFE) strongly induced the expression of uncoupling protein 1 (Ucp1) and other thermogenic genes in C3H10T1/2 adipocytes. Daily oral administration of 70 mg/kg BMFE (BMFE70) to mice with diet-induced obesity resulted in less body weight gain, increased glucose tolerance, higher rectal temperature, and increased oxygen consumption. Qualitative and quantitative analyses along with treatments in Akt1 knockout mouse embryonic fibroblasts indicate that butein is a major active ingredient of BMFE, which stimulates Ucp1 gene expression. These data show the effects of butein-containing B. monosperma flower extract on thermogenesis and energy expenditure, further suggesting the potential role of BMFE as a functional ingredient in obesity and related metabolic diseases.
Woo-Seok Jang,송노준,윤향진,Min-Ji Kim,구진모,홍정우,이영민,박기문,박계원 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.5
Screening of wild vegetable extracts led to isolation of Sedum kamtschaticum, Lythrum anceps, and Astilbe chinensis var. davidii as putative anti-adipogenic herbal extracts. Cell viability,lipid accumulation, and adipocyte marker expression were determined to validate the effects of the selected extracts on adipogenesis. All extracts suppressed lipid accumulation in C3H10T1/2 cells, primary mouse embryonic fibroblasts, and 3T3-L1 cells in a dose-dependent manner. These extracts inhibited mRNA expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its downstream target genes such as adipocyte protein 2 (aP2), lipoprotein lipase, adiponectin, and CD36. Treatment with these extracts also consistently suppressed PPARγ and aP2 protein expressions. Additionally, these extracts generated from different parts and/or various harvest periods similarly inhibited adipocyte differentiation. Results showed the extracts have potential beneficial roles in obesity and related metabolic syndromes.
김민지,장우석,이인경,김종근,성기승,서초롱,송노준,방민혁,이영민,김행란,박기문,박계원 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.7
Pathological increases in adipogenic potential with decreases in osteogenic differentiation occur in osteoporotic bone marrow cells. Previous studies have shown that bioactive materials isolated from natural products can reciprocally regulate adipogenic and osteogenic fates of bone marrow cells. In this study, we showed that Eupatorium japonicum stem extracts (EJE) suppressed lipid accumulation and inhibited the expression of adipocyte markers in multipotent C3H10T1/2 and primary bone marrow cells. Conversely, EJE stimulated alkaline phosphatase activity and induced the expression of osteoblast markers in C3H10T1/2 and primary bone marrow cells. Daily oral administration of 50 mg/kg of EJE for 6 weeks to ovariectomized rats prevented body weight increase and bone mineral density decrease. Finally, activity-guided fractionation led to the identification of coumaric acid and coumaric acid methyl ester as bioactive anti-adipogenic and pro-osteogenic components in EJE. Taken together, our data indicate a promising possibility of E. japonicum as a functional food and as a therapeutic intervention for preventing osteoporosis and bone fractures.