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송근상 ( Gun Sang Song ),노경환 ( Kyung Hwan Noh ),문영래 ( Young Lae Moon ),유재원 ( Jae Won You ),송창훈 ( Chang Hoon Song ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
Cytokines involve in both inflammatory and healing mechanism. The aim of this study to evaluate the changing nature of cytokines and thereby appropriate time for using bone marrow derived monuclear cells(BMMNC) to acquire best effect. We chose 8 patients whose age is ranged from 38 to 51(mean=49). Bone marrow aspirate was obtained from iliac bone of patients with consented to the procedure. Cytokines from plasma layer acquired after 30 minutes of centrifugation at 1,820rpm were analyzed. Cytokines were evaluated after abstraction of mononuclear cells using ficoll method. The cytokines evaluated were Eotaxin, GM-CSF, IFN r, IL-1 a, IL-1 bIL-10, IL- 12(p40), IL-12(p70),IL-13, IL-15, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, MCP-1, MIP-1a, RANTES and TNF-a. High level of IP-10, IL-12 p40, RANTES were detected after centrifugation. Additionally, high level of IL- 6, IL-7, IL-8, MCP-1, MIP-1a were noticed in 1 and 7 days following culture. Mononuclear cells derivedfrom bone marrow have high activity character and the change of cytokine was observed after simple culture. Our results show that bone marrow derived mononuclear cells immediately after centrifugation of bone marrow is most effective.
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