경북 동해안 환경에서 분리된 V. parahaemolyticus 및 V. vulnificus의 생태학적 및 항생제 감수성 특성

손진창,박승우,민경진 한국환경보건학회 2003 한국환경보건학회지 Vol.29 No.2

This study was carried out to investigated the distribution and characteristics of Vibrio spp. isolated from fish and shellfish, seawater and brackish water samples collected from Pohang, Uljin, Yeongduk and Gyeongju in Gyeongbuk Province from April 2000 to October 2000. Total 155 strains of genus Vibrio were isolated from 439 collected samples, and numbers of isolated strains of V. parahaemolyticus and V. vulnificus were 140 and 15, respectively. The isolation rate from the samples collected in Pohang was the highest as 41.5% (76 strains), and wat the highest as 71.4% (30 strains) in brackish water, and was the highest as 55.7% (34 strains) in August. And the optimal pH, temperature, and NaCl concentration for growth of V. parahaemolyticus and V. cholerae were 8.0, 3$0^{\circ}C$ and 2.0%, respectively. In a resistance test for environmental factors, heat and cold resistants of V. parahaemolyticus were higher than those of V. vulnificus, withstanding for 15 minutes at 6$0^{\circ}C$ and 6 days at -18$^{\circ}C$. The pH range for existence of V. parahaemolyticus and V. vulnificus were 4.5~l1.0 and 4.5~10.0, showing the similar resistance to pH. V. parahaemolyticus and V. vulnificus could grow in media containing up to 10.0% and 7.0% NaCl, respectively, Salt-tolerance of V. parahaemolyticus was higher than that of V. vulnificus. In an antibiotics sensitivity test against 16 strains of V. parahaemolyticus, twelve strains were resistant to ampicillin, eight strains were resistant to cephalothin. one strain was resistant to streptomycin, and one strain was resistant to ticarcillin.

이고들빼기 및 고들빼기 에탄올 추출물 첨가식이가 급성 에탄올 투여 흰쥐의 혈청과 간지질 및 알코올 대사 효소활성 변동에 미치는 영향

손진창(Jin-Chang Son),김성환(Sung-Hwan Kim),이상일(Sang-Il Lee),이예경(Ye-Kyung Lee),김순동(Soon-Dong Kim) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.2

민간에서 널리 이용되고 있는 이고들빼기 및 고들빼기 성분이 에탄올의 급성독성에 어떠한 영향을 미치는지 검토하고자 실험동물을 정상대조군(NC), 알코올 대조군(EC), 이고들빼기 잎 에탄올추출물 0.5% 첨가식이군(YDL), 이고들빼기 뿌리 에탄올추출물 0.5% 첨가식이군(YDR), 고들빼기 잎 에탄올추출물 0.5% 첨가식이군(YSL), 고들빼기 뿌리 에탄올추출물 0.5% 첨가식이군(YSR) 등 6군(7마리/군)으로 나누어 4주간 사육한 다음, 급성적으로 에탄올을 경구투여 하여 간 조직 ADH 및 ALDH의 활성 변동과 혈청 및 간조직 지질함량 변동 그리고 혈청 ALT의 활성 변동을 관찰하여 다음과 같은 결과를 확인하였다. 급성에탄올 경구투여에 의해서도 간조직 ADH의 활성은 모든 실험군에서 유의한 변동은 없었으나, ALDH의 활성은 에탄올에 의해 유의하게 감소하였고, 잎 추출물 첨가식이군인 YDL 및 YSL군에서는 EC군에 비해 유의하게 증가하였다. 그리고 YDL 및 YSL군에서는 에탄올의 혈중 농도가 EC군에 비해 유의하게 감소하였다. 또한 급성에탄올 투여로 증가하였던 혈청 TG와 total cholesterol 함량은 모든 실험식이군에서 유의하게 감소하였으며, 증가되었던 LDL-cholesterol의 함량은 YSR군을 제외한 모든 실험식이군에서 유의하게 감소하였다. 그러나 감소되었던 HDL-cholesterol의 함량은 YSR군에서만 EC군에 비해 유의하게 증가하였다. 한편, 간조직 TG 및 total lipid 함량은 모든 실험군에서 EC군에 비해 감소하였으나, 간조직 cholesterol의 함량은 YDL군에서만 EC군에 비해 유의하게 감소하였다. 그리고 간조직 손상의 지표인 혈청 ALT의 활성은 급성에탄올 처치에 의해 현저하게 증가하였으나, 모든 실험식이군에서 대조군 수준으로 회복되었다. 이상의 실험결과로 보아 이고들빼기 및 고들빼기 성분이 급성에탄올 독성을 완화시켜줄 수 있을 것으로 생각되며, 이들 성분들을 이용한 기능성식품의 개발에 대한 기초자료로 활용할 수 있을 것이다. This study examined the protective effects of an ethanol extract of Youngia denticulata leaf (YDL) and Youngia denticulata root (YDR), and Youngia sonchifolia leaf (YSL) and Youngia sonchifolia root (YSR) on acute ethanol- intoxicated rat. The rats were pretreated with an ethanol extract of YDL, YDR, YSL and YSR for 4 weeks before being exposed to ethanol (5 g ethanol, po/kg BW). The biochemical indices (hepatic alcohol metabolic enzymes and serum ALT activities, and hepatic and serum lipid profiles) were examined to evaluate the protective effects. The hepatic ADH activities in all experimental groups were not changed significantly by acute ethanol after a pretreatment with the YS and YD ethanol extracts. In contrast, the ALDH activity in EC (ethanol control) was higher than that of NC (normal control); these activities in the YDL and YSL groups were significantly higher than that of the EC group. On the other hand, acute ethanol exposure resulted in a significant increase in the serum TG, total cholesterol, LDL-cholesterol, hepatic TG, total lipid and cholesterol levels, and serum ALT activity, and a decrease in the serum HDL-cholesterol. A pretreatment with the YS and YD ethanol extracts dramatically attenuated these adverse effects. In particular, the YDL pretreatment markedly suppressed the ethanol- induced increase in the serum and hepatic TG and total cholesterol levels. Furthermore, serum ethanol was decreased by a pretreatment with YSL, YSR, YDL, or YDR. Overall, YD and YS ethanol extracts attenuate acute ethanol-induced hyperlipidemia and fatty liver significantly. Nevertheless, further study will be needed.

Kudoa ogawai (Myxosporea: Kudoidae) Infection in Cultured Olive Flounder Paralichthys olivaceus

신상필,진창남,손한창,이제희 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.4

Since Kudoa septempuntata was identified as a causative agent of food poisoning associated with raw olive flounder Paralichthys olivaceus, interest and concern regarding the parasite have increased. However, there have been no investigations or reports of other Kudoa species infecting the fish (except for K. paralichthys, which infects the brain) in Korea. We found cysts filled with myxospores of Kudoa species in muscles of cultured olive flounder specimens and identified these to the species level. Mature spores were quadrate, measuring 8.7±0.5 μm in length, 9.2±0.4 μm in thick- ness, and 12.9±0.6 μm in width. The spores containing 4 polar capsules had a length of 2.1±0.2 μm and a width of 1.8 ± 0.3 μm. The partial 18S and 28S rDNA of isolates showed 99-100% similarities with K. ogawai. Using these morpho- logical and molecular analyses, the species was identified as K. ogawai. This study is the first report of K. ogawai infection in cultured olive flounder in Korea.

경상북도 내 유통 농산물 중의 잔류농약 동향 (2004~2008년)

양승태,손진창,정광현,이창일,김미정,박희숙,차춘근 한국식품위생안전성학회 2009 한국식품위생안전성학회지 Vol.24 No.4

The content of residual pesticides in commercial agricultural products in Gyeongsangbuk-Do area was investigated for 5 years extending the year 2004 through 2008. The detection rates of residual pesticides in agricultural products by year were similar in range of 11.6~16.4%. But the violation rates showed lower values in the last years from 4.5% of the year 2004 to 0% of the year 2007. The highest residual concentration of each pesticide detected in commercial agricultural products was investigated by year. That is, in the year 2004 and 2005, chlorpyrifos, chlorothalonil, diazinon, endosulfan, ethoprophos, fenarimol and procymidone were detected over the tolerance in kale, parsley, celery, chard and lettuce, and in the year 2006, permethrin in the soybean and peanut. The detection rate and violation rate of pesticides were highly increased in the order of the endosulfan, chlorpyrifos, procymidone, chlorfenapyr, fenitrothion, imazalil, isoprothiolane, methidathion and permethrin. The detection rate and violation rate of pesticides were increased after August every year.

Sinuolinea capsularis (Myxosporea: Sinuolineidae) Isolated from Urinary Bladder of Cultured Olive Flounder Paralichthys olivaceus

신상필,진창남,손한창,이제희 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.2

Sinuolinea capsularis Davis, 1917 is myxosporean that infect the urinary system of the host fish. Insufficient mor- phological and molecular data of S. capsularis exits, and it is therefore difficult to make an accurate identification of the parasite. We tried a series of morphological and molecular analysis to identify an myxosporean isolated from urinary blad- der of cultured olive flounder, Paralichthys olivaceus, from Jeju island in the Republic of Korea. Some of them were ob- served under a light microscope and SEM, and remain samples were used molecular and phylogenetic analysis. Mature spores were subspherical, measuring 13.9±0.6 μm in length and 13.8±0.8 μm in width. Two spherical polar capsules on opposite sides in the middle of the spore had a diameter range of 4.3±0.4 μm. Scanning electron microscopy revealed that spores a severely twisted the suture line. By the morphological comparison and analysis, it was identified as S. capsu- laris. In addition, we obtained the partial 18S rDNA of S. capsularis and first registered it in NCBI. Phylogenetic analysis showed that S. capsularis clustered with Zschokkella subclade infecting the urinary system of marine fish, and it supported the infection site tropism effect on phylogeny of marine myxosporeans as well as the origin of Sinuolinea is not monophyly.

구강병인균에 대한 마와 꿀풀추출물의 항균?항우식효과

정기옥,민경진,손진창 한국환경보건학회 2007 한국환경보건학회지 Vol.33 No.2

Yam, Prunella was stepwise extracted with hexane, chloroform, ethyl acetate, butanol, and water. Anti-microbial activity of each extract was investigated. Hexane extract was tested for anti-microbial effect on Streptococcus mutans, one of causative factor of dental caries. Methanol extracts of 7 plants were investigated to anti-microbial effects on S. mutans KCTC 5316, P. gingivalis KCTC 5352, S. aureus KCTC 1927 by means of agar diffusion method. Methanol extract of Yam and Prunella revealed anti-microbial activity against S. mutans, P. gingivalis, and S. aureus. Also, hexane fraction of Yam revealed anti-microbial activity against S. mutans. In sequence of hexane, chloroform, ethylacetate, butanol fraction by Prunella acted as potent anti-microbial agent on P. gingivalis The measured MIC of hexane fraction of Yam and Prunella on S. mutans KCTC 5316 strain was 0.25mg/ml and 0.5mg/ml and the MIC of hexane fraction of Prunella on S. aureus was 0.5mg/ml. The hexane fraction of Yam and Prunella suppressed viable cell counts(VCC) of S. mutans, especially after 24 hrs. The Prunella hexane fraction suppressed VCC of S. aureus, after 12 and 24 hrs. Tested concentrations were 0.1, 0.25 and 0.5mg/ml. the results were compared with control (0mg/ml). The pH of S. mutans media and GTase activity were determined to evaluate the anticariogenic activity of Yam, Prunella hexane fraction. The pH were increased from 5.6 to 7.0-7.2 in concentration of 2.0mg/ml. Yam hexane extraction revealed 35% inhibition to GTase activity and Prunella inhibited 25% of GTase. These results suggest that the hexane extracts of Yam and prunella have Antibacterial activities against S. mutans, P. gingivalis, S. aureus and have preventive effect on dental caries.

우리나라 부동산산업의 발전과정과 과제

이상영 ( Sang Young Lee ),손진수 ( Jin Soo Son ),진창하 ( Chang Ha Jin ),임재만 ( Jae Man Lim ) 한국부동산분석학회 2015 不動産學硏究 Vol.21 No.3

연구논문 / 목단피 추출물의 항균 및 항산화 작용

권오근(O Guen Kweon),손진창(Jin Chang Son),김상철(Sang Chul Kim),정신교(Shin Kyo Chung),박승우(Seung Woo Park) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 1998 한국식품저장유통학회지 Vol.5 No.3

작약 추출물의 구강병원균에 대한 항균성 및 구강암 세포 증식 억제효과

박현숙,민경진,차춘근,송진욱,손진창,Park, Hyun-Suk,Min, Kyung-Jin,Cha, Chun-Geun,Song, Jin-Wook,Son, Jin-Chang 한국환경보건학회 2007 한국환경보건학회지 Vol.33 No.1

Paeonia lactiflora was stepwise extracted with hexane, chloroform, ethyl acetate, butanol and water. Anti-microbial activity of each extract was investigated. Methanol extract of P. lactiflora revealed anti-microbial activity against S. mutans, C. albicans, and S. aureus. Also, hexane fraction revealed anti-bacterial activity against S. mutans and ethyl acetate fraction acted as potent anti-microbial agent on C. albicans and S. aureus. The relative growth ratio(RGR) of hexane fraction of P. lactiflora against S. mutans were determined as 77.8% in concentration of 0.125 mg/ml, 98.46% in 0.25 mg/ml and 100% in 0.5 mg/ml. The ethyl acetate fraction of P. lactiflora revealed RGR against C. albicans as 52.5% in concentration of 0.125 mg/ml, 60.83% in 0.25 mg/ml and 78.33% in 0.5 mg/ml. It indicate that increasing concentration increase RGR. The measured minimal inhibitory concentration(MIC) of hexane fraction on S. mutans KCTC 5316 strain was 0.5 mg/ml and MIC of ethyl acetate fraction on C. albicans KCTC 7270 was 2.0 mg/ml. The experiment of inhibition to growth of KB roll(oral squamous cell carcinoma) result 61.9% in butanol, 76.7% in hexane extract of P. lactiflora. The hexane extract exhibit potent inhibition effect to the growth of KB cell. These results suggest that the hexane extract of Paeonia lactiflora has antimicrobial activity against S. mutans and has preventive effect to dental caries in addition to potent inhibition to KB cell growth.

합성곱 신경망(CNN)을 이용한 U-Net 기반의 인공지능 안면 정면화 모델

이상민 ( Sangmin Lee ),손원호 ( Wonho Son ),진창균 ( Changgyun Jin ),김지현 ( Ji-hyun Kim ),김지윤 ( Jiyun Kim ),박나은 ( Naeun Park ),김가은 ( Gaeun Kim ),권진영 ( Jin Young Kwon ),이혜리 ( Hye Yi Lee ),김종완 ( Jongwan Kim ),오덕신 한국정보처리학회 2020 한국정보처리학회 학술대회논문집 Vol.27 No.2

안면 인식은 Face ID를 비롯하여 미아 찾기, 범죄자 추적 등의 분야에 도입되고 있다. 안면 인식은 최근 딥러닝을 통해 인식률이 향상되었으나, 측면에서의 인식률은 정면에 비해 특징 추출이 어려우므로 비교적 낮다. 이런 문제는 해당 인물의 정면이 없고 측면만 존재할 경우 안면 인식을 통한 신원확인이 어려워 단점으로 작용될 수 있다. 본 논문에서는 측면 이미지를 바탕으로 정면을 생성함으로써 안면 인식을 적용할 수 있는 상황을 확장하는 인공지능 기반의 안면 정면화 모델을 구현한다. 모델의 안면 특징 추출을 위해 VGG-Face를 사용하며 특징 추출에서 생길 수 있는 정보 손실을 막기 위해 U-Net 구조를 사용한다.