Establishment of optimal decellularization conditions using porcine placenta

손지형,김대중,이동목,서병부 사단법인 한국동물생명공학회 2021 한국동물생명공학회지 Vol.36 No.4

Organ transplantation is currently the most fundamental treatment for organ failure, but there is a shortage of organ supply compared to those in need. Regenerative medicine has recently developed a decellularization technique that overcomes the limitations of conventional organ transplantation and attempts to reconstruct damaged tissues or organs to their normal state. Several decellularization methods have been suggested. In this experiment, the decellularization methods were used to find effective decellularization methods for humanlike porcine placenta. The optimal conditions for decellular support are low DNA content and high glycos amino glycans (GAGs) and collagen content. In order to satisfy this condition, SDS and Triton X-100 and SDS + Triton X-100 were used as the detergent used for decellularization in this experiment. The contents were compared according to the decellularization time (0, 12, 24, 48 and 72 hours), and the concentrations of SDS (0.2, 0.5, 0.7 and 1.0%) were mixed in 1.0% Triton X-100 to analyze the contents. When decellularized using SDS and Triton X-100, respectively, it was confirmed that the contents of DNA and GAGs were opposite to each other. And decellularization treatment for 24 hours at 0.5% SDS was able to obtain an effective decellular support. If decellularization studies of various detergents can be obtained an effective decellular support, and furthermore, cell culture experiments can confirm the effect on the cells.

Visual Analogue Scale을 사용하여 분석한 전침시행 요통환자의 통증 호전에 대한 임상 연구

손지형,임호제,이승현,한승혜,문성일,Son, Chi-hyoung,Lim, Ho-jea,Lee, Seung-hyun,Han, Seung-hye,Moon, Sung-il 대한침구의학회 2004 대한침구의학회지 Vol.21 No.5

Objective : To evaluate the pain improvement of the lumbago patients taken the electro-acupuncture therapy using Visual Analogue Scale. Methods : The 25 patients of 46 lumbago patients visited department of Acupuncture & Moxibustion in National Medical Center from 1st March to 20th August had taken the electro-acupucture therapy more than 5 times. We checked on their age, sex, onset, radiating pain, back pain past history and Straight Leg Raising test. And we evaluated their improvement of back pain using Visual Analog Scale(VAS) at each visit. Then we analyzed the pain improvement by their age, sex, onset, radiating pain, back pain past history, Straight Leg Raising test, visiting frequency and Visual Analog Scale on 1st visit(VAS1) Results : VAS of 24 patients has improved and VAS of one patient has not been changed. There's no patient getting worse. The women and the positive group at SLR Test had better result of pain improvement. Conclusion : The lumbago patients taken electro-acupuncture more than 5 times showed significantly different pattern of pain improvement according to the sex and the SLR test.

The Assessment of Reporting Interventions in Randomized Controlled Trials of Acupuncture according to the STRICTA Recommendation

손지형,조성일,박히준,문성일,Son, Chi-Hyoung,Cho, Sung-Il,Park, Hi-Joon,Moon, Sung-Il 경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.2

목 적 : Pubmed에서 검색된 침의 효과에 대한 무작위 대조군 임상시험논문에서 STRICTA 권고안의 반영률을 알아보고자 본 연구를 계획하였다 . 방 법 : Pubmed에서 2003 년 7 월 1 일부터 2004 년 6 월 30 일까지 발간된 침 무작위 대조군 임상시험 논문을 검색 한 후 수작업으로 침의 효과에 대해 무작위 대조군 임상시험을 실시한 36 개의 논문을 채택하였다 .대상논문들의 저널, 대상 질병에 대해 조사하였으며 Jadad scale 접수를 매기고 STRICT A 권고안의 반영률을 ‘STRICTA 접수’를 매겨서 알아보았다. 논문이 SCI, SCIE, 무등재된 저널 중 어디에 실려 있는가에 따라 ANOVA 를 사용하여 Jadad 점 수 및 STRICTA 점수에 차이가 있는지 알아보았으며 단순상관 분석을 통해 STIRCT A 점수와 Jadad scale 접수의 관계를 살펴보았다. 결 과 : Jadad scale 접수의 평균은 2.6점 이었으며 STRICTA 접수의 평균은 11.0점 이었다. 36편의 침 무작위 임상시험논문에서 다음의 6가지 항목 - 문현적 근거, 자침 깊이, 함께 처치한 내용, 시술자 배경, 참가자의 맹검여부. 대조군 선택을 정당화 할 수 있는 근거-의 기술율은 50% 미만이었다. SCI, SOE, 무둥재된 것에 따라 논문들의 Jadad 접수 평균은 통계적으로 유의한 차이를 보였으나 STRICTA 점수 평균에서는 유의한 차이를 보이지 않았다. 한편 STRICTA 점수와 Jadad scale 점수 간에는 아무런 관계가 없었다. 결 론 :36 편의 침 임상시험 논문은 STRICTA 권고안의 많은 부분을 받아들이고 있었으며 STRICTA 권고안을 받아들이는 정도는 Jadad 점수와는상관이 없었다.

만성폐쇄성폐질환의 급성 악화로 응급실에 내원한 환자에서 DECAF 점수의 유용성

손지형,이장영,양영모,성원영,서상원,김진철,이원석 대한중환자의학회 2013 Acute and Critical Care Vol.28 No.4

Background: Exacerbations of chronic obstructive pulmonary disease (COPD) are common and can be fatal. However, it is difficult to predict the in-hospital mortality, severity and prognosis of patients. Prognostic tools are needed to assess exacerbations of COPD in the emergency department. Towards this end, we compared DECAF (dyspnea, eosinopenia, consolidation, acidemia, atrial fibrillation)score with other prognostic tools available in the emergency department. Methods: Consecutive patients admitted to the emergency department with exacerbations of COPD were recruited. We compared the DECAF score to CAPS (chronic obstructive pulmonary disease and asthma physiology score), BAP (blood urea nitrogen, altered mental status, pulse)-65 class and CURB (confusion, urea, respiratory rate, blood pressure)-65 score and assessed in-hospital mortality, endotracheal intubation, admission to the intensive care unit and admission to the hospital. Results: The in-hospital mortality rate was 4.9%. The DECAF score showed excellent discrimination for in-hospital mortality (AUROC = 0.72, p = 0.002), endotracheal intubation (AUROC = 0.92, p < 0.001), admission to the intensive care unit (AUROC = 0.90, p <0.001) and admission to the hospital (AUROC = 0.83, p < 0.001). Conclusions: The DECAF score is a simple and effective prognostic tool for assessing cases involving exacerbation of COPD in the emergency department. Emergency physicians should consider hospital admission if the DECAF score is more than 1 and consider admission to the intensive care unit and endotracheal intubation if the DECAF score is more than 3.

Visual Analog Scale을 사용하여 분석한 특발성 동결견 환자의 경부 통증 감소에 대한 전침의 효과 연구

손지형,임호제,이원희,정병주,한승혜,성수민,문성일 대한침구의학회 2005 대한침구의학회지 Vol.22 No.4

Objectives : To evaluate the pain improvement of the frozen shoulder patients taken the electro-acupuncture therapy using Visual Analogue Scale. Methods : The 38 frozen shoulder patients visited department of Acupuncture & Moxibustion in National Medical Center from 1st September 2004 to 28th February 2005 had taken the electroacupuncture therapy or body-acupuncture therapy more than 3 times.(Electroacupuncture group was 24 persons and body-acupuncture group was 14 persons.) We evaluated their improvement of shoulder pain using Visual Analog Scale(VAS) at each visit. Then we analyzed the pain improvement comparing with two groups. Results : Both VAS scores of electroacupuncture group and body-acupuncture group have significantly improved. VAS score at third visit of electroacupuncture group was significantly lower than that of body-acupuncture group (p=0.0057). The decreased VAS score after first visit of electroacupuncture group was also significantly higher than that of body-acupuncture group(p=0.0225). Conclusion : The pain of electroacupuncture group decreased more than body-acupuncture group at early visit. But the total decreased VAS scores of two groups was not significantly different.

뇌성마비 환아 한의치료 기술에 대한 전문가 설문 및 심층 면접 연구

손지형,윤영주,유선애,Son, Chi Hyoung,Yun, Young Ju,Yu, Sun Ae 대한한방소아과학회 2016 대한한방소아과학회지 Vol.30 No.4

Objectives This study aims to investigate current status of the Korean medicine treatment on the cerebral palsy (CP) via interviewing the doctors who have expertise in CP children. Methods From June 2015 to May 2016, the surveys and in-depth interviews were conducted on the fifteen Korean medicine doctors. Results Based on the surveys and interviews, widely-used treatments were body acupuncture and herbal decoction. Scalp acupuncture, intradermal acupuncture and manipulation were also used frequently. Experts stated Korean medicine could be more effective in younger patients, patients with cognitive or language problem. They also stated that Korean medicine has its advantage on strengthening the body to help grow and develop in CP children. However, the absence of guideline related to Korean medicine treatment in CP children as well as high cost are the problems. Finally, the issues regarding Korean medicine treatment in CP children, based on the in-depth interviews, emerged when western medicine is used simultaneously, when having seizure during treatment. Further research is needed for better understanding. Conclusions This study provided current status of the evidence-based Korean medicine treatment in CP children. However, further studies about the addressed issues are needed.

침수 및 온도변화에 따른 레진브라켓 Wing의 파절강도 비교

손지형,황현식 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

The purpose of this study was to evaluate the effect of oral environment on the strength of resin bracket wings by comparing fracture mode according to water immersion and thermal change. Seventy-five resin brackets(Spirit MB, Ormco, California) were divided into three groups and treated for six months as follows; (1) untreated, (2) water immersion in distilled water at 37℃, (3) water immersion in distilled water at 37℃ with total 2,100 times of thermocycling taken 350 times each month. Fracture mode of the wing was tested on universal testing machine. In addition to resin brackets, 25 metal brackets were used as controls. Through the statistical analyses, following results were obtained. 1. Resin bracket wings showed significantly lower fracture strength than metal brackets(p<0.001). 2. Water immersion and water immersion with thermocycling groups showed significantly lower fracture strength than open air condition group(p<0.001). 3. Water immersion with thermocycling group showed significantly lower fracture strength than water immersion group(p<0.001> The above results suggest that the mechanical property of resin bracket wing may be influenced by oral environment and further research is needed to improve the strength of the wing in the resin bracket.

침수 및 열순환에 따른 레진브라켓 Wing의 파절강도 변화

손지형,황현식 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.4

본 연구는 침수 및 온도변화에 따른 레진 브라켓 wing의 파절양상을 비교하여 구강내 환경이 레진 브라켓 wing의 물성변화에 미치는 영향을 관찰해 보기 위하여 시행되었다. 본 연구의 재료로 레진 브라켓 75개와 금속 브라켓 25개를 사용하였는데, 레진 브라켓은 상온에서 6개월 동안 방치한 경우와 37℃의 증류수에 6개월 동안 침수시킨 경우, 그리고 37℃의 증류수에 6개월 동안 침수시킨 상태에서 1개월에 350회씩 총 2,100회의 열순환 시킨 경우의 3가지 군으로 구분하여 처리하였다. 만능물성 시험기를 이용하여 금속 브라켓의 경우 wing의 변형강도를, 레진 브라켓의 경우 파절강도를 각 군별로 측정하고 비교 분석하여 다음과 같은 결과를 얻었다. 1.금속 브라켓 wing의 변형강도에 비하여 레진 브라켓 wing의 파절강도가 매우 낮게 나타났으며 통계적으로 유의한 차이가 있었다(p〈0.001). 2.상온에서 6개월 동안 방치한 군에 비하여 침수시키거나 침수 및 열순환 시킨 군에서 브라켓 wing의 파절강도가 낮게 나타났으며 통계적으로 유의한 차이를 보였다(p〈0.001). 3.단순침수에 비하여 침수 및 열순환 시킨 군의 브라켓 wing의 파절강도가 더 낮게 나타났으며 통계적으로 유의한 차이가 있었다(p〈0.001). 이상의 결과는 구강내 환경에 의하여 레진 브라켓 wing의 물성이 약화되므로 이의 강도증가를 위한 적절한 대책이 필요함을 시사하였다. The purpose of this study was to evaluate the effect of oral environment on the strength of resin bracket wings by comparing fracture mode according to water immersion and thermocycling. Seventy-five resin brackets(Spirit MB, Ormco, California) were divided into three groups and treated for six months as follows, (1) untreated, (2) water immersion in distilled water at 37℃, (3) water immersion in distilled water at 37℃ with total 2,100 times of thermocycling taken 350 times each month. Fracture mode of the wing was tested on universal testing machine. In addition to resin brackets, 25 metal brackets were used as controls. Through the statistical analyses, following results were obtained. 1.Resin bracket wings showed significantly lower fracture strength than metal brackets(p〈0.001). 2.Water immersion and water immersion with thermocycling groups showed significantly lower fracture strength than open air condition group(p〈0.001). 3.Water immersion with thermocycling group showed significantly lower fracture strength than water immersion group(p〈0.001). The above results suggest that the mechanical property of resin bracket wing may be influenced by oral environment and further research is needed to improve the strength of the wing in the resin bracket.

Effects of Three Thiazolidinediones on Metabolic Regulation and Cold-Induced Thermogenesis

손지형,김종인,전용근,박제우,김재범 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.10

Insulin resistance is closely associated with metabolic diseases such as type 2 diabetes, dyslipidemia, hypertension and atherosclerosis. Thiazolidinediones (TZDs) have been developed to ameliorate insulin resistance by activation of peroxisome proliferator-activated receptor (PPAR) γ. Although TZDs are synthetic ligands for PPARγ, metabolic outcomes of each TZD are different. Moreover, there are lack of head-to-head comparative studies among TZDs in the aspect of metabolic outcomes. In this study, we analyzed the effects of three TZDs, including lobeglitazone (Lobe), rosiglitazone (Rosi), and pioglitazone (Pio) on metabolic and thermogenic regulation. In adipocytes, Lobe more potently stimulated adipogenesis and insulin-dependent glucose uptake than Rosi and Pio. In the presence of pro-inflammatory stimuli, Lobe efficiently suppressed expressions of pro-inflammatory genes in macrophages and adipocytes. In obese and diabetic db/db mice, Lobe effectively promoted insulin-stimulated glucose uptake and suppressed pro-inflammatory responses in epididymal white adipose tissue (EAT), leading to improve glucose intolerance. Compared to other two TZDs, Lobe enhanced beige adipocyte formation and thermogenic gene expression in inguinal white adipose tissue (IAT) of lean mice, which would be attributable to cold-induced thermogenesis. Collectively, these comparison data suggest that Lobe could relieve insulin resistance and enhance thermogenesis at low-concentration conditions where Rosi and Pio are less effective.

‘미디어 관련법’ 보도의 뉴스 프레임 연구

손지형(Ji-Hyoung Son),하승태(Sung-Tae Ha),이범수(Bum-Soo Lee) 부산울산경남언론학회 2013 지역과 커뮤니케이션 Vol.17 No.2

보수와 진보 언론간의 갈등은 우리 언론의 객관성 및 공정성과 관련해 적지 않은 문제점들을 노정해 왔다. 이러한 갈등은 특히 이념적 성향이나 이해관계에 따라 언론사들의 첨예한 프레임 대결로 이어졌다. 본 연구는 언론사들과 직접적인 이해관계가 얽힌 ‘미디어 관련법’에 대한 언론사별 뉴스보도의 비교분석을 통해 이념적 성향에 따라 관련 뉴스프레임이 어떤 차이를 보이는지 살펴보았다. 분석 결과, 미디어 관련법에 관한 보도량, 기사유형, 게재면, 정보원, 주요이슈에서 보수신문과 진보신문간 뚜렷한 차이를 보였다. 보도량에서는 진보신문이 보수신문에 비해 두 배 이상 많은 지면을 할애 했다. 정보원 이용을 살펴보면 진보신문은 노조와 시민단체, 보수신문은 정치권을 주요 정보원으로 활용한 것으로 나타났다. 주요 이슈에서는 보수신문은 산업 활성화 측면을, 진보신문은 언론장악 저지 측면을 주로 다루었다는 점에서 차이를 보였다. 프레임 분석결과, 우선 형식적 측면에서는 단편적인 사건을 다룬 일화적 프레임을 보수신문들 주로 사용한 반면 진보신문들은 관련 이슈의 원인과 배경을 다루는 주제적 프레임을 더 많이 사용한 것으로 관찰되었다. 내용적 측면에서는 갈등 프레임과 책임귀인 프레임이 보수 및 진보신문사 모두에서 주요 프레임을 분석되었다. 그러나 경제적 중요성의 경우 보수신문이 상대적으로 많이 다룬 반면 도덕성 프레임은 진보신문에서 현격히 많이 다룬 것으로 나타났다. 보수와 진보신문의 보도가 특정 프레임에 집중되어 있다는 점에서 각 신문사는 미디어 관련법 보도에 있어서 다양한 의견과 시각이 충분히 반영되지 않고 있는 것으로 풀이된다. Since the ‘media law’was suggested in December, 2008, the precipitated kinds of social and political conflict aroused the attention to the function about the reality definition of the suggestion. The conflict between the conservative and liberal media serve as a momentum of a huge suspicion on the objectiveness and fairness of the media. As the result of this study, in the qualitative characteristics, the aspect of report quantity, article type, insertion page, information resources, major issue was widely different. In the report quantity, liberal newspapers reported more than twice as conservative newspaper did. Concerning the information sources, it was noticeable that the liberal newspapers relied more on labor union and civil association while the conservative newspapers on political parties. Episodic frames appeared more frequently than thematic frames in the conservative newspapers whereas vice-versa in the liberal one. As the conservative and liberal newspapers focused on the specific frames, it was grasped that each press did not cover the various perspectives of the media law issues in their reporting.