홍삼 생약 복합물(KTNG0345)이 섬유아세포의 Procollagen 생합성과 Matrix metalloproteinase-1(MMP-1) 활성에 미치는 영향

소승호,이성계,황의일,구본석,한경호,김나미,So, Seung-Ho,Lee, Seong-Kye,Hwang, Eui-Il,Koo, Bon-Suk,Han, Gyeong-Ho,Kim, Na-Mi 고려인삼학회 2007 Journal of Ginseng Research Vol.31 No.4

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3 (KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 $1{\sim}10\;{\mu}g/ml$의 농도로 첨가하여 세포를 배양했을 때 $83{\sim}100%$의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ug/ml의 농도를 기준으로 할 때 홍삼은 533.9ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68:232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136:164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ug/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ug/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblas에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136:164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3 (KTNG0345)는 지표성분으로서 ginsenoside-$Rb_1$, torilin, loganin을 각각 10.85 mg/g, 0.128 mg/g, 3.92 mg/g 함유하였다. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-$Rb_1$, torilin and loganin were identified at 10.85 mg/g, 0.128 mg/g and 3.92 mg/g respectively.

홍삼 생약 복합물(KTNG0345)이 섬유아세포의 Procollagen 생합성과 Matrix metalloproteinase-1(MMP-1) 활성에 미치는 영향

소승호(Seung-Ho So),이성계(Seong-Kye Lee),황의일(Eui-Il Hwang),구본석(Bon-Suk Koo),한경호(Gyeong-Ho Han),김나미(Na-Mi Kim) 고려인삼학회 2007 Journal of Ginseng Research Vol.31 No.3

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3(KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 1~10 ㎍/ml의 농도로 첨가하여 세포를 배양했을 때 83~100%의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ㎍/ml의 농도를 기준으로 할 때 홍삼은 533.9 ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68 : 232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136 : 164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ㎍/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ㎍/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblast에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136 : 164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3(KTNG0345)는 지표성분으로서 ginsenoside-Rb1, torilin, loganin을 각각 10.85 ㎎/g, 0.128 ㎎/g, 3.92 ㎎/g 함유하였다. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-Rb₁, torilin and loganin were identified at 10.85 ㎎/g, 0.128 ㎎/g and 3.92 ㎎/g respectively.

홍삼 생약 복합물(KTNG0345)의 피부 주름개선에 관한 작용기전

소승호(Seung-Ho So),이성계(Seong-Kye Lee),황의일(Eui-Il Hwang),구본석(Bon-Suk Koo),한경호(Gyeong-Ho Han),정진호(Jin Ho Chung),이민정(Min-Jung Lee),김나미(Na-Mi Kim) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1

본 실험은 홍삼 혼합물 (KTNG0345)을 이용한 주름 예방 및 개선효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 시료를 경구투여한 무모생쥐의 피부조직으로부터 MMP-3의 발현양상과 작용 메커니즘을 연구하였다. MMP-3의 발현정도는 농도 의존적으로 현저한 감소를 나타내었으며, 유전자와 단백질 모두에서 동일한 양상을 보였다. PAK는 변화가 없었지만, p38, p-p38 그리고 c-Jun, p-c-Jun을 통계적으로 유의하게 감소시킴으로써 MMPs의 발현 감소를 가져온 것으로 보인다. 뿐만 아니라 자외선에 의한 TNF-α의 생성 또는 유입을 억제함으로써 TNF-α receptor에 의해 매개되는 신호전달 경로를 둔화시켜 MMPs의 발현을 감소시킨 것으로 보인다. 이렇게 KTNG0345는 복합적인 활성으로 작용하여 주름생성 억제 활성을 보이는 것으로 판단된다. UV irradiation causes skin-aging involving coarse wrinkles, thickening, dyspigmentation, and rough skin surface. These phenomena in complex skin tissue is controlled with receptor of cell surface growth factor and cytokine receptors. The activation of receptors induces multiple downstream signaling pathways including expression of MMPs (matrix metalloproteinases). This study was aimed to elucidate the mechanism for anti-wrinkle activity of Korean red ginseng, Torilis fructus and Corni fructus mixture (KTNG0345). In this animal study, we have investigated decreasing effects of Korean red ginseng mixture on MMP-3 synthesis through diminishing TNF-α signaling that express MMP-1, -3, and -9. c-Jun and c-fos as a component of transcription factor AP-1 (activator protein-1) were analyzed the expression level using real time PCR and western blotting. c-Jun was decreased dose dependent manner both gene and protein level where as cfos was not changed. In upstream, JNK and PAK was not changed, but p38 was decreased in down stream. MMP-3, final product in this pathway was significantly decreased in dose dependent manner. These results suggest that Korean red ginseng mixture have a anti-wrinkle activity through TNF-α mediated MMPs expression pathway.

The world ginseng market and the ginseng (Korea)

백인호,소승호 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.1

Ginseng is being distributed in 35 countries around the world and there are differences by each country in the distributionvolume and amount. However, since there is no accurate statistics on production and distribution amount by each country, it is verydifficult to predict the world ginseng market. Ginseng trading companies and governments are in desperate need of comprehensivedata that shows the world ginseng market status for sales and marketing. For that reason, this study will look into the approximatesize of the world ginseng market based on recent ginseng distribution amount by each country and production by major ginsengproducing nations. In addition, the review sets an opportunity to check the status of ginseng (Korea) in the world and presentsfuture direction by examining recent history of ginseng development in Korea, which is one of the world’s largest ginsengdistributers. Since ginseng is cultivated in limited areas due to its growth characteristics, ginseng distributing countries can bedivided based on whether they grow it domestically or not. In general, four countries including South Korea, China, Canada, andthe US are the biggest producers and their total production of fresh ginseng is approximately 79,769 tons which is more than 99%of 80,080 tons, the total ginseng production around the world. Ginseng is distributed to different countries in various forms such asfresh ginseng, dried ginseng, boiled and dried ginseng (Taekuksam), red ginseng and the related products, etc. and is consumed asfood, dietary supplements, functional food, medical supplies, etc. Also, the world ginseng market including ginseng root and theprocessed products, is estimated to be worth $2,084 million. In particular, the size of the Korean market is $1,140 million whichmakes Korea the largest distributer in the world. Since the interests in alternative medicine and healthy food is increasing globally,the consumer market of ginseng with many features and the processed products are expected to expand continuously.

고지방식이로 유도된 비만 마우스의 체중 감소에 두충우슬복합물(KGC08EA)이 미치는 영향

권한올,소승호,심성례,김종한,장경화,서재현,성준엽,정윤선,이정선,이승호 한국식품영양과학회 2023 한국식품영양과학회지 Vol.52 No.11

본 연구에서는 두충과 우슬의 추출복합물(KGC08EA)이 체내 에너지대사와 지방대사와의 연관성, 그리고 그에 따른 체지방 변화에 미치는 영향을 확인하기 위하여 고지방식이로 유도된 비만 동물 모델에서 DXA 측정을 통한 체성분, 혈중 아디포넥틴 및 렙틴, 간 조직의 에너지대사 조절 단백질, 지방조직의 지방대사 조절 단백질의 변화를 살펴보았다. 그 결과 체중 및 총지방량의 감소와 함께 지방의 합성과 분해 및 축적은 식욕 및 염증과 관련된 아디포넥틴 및 렙틴과 에너지 대사와 관련된 p-AMPK 및 GLP-1R, 그리고 지방과 관련 세포 분화, 지방합성, 산화 및 분해와 관련된 PPAR-γ, HSL, ACC의 조절을 통해 항비만 효과를 나타내는 것을 확인하였다. 따라서 KGC08EA는 고지방식이로 유도된 비만동물 모델에서 에너지 대사 조절을 통한 항비만 효과와 함께 간과 신장의 안전성 관련 지표 확인을 통해 안전한 소재임을 확인하였다. In this study, a combination of Eucommia ulmoides and Achyranthes japonica (KGC08EA) demonstrated anti-obesity effects related to energy and lipid metabolism in vivo. To confirm their effectiveness in animals, high-fat diet (HFD) induced C57BL/6J mice were orally administrated KGC08EA for 9 weeks. The experimental groups were as follows: NC (C57BL/6J on a normal diet), C (HFD), PC (Coleus forskohlii, 100 mg/kg b.w.), EA100 (KGC08EA, 100 mg/kg b.w.), EA200 (KGC08EA, 200 mg/kg b.w.), EA400 (KGC08EA, 400 mg/kg b.w.), and EA800 (KGC08EA, 800 mg/kg b.w.). KGC08EA significantly decreased body weight, total fat and serum leptin, alanine aminotransferase, triglycerides, total cholesterol, and low density lipoprotein but significantly increased serum adiponectin. KGC08EA enhanced levels of energy and lipid metabolism modulators, such as phosphorylated adenosine monophosphate activated protein kinase, glucagon-like peptied 1 receptor, peroxisome proliferator-activated receptor gamma, acetyl-CoA carboxylase, and hormone-sensitive lipase, in liver and epididymal fat. These results suggest that KGC08EA has potential use as a health food for obesity.

사상자(Torilis Fructus)가 섬유아세포의 Procollagen 생합성과Matrix metalloproteinase-I(MMP-1)의 활성에 미치는 영향

구본석,황의일,소승호,이성계,한경호,김나미 한국생약학회 2007 생약학회지 Vol.38 No.4

wrinkle formations are associated with collagen synthesis and matrix metalloproteinase-I(MMP-1) activity.70% ethanol and the ethanol extracts were systematically fractionated with n-hexane, ethylacetate, n-butanol and distilled water.Among them, antiwrinkle component from n-hexane fraction was purified by several column chromatographies and HPLC,which identified as torilin by 1H-NMR, 13C-NMR and ESI -MS. To determine cell viability, collagen biosynthesis and MMP-1 activity, human dermal fibroblast was treated with 1-5 ppm concentrations of Torilis Fructus extract fraction and torilin. Cellviability was showed 84-102% at all group treated with 1-5 ppm. Collagen synthesis was increased in all group, especially tori-lin-treated group was highest amount. Active forms of MMP-1 were decreased in all group. From these results, we considerthat Torilis Fructus have several antiwrinkle components and torilin may be one of the efective components.Key words ... (Torilis Fructus). ... (Umbelliferae). ..... (Totilis japonica). .... ...... ......... ... , ... , .... , .... , ... ...... ... .. .1)..... .. , .. , ... , ..... , ....... , .... .. ..... , .... .. .. , ... .. .. ... .. ... ...... .2,3,4,5)

사상자 (Torilis Fructus)의 지표성분으로 Torilin의 분리 및함량분석법 개발

황의일,이성계,소승호,구본석,한경호,김나미 한국생약학회 2007 생약학회지 Vol.38 No.4

the propose of the quality control of Torilis Fructus, one major compound was isolated by several column chro-analysis of torilin using HPLC method showed that the average contents at 230 and 254 nm were 7.167±0.168 mg/g and 7.052±0.160 mg/g, respectively, in 18 samples collected throughout the regions of Korea and China. This method provided a toolfor standardization of the comercial Torilis Fructus.Key wordsTorilis Fructus, Quantitative analysis, Torilin, HPLC... (Torilis Japonica Decandolle). ... ....... , .... ... ... .... ... (Um-belliferae). ... .. 60 cm ... ... .... .. .... .... . .. (Torilis Fructus). .... ... 2.. ... ... .. ... ....... .. 3~5m, . 1.5 m .. , ... .... .... .

Subacute oral toxicity and bacterial mutagenicity study of Korean Red Ginseng oil

서휘원,서재현,소승호,경종수,김용순,한창균 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.4

Background: Red ginseng oil (RGO) is produced by supercritical CO2 extraction of secondary products derived from Korean Red Ginseng extract. As the use of RGO has increased, product safety concerns have become more important. Methods: In the present study, the subacute oral toxicity and bacterial reverse mutagenicity of RGO were evaluated. SpragueeDawley rats were orally administered with RGO for 28 d by gavage. Daily RGO dose concentrations were 0 mg/kg body weight (bw), 500 mg/kg bw, 1,000 mg/kg bw, or 2,000 mg/kg bw per day. Bacterial reverse mutation tests included five bacterial strains (Escherichia coli WP2 and Salmonella typhimurium TA98, TA100, TA1535, and TA1537), which were used in the presence or absence of metabolic activation. The plated incorporation method for mutation test was used with RGO concentrations ranging from 312.5 mg to 5,000 mg per plate. Results: The subacute oral toxicity test results did not reveal any marked changes in clinical characteristics. There were no toxicological changes related to RGO administration in hematological and serum biochemical characteristics in either control or treatment animals. Furthermore, no gross or histopathological changes related to RGO treatment were observed. The bacterial reverse mutation test results did not reveal, at any RGO concentration level and in all bacterial strains, any increase in the number of revertant colonies in the RGO treatment group compared to that in the negative control group. Conclusion: The no-observed-adverse-effect level of RGO is greater than 2,000 mg/kg bw and RGO did not induce genotoxicity related to bacterial reverse mutations.

Subacute Oral Toxicity and Bacterial Mutagenicity Study of a Mixture of Korean Red Ginseng (Panax ginseng C.A. Meyer) and Salvia plebeia R. Br. Extracts

서휘원,서재현,경종수,장경화,소승호 한국독성학회 2019 Toxicological Research Vol.35 No.3

As various populations are rapidly becoming an aging society worldwide and interest in health issues has increased, demand for functional foods including herbal products has increased markedly to maintain a healthy state which has led to safety issues about their intake as an inevitable result. The objective of this study was to identify the safety profile of a Korean red ginseng and Salvia plebeia R. Br. extract mixture (KGC-03-PS) which is a valuable ingredient that can be used as a functional food. In the present study, the subacute oral toxicity and bacterial reverse mutagenicity of KGC-03-PS were evaluated. Sprague Dawley rats were administered KGC-03- PS orally for 28 days by gavage. Daily KGC-03-PS dose concentrations were 0, 500, 1,000, or 2,000 mg/kg body weight (bw) per day. Bacterial reverse mutation test with KGC-03-PS dose levels ranging from 312.5 to 5,000 μg/ plate was carried out by OECD test guideline No. 471. Five bacterial strains (Salmonella typhimurium TA98, TA100, TA1535, TA1537, and Escherichia coli WP2) were tested in the presence or absence of metabolic activation by plate incorporation method. There were no toxicological effects related with test substance in the clinical evaluation of subacute oral toxicity test including clinical signs, body weight, and food consumption. Moreover, no toxicological changes related to KGC-03-PS were observed in the hematological and serum biochemical characteristics as well as in the pathological examinations, which included organ weight measurements and in the gross- or histopathological findings. KGC-03-PS did not induce an increase in the number of revertant colonies in all bacterial strains of the bacterial reverse mutation test. The no-observed-adverse-effect level of KGC-03-PS is greater than 2,000 mg/kg bw/day, and KGC-03-PS did not induce genotoxicity related to bacterial reverse mutations under the conditions used in this study.

홍삼 및 천연물 혼합소재의 비만 유도 흰쥐에 대한 항비만 효과에 관한 연구

이성계(Seongkye Lee),소승호(Seungho So),황의일(Euiil Hwang),구본석(Bonsuk Koo),한경호(Gyeongho Han),고상범(Sangbeom Ko),김나미(Nami Kim) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.4

홍삼과 보이차, 노팔선인장을 소재로 하여 체중조절효과가 우수하고 부작용이 없는 최적의 원료와 적정 섭취 농도를 설정하고자 홍삼, 보이차, 노팔선인장 혼합소재(DM1) 및 홍삼, 보이차 혼합소재(DM2)를 고지방식이로 8주간 비만을 유도한 수컷 흰쥐에게 100, 300, 500 ㎎/㎏의 농도로 8주간 투여하면서 체중, 혈중 지질 분석, 복부 및 기타지방 조직, 비만에 관련된 몇 가지 생화학적 지표를 조사하였고, 간 기능과 신장 기능, 조혈 기능을 나타내는 생화학적 지표를 조사하였다. 체중은 고지방식이군에 비하여 DM1 투여군에서 77.1~51.8%, DM2 투여군에서 64.7~16.1% 수준으로 증가하였다. 중성 지질의 농도는 DM1 투여군에서 고지방 식이군의 84~54%, DM2 투여군에서 62~58%의 수준을 나타내었다. 혈청 렙틴의 농도는 DM2 투여군에서 농도 의존적으로 감소하여 고지방식이군의 82.8~66.9%로 감소되었다. 혈중 인슐린의 농도는 DM1 투여군에서 고지방식이군의 66.8~65.1%, DM2 투여군에서 66.7~62.5%의 수준이었으며 1% 수준에서 통계적인 유의성을 나타내었다. 부고환 주위지방(좌/우), 내장지방, 신장주위 지방의 무게는 DM1 500 ㎎/㎏과 DM2 300, 500 ㎎/㎏ 투여에서 1% 수준에서 통계적으로 유의적인 감소를 나타내었고 DM2는 정상식이군의 수준으로까지 감소되는 우수한 효과를 나타내었다. 간 조직내 지질침착도는 DM2 500 ㎎/㎏ 투여군에서는 감소하였다(p<0.05). 간과 신장의 이상 유무를 판단할 수 있는 AST, ALT 및 ALP, T-BIL, BUN 및 WBC 수치는 시험물질 섭취군에서 고지방식이군에 비하여 큰 변화를 나타내지 않았고 RBC는 DM2 500 ㎎/㎏ 투여군에서 다소 증가하였고 다른 시험군에서는 큰 변화가 없어서 부작용이 없음을 확인하였다. 이상의 실험결과를 종합해 보면 홍삼과 보이차, 노팔선인장을 소재로 한 DM1과 DM2 복합 소재는 항비만 효과가 우수하고 부작용이 없었으며, 홍삼과 보이차를 혼합한 DM2의 효과가 더 우수하였으며, 적정 섭취 농도는 DM1은 500 ㎎/㎏, DM2는 300~500 ㎎/㎏인 것으로 판단된다. Anti-obesity effects of ginseng and herbal plant mixtures were investigated to develop natural materials for anti-obesity. After inducing obesity with high fat diet for 8 weeks in male SD rats, ginseng and herbal plant mixtures DM1 (ginseng, puer tea, opuntia) and DM2 (ginseng, puer tea) were administrated orally to rats for another 8 weeks. During administration, food efficacy ratio and body weight of rat were measured twice weekly. After administration, body weight, body fat contents, and serum lipid level were estimated for anti-obesity effect and hematological analysis blood level of ALP and ASP was checked for safety. Body weight in rats fed high fat diet was significantly increased. Body weight in obese rats induced by high fat diet was significantly decreased by DM1 and DM2 feedings. The amount of body fat (epididymal, perirenal and visceral fat, brown adipose tissue) was significantly reduced by DM1 and DM2 treatments. The amount of TG, the concentration of leptin in blood plasma, and the concentration of insulin in blood plasma were significantly diminished by DM1 and DM2. Lipid accumulation on liver was reduced in DM2. There were no side effects among all groups according to blood analysis, hematological findings, and body weight. The findings of this study suggest that DM1 and DM2 may be effective materials for anti-obesity through reducing plasma triglyceride and body fats, and also decreasing body weight without side effects.