가미보아탕(加味保我湯) 및 수종(數種) 한방처방의 항암효과에 대한 연구

성현제,Sung, Hyun-Jea 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.2

Objectives : Anticancer and immune-modulating effects of several Korean medical prescriptions including Yukgunja-tang, Bohwa-tang, Sogam-Won, and Kamiboa-tang were investigated. Methods : In vitro anti-cancer effects were measured by cytotoxicity MTT assay using SNU-1 gastric cancer cell lines, In vivo anti-cancer effects were measured by increased life span of S-180 sarcoma-injected ICR mouse. Immune-modulating effects were analyzed by measuring hemagglutinin titer, appearance of rosette forming cells, lymphocyte proliferation, and phagocytic index in methotrexate-pretreated mice. Results : In vitro assay showed that only Sogam-won showed cytotoxic effect with $IC_{50}$ of 87.9 ${\mu}g/ml$. All other prescriptions showed no cytotoxic effects against SNU-1 gastric cancer cell line. However, in vivo assay showed that Sogam-won showed lowest anti-cancer effects in contrast to its highest cytotoxic effects, Kamiboa-tang, which showed no cytotoxic effect, showed the highest in vivo anticancer effects, with increased life span of 140%. Kamiboa-tang showed significant immune-enhancing activities by significantly increasing rosette forming cells, lymphocyte proliferation, and phagocytic index in methotrexate-pretreated mice (P<0.05). Conclusion : The anticancer effect of Kamiboa-tang is not mediated by direct inhibition of cancer cells but is mediated by improving immune reactions against cancer cells.

홍삼(紅蔘), 황기, 감국(甘菊) 혼합물(混合物) 제재 효과에 관한 연구

성현제,정종운,류충열,허억,Sung Hyun-Jea,Chung Chong-Un,Ryu Choong-Yul,Her Erk 대한한의학방제학회 2004 大韓韓醫學方劑學會誌 Vol.12 No.1

1. All the male students and female students who had taken these herb medicines showed a strong increase in immunity (P<0.01) and they were more effective to the male students (P<0.01) rather than the female students (P<0.05) (figure 1). Generally, immunity may be reduced at the time of fatigue and overwork but as these herb medicines have the effect of increasing immunity. Therefore, it is considered that they not only reduce the fatigue of human body but also heighten immunity against disease. 2. The students who had taken these herb medicines showed some reduction in Cortisol concentration in blood, which didn't show the difference of concentration up to statistical meaning compared to before taking the medicines (figure 2). Accordingly, it is regarded that these herb medicine drink restrained stress slightly. 3. The students who had taken these herb medicines showed some reduction in BUN concentration in blood, which didn't show the difference of concentration up to statistical meaning compared to before taking the medicines (figure 3). For the women, they restrained the toxicity of the kidneys just a little. Accordingly, it shows that these medicines are safe herb medicines without the toxicity of the kidneys. 4. The students who had taken these herb medicines didn't show the difference of concentration up to statistical meaning in sFOT and sGPT concentration in blood compared to taking them (figure $4{\sim}5$). Accordingly, it is suggested that these medicines are safe stuffs without the toxity of the liver. 5. Seven members of the university students answered to the questionaire that they were pleasant while taking the medicines and didn't feel fatigue. They answered very positively that they had better effects than before taking the medicines in many things (Refer to the Questionaire). However, one male student and two female students of them complained of temporary headache and indigestion for 2 days after taking these herb medicines but they said they had been taking cold medicines due to cold symptom before taking the medicines. Accordingly, it turns out that such temporary symptom is not irrelevant to these medicines. In conclusion, it is considered that these herb medicines have the effects of increasing immunity, restraining fatigue and stress through this clinical study carried out in simple way and that they are valuable as herb medicines without the toxity of the liver and kidneys.

소편불통(小便不通)에 사용되는 처방의 약물구성에 관한 연구

성현제,Sung, Hyun-Jea 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.1

Objectives : The aim of this study is to analyze the prescription used for dysuria in herbal medication. Methods : Investigating the Oriental Medicine textbooks, I found the prescription used for dysuria. So I analyzed the herbal medication for frequency or compositon and classified it. Results : 1. The most used prescription on dysuria is Paljung-san. 2. The order of frequency of herb for treating dysuria is Moktong, Hwalseok, Gamcho, 3. The group of Yisusamseopyak is used for main prescription about 42%.

가미홍화탕의 단회 경구 투여 독성 연구

성현제,문근아,유충렬,윤유식,Sung, Hyun-Jea,Moon, Geun-Ah,Ryoo, Choong-Ryeol,Yoon, Yoo-Sik 한국한의학연구원 2003 한국한의학연구원논문집 Vol.9 No.2

Kami-honghwa-tang(KH-19) is a prescription for reducing the side effect of radiotherapy. In this study, safety of KH-19 was evaluated by GLP guideline of Korea Food and Drug Administration. In acute oral toxicity study on rat, transient inhibition of weight increase was shown, but change in general symptom was not detected. No dead animal was observed up to 5,000 mg/kg in both male and female animals. In acute oral toxicity study on Beagle dog, transient vomiting, diarrhea, anorexia, and weight reduction was observed. However, no dead animal was observed up to 2,000 mg/kg in both male and female animals.

미래 한의학의 중장기예측 연구 동향

신현규,성현제,Shin, Hyun-Kyoo,Sung, Hyun-Jea 한국한의학연구원 1996 한국 한의학연구소 논문집 Vol.2 No.1

Oriental medicine is a traditional medical are that has maintained its medical system only in Korea, Japan and China, nowadays, it is required to systematize the Oriental medicine modernly as well as prove remedical value of it for public welfare and hygiene, medical studies about geriatric disease and ageing are valued highly in researchs of future medical service which were excuted by Korea, Japan and Germay. consequently, future - estimating project of the Oriental medicine which keeps and accent on research datas that have a curative effect highly must be constructed. in the cause of this, effieient and systematic subject selection should be preceded to accomplish an advisable planning of the Oriental medicine.

KH-19의 5-flurouracil 부작용 억제 효력 및 기전 연구

양동식,성현제,윤유식,Yang, Dong-Sik,Sung, Hyun-Jea,Yoon, Yoo-Sik 한국한의학연구원 2003 한국한의학연구원논문집 Vol.9 No.1

The objective on this study was to investigate medicinal protection with KH-19, which was composed of 9 kinds of oriental herbs tonifying the blood, against side effect of chemotherapy in mice and its mechanism through microarray. Not only WBC (white blood cell), and PLT (platelet) as a hematopoiesis toxicity indicator but also spleen weight as an immune toxicity indicator was reduced significantly 7 day after. 5-flurouracil (FU) treatment. However, reduction of WBC, PLT, and spleen weight after 5-FU treatment was significantly recovered by KH-19. For mechanic study on KH-19 action, gene expression in mouse spleen treated 5-FU was compared with gene expression in mouse treated 5-FU and KH-19. The result indicates that 23 genes were increased. in expression level over 2 fold and 41 genes were decreased in expression level more than 2 fold at mouse spleen by KH-19 treatment. KH-19 mechanism may be complicated more than other drug which of mechanism were composed of single compound because KH-19 was composed of various compounds.

Cyclophosphamide로 Rat에 유도된 악성빈혈에 대한 숙지황의 증(蒸)수에 따른 치료효능에 관한 연구

하창수,성현제,지옥표,마진열,Ha, Chang-Su,Sung, Hyun-Jea,Zee, Ok-Pyo,Ma, Jin-Yeul 한국생약학회 2000 생약학회지 Vol.31 No.3

Rhizoma Rehmanniae Preparata is a Chinese herbal tonic. The hemopoietic effects of Rhizoma Rehmanniae Preparata(4-time steamed Jihwang, 4-time steamed double dose Jihwang, 8-time steamed Jihwang, 8-time steamed double dose Jihwang) were examined using in vitro rat(SD) model. Cyclophosphamide(150 mg/kg) was injected into experimental groups and control group to induce bone marrow suppression. Oral administration of Rhizoma Rehmanniae Preparata suppressed hormone levels of $T_4\;and\;T_3$. Reticulocyte count was increased by the bone marrow suppression.

소목 추출물의 세포독성 효과와 Topoisomerase I 억제 활성에 관한 연구

박갑주,김수영,마진열,성현제,전원경,Park, Kap-Joo,Kim, Soo-Young,Ma, Jin-Yeul,Sung, Hyun-Jea,Jeon, Won-Kyung 한국생약학회 1999 생약학회지 Vol.30 No.1

To evaluate cytotoxic effect and topoisomerase I inhibition activity of Caesalpinia sappan L., both water and methanol extracts were examined using in vitro assay. The cytotoxic effect of Caesalpinia sappan L. examined using MTT and SRB assay and $IC_{50}$ values were measured against U937, HL60, HepG2, SNU-1, SNU-16 cancer cell lines. Among them the representative cytotoxic results are shown as follows; water extract (U937=13.39 ${\mu}g/ml$, HL60=8.65 ${\mu}g/ml$, HepG2=38.48 ${\mu}g/ml$, SNU-1=7.72 ${\mu}g/ml$, SNU-16=25.49 ${\mu}g/ml$), methanol extract (U937=13.35 ${\mu}g/ml$, HL60=9.43 ${\mu}g/ml$, HepG2=25.67 ${\mu}g/ml$, SNU-1=8.37 ${\mu}g/ml$, SNU-16=28.64 ${\mu}g/ml$). The inhibitory concentration of DNA topoisomerase I activity against water extract was 100 ${\mu}g/ml$ and the inhibitory concentration of DNA topoisomerase I against methanol extract was 400 ${\mu}g/ml$.

노화촉진 마우스의 Protein Carboxyl O-Methyltransferase 활성도 및 에너지대사에 대한 연구

마진열(Jin-Yeul Ma),성현제(Hyun-Jea Sung),정규용(Kyu-Yong Jung),홍성렬(Sung-Youl Hong) 한국실험동물학회 1999 Laboratory Animal Research Vol.15 No.1

사상체질분류검사에 의한 체질 진단과 체성분분석과의 상관성에 관한 연구

최선미,지상은,정봉연,성현제,안규석,고병희,Choi, Sun-mi,Chi, Sang-en,Jung, Bong-yeon,Sung, Hyun-jea,Ahn, Kyoo-seok,Koh, Byung-hee 사상체질의학회 2001 사상체질의학회지 Vol.13 No.1

Objectives : The purpose of this study was to evaluate the body shape of Sasang Constitutions by body composition and body measurement. Methods : This study was carried out 98 cases(68 males and 30 females)of Oriental Medical College at Kyunghee University. After determining the Sasang Constitutions(Soeumin, Soyangin, Taeumin) by QSCCII, the body shape of each group were analysed by body composition(INBODY 2.0, Biospace, seoul, korea) and body measurement. Results : 1) The result of Sasang Constitution analysis of 98 cases were classified as Soumin(52%), Soyang(24.5%), Taeumin(23.5%). 2) In the body composition and body measurement, there were significant difference from intercellular fluid, protein, mineral mass, weight, percent body fat, body water of left arm and BMI between Taeumin, Soyangin and Soumin. 3) In the body composition of male, Taeumin group showed that the arms were more developed than the legs and the upper trunk were more developed than the lower trunk. Soumin group showed the asymmetry of the right and left limbs. Soyang group showed the asymmetry of the right and left arms. In the body composition of female, Taeumin group showed the asymmetry of the right and left upper limbs. Conclusion : There are significant relationships between Sasang Constitutions, Body composition and BMI. The findings indicated here Body composition could be useful mean to make a diagnosis of the body shape, body type, physical constitution of Sasang constitutions.