가두리 양식장의 Vibrio vulnificus 검출 및 제어 방법

성치남,송계민,이규호,양성렬 한국미생물학회 2002 미생물학회지 Vol.38 No.4

2000년 1 월부터 2000년 10월까지 가두리 양식장에서 Vibrio vulnificus를 검출하였고 이들의 억제 방법을 연구하였다. 이 세균의 검출은 선택적 분리법과 vvhA 유전자를 확인하는 방법을 이용하였다. V. vulnificus는 수온이 $17^{\circ}C$이상인 5월부터 검출되었고 $19^{\circ}C$ 이상인 6월부터 9월까지는 대부분의 시료에서 검출되었다. V. vulnificus를 제어하기 위한 방법 중 냉동 및 냉장 처리는 살균효과를 나타내지 못했다. Citric acid도 균의 생장을 억제하지 못했으나, 500 mg/1이상의 EDTA가 첨가될 경우 균이 완전히 사멸되었다. 분말 광촉매인 산화티타니움은 자외선을 조사할 경우 15분~1 시간 이내에 이 세균을 완전히 사멸시키는 효과를 나타내었다. 산화티타니움을 유리 구슬에 코팅한 광촉매 장치를 이용하여 0.2/min의 turnover rate로 사멸효과를 얻었다. Detection of Vibrio vulnificus in fish farm and searching for the bactericidal methods on this bacteria were studied. To detect this microorganism in sea water, mud, fish and mussels, selective isolation methods and detection of vvhA gene were used from January to October,2000. V. vulnificus was detected from May when the water temperature was over $17^{\circ}C$. From June to September, higher than $19^{\circ}C$, this bacteria could be isolated from most of the samples. Freezing and refrigerating did not inhibit the growth of V. vulnificus. Citric acid did not show the bactericidal effect, but more than 500 mg/l of EDTA did. With the aid of UV and photocatalyst, $TiO_{2}$ showed bactericidal effect after 15 minute treatment. Photocatalytic system consisted of glass bead coated with $TiO_{2}$ and UV illumination showed bactericidal effect on V. vulnificus at the turnover rate of 0.2/min.

주암호의 수질 분석에 관한 연구

성치남,김종홍 순천대학교 기초과학연구소 1990 基礎科學硏究誌 Vol.1 No.-

This survey was conducted at 8 sites of the Juam reservoir through May, 1990 and October, 1990 to estimate the change of the water quality characteristics due to the construction of Juam dam. Physicochemical characters of river-ecosystem changed to those of lake-ecosystem. Water temperature, pH and B.O.D. of Juam reservoir increased as the result of the construction of Dam. Although the current trophic state of Juam reservoir is oligotrophic, that of influent streams is mesotrophic.

Taxonomic hierarchy of the phylum Proteobacteria and Korean indigenous novel Proteobacteria species

성치남,김미선,강주원,박희문 국립생물자원관 2019 Journal of species research Vol.8 No.2

The taxonomic hierarchy of the phylum Proteobacteria was assessed, after which the isolation and classification state of Proteobacteria species with valid names for Korean indigenous isolates were studied. The hierarchical taxonomic system of the phylum Proteobacteria began in 1809 when the genus Polyangium was first reported and has been generally adopted from 2001 based on the road map of Bergey’s Manual of Systematic Bacteriology. Until February 2018, the phylum Proteobacteria consisted of eight classes, 44 orders, 120 families, and more than 1,000 genera. Proteobacteria species isolated from various environments in Korea have been reported since 1999, and 644 species have been approved as of February 2018. In this study, all novel Proteobacteria species from Korean environments were affiliated with four classes, 25 orders, 65 families, and 261 genera. A total of 304 species belonged to the class Alphaproteobacteria, 257 species to the class Gammaproteobacteria, 82 species to the class Betaproteobacteria, and one species to the class Epsilonproteobacteria. The predominant orders were Rhodobacterales, Sphingomonadales, Burkholderiales, Lysobacterales and Alteromonadales. The most diverse and greatest number of novel Proteobacteria species were isolated from marine environments. Proteobacteria species were isolated from the whole territory of Korea, with especially large numbers from the regions of Chungnam/Daejeon, Gyeonggi/Seoul/Incheon, and Jeonnam/Gwangju. Most Halomonadaceae species isolated from Korean fermented foods and solar salterns were halophilic or halotolerant. Air-borne members of the genera Microvirga, Methylobacterium, and Massilia had common characteristics in terms of G+C content, major respiratory quinones, and major polar lipids.

남산 주요 식생의 토양 미생물의 분포 및 생리적 특성

성치남,백근식,김종홍,전영문,김정근 한국생태학회 1998 Journal of Ecology and Environment Vol.21 No.5

Physicochemical factors, microbial population size and the properties of the bacterial isolates were estimated to find out the nature of soil ecosystem of Mt. Nam. Samples were obtained from the surface layer of soils on which specific plant community is developed. Average content of moisture and organic matter of the soils were 21.6% and 17.3%, respectively. These values were similar to those of developing forest soils, but were slightly lower than those of climax ecosystem such as Piagol in Mt. Chiri. Chiri. Content of phosphate was higher than those of other forest soils. The population size of soil bacteria ranged from 27.4 to 195.8 ${\times}\;10^5$ CFU/g. duy soil, and the size is somewhat dependent on the moisture and oranic matter content of soils. A large number of bacteria were able to decompose macromolecules such as starch, elastin and gelatin. Bacterial species composition of each soil was comparatively simple. Pseudomonas, Agrobacterium, Flavobacterium and Xanthomonas which are Gram-negative short rods were widely distributed in the forest soils. The endospore forming Bacillus species were also the main constituents of the soil microflroa. Actinomycetes were widely distributed in the forest soils, but the distribution pattern varied in each site. Most of the actinomycetes were also able to decompose organic macromolecules. The rate of resistant actinomycete strains to antibiotics and heavy metals were lower than those from cultivated soils, but higher than those from well-preserved forest soils. Antibiosis pattern of the actinomycete isolates was similiar to the resistance pattern. This means the forest soils of Mt. nam was somewhat interferred by artificial behabiour.

백두산의 식생에 따른 토양 미생물의 분포 및 특성

성치남,백근식,김종홍 한국생태학회 1998 Journal of Ecology and Environment Vol.21 No.5

Physicochemical factors, microbial population size and the properties of the bacterial isolates were assessed to find out the nature of soil ecosystem of Mt. Paektu. Samples were obtained from the surface layer of soils on which specific plant community is developed. Average content of moisture, organic matter and avaiable phosphate of the soils were 21.6%, 17.3% and 2.48mg/100g, respectively. These values were similar to those of developing forest soils, but were slightly lower than those of climax ecosystem such as Piagol in Mt. Chiri. The population size of soil bacteria ranged from 2.7 to $202.5{\times}10^5$ CFU/g.dry soil, and the size is somewhat dependent on the content of moisture and oranic matter of the forest soil. A large number of bacteria was able to decompose macromolecules such as starch, elastin and gelatin. While the distribution rate of resistant bacteria to antibiotics was high, that to toxic chemicals was low. This means that the competition between microorgani는 predominate over the interference with artificial behaviour such as spread of pesticides in the surveyed region. Bacterial species composition of each soil was comparatively simple. Pseudomonas, Agrobacterium, Flavobacterium and Xanthomonas which are Gram-negative short rods were widely distributed in the forest soils. The endospore forming Bacillus species were also main constituents of the soil microflroa. any one of the strains was not identified as Azospirillum or Micrococcus which are known to be one of major constituents of the forest soil. for the correct identification of isolates chemotaxonomic studies will be proceeded, and the strains are to be stored in the Type collection Center.

[MINIREVIEW] Taxonomic hierarchy of the phylum Firmicutes and novel Firmicutes species originated from various environments in Korea

성치남,강주원,이지희,서소연,우정재,박철,배경숙,김미선 한국미생물학회 2018 The journal of microbiology Vol.56 No.1

This study assessed the taxonomic hierarchy of the phylum Firmicutes as well as elucidated the isolation and classification states of novel Firmicutes species isolated from Korean territory. The hierarchical classification system of the phylum Firmicutes has been developed since 1872 when the genus Bacillus was first reported and has been generally adopted since 2001. However, this taxonomic hierarchy is still being modified. Until Feb. 2017, the phylum Firmicutes consisted of seven classes (Bacilli, Clostridia, Erysipelotrichia, Limnochordia, Negativicutes, Thermolithobacteria, and Tissierellia), 13 orders, 45 families, and 421 genera. Firmicutes species isolated from various environments in Korea have been reported from 2000, and 187 species have been approved as of Feb. 2017. All Firmicutes species were affiliated with three classes (Bacilli, Clostridia, and Erysipelotrichia), four orders (Bacillales, Lactobacillales, Clostridiales, and Erysipelotrichales), 17 families, and 54 genera. A total of 173 species belong to the class Bacilli, of which 151 species were affiliated with the order Bacillales and the remaining 22 species with the order Lactobacillales. Twelve species belonging to the class Clostridia were affiliated within only one order, Clostridiales. The most abundant family was Bacillaceae (67 species), followed by the family Paenibacillaceae (56 species). Thirteen novel genera were created using isolates from the Korean environment. A number of Firmicutes species were isolated from natural environments in Korean territory. In addition, a considerable number of species were isolated from artificial resources such as fermented foods. Most Firmicutes species, belonging to the families Bacillaceae, Planococcaceae, and Staphylococcaceae, isolated from Korean fermented foods and solar salterns were halophilic or halotolerant. Firmicutes species were isolated from the whole territory of Korea, especially large numbers from Provinces Gyeonggi, Chungnam, and Daejeon.

주암호 지천의 수질과 어류군집

성치남,백근식,최지혁,조현욱,김종홍 ( Chi Nam Seong,Keum Sik Baik,Ji Hyeok Choi,Hyun Wook Cho,Jong Hong Kim ) 한국하천호수학회 1997 생태와 환경 Vol.30 No.2

Water quality and fish community in the streamlets of Juam reservoir were investigated in March, July, October and December 1997. Average value of dissolved oxygen, chemical oxygen demand and suspended solids was 10.1, 3.9 and 5.7 mg/l, respectively. Concentration of ammonium nitrngen varied with sites. Content of ammonium nitrogen, total nitrogen and phosphate-phosphorous of Site 4 where the waste water from Posung-up flows, was higher than that of any other sites. The number of collected fish species was 46 in 33 genera which belong to 12 families. Sixteen species in 13 genera were identified as Korean endemic species. Dominant species, Zacco platypus was collected in all sites. The largest number of species were collected in Site 10. Carassius auratus, Pseudorasbora parva and Z. platypus which have strong adaptablity to the environmental changes were collected in Site 4 where the species composition was simpler and the water was more polluted. Diversity and evenness indices ranged from 0.60 to 1.18 and from 0.28 to 0.75, respectively. These indices were low in the polluted sites. These results showed that water quality is related to some extent with species composition of fish.

A report of 38 unrecorded bacterial species in Korea, belonging to the phylum Actinobacteria

성치남,Mi-Sun Kim,Ji-Hee Lee,Joo-Won Kang,김승범,조장천,Jung-Hoon Yoon,Kiseong Joh,Chang-Jun Cha,임완택,Jin-Woo Bae 국립생물자원관 2016 Journal of species research Vol.5 No.2

As a subset work for the collection of indigenous prokaryotic species in Korea, 38 actinobacterial strains were isolated from various environmental samples obtained from plant root, ginseng cultivating soil, mud flat, freshwater and seawater. Each strain showed higher 16S rRNA gene sequence similarity (>99.1%) and formed a robust phylogenetic clade with closest actinobacterial species which were defined and validated with nomenclature, already. There is no official description on these 38 actinobacterial species in Korea. Consequently, unrecorded 37 species of 24 genera in the 12 families belonging to the order Actinomycetales of the phylum Actinobacteria were found in Korea. Morphological properties, basic biochemical characteristics, isolation source and strain IDs are described in the species descriptions.

대장균의 세포 밀도 의존 단백질의 검출

정재성,성치남 順天大學校 1989 論文集 Vol.8 No.1

대장균에서 세포 밀도에 따른 단백질 합성의 변화 양상을 SDS-Polyacrylamide Gel Electrophoresis를 이용하여 조사하였다. 그결과 세포의 밀도가 증가함에 따라 38 Kd의 단백질이 증가한 반면 36 Kd와 77Kd의 단백질의 양은 감소하였다. 단백질 합성을 억제시키는 항생제인 refampicine과 chloramphenicol을 처리한 후에도 38Kd의 단백질양은 감소하지 않았다. 따라서 38Kd의 단백질은 기존해 있던 단백질의 분해산물이 아니라 새로함성된 단백질임을 확인하였다. In E. coli, protein patterns changed according to population size at 23℃ and 37℃ were examined by SDS-PAGE. One 38Kd proteins band intensity was increased, while 36Kd and 77Kd proteins were diminished along with the increase of population size. When E. coli culture was treated prolonged time with antibiotics which block protein syntheses, there was no change on 38Kd protein band. These results suggest that 38Kd protein is not degradation product of preexistent protein but a newly synthesized protein.

계통학적 방벙을 이용한 우포습지의 원핵미생물 다양성

성치남(Chi Nam Seong),백근식(Keun Sik Baik),김미선(Mi Sun Kim),박성찬(Seong Chan Park) 한국토양비료학회 2006 한국토양비료학회 학술발표회 초록집 Vol.2006 No.5