http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
발생 중인 마우스 콩팥에서 Angiopoietin-1, Angiopoietin-2, 및 Tie2의 발현
김원 ( Kim Won ),이식 ( Lee Sig ),성미정 ( Seong Mi Jeong ),문상옥 ( Mun Sang Og ),김상균 ( Kim Sang Gyun ),장용범 ( Jang Yong Beom ),김성식 ( Kim Seong Sig ),강성귀 ( Kang Seong Gwi ),박성광 ( Park Seong Gwang ) 대한신장학회 2004 Kidney Research and Clinical Practice Vol.23 No.3
배경: Angiopoietins는 내피세포에 특이적으로 존재하는 수용체인 Tie2에 작용하는 ligands로서 발달과정에서 혈관형성에 관여한다. 혈관형성과정은 태생기에서 기관이 발달할 때 아주 필수적인 과정이지만 일부 후천적 질환에 관여하기도 한다. 방법: 신장은 혈관이 풍부한 장기이므로 혈관의 형성과정이 신장의 발달과정에 중요하기 때문에 이 연구에서는 신장의 발달 단계에 따라 angiopoietin-1 (Ang1), Ang2 그리고 Tie2 단백질을 시기적으로 위치적으로 발현 양상을 관찰하였다. 결과: Ang1 단백질은 태생 후 28일까지 지속적으로 증가하였고, Ang2 단백은 태생 후 14일째, Tie2 단백질은 태생 후 7일째 최고로 증가하였다. Ang1 단백질은 신수질과 피질의 세뇨관 상피세포 일부에서 발현하였고 Ang2 단백질은 피질의 세뇨관 상피세포와 사구체 일부에서 발현하였다. Tie2 단백질은 사구체내의 내피세포로 생각되는 부분과 수질부에서 vasa recta로 생각되는 부분에 발현하였다. 결론: 이상의 결과를 종합하면 Ang1, Ang2 그리고 Tie2은 발달과정의 신장에서 발현되어 있었다. Background : The Angiopoietin 1 (Angl). Angiopoietin-2 (Ang2) and Tie2 have essential role in angiogenesis in development. Ang1 and Ang2 are ligands which binds to their receptor. Tie2. Methods : Expression of these proteins was sought during mouse kidney maturation from embryonic day 16 (E16) to 28 days postnatal (P28). Results : Using RNase protection assay and Western blot, these three molecules were expressed throughout the experimental period with peak levels at P28 (Ang1), P14(Ang2) and P7 (Tie2). By immunohistochemical analysis, Ang1 protein was found to localize to condensing renal mesenchymal cells, and tubules, Ang2 proteins were detected in differentiating outer medullary tubules and the vasa recta bundle area. Tie2 protein was detected in a portion of glomerular tufts and cortical interstitium, and medulla including vessels in the vasa recta. Conclusions : These data suggest that Ang1, Ang2 and Tie2 proteins are expressed in renal development. (Korean J Nephrol 2004;23(3):385-395)
콜라겐 유도성 관절염 마우스모델에서 레스베라트롤 식이요법의 류마티스관절염 완화효과
천윤홍 ( Yun Hong Cheon ),김현옥 ( Hyun Ok Kim ),서영선 ( Young Sun Suh ),허재형 ( Jae Hyung Hur ),조원용 ( Won Yong Jo ),임혜송 ( Hye Song Lim ),하영술 ( Young Sool Hah ),성미정 ( Mi Jeong Sung ),권대용 ( Dae Young Kwon ),이상일 대한류마티스학회 2015 대한류마티스학회지 Vol.22 No.2
Objective. Resveratrol is well-known for its anti-inflammatory, anti-oxidant effects on several diseases. We investigated whether dietary supplementation with resveratrol may suppress joint inflammation and destruction in a mouse model of collagen-induced arthritis (CIA). Methods. Mice were randomly divided into two groups; CIA mice with normal diet-fed and CIA mice fed a 0.05% resveratrol diet. The effect of resveratrol on arthritis was assessed by clinical scoring system. The plain radiographs of paws were obtained to evaluate the effects on preventing bone destruction. Joint inflammation, cartilage damage, and osteoclastic bone resorption were checked by staining with H&E, Safranin-O, and tartrate resistant acid phosphatase (TRAP). Levels of pro-inflammatory cytokines were checked by enzyme-linked immunosorbent assay. The level of expression of nuclear factor (NF)-κB was measured by electrophoretic mobility shift assay (EMSA). Results. Dietary supplementation with resveratrol led to mitigated severity of arthritis compared to the normal diet group (6.7±0.8 vs. 2.7±0.6, p<0.01). Resveratrol-fed mice showed decreased bone destruction on radiograph (3.4±0.3 vs. 2.0±0.2, p<0.01), and showed significantly inhibited pathological changes (inflammation 2.0±0.3 vs.3.2±0.2, p<0.01; cartilage damage 1.5±0.3 vs. 3.2±0.2, p<0.01; pannus formation 1.4±0.3 vs. 3.0±0.3, p<0.01; erosion; 1.4±0.2 vs. 3.3±0.3, p<0.01). Generation of TRAP-positive osteoclasts was inhibited in the resveratrol-fed mice (55.3±12.7 vs. 3.27±0.8, p<0.01). Resveratrol-fed mice showed decreased levels of tumor necrosis factor-α, interleukin (IL)-1β, IL-6,monocyte chemoattractant protein 1, and the soluble receptor activator of NF- κB ligand in joint tissues and sera. Expression of NF-κB, measured by EMSA, was decreased in resveratrol-fed mice. Conclusion. Dietary supplementation with resveratrol mitigates inflammation and bone destruction in CIA mice. (J Rheum Dis 2015;22:93-101)