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      • KCI등재

        VEGF-overexpressed Human Tonsil-derived Mesenchymal Stem Cells with PEG/HA-based Cryogels for Therapeutic Angiogenesis

        선욱,최재훈,최영환,임성갑,소경하,Nathaniel S. Hwang 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.1

        Therapeutic neovascularization through cell transplantation has widely been investigated as a cure for ischemia. However, there are several limitations to this technique, including low survival rate of the therapeutic cells and inflammation at the transplanted sites. Polyethylene glycol (PEG)/hyaluronic acid (HA)-based cryogels are used to overcome these problems as they provide cell adhesion sites owing to their interconnected macroporous structure, which increases cell viability in combination with the cryogel’s intrinsic biocompatibility. Furthermore, vascular endothelial growth factor (VEGF) plays a significant role in pro-angiogenesis of endothelial cells (ECs) and it has been demonstrated that injection of VEGF-secreting cells could enhance neovascularization at the sites of injection. In the present study, we developed a PEG diacrylate (PEGDA)/glycidyl methacrylate-HA (GMHA)- based cryogel system seeded with VEGF-transfected human tonsil-derived mesenchymal stem cells to induce enhanced neovascularization in murine hind-limb ischemia. Addition of GMHA allowed the PEGDA cryogel to enhance cell metabolism, including attachment and proliferation. The secreted VEGF affected human umbilical vein endothelial cells by enhancing in vitro angiogenesis. Finally, our cellseeded cryogel successfully facilitated regeneration of blood flow and damaged tissue in a hind-limb ischemia model.

      • SCOPUSKCI등재

        안면횡단 신경이식술과 유리근육 피판술을 이용한 안면신경 마비 환자의 치험례

        김동일,최준,백세민,오갑성,정영덕,백롱민,선욱 大韓成形外科學會 1992 Archives of Plastic Surgery Vol.19 No.6

        The facial nerve paralysis means the loss of the important function dictated by this nerve, and so arises noticeable disturbances in functional, esthetic and social aspects. Especially the loss of facial muscular activities gives rise to the inability of emotional expression and the facial deformity as well as increasing the risk of exposure keratitis. It prevents patients with facial paralyses from making confident social interrelationships, and so leads them to the psychosocial shrinkage. The authors have operated on seven patients with several yerars old facial nerve paralyses by means of the cross-face nerve graft and the free vascularized muscle transfer. After one or two years of the follow up period, patients show almost symmetrical facial apperances at rest, improved ability of emotional expression and confident social interrelationships. We and patients have got the satisfactory result in functional, esthetic and social aspects.

      • SCOPUSKCI등재

        신연신생골 형성에 있어 신연속도에 따른 하악골의 영향

        하동호,정성훈,윤성호,김동일,선욱 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.1

        There have been great concerns about the application of distraction osteosynthesis to facial reconstruction, a technique which was proposed by Ilizarov in the early 20th century. Despite of many studies and trials since then, little research has been done concerning the specific response of facial bone to distracting stress. The purposes of this study are to investigate the maximal distraction rates and lengthening amounts which are safe from poor osteogenesis and possible adverse reaction. After corticotomy, eleven Korean mongrel dogs were distracted on their mandible for 28 days at different speeds: A group-3 dogs, 1.0 mm/4 times/day; B group-3 dogs, 1.5 mm/4 times/day; C group-2 dogs, 1.75mm /4 times/day; D group-3 dogs, 2.0 mm/4 times/day respectively, which were then examined in gross architecture, radiologic and histologic changes after 7 weeks fixation. The following results were obtained: dense osseous tissue was observed in new bone with the distraction rate of 2.0 mm/day. With maximum distraction rate and lengthening, most nerve fibers of the inferior alveolar nerve were well myelinated and had relatively normal axon structures in the region of the new bone except for rare findings of minimal disfiguration of axons and demyelination in some areas. There was not any evidence of arthrotic changes in temporomandibular joint under maximum distraction, but some adaptive changes were observed. In conclusion, relatively favorable bone formation was possible at the distraction rate of 2.0 mm per day. Because there was little damage of nerve and joint against maximum lengthening (43.4% of original bone length), this technique can be considered as a useful choice for reconstruction of larger defects on facial bone. However, a more systemized longitudinal analysis is needed to determine the regional effects of distraction and the detailed mechanism of ossification on the mandible.

      • P3HT, BDT-3T Polymer 고분자 유기박막태양전지에서의 안정도 및 효율감소에 대한 고찰

        선욱 ( Wook Sun ),이경균 ( Kyeong K. Lee ),이성구 ( Sung Koo Lee ),이효수 ( Hyo Soo Lee ),임은희 ( Eun Hee Lim ) 한국공업화학회 2010 응용화학 Vol.14 No.2

        In this writing, in order to understand stability/degradation in organic solar cell devices, the device stability of poly(3-hexylthiophene) P3HT and poly(benzo[1,2-b:4,5-b` ]dithiophene- alt-terthiophene (BDT-3T) is discussed. The OPVs were fabricated using the polymers and [6,6]-phenyl-C61-butyricacidmethylester (PC[60]BM) as donor and acceptor, respectively. The effect of O(2) on device stability was examined by keeping the P3HT and BDT-3Tdevices in N(2) and airand the humidity was controlled by introducing getter on encapsulation of device. The experiment was done until thousands of hours. The device stabilityof new copolymer BDT-3T was comparable to that of P3HT.

      • KCI등재후보

        미용 수술 후 발견된 쿠싱 증후군 환자의 비정상적 창상 치험례

        최민,선욱,김진우,정재학,김영환 대한미용성형외과학회 2009 Archives of Aesthetic Plastic Surgery Vol.15 No.2

        Cushing syndrome has several adverse effect such as infection and delayed wound healing. However, it can't be easily detected by routine screening test. Our department reports a case of abnormal wound healing of Cushing syndrome that was found after face lift and abdominoplasty. A 55-year-old woman presented with aging face and abdominal obesity. Pre-operative screening test was normal except thin and dry skin. MACS(minimal access cranial suspension) lift and high tension abdominoplasty were done. After surgery, preauricular and abdominal flaps were deepithelialized and necrotized despite of stitch out and conservative dressing. To find the reason of abnormal wound healing, we conducted additional studies including hormone and CT. A 30mm sized adrenal cortical tumor was found on CT scan, and she was diagnosed as Cushing syndrome. After removal of the tumor, wound was stabilized. Therefore, full thickness skin graft and local flap were done. Wound healing became normal, and major complications such as dehiscence and infection did not occur. Abnormal wound healing process caused by Cushing syndrome can be normalized after resection of adrenal tumor.

      • KCI등재

        성형수술과 관련된 의료분쟁 사례 분석

        최민,선욱 대한성형외과학회 2009 Archives of Plastic Surgery Vol.36 No.3

        Purpose: Recently medical dispute about plastic surgery is increasing rapidly as result of growing surgery itself due to high interest in appearance and advertisement of plastic surgery. So we want to find a way to prevent similar medical accident by making better solution of plastic surgery through case analysis of medical dispute. Method: 161 cases about plastic surgery asked for aid at Korea Consumer Agency and 41 cases judged at the court was surveyed and gender, location and kind of hospital, goal and kind of operation, making operation agreement or not ,kind of damage, result of process, result of lawsuit was studied from 2004.1.1. to 2006.12.31. Result: Medical doctor have to explain about symptoms of disease, method of treatment, possibility of complication, prognosis to patients before therapy so they can make decision if they take operation by doctor or not. On this survey, among the patients who underwent re - operation or had complication, 88.1%(96 from 109 cases) of them didn't get enough explanation about possibility of complication before surgery They brought lawsuit insisting they would not undergo operation if they got enough explanation about possibility of complication and result of operation before surgery. Conclusion: It is advisable that doctor must observe the duty of explanation before surgery and respect the right to decide of patient, make operation agreement and put down concrete progress note and store the pre and post operative photo to avoid medical accident. It is also needed to have guideline of therapy, Code of ethics, organization which deals with medical dispute, reconsideration of law to control that.

      • SCOPUSKCI등재

        광범위 이중층 안면 거상술(extended Radical Double layer face Lift)의 임상적 고찰

        백세민,선욱,최준,김순걸,박성규,오갑성 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.6

        Rhytidectomy has been researched as the one of the methods to overcome aging process. In spite of the various trials of rhytidectomy beyond time, the basic principle is dissection and lift. The major different point among them is the dissection palne, which can be classified as subcutaneous, sub-SMAS and its continuous layer, and subperiosteal plane. We have experienced 283 cases of rhytidectomy through various dissection plane from March 1988 to Feburary 1993. Among them, 93 cases were performed through double-layer lift technique, the most characteristic feature of which is double layer dissection in temporal and midface area. Double layer dissection was performed under and above superficial temporal fascia in temporal area, under and above SMAS in masseteric area, and subperiosteal and subcutaneous in the other midface area. According to our experience of double layer lift, we could obtain the advantages of subperiosteal lift such as long lasting effect, natural appearance and accessibility to contour facial bone by lift of periosteum and other structure to support skin. Also, we achieved the divect rhytidectomy effect by subcutaneous lift and we could control the direction of lift with combination of subperiosteal lift.

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