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김민근,석수원,이영한,김혜란,조계만 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.1
In 2009-2010, unusual symptoms were observed onPleurotus eryngii grown in mushroom farms in GyeongnamProvince, Republic of Korea. One of the main symptomswas a cobweb-like growth of fungal mycelia over thesurface of the mushroom. The colonies on the surfacerapidly overwhelmed the mushrooms and developedseveral spores within 3-4 days. The colonized surfaceturned pale brown or yellow. The fruit body eventuallyturned dark brown and became rancid. Koch’spostulates were completed by spraying and spottingusing isolated strains. The phylogenetic tree obtainedfrom the internal transcribed spacer sequence analysisshowed that the isolated fungal pathogen correspondedto Cladobotryum mycophilum (99.5%). In the fungicidesensitivity tests, the ED50 values for the isolate withrespect to benomyl and carbendazim were from 0.29 to0.31 ppm. Benzimidazole fungicides were most effectiveagainst C. mycophilum, a causal agent of cobweb diseasein P. eryngii.
큰느타리버섯 단핵균주 간 교잡에 의한 저장우수형 품종 “단비5호” 육성 및 특성
이영한,김야엘,석수원,정정민,류재산,허재영,김희대,최용조,김민근 한국버섯학회 2016 한국버섯학회지 Vol.14 No.2
큰느타리버섯 품종 육성을 위해 품질이 우수한 특성을 지니는 큰느타리버섯 KNR2598과 경도가 우수한 특성을 지니는 큰느타리버섯 KNR2610 모본으로부터 단핵균주를 분리 한 뒤 단포자간 교잡을 통해 고품질의 저온 저장성이 우수한 신품종 “단비5호”를 육성하였다. 신품종의 균사 생 육 적정온도는 25 o C이며 자실체 발생 적정 온도는 15~ 16 o C 이었다. 솎음 재배에서 발이소요일수를 포함한 수확 소요일수는 대조품종인 큰느타리버섯 2호에 비해 0.5~1.7 일 정도 빠른 특성을 보였다. 갓 색깔은 중간수준의 갈색이 며 대 색깔은 흰색을 나타내었다. 갓모양은 우산형으로 850cc 플라스틱 병재배에서 한 개체의 평균무게는 90.4 ±16.9 g이었다. “단비5호”와 대조품종간의 RAPD 분석결과 서로 다른 DNA 밴드양상을 보여 주었으며 대치배양을 통 해 두품종간에 대치선을 확인 하였다. “단비5호”의 경우 4 o C에서의 저장성이 대조품종 대비 24.6~30.6일 이상 길게 유지되는 특성이 있어 수출적합형 품종으로 가치가 있을 것으로 기대된다. Pleurotus eryngii is one of the most widely cultivated and important edible mushrooms. However, the shelf life of the fruiting body is short, which . To solve this problem, a new cultivar, DanBi 5Ho, of Pleurotus eryngii was developed by monomono crossing between monokaryotic strains derived from KNR2598 and KNR2610. The optimum temperature formycelial growth was 25 and that forfruiting body development was 15–16. The quality did not change after a period of 40.0 d at 4. This result was significantly different compared to that ofthe control strain Knneuari 2 Ho. Analysis of genetic characteristics of the new hybrid strain DanBi 5Ho revealeda different profile thanthat of the parental and control strains when random amplification of polymorphic DNA (RAPD) primers we used. The results obtained from this study show that DanBi 5Ho is a new hybrid strain, characterized by improved storability after harvesting.
김민근,이선미,석수원,류재산,김희대,권진혁,최용조,윤한대 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.3
RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the Enterobacteriaceae. The rcsA gene of the soft rot pathogen Pantoea sp. strain PPE7 in Pleurotus eryngii was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins. The inactivation of rcsA by insertional mutagenesis created mutants that had decreased production of EPS compared to the wildtype strain and abolished the virulence of Pantoea sp. strain PPE7 in P. eryngii. The Pantoea sp. strain PPE7 rcsA gene was shown to strongly affect the formation of the disease symptoms of a mushroom pathogen and to act as the virulence factor to cause soft rot disease in P. eryngii.
Effect of Bacillus subtilis S37-2 on Microorganisms in Soil and Growth of Lettuce (Lactuca sativa)
허재영,김대호,최용주,이상대,석수원,송재경,권장식,김민근 한국토양비료학회 2016 한국토양비료학회지 Vol.50 No.4
The present study evaluated the variations in soil microbial population of controlled horticultural land used for lettuce (Lactuca sativa) cultivation by their fatty acid methyl ester and chemical properties. We utilized four treatment groups, no treatment (NT), culture medium (CM), Bacillus subtilis S37-2 (KACC 91281P) × 106 CFU mL-1 (BS1), and Bacillus subtilis S37-2 × 107 CFU mL-1 (BS2) and analyzed these variations throughout the before treatment and harvesting stage. The chemical properties such as pH, organic matter, available phosphate, and electrical conductivity in soils before treatment and harvesting stage showed no significant difference among the treatments. Total numbers of bacteria and microbial biomass C in soil treated with BS1 were larger than those of NT, CM, and BS2, whereas total number of fungi at the harvesting stage was significantly lower in the BS1 soil than in the NT and CM soils (P < 0.05). On basis of leaf length, leaf width, leaf number and leaf weight, the growth characteristics lettuce on the soil treated with BS1 and BS2 was faster than those of NT and CM soils. Yield of lettuce with treated BS1 and BS2 were 35% and 29% more than that of NT, respectively.