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서해안 천수만 일대 양식 조피볼락(Sebastes schlegeli)의상피 선충 감염현황
서한길 ( Han Gill Seo ),서정수 ( Jung Soo Seo ),류민경 ( Min Kyung Ryu ),이은혜 ( Eun Hye Lee ),권세련 ( Se Ryun Kwon ),강종순 ( Jong Soon Kang ),노윤산 ( Yun San No ),최혜승 ( Hye Sung Choi ),정승희 ( Seung Hee Jung ),한현자 ( Hy 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5
We investigated a nematode infection in the epithelial tissue of rockfish Sebastes schlegeli cultured in Cheonsu Bay, western Korea, from May 2013 to April 2014. Nematodes infected the epithelial tissues of various external organs, including the fins, operculum, nares, mouth, and head. Over a 1-year period, the overall nematode infection rate in rockfish was 55% (n=89/163): 56.7% (n=17/30) in May 2013, 88% (n=29/33) in August 2013, 0% (n=0/30) in October 2013, 70% (n=21/30) in January 2014, and 55% (n=22/40) in April 2014. During this study, the only massmortality of cultured rockfish in Cheonsu Bay was in August 2013, when we identified skin wounds on the rockfish caused by the nematodes escaping from their hosts. During this period, the accumulated mortality for 2 weeks was1.4-22.4% in different farms. In addition, several pathogenic bacteria (Photo bacterium damselae, Vibrio spp., and Streptococcus iniae) were isolated from the moribund rockfish; these were thought to be transmitted through the skin wounds caused by the nematodes.
양식 가숭어(Chelon haematocheilus)에서 최초로 분리된 갈고리벌레과 Cymothoids의 특성 연구
서한길 ( Han-gill Seo ),오명주 ( Myung-joo Oh ),조미영 ( Miyoung Cho ),한현자 ( Hyun-ja Han ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2
Mullet is an important marine aquaculture fish species in Korea, with a total of 7,237 tons produced as of 2022, making it the 5th most produced marine aquaculture fish species. In this study, ectoparasites presumed to be isopods were discovered in the fins of farmed flathead grey mullet (average weight 550 g), and the characteristics of the parasites were confirmed. The length of the parasite was 5 to 18 mm, and 3 to 7 parasites were infected per fish. To analyze the characteristics of the parasites, molecular biological identification and phylogenetic analysis were performed using the cytochrome c oxidase subunit I (COI) gene, and it was confirmed to be most closely related to Nerocila japonica in the Cymothoidae family. To confirm the parasite control effect, a direct exposure drug sensitivity test was conducted on five types of aquatic drugs and fresh water, trichlorfon was confirmed to be effective.
참조기(Larimichthys polyactis)에서 분리된 Neobenedenia spp.에 대한 분자 생물 동정 및 계통수 분석
서한길,김효원,김정현,Seo, Han-Gill,Kim, Hyo-Won,Kim, Jung-Hyun 한국어병학회 2022 한국어병학회지 Vol.35 No.1
In this study, we determined the cause of a disease outbreak in small yellow croaker(Larimichthys polyactis) in Jeju island. The major external signs in the dead fish were hemorrhage of the skin. Vibrio harveyi were isolated from a few fishes and viruses were not detected from the diseased fish. However, flukes were confirmed on the skin and we conducted molecular identification and phylogenetic analysis of the isolated parasites. The obtained 28S rRNA sequence of our specimen(Accession No. OM333244) showed the highest homology with Neobenedenia girellae, while the COI sequence of our specimen showed the highest homology with N. melleni. Further sequence analysis with other genes and morphological observation are necessary for accurate identification.
신경괴사증바이러스(Nervous Necrosis Virus, NNV) 모니터링을 통한 무감염 능성어(Hyporthodus septemfasciatus)친어의 선발
김시우,김위식,서한길,김경민,오명주,Kim, Si-Woo,Kim, Wi-Sik,Seo, Han-Gill,Kim, Kyong Min,Oh, Myung-Joo 한국수산과학회 2017 한국수산과학회지 Vol.50 No.5
We investigated the infection of nervous necrosis virus (NNV) in seven band grouper Hyporthodus septemfasciatus broodstocks, which have been reared in aquaculture farms in South Korea during 2012-2014. To investigate the prevalence of NNV within the broodstock, egg, sperm, and blood were sampled in the spawning season. The egg and sperm samples were subjected to a nested reverse transcription (RT) polymerase chain reaction (PCR) assay to detect NNV and were inoculated on SSN-1 cells to culture the virus. Blood samples were used to detect antibodies against NNV using enzyme linked immunosorbent assay (ELSIA). Positive values from ELISA were found in 39 of 162 samples (24%) in 2012, and 13 of 28 samples (46%) in 2014. Additionally, 4 of 34 broodstocks (11%) investigated in 2013-2014 were determined to be carriers from the nested RT-PCR and in vitro cultivation. The broodstocks in which antibodies against NNV were detected by ELISA, or in which NNV was detected by the nested RT-PCR assay, posed a risk of vertical transmission of NNV. Therefore, it is necessary to select virus-free broodstocks in seed production to reduce the possibility of the vertical transmission of NNV.