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실험연구 : 백서 피질 신경원 혼합배양 모델에서 α-amino-5-methyl-4-isoxazolepropionate로 유도된 뇌독성에 대한 Propofol의 효과
서명신 ( Myoung Sin Seo ),박성용 ( Sung Yong Park ),김계숙 ( Kye Sook Kim ),문봉기 ( Bong Ki Moon ),김진수 ( Jin Soo Kim ),이숙영 ( Sook Young Lee ) 대한마취과학회 2008 Korean Journal of Anesthesiology Vol.55 No.5
Background: The pattern of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated neurotoxicity (necrosis vs apoptosis) and the neuroprotective effect of propofol on AMPA-mediated neurotoxicity are still unclear. Methods: Thirteen-day-old primary rat mixed cortical cultures were used. To measure the neuroprotective effect of propofol, AMPA (50 μM), AMPA (50 μM) plus propofol (0.1, 1, 25, 50 μM), AMPA (50 μM) plus DMSO, propofol (50 μM) and DMSO were administered (n=45). Seventy-two h later, surviving cells were counted using trypan blue staining and were converted to cell death rate (CDR). To measure the effect of propofol (50 μM) on AMPA (50 μM)-induced apoptosis, a triple stain was done. In a fixed field (×400), the number of neuronal cells stained by neuronal nuclei (NeuN) and Hoechst staining and apoptotic cells stained by terminal deoxynucleotidyl transferase mediated dUTP nick-end-labeling (TUNEL) assays were counted. Apoptotic cell rates (ACR) were also calculated. Statistical analyses were performed using one way-analysis of variance followed by Bonferroni`s test. P<0.05 was considered statistically significant. Results: AMPA (50 μM) stimulation demonstrated 49.3% CDR, and adding propofol 50 μM decreased CDR to 29.4% (P<0.05). In the TUNEL assay, cells with no drug treatment demonstrated 12.3% ACR and 50 μM AMPA increased ACR to28% (P<0.05). Adding 50 μM propofol to AMPA decreased the ACR to 20.1% (P<0.05). Conclusions: Propofol (50 μM) had neuroprotective effects against AMPA (50 μM)-induced cell death by reducing apoptosis. (Korean J Anesthesiol 2008;55:607~12)