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서동혜,Kim Sojung,Seo Hyeon Ji,Lee Seung Eun,임철진,Yang Hee Woong,CUILIHUA,안민용,Yu Seong Gwan,Cho Na Hyun,Park So Young,Lee Ji Hee,Kim Woo Taek 한국식물학회 2022 Journal of Plant Biology Vol.65 No.1
The liverwort Marchantia polymorpha, as the earliest land plant, is an important model system for studies of molecular evolution. Liverworts, which undergo a haplo-diplontic life cycle, spend most of their life as haploid gametophyte bodies, called thalli. In addition, the thalli can produce vegetative propagules, termed gemmae, conferring asexual reproduction in liverworts. Thus, to obtain sufficient isogenic tissues for genetic manipulation, the thallus is a suitable material. Here, we found that 23- to 25-d-old fully expanded thalli can be used for the genetic transformation with the pre-cultivation process, setting up the mature thalli pre-cultivation method. Next, we elucidated that 10- to 12-d-old young thalli can be used for the tissue-culture-based transformation in the absence of the pre-cultivation step, establishing the young thalli direct co-culture method without significant changes of transformation efficiency compared with the mature thalli pre-culture method. Overall, we set up the protocol using various stages of thalli of M. polymorpha with stable transformation efficiency. Mainly, we focused on reducing the hands-on labor time for performing the genetic transformation by widening the available stage of thalli for transformation or excluding the regenerating process of the thallus plantlets. In this report, we describe the detailed procedures employed under experimental conditions.
연중특별기획: 몸을 망가뜨리는 인간의 치명적 실수 무관심 - 휴가지에서 혹사당하는 피부, 올 여름엔 제대로 챙겨보자!
서동혜,Seo, Dong-Hye 한국건강관리협회 2010 건강소식 Vol.34 No.7
뜨거운 태양과 새하얀 해변, 부서지는 파도. 상상만으로도 가슴 설레는 여름휴가가 눈앞에 다가왔다. 시원하고 달콤 알싸한 청량음료의 짜릿함처럼 신나는 휴가를 만끽해보자. 하지만 휴가지에서 혹사당하는 피부에도 신경을 쓰자. 일광화상으로 인한 피부 트러블을 휴가의 증표로 남기지 않으려면 이번 여름휴가에서는 트러블과 화상이 생기지 않도록 유의하자.