원발성 신경교육종에 대한 단일기관의 임상성적

변준호 ( Joon Ho Byun ),윤정호 ( Jung Ho Yun ),조영현 ( Young Hyun Cho ),김정훈 ( Jeong Hoon Kim ),김창진 ( Chang Jin Kim ) 대한뇌종양학회 대한신경종양학회 2014 Brain Tumor Research and Treatment Vol.2 No.1

Objectives:Primary gliosarcoma (GSM) is a rare central nervous system tumor with few reports described in the literature. Here, we present our clinical experience and the outcome of primary GSM patients in a single medical center. Method:Primary GSM patients who were treated at our institution between 1990 and 2011 were selected retrospectively. All patients were analyzed by meticulous chart review including clinical and pathologic data. Overall survival was analyzed by Kaplan-Meier survival analysis. Results:Eleven patients were diagnosed with primary GSM. It was 1.7% of glioblastoma (GBM) during the same period. The overall median survival of the 11 patients was 12 months (range : 3 to 177 months). Patients who underwent maximal safe resection had a prolonged median survival compared to those who only underwent biopsy of the tumor (12 vs. 6 months, p=0.288). All patients received adjuvant postoperative radiotherapy and 7 of 11 patients received chemotherapy with temozolomide. Four patients received gamma-knife radiosurgery for local recurrence; however, treatment method by gamma-knife radiosurgery was not significantly associated with prolonged survival in this study. Patients who received re-explorative surgery for resectable recurred tumors had a prolonged median survival compared to those who had non-resectable recurred tumors (14 vs. 6 months, p=0.0082). Conclusion:We present 11 primary GSM patients who were treated according to the standard treatment for GBM. Despite aggressive multimodality treatment, the life expectancy of GSM is very short until now. Therefore, large and comprehensive clinical investigations are needed to establish the standard treatment for primary GSM.

임상 : 원발성 신경교육종에 대한 단일기관의 임상성적

변준호 ( Joon Ho Byun ),윤정호 ( Jung Ho Yun ),조영현 ( Young Hyun Cho ),김정훈 ( Jeong Hoon Kim ),김창진 ( Chang Jin Kim ) 대한뇌종양학회 2012 대한뇌종양학회지 Vol.11 No.2

Objectives:Primary gliosarcoma (GSM) is a rare central nervous system tumor with few reports described in the literature. Here, we present our clinical experience and the outcome of primary GSM patients in a single medical center. Method:Primary GSM patients who were treated at our institution between 1990 and 2011 were selected retrospectively. All patients were analyzed by meticulous chart review including clinical and pathologic data. Overall survival was analyzed by Kaplan-Meier survival analysis. Results:Eleven patients were diagnosed with primary GSM. It was 1.7% of glioblastoma (GBM) during the same period. The overall median survival of the 11 patients was 12 months (range : 3 to 177 months). Patients who underwent maximal safe resection had a prolonged median survival compared to those who only underwent biopsy of the tumor (12 vs. 6 months, p=0.288). All patients received adjuvant postoperative radiotherapy and 7 of 11 patients received chemotherapy with temozolomide. Four patients received gamma-knife radiosurgery for local recurrence; however, treatment method by gamma-knife radiosurgery was not significantly associated with prolonged survival in this study. Patients who received re-explorative surgery for resectable recurred tumors had a prolonged median survival compared to those who had non-resectable recurred tumors (14 vs. 6 months, p=0.0082). Conclusion:We present 11 primary GSM patients who were treated according to the standard treatment for GBM. Despite aggressive multimodality treatment, the life expectancy of GSM is very short until now. Therefore, large and comprehensive clinical investigations are needed to establish the standard treatment for primary GSM.

원발성 두개강 내 간엽성 연골육종 증례 보고

변준호 ( Joon Ho Byun ),조영현 ( Young Hyun Cho ),김정훈 ( Jeong Hoon Kim ),김창진 ( Chang Jin Kim ) 대한뇌종양학회·대한신경종양학회·대한소아뇌종양학회 2011 대한뇌종양학회지 Vol.10 No.2

Primary intracranial mesenchymal chondrosarcoma (MCS) is very rare neoplasm and it is difficult to differentiate from meningioma or hemangiopericytoma due to its rarity and indistinctive radiologic features. A 29-year-old woman was ad-mitted to the hospital with 3 month history of headache. CT scans revealed a 5.5×4.0 cm-sized hyperdense mass with in-tratumoral calcification at right frontal lobe. On MRI, the tumor represented iso to low signal intensities on both T1-and T2-weighted images and intense enhancement. Complete extirpation was done. The histopathologic examination estab-lished the diagnosis of MCS. Typical primary intracranial MCSs occur in supratentorial area, most often in the frontopa-rietal region. Usually, MCSs are hypervascular, attached to the meninges, and occasionally accompanied osteolytic bony defect. In our case, tumor was not attached to meninges, and there was no osteolytic lesion. The preferred treatment is radical resection. The benefit of radiotherapy and chemotherapy remains controversial.

미세주입을 이용한 난자로의 분리된 미토콘드리아 전달

백상기(Sang-Ki Baek),변준호(June-Ho Byun),김보규(Bo Gyu Kim),이아람(A ram Lee),조영수(Young-Soo Cho),김익성(Ik-Sung Kim),서강미(Gang-Mi Seo),정세교(Se-Kyo Chung),이준희(Joon-Hee Lee),우동균(Dong Kyun Woo) 한국생명과학회 2017 생명과학회지 Vol.27 No.12

고구마 현탁배양세포의 EST library에 높은 빈도로 존재하는 metallothionein (MT) 유전자를 선별하였다(IbMT3). MT 유전자는 세포와 조직의 스트레스 조절과 연관되어 있다고 알려져 있다. 본 연구에서 IbMT3 cDNA의 전장을 확보하여 염기서열 분석을 한 결과, IbMT3 유전자는 구조적으로 유형 3에 속하는 MT 단백질을 암호화하고 있었다. 식물에서 유형 3에 속하는 MT 단백질의 기능은 명확히 알려지지 않다. Northern blot 분석 결과, IbMT3 유전자는 고구마 식물체 잎보다 현탁배양 세포에서 매우 강하게 발현되었다. 일반적으로 세포배양은 세포에 산화 스트레스 상태를 부과하는 것으로 알려져 있다. 이에, 고구마 식물체에 산화 스트레스를 처리하여 IbMT3 유전자의 발현이 어떻게 조절되는지 조사하였다. 제초제인 methyl viologen (MV)을 6, 12, 24시간 동안 처리했을 때, IbMT3 유전자의 발현은 6시간 후에 아주 강하게 유도되었고 그 이후에는 감소함을 알 수 있었다. 저온 스트레스(15℃)를 24, 48시간 동안 처리했을 때, IbMT3 유전자는 처리시간이 경과함에 따라 발현이 더 많이 유도되었다. 이로써, IbMT3 유전자는 환경 및 산화 스트레스에 반응하여 발현이 조절되는 유전자임을 알 수 있었다. IbMT3 isoform은 고구마 식물체 내에서 항산화제로써 작용할 가능성이 있을 뿐 아니라, 스트레스 하에서의 세포 적응 메커니즘에 중요한 기능을 할 것으로 사료된다. Mitochondria play a central role in energy generation by using electron transport coupled with oxidative phosphorylation. They also participate in other important cellular functions including metabolism, apoptosis, signaling, and reactive oxygen species production. Therefore, mitochondrial dysfunction is known to contribute to a variety of human diseases. Furthermore, there are various inherited diseases of energy metabolism due to mitochondrial DNA (mtDNA) mutations. Unfortunately, therapeutic options for these inherited mtDNA diseases are extremely limited. In this regard, mitochondrial replacement techniques are taking on increased importance in developing a clinical approach to inherited mtDNA diseases. In this study, green fluorescence protein (GFP)-tagged mitochondria were isolated by differential centrifugation from a mammalian cell line. Using microinjection technique, the isolated GFP-tagged mitochondria were then transferred to bovine oocytes that were triggered for early development. During the early developmental period from bovine oocytes to blastocysts, the transferred mitochondria were observed using fluorescent microscopy. The microinjected mitochondria were dispersed rapidly into the cytoplasm of oocytes and were passed down to subsequent cells of 2-cell, 4-cell, 8-cell, morula, and blastocyst stages. Together, these results demonstrate a successful in vitro transfer of isolated mitochondria to oocytes and provide a model for mitochondrial replacement implicated in inherited mtDNA diseases and animal cloning.

인공신경망과 CFD 시뮬레이션을 이용한 Cargo Oil Tank Vent Pipe 용 Anti-splash Device 성능 개선

김민우(Min Woo Kim),나옥균(Ok Kyun Na),변준호(Jun Ho Byun),박종환(Jong Hwan Park),박준홍(Joon Hong Park),박영철(Young Chul Park) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.4-2

시각단서에 따른 다운증후군 아동의 보행패턴 변화

변준호,김기홍 용인대학교 2011 특수체육연구 Vol.9 No.-

The purpose of this study is to analyze and compare the walking patterns of the child with Down syndrome and the children without disabilities and to examine the effects of the visual cues on changes of walking pattern of the child with Down syndrome. The subjects were one child with Down syndrome of Trisomy and five children without disabilities. Before the experiment, seven real-time infrared rays cameras (Vicon I.R., Strobe & Pus, MX13) were established in front, rear, left, right, and diagonal directions. During walking by children, five movements appropriate were chosen for the comparative analysis. To calculate the average and standard deviation of all data, Vicon System’s Polygon was used. The results from this procedure are as follows: First, walking speed per minute of children with Down syndrome was slower than that of the children without disabilities. Second, stride length of child with Down syndrome and stride length of the children without disabilities increased in normal walking and in red lighted condition and decreased in line walking condition. Third, the change of walking speed of the child with Down syndrome showed bigger differences than that of the children without disabilities. Fourth, flexion-extension angle of hip joint of the child with Down syndrome showed similar changes comapring to those of the children without disabilities over the progress of normal, red, and line walking. Fifth, the average of changes in flexion-extension angle of hip joint of the child with Down syndrome showed decrease in angles than those of the children without disabilities in all events over the progress of normal, red, and line walking conditions. Sixth, the average of angle changes in ankle joint of the child with Down syndrome showed decreasing differences comparing that of the children without disabilities over the progress of normal, red, and line walking. In the case of line walking, the closest result to range of motion exercise of the children without disabilities was shown. 본 연구는 시각단서에 따라 다운증후군 아동과 일반아동의 보행패턴을 비교 분석하여 시각단서가 다운증후군 아동 보행 패턴의 변화에 미치는 영향을 알아보는 것이다. 이를 위해 삼염색체 다운증후군을 가지고 있는 아동 1명과 일반 아동 5명을 대상자로 선정하였다. 본 실험에 들어가기 전 운동학적 변인분석을 위하여 전, 후, 좌, 우, 대각선 방향으로 리얼타임 적외선 카메라(Vicon I.R., Strobe & Pus, MX13) 7대를 설치하였다. 실험 실시 전, 연구대상들에게 동작수행에 적합한 타이즈로 환복 하도록 하였으며 익숙하지 않은 환경에 적응하기 위한 적응기를 거친 뒤 전신에 39개의 반사마커를 부착한 후, 전·후 6m의 보행주로를 시각과제 유형에 따라 각각 10회 씩 걷도록 하였다. 시각단서에 따른 다운 증후군 아동과 일반아동들의 보행 중 실험 목적에 적합한 총 5회의 동작을 선별하여 보행 패턴을 비교분석하였으며 이 때, 모든 데이터의 산출 및 평균과 표준편차는 Vicon System의 Polygon을 사용하였다. 이와 같은 절차를 통하여 얻어진 결론은 다음과 같다. 첫째, 다운증후군 아동의 분당 걸음속도는 일반아동의 분당 걸음속도에 비해 느리다. 둘째, 다운증후군 아동의 활보장과 일반아동의 활보장 모두 Normal 보행과 Red 보행 시 커졌다가 Line 보행 시에는 다시 줄어든다. 셋째, 다운증후군 아동의 보행 속도 변화는 일반 아동의 보행속도 변화 보다 차이가 크게 나타난다. 넷째, 다운증후군 아동에 엉덩관절의 굴곡·신전각도는 Normal, Red, Line 으로 진행되어감에 따라 일반 아동집단에 엉덩관절의 굴곡·신전각도 변화와 유사하게 나타난다. 다섯째, 다운증후군 아동의 무릎관절에 굴곡·신전각도 변화의 평균은 Normal, Red, Line 으로 진행되어감에 따라 모든 이벤트에서 일반아동집단과의 각도 차이가 줄어든다. 여섯째, 다운증후군 아동의 발목관절에 각도 변화의 평균은 Normal, Red, Line 으로 진행되어감에 따라 모든 이벤트에서 일반아동집단과의 각도 차이가 줄어들며, Line 보행 시 일반아동집단의 관절가동범위에 가장 가까운 결과가 나타난다.