한국 재래 산양에 있어서 Prostaglandin $F_{2\alpha}(PGF_{2\alpha})$의 투여가 호르몬 함량 및 난포의 발육에 미치는 영향

변명대,함태수 한국동물번식학회 2001 Reproductive & developmental biology Vol.25 No.2

These experiments were conducted to evaluate the effects of the administration of exogenous PG $F_{2{\alpha}}$ on hormone concentrations and follicular development in early postpartum(pp) Korean native goats. 1. Plasma PG $F_{2{\alpha}}$ concentrations in PG $F_{2{\alpha}}$ treated goals showed a gradual increase from a low on day 2 (GR-1 : 10 day-treatment group: 6.35$\pm$0.5 and GR-2; 4 day-treatment group: 0.22$\pm$0.2 pg/$m\ell$, respectively) to reach a peak of 21.18$\pm$1.6 or 4.21 $\pm$0.4 pg/$m\ell$ on day 4 after treatment of PG $F_{2{\alpha}}$. 2. Plasma PG $F_{2{\alpha}}$ concentrations in GR-1 goats averaged 9.08 $\pm$1.2 pg/$m\ell$ compared with 5.44$\pm$ 1.8 pg/$m\ell$ in GR-2 goats the day before treatment of PG $F_{2{\alpha}}$. Mean PG $F_{2{\alpha}}$ concentrations thereafter remained low during the treatment period but PG $F_{2{\alpha}}$ concentrations did not differ between the two group. 3. Plasma concentrations of estradiol-17 $\beta$ (E,) in PG $F_{2{\alpha}}$ - treated group were decreased gradually until 2 days after PG $F_{2{\alpha}}$ treatment but mean $E_2$ concentrations began to increase on 3 days and were Inaximal on the 4 days after treatment. 4. Plasma lulenizing hormone (LH) concentrations in PG $F_{2{\alpha}}$ - treated goats were slightly higher than in controls but mean LH concentrations did not differ between the two treatment groups. 5. Plasma prolactin (PRL) concentrations were suppressed in both GR-1(10 day-treatment group) and GR-2(4 day-treatment group) goats compared to saline controls but mean PRL concentrations remained lower in PG $F_{2{\alpha}}$ treated animals during post-treatment period. 6. The mean number of small and medium follicles present when PG $F_{2{\alpha}}$ was administrated was similar in all does but the increase in number of large follicles($\geq$4mm) tended to be higher in PG $F_{2{\alpha}}$ treated group than controls. These results suggest that concentrations of PG $F_{2{\alpha}}$ and estradiol-17$\beta$ were positively correlared with follicular diameter. We conclude that PG $F_{2{\alpha}}$ treatment stimulates follicular development similarly in both GR-1 and GR-2 group.

Prostaglandin $F_2\alpha$의 투여에 따른 자성 Rat의 혈중 Hormone 함량과 황체의 형태학적 변화 II. Prostaglandin$F_2\alpha$의 투여가 임신 Rat의 황체 구조에 미치는 영향

변명대,조유정,성태수 한국동물번식학회 1992 Reproductive & developmental biology Vol.16 No.1

The present study was carried out to investigate morphologic changes in the corpus luteum of the pregnant rat by electron microscope after administration of prostaglandin F2$\alpha$(PGF2$\alpha$). Pregnant rates were treated with PGF2$\alpha$(1,500$\mu\textrm{g}$/rat) and their corpura lutea were observed morphologically. The results obtained in this study were summarized as follows ; 1. The weight of the ovaries and corpura lutea were decreased slightly at 8~24 hours after PGF2$\alpha$ administratin but no significant differences were observed. 2. The number of corpora lutea and luteal cells decreased slightly at 12~48 hours and 18~24 hours after PGF2$\alpha$ tretment but there were no signifciant differences between control and treatment. 3. The weight of uterus and the unmber of embryo decreased slightly at 96 hours and at 18~96 hours after PGF2$\alpha$ administration but no significant differences were obtained. 4. In the electron microscopic observatons, lipid droplets which are electron dense and appear in the cytoplasm moderately increased in number after PGF2$\alpha$ treatment. The lipid droplets were surrounded by mitochodria and appeared in the autophagic vacuoles. 5. Moderated and high electron dense mitochondria which are round or elongated in shape showed pleomorphism from 3 hours after PGF2$\alpha$ treatment. Destruction of tubular of vesicular cristae was observed at 6 hours after the treatment. Dense body and myelin figures in matrix of mitochondria were also appeared. 6. Well-developed smooth endoplasmic reticulum(sER) showed tubular or vesicular cisternae. A number of whorl membranes containing ribosomes, mitochondria and lipid droplets were observed at 1.5 hour after treatment. sER was abundant in luteal cells at 12 hours were treatment. 7. Well-developed Golgi pparatus appeared obviously 6 hours and more prominently at 12 hours. Those Golgi vesicles were remarkably dilated. 8. Generally, a few rough endoplasmic reticulum (rER) were appeared after treatment and cisternae showed slight dilatation. No differences among the treatments were observed. However, slight dilation of cisternae was observed at 1.5 hours after treatment. 9. Ribosomes composed of free and polyribosomes were abundant before treatment but polyribosomes were appeared at 12 to 24 hours after treatment. 10. Intercellular space were slightly extended at 3 hours and markedly extended at 12 hours. Numerous microvillous protrusions were observed at these times. Membranous multivesicular structures and autophagic vacuoles were also appeared in the intercellular space. 11. At 3 hours after the treatment, autophagic vacuoles appeared in the cytoplasm of the cell. They increased in number with time and were observed to transfer to the intercellular space. Lysosomal dense body appeared in the cytoplasm and the inclusion body was also observed in nucleus at 12 to 24 hours after treatment.

山羊에 있어서 分娩後 姙娠黃體의 退行

邊明大,咸泰守 한국동물생명공학회(구 한국동물번식학회) 1991 Reproductive & developmental biology Vol.15 No.3

山羊에 있어서 分娩後 子宮의 變化

邊明大 한국동물생명공학회(구 한국동물번식학회) 1993 Reproductive & developmental biology Vol.17 No.3

Prostaglandin F_2α의 投與가 姙娠 Rat의 黃體에 미치는 影響

邊明大,李熙碩 慶北大學校 1982 論文集 Vol.34 No.-

These experiments were carried out to investigate the weighs of the ovaries and corpora lutea, number of corpora lutea and luteal cells, weight of uterus and number of embryo, as well as morphological changes in corpus luteum from 30 minutes to 96 hours following a single subcutaneous injection of a luteolytic dose (1500 μg/rat) of prostaglandin F_2α(PG-F_2α) to pregnant rats (day 10 post coitum). Histological changes of corpora lutea in pregnant rats injected with PGF_2α were examined by light microscopy at the respective times. The results obtained in this study were summarized as follows; 1. The weights of the ovaries and corpora lutea were decreased slightly at 18 to 24 hours of PGF_2α administration but no significant differences were observed. 2. The number of corpora lutea and luteal cells decreased slightly at 12 to 48 hours and 18 to 24 hours of PGF_2α injection but there were no significant differences between control and treatment. 3. The weight of uterus and the number of embryo decreased slightly at 96 hours and at 18 to 96 hours of PGF_2α administration but no significant differences were obtained. 4. In the light microscopic observation of corpora lutea, moderately extented blood vessels of central portion, appearance of lipid droplets, cytoplasmic vacuolation and pyknosis in luteal cells were observed at 1.5 hours after PGF_2α injection. These changes were more evident at 24 hours and those findings recovered to nomal at 96 hours of treatment.

유우 Anestrus 의 발생상황에 관한 조사연구

변명대,조헌조 ( M . D . Byun,H . J . Cho ) 한국축산학회 1974 한국축산학회지 Vol.16 No.3

The 1,042 Holstein cows raised in the Kyung-buk area were investigated for studying the incidence of anestrus and abnormalities associated with organic anestrus in dairy cattle. The research of extensive survey was carried out for two years from 1971 to 1972. The results obtained in this study can be summarized as follows: 1. In a study of 1,042 Cattle, 954 cases (40.9%) of preservice and postservice anestrus were recorded in 2328 reproductive cycles. 2. There were 107 cases of organic anestrus, representing 4.5% of total reproductive cycles and 11.1% of all recorded anestrus cases. 3. There were 847 cases of functional anestrus, accounting for 36.3% of total reproductive cycles and 88.7% of all recorded cases of anestrus. 4. There were 9.7% preservice and 26.6% postservice anestrus cases recorded in the category of functional anestrus. 5. Individual organic abnormalities causing or leading to anestrus were; (a) Luteal cysts (41.4%) ; Pyometra (28.9%) ; Follicular cysts (16.8%) ; Persistent corpus luteum (8.4%) ; White heifer disease (2.8%) ; Granulosa cell tumor (1.8%). 6. Pregnancy examination revealed that 21.3% were not pregnant. 7. In 1,042 cattle, total economic losses due to anestrus for a two year period were estimated at ￦3,690,000.

Prostaglandin F₂α의 投與가 韓國在來山羊의 黃體機能에 미치는 影響

邊明大 慶北大學校 1986 農業科學技術硏究所報 Vol.3 No.-

韓國在來 牝山羊에 있어서 prostaglandin F₂α의 投與가 黃?退行, 發精 및 排卵과 聯關하여 內分泌에 미치는 효果를 檢討하기 위하여 發情終了 後 2日齡 의 牝山羊에게 PGF₂α 1,500㎍을 單一 筋肉注射한 후 血漿中 progesterone, estrone, estradiol-17ß, FSH, LH 및 prolactin 의 含量變化를 測定하였다. 本 硏究에서 얻어진 結果는 다음과 같다. 1) 發情終了 後 2日齡의 在來山羊에 PGF₂α 投與 後 血漿中 progesterone 濃度는 3時間에 0.16 ±0.01ng/ml (處理前値, 0.34 ±0.02ng/ml)으로 多少 減少하였으나 12 및 24時間 後에는 增加하는 傾向을 나타내 보였다. 2) 血漿中 estrone濃度는 PGF₂α의 處理에 의하여 有意差는 認定되지 않았다. 3) 血漿中 estrobiol-17ß 濃度는 PGF₂α의 處理에 의하여 12 및 24時間에 前處理値보다 有意하게(P<0.05) 減少하는 傾向을 나타내었다. 4) 血漿中 FSH濃度는 PGF₂α의 投與에 의하여 3時間 後에 急增하였으나 그 이후 FSH濃度의 有意性은 認定되지 않았다. 5) 血漿中 LH濃度는 PGF₂α의 投與에 의하여 多少 上昇하는 傾向을 보였으나 統計的 有意差는 認定되지 않았다. 6) 血漿中 prolactin 濃度는 PGF₂α의 投與에 의하여 處理前値에 比하여 약간 減少하는 傾向을 보였으나 有意差는 認定되지 않았다. The purpose of the present study was to evaluate the effects of injection of prostaglandin F₂α on endocrine events associated with luteolysis, estrus and ovulation of Korean native goats. On day 2 of the ensuing cycle, goats were treated with a single I.M. injection of a synthetic prostaglandin F₂α at a dose of 1,500μg. Blood samples wre collected at O hr prior to administration of PGF₂α and at 3, 6, 12 and 24 hr after PGF₂α administration(IM). Progesterone, estrone, estradiol, FSH, LH and prolactin in plasma were measured by radioimmunoassay. 1. Treatment with PGF₂α resulted in a decline in the concentration of plasma progesterone 0.34 ±0.02ng/ml to 0.16 ±0.01ng/ml(34%) within 3 hr. However, progesterone levels were further increased by 12 and 24 hr after PGF₂α injection. 2. No differences(p<0.05)in estron concentrations were observed between group treated with PGF₂α 3. The mean concentration of estradiol-17βin the PGF₂α-treated goats was reduced(p>0.05) when compared to saline treated controls at 12 and 24 hr after the injection. 4. There was a transient rise in FSH concentration the during first 3 hr after treatment. 5. The concentration of LH was increased gradually from the PGF₂α treatment However, LH levels were not different, respectively. 6. Mean concentration of prolaction in the PGF₂α-treated goats did not from those in the vehicle-treated controls.

H.C.G.를 投與한 産羊에 잇어서 Anti-H.C.G.의 産成에 관한 硏究

邊明大 건국대학교 1978 論文集 Vol.8 No.1

Three goat were injected with H. C. G. to determine the effect of dosage and frequency of injection upon the production of antigonadotropins, The three female goat were treated once weekly with various doses of H. C. G. injected subcutaneously. The results obtained were summarized as follows : 1. Anti-HCG activity appeared by the 2nd week with injection of 500 1u (G), by the 3rd week with 1,000 1u (G), and by th 5 week with the highest dose (2,000 1u, G). 2. Anti-HCG activity in G treated with 2,000 1u of HCG did not reach a peak until about 7th to 9th week but maximum potency was reached in 3 to 7 weeks in goat (G , G) receiving continuous once-weekly injections. 3. Antigonadotrophins were not detected in the sera of untreated goat (control and goat before treatment). The lowest of HCG injected (500 1u per week) brought about a more rapid production and earlier peak activity than did the higher dose level (2,000 1u group). 4. Anti-HCG serum completely inhibited to the activity of as much as 5 1u of HCG. It is concluded that antihormane formation is a serous factor in the clinical use of HCG in animals if minimal physiological doses are not employed.

Prostaglandin F2α의 投與에 따른 雌性 Rat의 血中 Hormone 含量과 黃體의 形態學的 變化 - Ⅱ. Prostaglandin F2α의 投與가 姙娠 Rat의 黃體 構造에 미치는 影響

邊明大,槽裕貞,咸泰守 한국동물생명공학회(구 한국동물번식학회) 1992 Reproductive & developmental biology Vol.16 No.1

한국 재래 산양에 있어서 Prostaglandin F/sub 2α/(PGF/sub 2α/)의 투여가 호르몬 함량 및 난포의 발육에 미치는 영향

변명대,함태수 한국동물생명공학회(구 한국동물번식학회) 2001 Reproductive & developmental biology Vol.25 No.2