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섬유주세포에서 고농도 포도당이 활성산소종의 생성에 미치는 영향
배창범,김재우,Chang Beum Bae,Jae Woo Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.3
Purpose: To investigate the effect of high glucose concentration on the production of reactive oxygen species in cultured human trabecular meshwork cells (HTMCs). Methods: Primarily cultured HTMCs were exposed to low glucose (5 mM) and high glucose (22 mM) concentrations, respectively, for seven days. Cellular survival, as well as nitric oxide (NO) and hydrogen peroxide production, were assessed by measured MTT assay, Griess assay, and o-Dianisidine dihydrochloride assay, respectively. Some cells were co-exposed to N-acetyl cysteine (NAC) to assess the effect of this antioxidant. Results: High glucose concentration increased the survival of cultured HTMCs significantly, with no effect from NAC. High glucose concentration increased the production of NO and hydrogen peroxide, which were abolished by co-exposure with NAC. Conclusions: High glucose concentration increases the production of NO and hydrogen peroxide, which can be abolished by antioxidant in trabecular meshwork cells. J Korean Ophthalmol Soc 2010;51(3):418-422
비스포스포네이트 투여와 관련된 양측성 대퇴골 전자하부 골절 1예의 장기간 추적 경과
이미연,안소연,최용준,김영집,김한결,배창범,안선주,원예연,정윤석 중앙대학교 의과대학 의과학연구소 2012 中央醫大誌 Vol.37 No.3
Bisphosphonates have been used in the treatment of postmenopausal osteoporosis for many years. However, it has been concerned that long-term bisphosphonate therapy may lead to over-suppression of bone remodelling and increase skeletal fragility. Recently, atypical subtrochanteric femoral fractures are increasingly reported in patients on long-term treatment with bisphosphonates. We experienced a case of a 75-year-old woman who had a subtrochanteric femoral fracture after receiving long-term bisphosphonate therapy for osteoporosis. After more than 7 years of taking bisphosphonate, the patient developed left anterior thigh pain that was getting worse. A subtrochanteric insufficiency fracture of the left femur was demonstrated by radiographs. There was focally-increased uptake in both mid-femoral shaft by whole body bone scan. The lesion was recognized as pseudofracture. Bisphosphonate was discontinued and she underwent left intramedullary rod fixation. This patient had been treated with oral calcium, vitamin D, and raloxifene. She recovered without further complication.
배창범,최윤주,이승한,박지윤,김월민,오병철 대한신경과학회 2015 대한신경과학회지 Vol.33 No.1
Lemierre’s syndrome is characterized by anaerobic bacterial infection in the head and neck, causing thrombophlebitis of the jugular vein. This disease is usually associated with a history of pharyngitis. The most common pathogens are Fusobacterium species, particularly Fusobacterium necrophorum. Lemierre’s syndrome is seen most commonly in teenagers and young adults. We present a case report of a 67-year-old man with an atypical clinical manifestation of an uncommon pathogen in Lemierre’s syndrome with epilepsia partialis continua.
김재우,배창범,이정훈 대한안과학회 2009 대한안과학회지 Vol.50 No.10
목적: 고포도당이 섬유주세포의 산화스트레스에 미치는 영향을 알아보고자 하였다. 대상과 방법: 일차배양 섬유주세포를 고농도(25 mM)와 저농도(5 mM)의 포도당이 포함된 배지에 각각 7일간 노출시켰으며 Larginine, DAHP, insulin, 아스코르빈산, sepiapterin에도 노출시켰다. 세포생존과 일산화질소 생성, superoxide 생성을 MTT, Griess, modified cytochrome c assay로 측정하였다. 결과: 고포도당은 섬유주세포의 생존에는 유의한 영향을 미치지 않았으나 일산화질소의 생성을 유의하게 억제하였고 동시에 노출한 DAHP는 일산화질소의 생성을 더욱 억제하였으나 insulin과 sepiapterin은 일산화질소의 생성을 증가시켰다. 고포도당은 superoxide의 생성을 증가시켰으며 insulin과 아스코르빈산, sepiapterin은 superoxide의 생성을 감소시켰다. 결론: 고포도당은 섬유주세포에서 일산화질소의 생성을 억제하는 동시에 superoxide의 생성을 증가시켰다. 고포도당은 섬유주세포에서 산화스트레스를 유발하여 섬유주의 손상과 기능저하를 초래할 수 있을 것이다. Purpose: To investigate the effect of high glucose (HG) on the oxidative stress in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to low glucose (5 mM) and HG (25 mM) for 7 days. Additionally, 1 mM Larginine, 5 mM DAHP, 10 μg/ml insulin, 100 μM L-ascorbic acid, 10, and 100 μM sepiapterin were co-exposed. The cellular survival and nitric oxide (NO) production were assessed by MTT assay and Griess assay, respectively. Superoxide production was measured by modified cytochrome c assay. Results: HG did not affect the survival of cultured HTMC significantly. HG decreased NO production. Co-exposed DAHP decreased but DAHP and insulin increased NO production. In addition, HG increased superoxide production, which was decreased by insulin, L-ascorbic acid, and sepiapterin. Conclusions: HG decreased NO production accompanied with increased superoxide production in HTMC. Thus HG induces oxidative stress in HTMC and may cause cellular dysfunction and damage of the trabecular meshwork.
이민정,안소연,배창범,손영배,정윤석 대한내분비학회 2012 Endocrinology and metabolism Vol.27 No.2
CATCH 22 is a medical acronym for cardiac defects, abnormal faces, thymic hypoplasia, cleft palate, and hypocalcemia, and a variable deletion on chromosome 22. It includes DiGeorge syndrome, conotruncal anomaly face syndrome, and velo-cardio-facial syndrome. It has a prevalence estimated at 1:3,000-1:6,000. Most deletions occur at de novo, but autosomal dominant inheritance is observed in 6-10% of cases. Hormonal disorders are common in patients with CATCH22 syndrome. While hypoparathyroidism was the predominant endocrine disturbance that has been documented in the DiGeorge syndrome, other hormonal defects, such as growth hormone deficiency, hypothyroidism, and hyperthyroidism have been occurred in patients with CATCH22 syndrome. The spectrum of parathyroid gland dysfunction in this syndrome ranges from severe neonatal hypocalcemia to normal parathyroid function. Most patients are usually diagnosed in young age, but a few patients with mild abnormality are presented later in life. We report a case of CATCH22 syndrome with normal parathyroid hormone and calcium level in an adult. The diagnosis of CATCH22syndrome was confirmed by fluorescence in situ hybridization analysis.
말초신경초음파검사를 이용한 흔한 신경포착부위 검사; 단면적기준치와 정상변이
석정임,이상법,배창범 대한신경과학회 2015 대한신경과학회지 Vol.33 No.1
Background: Neuromuscular ultrasound has emerged over the last decade as a useful tool for diagnosing peripheral neuropathy. Because nerve enlargement is the most important diagnostic marker of an abnormal nerve, quantification of nerve size is essential. Methods: We included 80 healthy volunteers aged 21–60 years. The nerve cross-sectional area (CSA) was measured in all participants at the following common compressive sites: bilateral median nerve at the carpal tunnel and forearm, ulnar nerve at the wrist and medial epicondyle, radial nerve at the spiral groove, peroneal nerve at the fibular head and popliteal fossa, and tibial nerve at the popliteal fossa. Anatomical variants were also evaluated, including a bifid median nerve and persistent median artery. Results: The CSAs were 9.58±1.55, 6.87±1.61, 4.72±0.91, 6.64±1.33, 6.48±1.68, 12.35±3.55, and 26.98±6.92 mm2 (mean±SD) for the median nerve at the carpal tunnel and forearm, ulnar nerve at the wrist and medial epicondyle, radial nerve at the spiral groove, peroneal nerve at the fibular head, and tibial nerve at the popliteal fossa, respectively. The nerve CSA was significantly larger in men than in women and was correlated with body mass index, weight, and height. A bifid median nerve and persistent median artery were seen in 12 (7.5%) and 4 (2.5%) of 160 hands, respectively. Conclusions: The information produced in this study can serve as reference data when evaluating these nerve sites using ultrasound.