Cationic Oligopeptide-Functionalized Mitochondria Targeting Sequence Show Mitochondria Targeting and Anticancer Activity

배윤희,주찬양,김구영,고경수,허강무,한진,최준식 한국고분자학회 2019 Macromolecular Research Vol.27 No.11

Mitochondrial drug delivery systems require development of highly selective mitochondria-targeting carriers. In this study, we report that mitochondria targeting sequence (MTS)-hybrid cationic oligopeptide, MTS-H3R9, shows the dual role of a mitochondria targeting vector along with anticancer effect for cancer therapy. In cytotoxicity assays, MTS-H3R9 was shown to be more effective than MTS. MTS-H3R9 showed significant cell penetration and internalization activity compared to that of MTS along with more efficient escape from lysosome to the cytosol. We showed efficient targeting of MTS-H3R9 to mitochondria in HeLa cell line. Furthermore, we exhibited anticancer agent properties that mitochondrial-accumulated MTS-H3R9 caused cell death by reactive oxygen species generation and loss of mitochondrial membrane potential. MTS-H3R9 exhibited dramatically increased anticancer activity in 3D spheroids as well as in a 2D culture model. We demonstrated that MTS-H3R9 provides dual potentials both as a vehicle for targeted delivery and as a cancer treatment agent for therapeutic applications.

Effect of Motor Control Training with Strengthening Exercises on Pain and Muscle Strength of Patients with Shoulder Impingement Syndrome

배윤희,이규창 대한물리치료학회 2011 대한물리치료학회지 Vol.23 No.6

Purpose: The purpose of this study was to investigate the effect of movement training based on motor control theory on pain and average power of muscles in patients with shoulder impingement syndrome and to develop more effective training methods. Methods: We studied 35 patients with shoulder impingement syndrome. Patients were randomly assigned to an experimental group or a control group according to the intervention. The therapeutic modalities such as superficial heat,deep heat, and electronic stimulus, and motor control training with strengthening exercises were applied to the experimental group and therapeutic modalities only were applied to the control group. All interventions were done 3 times a week for 4 weeks. Before the intervention and again after the 4 weeks, we measured pain utilizing a visual analog scale. We measured average power using isokinetic equipment. Results: After application of the intervention, pain significantly decreased in both the experimental group and the control group. There was a significant difference between the extent of the decrease between experimental and control groups. After the intervention, the average power between the two groups were significant at an angle of 60°/sec and 180°/sec for external rotator and internal rotator muscles. They also showed significant improvement in all variables post‐intervention compared to pre‐intervention. Conclusion: This study indicates that there is an effect in giving appropriate motor control training to patients with shoulder impingement syndrome. It is assumed that there will be a need for more surveys on various variables for motor control training from now on.

블렌디드 수업을 위한 학습 오리엔테이션 개발 및 학습자 인식 연구

배윤희 인문사회 21 2022 인문사회 21 Vol.13 No.6

블렌디드 수업을 위한 학습 오리엔테이션 개발 및 학습자 인식 연구배 윤 희* 연구 목적: 이 연구는 블렌디드 수업을 위한 학습 오리엔테이션의 효과성을 살펴보고 시사점을 제공하는데 목적이 있다. 연구 방법: 이를 위해 학습 오리엔테이션 콘텐츠를 개발하고, 두 학기 동안 사전 조사를 실시하여 2회 모두 응답한 학습자 51명을 대상으로 인식 변화를 비교・분석하였다. 연구 내용: 연구 결과, 학습 오리엔테이션 실시는 ‘학습목표 인지’, ‘수업방식 인지’, ‘수업방식 이해정도’, ‘평가방식 인지’, ‘수업방식 이해도’에 도움을 주며, 오리엔테이션 실시 전・후를 비교한 결과 ‘전반적 사전이해도’, ‘수업방식 이해도’에 도움을 주었다. 마지막으로 회귀분석 결과 ‘온라인 학습능숙도’가 높을수록 ‘수업방식 이해도’에 긍정적인 영향을 미치는 것으로 나타났다. 결론 및 제언: 본 연구에서는 블렌디드 수업 오리엔테이션 실시 효과를 검증하여 블렌디드 수업의 효과적인 운영을 위한 수업 요소로서의 의미를 규명하였다는 점에서 의의가 있다. 핵심어: 블렌디드 수업, 학습 오리엔테이션, 온라인 콘텐츠, 사전 조사, 학습자 인식 □ 접수일: 2022년 10월 4일, 수정일: 2022년 11월 11일, 게재확정일: 2022년 12월 20일* 한국공학대학교 메타버시티혁신센터 책임교수(Professor, Tech Univ. of Korea, Email: blancagloria@tukorea.ac.kr) A Study on the Development and Perception ofStudent Orientation for Blended LearningYunhee Bae Abstract: The purpose of this study was to explore the value of a student orientation for blended learning by designing and implementing one for university learners and examining its educational effects. To do so, we designed and implemented the student orientation content in the context of blended learning. The pre-survey was conducted twice for 2 semesters with 51 students to find out how they perceive blended learning. First, conducting the student orientation helps to understand ‘perceptions of instructional objectives’, ‘perceptions of course modalities’, ‘perceptions of evaluation methods’, and ‘course modalities understanding’. Second, comparing before and after the implementation of the student orientation group, it helps improve 'overall pre-understanding' and 'course modalities understanding'. Further, the higher the online skillfulness, the more positive the student orientation with the course modalities. We further present suggestions for implementing student orientation in blended learning based on the study results. Key Words: Blended Learning, Student Orientation, Online Content, Pre-survey, Student Perception

Smac Gene Delivery by the Glycol Chitosan with Low Molecular Weight Polyethylenimine Induces Apoptosis of Cancer Cells for Combination Therapy with Etoposide

배윤희,이영화,고경수,한진,최준식 한국고분자학회 2019 Macromolecular Research Vol.27 No.10

We developed polymer-based nanoparticle carriers for gene delivery, glycol chitosan (GC)-methyl acrylate-low molecular weight polyethylenimine (800Da PEI), GMP, and investigated their ability to deliver second mitochondria-activation of caspase (Smac) gene into cancer cell lines. We analyzed cytotoxicity level and gene transfection efficiency in GBL-14, U373-MG, MDA-MB-231, and dermal fibroblasts (HDF) cell lines. GMP exhibited significantly lower cytotoxicity level, as compared with PEI25KD. Also, it showed high transfection efficiency in these cell lines. We treated these cells with a complex of GMP and Smac, and investigated their facilitated cellular uptake by flow cytometry. Also, analyses using cell cycle distribution, Annexin V staining, JC-1, and caspase-3 activity, indicated that apoptosis was induced by GMP/ FLAG-Smac combined with etoposide in MDA-MB-231 cell line. This study demonstrates that combining the GMP/FLAG-Smac complex with chemotherapeutic agents could potentially be an effective approach for cancer therapy.

산업연계 교육 자문 위원을 활용한 IC-PBL 비교과 프로그램 사례

배윤희 한국인적자원개발학회 2019 한국인적자원개발학회 학술연구발표회 발표논문집 Vol.2019 No.06

블렌디드 수업 요소가 수업 만족도에 미치는 영향

배윤희,원용호 한국교육공학회 2023 교육공학연구 Vol.39 No.2

To confirm the effect of blended learning course elements on course satisfaction, this study examined (1) how the online learning environment, online content, offline course, and student interactions affect course satisfaction; and (2) how course design consultation influences blended course elements and satisfaction. A survey was administered through a learning management system (LMS), and analyses were performed on 253 university students. A mediating effect test with bootstrapping was employed, and all analyses were performed using R version 4.1.1. Results showed that, first, blended learning elements affected interactions among students and that online content and offline courses affected their satisfaction. Second, online content and offline courses, excluding the online learning environment, partially mediate the relation between student interaction and satisfaction. Third, a significant difference was observed in blended learning elements and interaction depending on whether course design consultations were conducted. These findings suggest that the design of blended learning courses must prioritize fostering student interactions in both the online and offline phases.

메타버스 활용 수업 운영 및 학습자 인식 연구 : 예비대학 물리학 수업을 대상으로

배윤희,신윤희,이수정 한국콘텐츠학회 2022 한국콘텐츠학회논문지 Vol.22 No.12

Educational use cases of metaverse are rapidly increasing, it is needed to develop and operate based on specific instructional strategies for sustainable use. In this study, to explore the value of a metaverse-based university curriculum, a physics course using metaverse technology was designed and implemented for preliminary university learners and the educational effects of the course were also analyzed. For this purpose, we designed the course content reflecting the scenario-based metaverse content develop principle for two chapters and explored the learner's perceptions after its implementation. The perception survey was conducted with 134 students and the questionnaire on overall user satisfaction was conducted twice. The result of the analysis of this study showed that the higher the interest and recommendation, the higher the possibility of continued interest in the metaverse. In addition, the higher the psychosocial satisfaction and the perception of use, the more positive the educational satisfaction of the students with the metaverse-based course. The results of this study are significant in that we designed metaverse content reflecting immersion and interaction elements, and explored the perceptions of the learners along with the psychosocial aspects. 메타버스의 교육적 활용사례가 급증하고 있기 때문에, 지속가능한 활용을 위하여 구체적인 교수전략에 기반한 개발 및 운영이 요구되는 시점이다. 본 연구에서는 메타버스 기반 수업을 설계 및 운영하고, 예비대학 학습자를 대상으로 교육 효과를 분석하여 대학교육에서 메타버스 기반 교육적 활용 가능성을 조망하고자 하였다. 이를 위해 시나리오 기반 메타버스 콘텐츠 설계 원리를 반영하여 2개 차시에 해당하는 콘텐츠를 개발하고 수업 운영 후 학습자의 인식을 탐색하였다. 인식 조사를 위하여 수강생 134명을 대상으로 전반적 사용 만족도에 대한 설문을 시간차를 두고 총 2회에 걸쳐 수행하였다. 주요 연구 분석 결과로는 흥미유발과 추천여부가 높을수록 메타버스에 대한 흥미가 지속될 가능성이 높았으며, 심리·사회적 만족도와 사용 인식도가 높을수록 메타버스 활용 수업에 대한 교육 만족도에 긍정적인 영향을 보였다. 본 연구 결과는 지속가능한 메타버스 활용 교육을 위하여 몰입감 및 상호작용 요소를 반영한 메타버스 콘텐츠를 설계하고, 이에 대한 학습자의 인식을 심리·사회적 측면과 함께 탐색했다는 점에서 의의가 있다.

포스트 코로나 시대의 블렌디드 수업 평가준거 타당성 연구: 공학계열 대학을 중심으로

배윤희,원용호,Bae, Yun-hee,Won, Yongho 한국공학교육학회 2022 공학교육연구 Vol.25 No.5

In the post-Corona era, blended learning will be one of the most important instructional strategies for successful education. The purpose of this study is to examine reliability and validity of the instrument developed in the instructional aspect so that a successful blended learning can take place. This instrument consists of 31 items to evaluate class operation, online learning environment, online contents, offline class, interaction and overall satisfaction. For this study, a survey was conducted in LMS and the responses of 164 students were used for analysis. Confirmatory factor analysis was used to evaluate validation of this instrument and this analysis was run in R studio. As a result of CFA, the standardized factor loadings of all items were 0.930~0.754 and the reliability and validity of all constructs were adequate. The results of this instrument enable universities to manage the quality of their classes and instructors can use them as self-checklist to improve future classes in terms of instructional points. Finally, this instrument can be used in a variety of learner-centered learning environments.

보편적 학습설계(UDL)를 통한 교실 내 인성교육의 실천

배윤희,안미리 한국교육공학회 2015 한국교육공학회 학술대회발표자료집 Vol.2015 No.2

지난 15년간 추진되어온 ‘모두를 위한 교육’은 학습권 보장을 강조하였으나, The 2030 Agenda에서 제시된 17개 지속가능한 목표 중 4번은 양질의 교육과 포용적인 교육을 지향한다. 포용적 가치는 21세기 교육현장의 핵심 가치로서 inclusive한 교육은 인성교육의 실천적 전략중 하나이다. 세계 최초로 시행된 ‘인성교육진흥법’은 우리나라에서 좀 더 체계적인 인성교육을 가능하게 하나 창의적ㆍ체험 활동과 교과목 중심 교육으로 강조되고 있다. 인성교육은 분리된 교육활동이 아니라 모든 교수-학습활동에 흡수되어 제공되어야 하고, 교사가 핵심 가치를 모델링하며, 학생들의 실천을 가이드 해야 한다. 교실 내 인성 교육을 실천하기 위한 방안 중 하나는 먼저 배려와 포용, 이해를 기본으로 하는 학습 환경이 필수적이다. 교실 내 문화나 언어의 차이, 학생들의 배경지식과 개별적 능력의 차이를 포용하는 ‘안전망’ 안에서 인성교육이 가능하다. 보편적 학습설계(Universal Design for Learning: UDL)는 학생들의 다양한 능력과 학습방식, 배경지식과 학습 준비도, 문화 등을 배려하여 모든 학생에게 평등한 ‘학습’의 기회를 제공하고자 한다. 따라서 본 연구에서는 UDL 기반 대안적 교수전략을 활용한 인성교육 실천방안을 구현할 수 있다. 모든 학생들이 배려되는 보편적 교실 내 학습활동은 인성교육을 위한 가장 현실적인 실천 전략이다.

PBL 수업 요소가 수업 만족도에 미치는 영향: 공학계열 대학 중심으로

배윤희,원용호 한국방송통신대학교 미래원격교육연구원 2023 평생학습사회 Vol.19 No.3

This study used a structural equation model and 1,492 survey respondents to examine the relationship between class goals and expectations, team activities, facilitation, evaluation, learning volume and difficulty, learning outcomes, and overall satisfaction in PBL classes. The main research results are as follows: (1) Class goals and expectations, as well as facilitation, did not affect learning outcomes; however, team activity, evaluation, as well as learning volume and difficulty, had a positive effect. (2) Learning outcomes partially mediated the relationship between team activity as well as learning volume and difficulty on overall satisfaction. The results of this study provide implications for consideration in the development and operation of PBL classes.