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White spot syndrome virus (WSSV)의 VP28에 대한 단클론 항체 생산
방지형 ( Ji-hyeong Bang ),김위식 ( Wi-sik Kim ),김춘섭 ( Choon-sup Kim ),김종오 ( Jong-oh Kim ),오명주 ( Myung-joo Oh ) 한국어병학회 2019 한국어병학회지 Vol.32 No.1
We developed and subsequently characterized mouse monoclonal antibodies (MAbs) against recombinant VP28 structural protein (rVP28) of white spot syndrome virus (WSSV). We established six hybridoma clones secreting MAbs against rVP28: 15A11, 20G6, 31H2, 34H6, 38D1 and 43A1. All six MAbs recognized the 25 kDa of protein in gill homogenates of WSSV-infected shrimp by western blot analysis, while no reactivity was observed in gill homogenates of normal shrimp. Moreover, high enzyme-linked immunosorbent assay (ELISA) optical density (OD) values (0.8-2.68) were observed in the hemolymphs from WSSV-infected shrimp, while low OD values (less than 0.24) were recorded in the hemolymphs from normal shrimp, by using these six MAbs produced in this study. These results suggest that these six MAbs are useful for the detection of WSSV.
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넙치 치어에서의 바이러스성출혈성 패혈증 감염에 따른 체내 바이러스분포 및 초기면역유전자 발현 비교
김수진,방지형,오명주 한국어병학회 2023 한국어병학회지 Vol.36 No.1
Viral hemorrhagic septicemia virus (VHSV) is the etiological agent of viral hemorrhagic septicemia (VHS), which is one of the most serious viral diseases affecting cultured olive flounder (Paralichthys olivaceus) in Korea. Mortality due to VHSV infection in olive flounder appears in the cold-water season at 8 to 15˚C. Among factors contributing to the onset of VHS epizootics in olive flounder, the water temperature plays essential role in regulation of VHS outbreaks. In order to estimate the VHSV infectivity and distribution, we performed cell culture and qRT-PCR in flounder target organ after VHSV infection. VHSV titration and distribution were similar between spleen and kidney. The different differential expression kinetics of four immune-related genes in spleen and kidney were observed in this study. Perforin was immediately up-regulated and then continuously expressed within 48h in both of spleen and kidney. Mx gene showed the highest immune-related gene expression in this study. IL-8 and ISG-15 were expressed in spleen. It was peaked at 15h and then gradually decreased, whereas these were not relatively expressed in kidney. Our study showed that the immune response in host was immediately activated, further study needs to interpret the interaction between host and pathogen for their survival.
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