Spontaneous Nanoparticle Formation From a Fluorescent Nucleoside Analogue

방은경,문도현,김병현 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.8

A fluorescent nucleoside analogue, ^AC, featuring two non-complementary nucleobases linked through an ethynyl group, was synthesized. The extended π-conjugation imparts ^AC with red-shifted absorbance (relative to adenine and cytosine) and pale-blue fluorescence. It spontaneously forms nanoparticles, which exhibit considerably enhanced fluorescence, without the help of any additional stabilizing agent. The DMSO/water ratio was an important factor influencing the construction of the NPs. X-ray crystallography confirmed the structure of ^AC; dynamic light scattering and scanning electron microscopy confirmed the existence of the nanoparticles.

치근활택술과 치은박리소파술 후 치아동요도 변화에 관한 연구

방은경,채중규,김종관,조규성,Pang, Eun-Kyoung,Chai, Jung-Kiu,Kim, Chong-Kwan,Cho, Kyoo-Sung 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.4

Tooth mobility may be the decisive factor that determines whether dental treatment of any kind is undertaken. Although tooth mobility in isolation says little in itself, the finding of increased tooth mobility is of both diagnostic and prognostic importance. Only the detection of an increase or decrease in mobility makes an evaluation possible. Thus prior to treatment, we must understand the pathologic process causing the observed the tooth mobility and decide whether the pattern and degree of observed tooth mobility is reversible or irreversible. And then it must be decided whether retention and treatment or extraction and replacement. The purpose of this study was to compare tooth mobility at different time period during root planing and flap operation and to relate changes in mobility to each treatment method. Twenty-one patients (287 teeth) with chronic adult periodontitis were treated with root planing(control group) and flap operation(experimental group), and each group was divided 3 subgroups based upon initial probing pocket depth (1-3mm, 4-6mm, 7mm and more). Tooth mobility was measured with $Periotest^{(R)}$ at the day of operation, 4 days, 1 week, 2 weeks, 3 weeks, 4 weeks, 8 weeks, 12 weeks after each treatment. Tooth mobility, attachment loss, radiographic bone loss, and bleeding on probing were measured at the day of operation, 4 weeks, 8 weeks and 12 weeks after treatment. 1. In group initial probing depth was 1-3mm, tooth mobility had no significant difference after root planing and flap operation. 2 . In group initial probing depth was 4-6mm, 7mm and more, tooth mobility had decreased in 12 weeks after root planing(p<0.01). And the mobility had increased after flap operation(p<0.01) and was at peak in 1 week, and decreased at initial level in 4 weeks, below the initial level in 12 weeks(p<0.01). 3. In 1 week, significant difference in tooth mobility between control and experimental group was found(p<0.01) but, in 12 weeks no difference between two groups was found. 4. Change of immediate tooth mobility after treatment was more larger in deep pocket than in shallow one. In group with the same probing pocket depth, the change of tooth mobility in molar group was greater than that of premolar group. 5. Tooth mobility before treatment was more strongly correlated with radiographic bone loss (r=0.5325) than probing depth, attachment loss and bleeding on probing, in 12 weeks after treatment, was more strongly correlated with attachment loss($r^2$=0.4761) than probing depth and bleeding on probing. Evaluation of the treatment effect and the prognosis after root planing and flap operation were meaningful on tooth initial probing depth 4mm and more. After flap operation, evaluation of the prognosis should be performed at least in 4 weeks and in 12 weeks after treatment, no difference in tooth mobility between two groups was observed. Radiographic bone loss and attachment loss were good clinical indicators to evaluate tooth mobility.

유아 음률활동 지도의 실제

방은경 국립특수교육원 2004 자격연수 Vol.- No.6

상악 전치부 심미를 위한 치주성형술

방은경 이화여자대학교 임상치의학대학원 2009 개원 6주년 기념 학술강연회 Vol. No.

치주치료의 주된 목표는 환자의 일생을 통하여 건강하고 편안하며 기능적인 치열과 치주조직을 유지하는 것이다. 이 목적을 위한 치료가 이뤄지지만 이 목적은 심미성을 고려하지 않고서는 더 이상 받아들여 질 수 없으며 최근에는 치료목표의 한 부분으로써 심미성이 크게 받아들여지고 있어 치주낭을 제거하기 위해 시행되는 치주 수술 뿐 아니라 심미성 회복을 목적으로 하는 치주 수술이 빈번히 이루어지고 있다. 이처럼 심미성은 갈수록 치과계에서 그 중요성을 더해가며 치주 성형술은 그 술식 자체로써나 수복 치료 전 처치로써 치주 치료에서 중요한 분야로 자리잡게 되었다. 이와 같이 심미성은 치주 성형술 및 재건술의 바탕이 되며, 특히 상악 전치부에서 심미성은 필수 고려 요소이다. 상악 전치부의 심미성을 떨어뜨리는 요소로는 치은증식이나 치은퇴축에 의한 치은변연의 위치 변화와 그에 따른 치근면 노출 치간유두 소실로 인한 black triangle, gummy smile, 치조융선의 결손, 정중 이개 등을 들 수 있다. 1988년 Miller는 치주조직의 해부학적, 발생학적, 외상성 또는 세균에 의한 질환에 의한 결손을 예방하고 수정해주는 모든 술식을 치주성형수술이라고 명명하였으며, 치근피개술(root coverage), 기능적, 심미적 치관 연장술(crown lengthening procedure), 치조제 보존술(ridge preservation), 치간유두 재건술(reconstruction of papilla), 치간유두 보존술(maintenance of inter-dental papilla), 임플란트 주위의 심미적 연조직 수술 및 교정 치료 목적의 외과적 노출(surgical opening) 등이 여기에 포함된다. 이러한 치주성형수술을 통하여 비심미적인 전치부 치은의 적절한 심미성을 회복해 줄 수 있는데 그 방법은 크게 절제 술식과 재생 술식으로 나눌 수 있다. 절제술에는 기능적, 심미적 치관 연장술, 약물 등에 의한 치은 증식의 수정을 위한 치은절제술 및 멜라닌 색소 제거술이 포함되며 재생 술식으로는 치근 피개, 치간유두 재건술, 치조제 증대술 등이 포함된다. 본 발표에서는 상악 전치부에서 심미성 증진을 위한 치료 술식들의 선택에 대하여 논의하고자 한다.

내과 검사삭감율 감소

방은경,박인선 한국의료QA학회 2009 가을학술대회 Vol.2009 No.2

Current Research Topics : Current researches on allogenic block bone graft

방은경 ( E. K. Pang ) 대한치주과학회 2009 대한치주과학회 학술대회자료집 Vol.2009 No.1

Symposium : Clinical application of bone substitutes

방은경 ( Eun Kyoung Pang ) 대한치주과학회 2007 대한치주과학회 학술대회자료집 Vol.2007 No.2

Root resection of periodontally compromised root of molar

이선경,방은경 대한치주과학회 2007 대한치주과학회 학술대회자료집 Vol.2007 No.1

Effects of enamel matrix derivatives on the proliferation and the release of growth factors of human periodontal ligament cells

정겨운,방은경,Jung, Gyu-Un,Pang, Eun-Kyoung The Korean Academy of Prosthodonitics 2016 대한치과보철학회지 Vol.54 No.3

목적: 치주조직재생을 위해서는 치주인대세포의 증식 및 이주를 촉진시키는 것이 필수적이나 현재까지 이것을 만족시키는 재생술식은 없다. 최근 법랑기질유도체(enamel matrix derivatives)가 치주조직 재생 술식에 적용되고 있으나 그 기전에 대해서는 완전히 알려지지 않았다. 따라서 본 연구의 목적은 법랑기질유도체가 치주인대세포의 증식과 성장인자 발현에 미치는 영향에 대하여 알아보고자 함이다. 재료 및 방법: 건강한 성인의 제 3 대구치로부터 치주인대세포를 추출한 후, 법랑기질유도체(Emdogain (Biora, Malmo, Sweden))의 농도가 각각 0, 12.5, 25, 50, 100, and $200{\mu}g/mL$인 배지에서 배양시켰다. 치주인대 세포증식과 알칼리성 인산분해효소(alkaline phosphatase) 활성도를 측정하고, 발현되는 성장인자를 평가하였다. 결과: 치주인대세포의 세포증식은 $25{\mu}g/mL$이상의 농도의 법랑기질유도체를 첨가한 군에서, 알칼리성 인산분해효소 활성도는 $50{\mu}g/mL$의 법랑기질유도체를 첨가한 군에서 각각 유의하게 증가하였고, $50{\mu}g/mL$의 법랑기질유도체를 첨가한 군에서 치주인대세포의 VEGF (vascular endothelial growth factor)와 TGF(transforming growth factor)-${\beta}$의 발현이 유의하게 증가하였다. 결론: 법랑기질 유도체는 인간 치주인대세포의 세포증식과 알칼리성 인산분해효소 활성을 증진시키고, VEGF와 TGF-${\beta}$등 성장인자의 발현을 촉진함으로써 치주조직 재생에 기여할 수 있을 것이다. Purpose: Stimulating the proliferation and migration of periodontal ligament cells (PDLCs) has become the main goal of periodontal regeneration. To accomplish this goal, regeneration procedures have been developed, but results have not been predictable. Recently, tissue engineering using enamel matrix derivatives (EMDs) and growth factors has been applied to periodontal regeneration; however, the mechanism of EMDs is largely unknown. The aim of this study was to investigate the effects of EMDs on the proliferation and release of growth factors from PDLCs. Materials and methods: Human PDLCs were removed from individually extracted 3rd molars of healthy young adults, and cultured in the media containing EMDs (Emdogain, Biora, Malmo, Sweden) at concentration of 0, 12.5, 25, 50, 100, and $200{\mu}g/mL$ each. Cell proliferation and ALP (alkaline phosphatase) activity were measured. The evaluation of growth factors released by PDLCs was also performed by one-way analysis of variance (ANOVA) and Bonferroni's multiple comparison test. Results: Significantly increased proliferation and ALP activity were observed in PDLCs treated with over $25{\mu}g/mL$ and $50{\mu}g/mL$ EMDs, respectively. Additionally, treatment of PDLCs with $50{\mu}g/mL$ resulted in significantly increased release of vascular endothelial growth factor (VEGF) and transforming growth factor $(TGF)-{\beta}$ after 24 h and 48 h, respectively. Conclusion: EMDs enhance the proliferation and ALP activity of PDLCs, and promote the release of growth factors, including VEGF and $TGF-{\beta}$, from PDLCs. Therefore EMDs could be one of the effective methods for periodontal regeneration.

Anterior maxillary defect reconstruction with a staged bilateral rotated palatal graft

정겨운,방은경,박창주 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.3

Purpose: In the anterior maxilla, hard and soft tissue augmentations are sometimes requiredto meet esthetic and functional demands. In such cases, primary soft tissue closureafter bone grafting procedures is indispensable for a successful outcome. This report describesa simple method for soft tissue coverage of a guided bone regeneration (GBR) siteusing the double-rotated palatal subepithelial connective tissue graft (RPSCTG) techniquefor a maxillary anterior defect. Methods: We present a 60-year-old man with a defect in the anterior maxilla requiringhard and soft tissue augmentations. The bone graft materials were filled above the alveolardefect and a titanium-reinforced nonresorbable membrane was placed to cover the graftmaterials. We used the RPSCTG technique to achieve primary soft tissue closure over thegraft materials and the barrier membrane. Additional soft tissue augmentation using acontralateral RPSCTG and membrane removal were simultaneously performed 7 weeks afterthe stage 1 surgery to establish more abundant soft tissue architecture. Results: Flap necrosis occurred after the stage 1 surgery. Signs of infection or suppurationwere not observed in the donor or recipient sites after the stage 2 surgery. These proceduresenhanced the alveolar ridge volume, increased the amount of keratinized tissue, andimproved the esthetic profile for restorative treatment. Conclusions: The use of RPSCTG could assist the soft tissue closure of the GBR sites becauseit provides sufficient soft tissue thickness, an ample vascular supply, protection ofanatomical structures, and patient comfort. The treatment outcome was acceptable, despitemembrane exposure, and the RPSCTG allowed for vitalization and harmonization withthe recipient tissue.