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RISS 인기검색어
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- 저자 : 박환준(Whan-Jun Park) (검색결과 2 건)
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박환준(Whan-Jun Park),좌미경(Mi-Kyung Jwa),현선희(Sun-Hee Hyun),임상빈(Sangbin Lim),송대진(Dae-Jin Song) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.10
Raw oysters were treated at 10℃ and 22℃/350 MPa/15 min, and microbial counts and quality were measured during storage of 14 days at 10℃. Total viable cell count (TVCC) in untreated oyster increased greatly during storage from starting inoculum of 1.6×10<SUP>2</SUP> CFU/mL, and reached to 5.6×10<SUP>4</SUP> CFU/mL after 4 days of storage. TVCC of the pressure-treated was about 10<SUP>1</SUP> CFU/mL right after high hydrostatic pressure treatment and increased slowly during storage, and about 10<SUP>3</SUP> CFU/mL even after 7 days of storage. Lactic acid bacteria count (LABC) in the untreated was increased greatly during storage from starting inoculum of 3.3×10<SUP>3</SUP> CFU/mL at 3 days of storage, and 7.2×10<SUP>4</SUP> CFU/mL after 4 days of storage. LABC in the pressure-treated was detected only after 5 days of storage, and about 10<SUP>2</SUP> CFU/mL after 8 days of storage. The pH of the untreated was 6.19 and decreased gradually during storage, and 5.83 after 4 days of storage. The pH of the pressure-treated showed little change during storage, and 6.07, 6.03 and 5.82 after storage of 4, 8 and 14 days, respectively. Volatile basic nitrogen (VBN) in the untreated was 16.8 mg%, and maintained almost constant until 1 day of storage, and then increased suddenly, and 30.1 mg% after 4 days of storage. VBN of the pressure-treated stayed unchanged during storage, and about 20 and 23 mg% even after 4 and 8 days of storage, respectively. Hunter L<SUP>*</SUP>, a<SUP>*</SUP> and b<SUP>*</SUP> values were increased until 2 days of storage and then showed no change during storage. Demerit score was the lowest in the thawed raw oyster, and then in the increasing order of the pressure-treated(4 day and 8 day storage) and the untreated (4 day storage).
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생굴 중 Vibrio parahaemolyticus와 Escherichia coli의 초고압 살균
박환준(Whan-Jun Park),좌미경(Mi-Kyung Jwa),현선희(Sun-Hee Hyun),임상빈(Sangbin Lim),송대진(Dae-Jin Song) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.7
V. parahaemolyticus와 E. coli를 접종한 생굴을 대상으로 초고압처리 조건을 달리하여 살균효과를 측정하였다. 처리조건에 따른 V. parahaemolyticus의 균수 변화를 보면, 무처리한 굴의 초기 배양균수는 3.8×10? CFU/mL이었는데,22℃/10분에서 100~300 MPa로 처리한 결과 처리압력의 증가에 따라 감소하였으며 200 MPa 이상의 처리로 완전히 사멸되었다. 22℃/150 MPa에서 5~30분으로 처리한 결과 처리시간의 증가에 따라 지속적으로 감소하였다가 25분 이상처리하였을 때 모두 사멸되었다. 100 MPa에서 0, 10℃로 처리한 결과 동일 처리압력과 처리시간에서 처리온도가 낮을 수록 미생물의 사멸율은 높았으며, 100 MPa/0℃와 10℃에서 각각 20분과 25분 처리하였을 때 완전히 사멸되었다. 처리조건에 따른 E. coli의 균수변화를 보면, 무처리한 굴의 초기 배양균수는 4.0×107 CFU/mL이었는데, 22℃/15분에서 150~400 MPa로 처리한 결과 처리압력 250 MPa까지는 거의 변화가 없었으나 그 이상의 압력에서는 급격히 감소하였으며 375 MPa 이상에서는 완전히 사멸되었다. 300 MPa/15분에서 0, 10, 22℃로 처리한 결과 동일한 처리압력과 처리시간에서 처리온도가 낮을수록 살균효과가 더 높았다. 10℃/350 MPa에서 5~25분으로 처리한 결과 처리시간의 증가에 따라 균수가 급격히 감소하였으며, 350 MPa에서 5분처리로 약 4 log cycle 감소하였고 15분 이상의 처리로 검출한계 이하였다. Raw oyster (Crassostrea gigas) was inoculated with Vibrio parahaemolyticus and Escherichia coli, treated with high hydrostatic pressure and evaluated for microbial counts. Cell death of V. parahaemolyticus (Vp) increased with the increase of applied pressure. Vp starting inoculum of 3.8×10? CFU/mL was totally eliminated after exposure to 200 MPa for 10 min at 22℃. Viable cell of Vp decreased with the increase in treatment time and dropped below the detection limit with treatment for 25 min at 22℃/150 MPa. The number of Vp decreased with the decrease of applied temperature at the same treatment pressure and time. Vp was killed by treatment of 0℃ and 10℃ for 20 and 25 min at 100 MPa, respectively. For E. coli, there was an initial lag up to 250 MPa followed by a rapid decline. Treatment at 325 MPa/22℃ for 15 min caused 5-log reduction, while that at 375 MPa resulted in total reduction of starting inoculum of 4.0×107 CFU/mL. Lower treatment temperature showed higher killing effect of E. coli at the same treatment pressure and time. Viable cell of E. coli decreased with the increase in treatment time, and 4-log reduction was achieved with treatment of 5 min at 10℃/350 MPa and then total reduction was achieved after treatment of 15 min. Higher pressure, lower temperature and longer time were more effective in sterilizing V. parahaemolyticus and E. coli.
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