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咽喉疾患에 應用되는 必用方甘桔湯 및 加味必用方甘桔湯의 效能에 關한 實驗的 硏究
박항기,채병윤,Park, Hang-Gi,Chae, Byeong-Yun 대한한방안이비인후피부과학회 1988 한방안이비인후피부과학회지 Vol.1 No.1
According to oriental clinic effect findings hitherto, Pilyongbangkamgil-Tang has been curative effects on chronic pharnygitis, acute tensillitis and angina. Auther tried to make clear the anti-inflammatory effect on rat paw which has become edema formation by $5\%$ of acetic acid/saline solution, and the analgesic effect on mouse thorough method of acetic acid because the medicine decoction of Kamipilyongbangkamgil-Tang, Pilyongbangkamgil-Tang, and the extracted powder of Pilyongbangkamgil-Tang are administered to rat and mouse. In present report, anti-inflammatory, analgesic effect of Pilyongbangkamkil-Tang, the extracted powder of the above prescription and Kamipilyongbangkamgil-Tang were estimated by the above test. The results are summarized as follows. 1. Kamipilyongbangkamgil-Tang (liquid), Pilyongbangkamgil-Tang and the extracted powder of Pilyongbangkamgil-Tang were tested for analgesic effects; Kamipilyongbangkamgil-Tang has the most effective analgesic function, then Pilyongbangkamgil-Tang, then the extracted powder, and I found that the t-test of those above prescriptions, in this order, should come to the result of voluntariness P〈0.001, P〈0.02, and P〈0.05 respectively. 2. Analgesic effects of pilyongbangkamgil-Tang and the extracted powder against the mouse pain induced by acetic acid have been strengthened by increase of double dosage and 4 fold dosage. 3. With Pilyongbangkamgil-Tang and Kamipilyongbangkamgil-Tang treated for rat, the increasing and the inhibitory rate of rat paw edema formation showed significantly statistical values, and the anti-inflammatory effect of the extracted powder of Pilyongbangkamgil-Tang is not supported so long than Pilyongbangkamgil-Tang. 4. The anti-inflammatory effect of Kamipilyongkamgil-Tang on the edema formed at the rat paw appeared significantly statistical value than the other sample.
朴恒基,蔡炳允 慶熙大學校 1984 論文集 Vol.13 No.-
In order to evaluate clinical efficacy and to improve the recipe of Sinhyotaglisan which has widely been used in "Ong-Jeaw" disease since the Sung Dynasty, a series of tests was conducted in vivo and vitro by resolving extracted powder and compound of each of the following samples: extracted powder of Sinhyotaglisan (Sample Ⅰ), Gamisinhyotaglisan A (Sample Ⅱ) obtained by adding to Sinhyotaglisan Angelicae Daliuricae Radix, Glieditisae spina, Manitis Squama and Trichosanthes Radix, and Gamisinhyotaglisan B (Sample Ⅲ) obtained by adding to Sinhyotaglisan Olibanum, Myrrha, and Fritillariae thunbergii Bulbus; and compounds of each extracted powder with the above three prescriptions(Samples Ⅳ, Ⅴ, and Ⅵ). The experimental results from the above prescriptions showed the analgesic, anti-inflammatory, anti-pyretic and antibiotic-like effects or activities as follows: 1. In analgesic activity, every experimental group proved a statistical significance in the contrast with control group. 2. In anti-inflammatory activity, extracted powder of Gamisinhyotaglisan A, the compound of each extracted powder and extracted powder of Gamisinhyotaglisan B (Samples Ⅱ, Ⅴ and Ⅲ) showed a significant difference as compared with the control group. 3. In anti-pyretic effect, extracted powder of Gamisinhyotaglisan A, the compound of each extracted powder and extracted powder of Gamisinhyotaglisan B (Samples Ⅱ, Ⅴ and Ⅲ) were effective in lowering the normal body temperature. 4. Antibiotic-like activities of the above prescriptions in liquid media were as follows: 1) Samples Ⅰ, Ⅱ, Ⅳ and Ⅴ for Staphylococcus aureus ATCC 25923 showed less than 25mg and Samples Ⅲ and Ⅵ showed less than 50mg of Minimal Inhibitory Concentration (MIC) in tube-dilution tests, also. 2) MICs of Samples Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ and Sample Ⅵ for Streptococcus pyogenes Rosen-bach 1884 recorded 25mg/ml and 50mg/ml, respectively. 3) MICs of Sample Ⅰ, Sample Ⅱ, Samples Ⅲ, Ⅳ, Ⅴ and Sample Ⅵ for Pseudomonas aeruginosa ATCC 10490 showed 20mg/ml, 25mg/ml, 50mg/ml and 100mg/ml, respectively. 5. Inhibitory effects of these prescriptions with 25mg contained per Disc on blood agar plate were as follows: The diameters of inhibition zone of (1) Staphylococcus aureus ATCC 25923 to Sample Ⅴ and (2) Streptococcus pyogenes Rosenbach 1883 to Samples, Ⅰ, Ⅱ, Ⅳ, and Ⅴ, and (3) Pseudomonas aeruginosa ATCC 10490 to Sample I were larger than other Samples, respectively. 6. The analgesic, anti-inflammatory and anti-pyretic activities were most remarkable in extracted powder of Gamisinhyotaglisan A and anti-bacterial activity was most remarkable in extracted powder of Sinhyotaglisan, while all of clinical effects on these activities were experimentally recognized in extracted powder and compund of each extracted powder of Gamisinhyotaglisan A.