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스테비아 잎과 마디 배양에 의한 부정신초 형성 및 식물체 재분화
박학봉 ( Hak Bong Park ),최정란 ( Jeong Ran Choi ),고정애 ( Jeong Ae Ko ) 전북대학교 농업과학기술연구소 2009 농업생명과학연구 Vol.40 No.2
고빈도 스테비아 식물체를 재분화 시키기 위해 잎과 마디를 2,4-D, NAA, IBA와 BA를 혼용처리한 MS 배지에 배양하였다. 절편체 종류 및 식물생장조절물질의 종류와 농도가 캘러스 유도, 신초분화 및 식물체 재분화에 미치는 효과를 조사하였다. 잎 절편체에서는 캘러스형성이, 마디 절편체에서는 액아의 분화 및 신장이 양호하였으며 잎절편 배양결과 0.5mg/L 2,4-D와 0.5mg/L BA 혼용처리시 3.5%, 2.0mg/L NAA와 2.0mg/LBA 혼용처리시 24%, 3.0mg/L IBA와 3.0mg/LBA 혼용처리구에서는 36.2%신초가 분화되어 가장 효과적이었다. 마디배양의 경우 마디의 위치 및 치상방향에 따라 캘러스형성, 액아 분화 및 신장에 영향을 미쳤으며 캘러스 형성에는 정단부위쪽 마디가, 그리고 액아의 분화 및 신장은 정단부위 보다는 중앙 및 하단 부위가 양호하였다. 마디의 수직 치상이 수평치상에 비해 액아 분화 및 신장에 효과적이었고, 잎 절편배양에서와 같이 2,4-D와 BA, NAA와 BA 혼용처리보다는 IBA와 BA 혼용처리가 캘러스형성이 양호하였으며, 특히 3.0mg/L IBA와 3.0mg/L BA 혼용처리에서 배양 개체 당 6개의 식물체의 재분화 되어 가장 효과적이었다. In order to produce the high frequency regenerated plants, leaves and stem node segments of Stevia rebaudiana Bertoni M. were cultured on MS medium containing 2,4-D, NAA, IBA and BA. We investigated the effects of kinds of explants, kinds and concentration of plant growth regulators on callus induction, shoot differentiation and plant regeneration. Callus induced easily in the leaf segment and axillary bud or shoot are ready to differentiate or elongate in the stem node segment. In the leaf segment culture, percentages of shoot differentiation were 3.5%, 24% and 36.2% through on MS medium with 0.5 mg/L 2,4-D plus 0.5mg/L BA, 2.0mg/L NAA plus 2.0mg/L BA and 3.0mng/L IBA plus 3.0mg/L BA, respectively. The part of stem node and direction of inoculation affected significantly on callus induction, axillary bud differentiation and shoot elongation in the culture of stem node. The upper part of stem node with apical meristem is effective on the callus formation and the bottom part of that is apt to differentiate the axillary bud or elongate the shoot. Effective differentiated axillary bud could be recognized on the vertical inoculation than horizontal inoculation of stem node. The combination of IBA plus BA is the best plant growth regulators among the that of 2,4-D plus BA and NAA plus BA as like leaf segment culture. Eespecially, six plants per explant regenerated on the MS medium with 3.0mg/L IBA plus 3.0mg/L BA.
이신,강대우,박학봉,Lee, Sin,Kang, Dae-Woo,Park, Hak-Bong 대한화약발파공학회 2001 화약발파 Vol.19 No.1
Korean peninsula has the most mountainous areas such as mountains and hilly country, and it is surrounded by the sea on all sides but one. In this respect, a large scaled construction works have frequently been conducted. However, it is not easy to porform a large scale blasting work without giving any harm to houses or facilities nationwide. Therefore, blasting work becomes more closely related to maintenance thing due to the development of the downtown or a large structure for key facilities. Many researches on blast in the open space and tunnel blasting have been conducted. On the contrary, research on underwater blasting operations is comparatively scanty even though much more necessity of marine development is required. In this respect, this study aims to investigate the characteristics of underwater blasting operations and to make a comparative study with blast in the open space. As a result of examining into the characteristics during underwater blasting operations, the around oscillation in case of underwater blasting operations shows significantly low compared to that in case of blast in the open space, and this means that much more cautious altitude must be taken in designing underwater blasting operations compared to the design of blast In the open space. As a result of analysis on the difference between a square root and a cube root In the equation of estimating oscillations in the actual site, it is shown that it is shown to apply a square root for the estimation of oscillation at 60 meters in case of underwater blasting operations and at 22 meters case of general blast in the open space.