사람 Neuroblastoma SH-SY5Y 세포주에서 Opiate 내성에 의한 c-myc 유전자 표현

박창교,권지윤,서성일,김수경,Park, Chang-Kyo,Kwon, Gee-Youn,Suh, Sung-Il,Kim, Soo-Kyung 대한약리학회 1997 The Korean Journal of Physiology & Pharmacology Vol.1 No.6

The mechanisms underlying opiate tolerance and dependence are not fully understood. We used human neuroblastoma SH-SY5Y cells as a model system for studying effects of morphine tolerance and withdrawal on c-myc induction and cAMP levels. It has been reported that regulation of c-fos by acute and chronic morphine withdrawal is mediated through alterations in CREB transcription factor. In this study, we examined the effects of morphine tolerance on c-myc expression and cAMP concentrations. The activation of opiate receptors by an acute morphine administration resulted in an increase in c-myc mRNA and a decrease in cAMP concentrations in a dose-dependent manner $(5,\;10,\;15,\;and\;20\;{\mu}M)$. On the other hand, the chronic treatment of morphine $(10\;{\mu}M\;for\;six\;days)$ did not induce the elevated expression of c-myc mRNA. The c-myc expression was slightly inhibited in comparison with that of the acute morphine response. However, cAMP concentrations were increased with regard to morphine withdrawal response. These results suggest that the alterations in c-myc expression might imply a significant opiate regulation relating to morphine tolerance. This observation differs from increased expression of c-fos via regulation of cAMP pathway.

심근 허혈-재관류 손상에 미치는 β 아드레날린성 수용체 봉쇄약물의 영향

김수경,이성룡,박창교 啓明大學校 醫科大學 1995 계명의대학술지 Vol.14 No.3

The effects of β adrenoceptor blockers(metoprolol, acebutolol, and atenolol) on the biochemical changes occurring following ischemia(60min) and reperfusion(20min) were examined in the isolated Langendorff perfused rat heart after chronic adriamycin(1㎎ /㎏ /day, i. p. for 4wks) treatment. The purpose of this study was to investigate whether the protections of βadrenergic blockers would be involved in ischemia-reperfusion injury of chronic adriamycin treated rat heart. The βadrenoceptor blockers showed the decrease of lactate dehydrogenase(LDH) activity and malondialdehyde(MDA) content and the no significance of superoxide dismutase(SOD) activity. Metoprolol and atenolol exhibited the increase of LDH activity in dose dependent. Our results indicate that the significant protection is not observed by increasing the doses of metoprolol. acebutolol, and atenolol and appear to be independence on the doses of β adrenoceptor blockers in chronic free radical injured rat heart. Acebutolol which has the membrane stabilizing activity shows relative protective effect on the cardiac damage comparing with metoprolol and atenolol.

우리나라 회계교육(會計敎育)의 개선(改善)에 관(關) 한 연구(硏究)

박창교(朴彰敎) 한국경영교육학회 1987 경영교육연구 Vol.1 No.-

간질 발작시에 나타나는 조기유전자 발현의 변화

박창교,김수경 啓明大學校 醫科大學 1996 계명의대학술지 Vol.15 No.3

Cellular immediate early gene expressions have been demonstrated to be modulated in response to a variety of seizure-inducing stimuli. In this study, two immediate early genes, c-fos and c-myc were investigated in response to the convulsants such as kainic acid(KA, 10mg/kg, ip). pentylenetetrazole(PTZ, 40mg/kg, ip), and N-methy1-D-aspartate(NMEA, 60mg/kg, ip) in three regions of rat brain : cerebral cortex, striatum, and brain stem. The KA and PTZ treatment slightly increased the transcription of c-fos in cerebral cortex, striatum, and brain stem. The MK-801 or baclofen pretreatment did not block the elevated expression of c-fos by KA and PTZ treatment. The NMDA treatment showed a reversal response of KA and PTZ treatment. The c-myc expression did not show a significant change in each of convulsant treatment groups and MK-801 or baclofen pretreatment did not exhibit considerable differenes. Therefore, c-fos could be one of the first seizure-related genes expressed in a cascade of events underlying different post-translational products, which result in diverse functional consequences depending on the brain sites.

메스암페타민 의존 환자에서 바이오피드백 훈련의 효과 : 정량화 뇌파를 이용한 예비연구

박창교(Changkyo Park),권도훈(Dohoon Kwon),조성남(Sungnam Cho),김양태(Yangtae Kim),김기성(Kiseong Kim),조수현(Soohyun Joe) 한국중독정신의학회 2012 중독정신의학 Vol.16 No.2

Objectives : In this study, authors investigated the neurophysiological effect of biofeedback training in patients with meth-amphetamine dependence using quantitative EEG (qEEG). Methods : Methamphetamine-dependent patients willing to receive biofeedback therapy were recruited in an addiction ward. qEEG measurements were conducted before and after each training session. Theta (4-8 Hz) relative power was measured and com-pared before and after biofeedback qEEG of each session. Results : A total of 49 pairs of relative power before and after bio-feedback qEEGs were obtained from 11 subjects with methamphetamine dependence. The right temporal theta relative power showed a significant decrease after biofeedback training. The right frontal theta relative power in the early biofeedback train-ing sessions showed a significant decrease compared with the later training sessions. Additionally, the right temporal theta relative power after the last-biofeedback training session also showed a significant decrease compared with the biofeedback before the first training session. Conclusion : These results suggested that the application of biofeedback training program changed brain waves and thus, could be a useful method of treat-ment for methamphetamine dependence.

방사선조사에 의해 발생되는 세포고사에 대한 Cysteamine의 효과

최영민,박창교,조흥래,이형식,허원주,Choi, Young-Min,Park, Chang-Gyo,Cho, Heung-Lae,Lee, Hyung-Sik,Hur, Won-Joo 대한방사선종양학회 2000 Radiation Oncology Journal Vol.18 No.3

목적 : 방사선에 의한 세포고사의 경로와 방사선보호제의 일종인 cysteamine (${\beta}$-mercaptoethylamine)이 방사선에 의한 세포고사에 미치는 영향을 알아보고자 하였다. 대상 및 방법 : HL-60 세포주를 대상으로 대조군, 방사선조사군, cysteamine 전처치군(1 mM, 10 mM) 으로 나누어서 실험을 하였다. 방사선은 6 MV로 10 Gy 일회 조사하였고, cysteamine은 방사선조사 1시간 전에 처치하였다. 세포고사의 경로를 알아보기 위하여 대조군과 방사선조사군에서 Caspase딕의 활성도를 측정하였고, 세포고사에 대한 cysteamine의 영향을 알아보기 위하여 방사선조사 후 1.5, 3, 6, 24시간에서 각 실험군의 생존 세포 수, caspase-3 의 발현과 활성도, poly (ADP-rlbose) polymerase (PAHP)의 발현 등을 측정하여 비교하였다. 결과 : 세포사망수용체에 의한 세포고사의 발생과 관련이 있는 Caspase겨의 귿성도는 방사선조사에 영향을 받지 않았다(p>0.05). 생존 세포 수는 방사선조사 6시간 후부터 감소되었는데(p>0.05), 1 mM cysteamine 전처치군에서는 감소되지 않고 대조군과 비슷하게 유지되었다. 세포고사의 실행 단계라고 알려진 Caspase-3의 발현은 각 실험군들 사이에 차이가 없었으나, 활성도는 방사선조사 후에 증가되었고(P>0.05) 1 mM cysteamlne 전처치에 의해 증가가 감소되는 경향이었다. Caspase-3의 활성에 의해 발생되는 PARP 분해산물(24 kD)의 발현이 방사선조사 후에 관찰 되었는데, 1 mM cysteamine 전처치군에서는 발현의 감소가 관찰되었다. 결론 : 방사선에 의한 세포고사는 세포사망수용체에 의한 세포고사와는 다른 경로를 거치고, 1 mM cysteamine 전 처치는 방사선조사에 의한 세포고사의 발생을 억제하는 경향이 있는 것으로 생각된다. Purpose : To Investigate the pathways of radiation induced apoptosls and the effect of cysteamine (${\beta}$-mercaptoethyiamine), as a radioprotector, on it. Materials and Methods : HL-50 ceils were assigned to control, irradiated, and cysteamlne (1 mM, 10mM) pretreated groups. Irradiation was given In a single fraction of 10 Gy (6 MV x-ray) and cysteamine was administered 1 hour before irradiation. The activities of caspase-8 were measured in control and irradiated group to evaluate its relation to the radiation Induced apoptosis. To evaluate the role of cysteamine In radiation Induced apoptosis, the number of viable cells, the expression and activity of caspase-3, and the expression of poly (ADP-ribose) polymerase (PARP) were measured and compared after irradiating the HL-60 celis with cysteamine pretreatment or not. Results : The intraceliular caspase-8 activity, known to be related to the death receptor induced apoptosis, was not affected by irradiation(p>0.05). The number of viable cells began to decrease from 6 hours after irradiation (p>0.05), but the number of viable cells In 1 mM cysteamine pretreated group was not decreased after irradiation and was similar to those in the control group. In caspase-3 analyses, known as apoptosis executioner, its expression was not different but its activity was Increased by irradiation(p>0.05). However, this Increase of activity was suppressed by the pretreatment of 1 mM cysteamine. The cleavage of PARP, thought to be resulted from caspase-3 activation, occurred after irradiation which was attenuated by the pretreatment of 1 mM cysteamine. Conclusion : These results show that radiation induced apoptotic process is somewhat different from death receptor induced one and the pretreatment of 1 mM cysteamine has a tendency to decrease the radiation-induced apoptosis in HL-60 cells.

방사선 조사에 의해 발생하는 세포고사에 대한 Cysteamine의 효과

최영민,박창교,유병길,김태희,김만 국군중앙의무시험소 1999 국군중앙의무시험소논문집 Vol.14 No.1999

Soman에 의한 경련발작시 Zinc 착화제의 효과에 관한 연구

오세옥,박도윤,박창교,김영자,하미현,김만,강태석,김태희 국군중앙의무시험소 1999 국군중앙의무시험소논문집 Vol.14 No.1999

Effects of Neuroleptics on the Opioid Receptor Binding in the Mouse Striatum

김수경,이성룡,박창교,Kim, Soo-Kyung,Lee, Seong-Ryong,Park, Chang-Gyo The Korean Society of Pharmacology 1994 대한약리학잡지 Vol.30 No.3

Our purpose was to gain insight into a possible modulatory role for ${\mu},\;{\delta},\;and\;{\kappa}$ opioid receptors by neuroleptics (chlorpromazine, thioridazine, haloperidol, sulpiride, and pimozide) in chronic morphine 5 mg/kg and 20 mg/kg treated mouse striatum. We attempted quantitative receptor assays using highly specific radioligands, $[^3H]\;DAGO\;([D-Ala^2,\;N-Mephe^4,\;Glycol^5]\;enkephalin)$, $[^3H]DPDPE\;([D-Pen^2,\;D-Pen^5]\;enkephalin)$ and $[^3H]\;DPN(diprenorphine)$ to measure the binding affinity in the experimental groups. The decrease of $[^3H]DAGO$ binding was potentiated by sulpiride and pimozide in the chronic morphine treatment (5 mg/kg and 20 mg/kg). The decrease of $[^3H]DPDPE$ binding was inhibited by chlorpromazine, thioridazine, haloperidol, sulpiride, and pimozide in chronic morphine treatment (5 mg/kg and 20 mg/kg). The decrease of $[^3H]\;DPN$ binding was significantly inhibited by chlorpromazine, thioridazine, sulpiride, and pimozide in chronic morphine 20 mg/kg treatment. $[^3H]\;DPN$ binding on the neuroleptics was antagonized by naloxone pretreatment in chronic morphine 20 mg/kg treatment. These findings suggest that neuroleptics influence opposing tonically active on the ${\delta},\;and\;{\kappa}$ opioid receptor compared with ${\mu}$ opioid receptor in the chronic morphine treated mouse striatum. 이 연구에서는 선조체에서 opioid 신경계와 dopamine 신경계의 상호 관계를 알아보기 위해서 morphine을 5m/kg, 20 mg/kg로 10일간 복강내 투여한 후 chlorpromazine, thioridazine, haloperidol, sulpiride, pimozide를 투여하였다. Opioid ${\mu},\;{\delta},\;{\kappa}$ 수용체의 binding의 변화를 관찰하고자 $[^3H]\;DAGO$, $[^3H]\;DPDPE$, 및 $[^3H]\;DPN$ binding assay를 하였으며, 그 결과 morphine (20 mg/kg) 장기 투여된 실험군에서 $[^3H]\;DAGO$, $[^3H]\;DPDPE$, 및 $[^3H]\;DPN$ 결합이 감소되었다. Morphine 20 mg/kg 장기 투여군에 chlorpromazine, thioridazine 주사시에는 morphine 5mg/kg 투여군에 비하여 $[^3H]\;DAGO$ 결합의 감소와, $[^3H]\;DPDPE$, 및 $[^3H]\;DPN$ 결합의 증가를 나타내었고, haloperidol 주사군은 $[^3H]\;DAGO$, $[^3H]\;DPN$ 결합의 감소, 및 $[^3H]\;DPDPE$ 결합의 증가를 나타내었다. Sulpiride, pimozide 주사군은 morphine 5 m/kg 투여군에 비하여 20m/kg 투여군에서 $[^3H]\;DAGO$, $[^3H]\;DPDPE$, 및 $[^3H]\;DPN$ 결합의 증가를 나타내었다. 이상의 결과로 보아 각 약물간의 opioid 결합에 대한 차이점은 있었으나, morphine 5mg/kg 투여군보다 20m/kg 투여군에서 $[^3H]\;DPDPE$ 및 $[^3H]\;DPN$의 결합이 증가의 경향을 보임으로써, 다량의 morphine을 투여했을 때 ${\mu}\;opioid$ 수용체에 비하여 ${\delta}와 ${\kappa}\;opioid$ 수용체가 더 활성화되는 것을 알 수 있었다.

RCP induces FAK phosphorylation and ovarian cancer cell invasion with inhibition by curcumin

최소라,김유나,박창교,조경화,조도연,이회영 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Rab coupling protein (RCP) aggravates cancer cell metastasis and has been implicated in various cancer patient outcomes. Recently, we showed that RCP induces Slug expression and cancer cell invasion by stabilizing the β1 integrin protein. In the present study, we demonstrated that FAK is implicated in RCP-induced EGFR phosphorylation and ovarian cancer cell invasion with inhibition by curcumin. Ectopic expression of RCP induced FAK phosphorylation, which links β1 integrin with EGFR and participates in a positive regulation loop with EGFR. Interestingly, we observed for the first time that curcumin attenuates RCP-induced ovarian cancer cell invasion by blocking stabilization of β1 integrin and consequently inhibiting FAK and EGFR activation, providing potential biomarkers for ovarian cancer and therapeutic approaches for this deadly disease.