HPLC를 이용한 농식품 중 당류의 신속정량 분석

원선이 ( Seon Yi Won ),서재순 ( Jae Soon Seo ),강희윤 ( Heui Yun Kang ),이용선 ( Yong Seon Lee ),최용민 ( Young-min Choi ),이해길 ( Hae Kil Lee ),박인태 ( In Tae Park ) 한국산업식품공학회 2016 산업 식품공학 Vol.20 No.4

HPLC를 이용하여 농식품에 함유된 fructose, glucose, sucrose, maltose, lactose 등 5종의 당류에 대한 신속하고 정확한 동시분석법을 확립하기 위하여 시험을 실시하였다. 수용성 당류의 HPLC 분석조건은 RI 검출기를 이용하여 column은 ZORBAX Carbohydrate (4.6 ㎜ ID×250 ㎜, 5㎛)를 이동상은 75% ACN을 사용하였고 칼럼온도 35℃, 시료 주입량은 10 μL, 유속은 1 mL/min의 조건으로 30분간 분석하여 방해 peak없이 5종의 표준용액을 분리하였다. 표준용액 검량선의 R²=0.999 이상으로 5종 모두 직선성이 우수하였고, 표준용액의 크로마토그램을 이용하여 분석의 정량한계와 검출한계를 산출하였다. 농식품의 당류 추출방법을 구명하기 위하여 표준인증물질 BCR644을 이용 추출 방법별 정확도를 비교한 결과, 증류수 100% 추출처리에서 fructose 95.7%, sucrose 98.7%, lactose 102.7%로 가장 우수하여 농산물 당류 추출시 적용하였다. 내부표준물질인 분유를 이용하여 반복성과 재현성을 확인하여 본 분석법에 대한 정밀도를 검증하였고 reference value 값으로 glucose는 1.17 g/100 g, maltose는 0.85 g/100 g, lactose는 45.54 g/100 g으로 설정하였고, 허용한계 95% 수준(2*SD)을 경고한계로, 허용한계 99.7% 수준(3*SD)을 거절한계로 산출하여 품질관리차트를 작성하고 농식품 당류 분석에 활용하였다. To determine sugars content of agricultural products and foods, simultaneous quantitative analysis was carried out on fructose, glucose, sucrose, maltose, and lactose by HPLC-RI. Analysis conditions were set as column ZORBAX car-bohydrate (4.6 ㎜ ID×250 ㎜, 5 ㎛), the mobile phase of 75% ACN, the column temperature of 35℃, sample injection amount of 10 μL and the flow rate of 1 mL/min. Five standard solutions were isolated without interfering peak within 30 minutes and the calibration curves of standard were confirmed excellent linearity from 0.10% to 1.00% with R²≥0.999. Based on the chromatogram of the standard solution, the limit of quantification (LOQ) and the limit of detection (LOD) values were calculated. The accuracy of the analytical values were highest at 100% water extraction method to the fructose 95.7%, sucrose 98.7%, lactose 102.7% respectively, compared with reference value of a certified reference material (BCR644), by applying the four solvent extraction methods. Using an in-house quality control material (infant formula), repeatability and reproducibility values of this experiment were verified on the basis of AOAC guideline and reference values were set up at 1.17 g/100g of glucose, 0.85 g/100g of maltose, and 45.54 g/100g of lactose. Quality control charts were drawn up and used for sugars analysis of agricultural products.

태권도 몸통 돌려차기 동작에 대한 운동역학적 분석

지용석(Yong Seok Ji),박인태(In Tae Park),강영석(Young Seok Kang) 한국사회체육학회 2008 한국사회체육학회지 Vol.0 No.34

This Study has conducted an analysis on the Biomechanical Variables about Body-Dolyeochagi Motion of Taekwondo with the object of 5 superior players and the other 5 inferior players, among 10 persons of the lightweight-class college players, then obtained the conclusion as followings. The necessary time when carrying out Body-Dolyeochagi of Taekwondo was appeared as desirable to inflict a great impact to the target object within shorter time by increasing its pre-speed by means of making the articular angle of the knee to be bent quickly at the moment, and in case of the body pivot, at the moment when the right foot is removing from the ground, the inside-turning quickly of the right hip joint using the repulsive power of ground makes the situation of body pivot less, and it lets the knee joint bent rapidly where the moment arm is diminished, thence it was appeared as desirable to transmit the impulse which enables to attack the target object quickly. In order to transmit a great power to the target object by the hip joint angle, erect the body at the initial stage, keeping Long Axis Rotation widely, and it is also desirable to metastasize the momentum to the target object by means of winding the hip joint angle, and in case of the knee joint angle, the momental knee joint to be wound in maximum, by making the knee joint stretched greatly utilizing the Law of Action and Reaction at E3 time-point, it showed up as desirable to blow a great impact to the target object. Likewise, in case of the angle of foot articulation, when bending of the knee joint is maximized due to rapid extension of the foot articulation, it is desirable to beat accurately to the target object when impacting, and in the instance of External Oblique Muscle of Abdomen at Average Value of Integral Electromyogram for the principal muscle, owing to the rapid turning of the vertebrae at the moment of impact, its Antagonist was acted more, and in case of Erector Spinae Muscle, it is known that its body-trunk was quickly turned in the next motion of the superior players group. In case of Rectus Femoris and Biceps Femoris, the Bending and Extending Motion of the hip joint have been made more efficiently in the superior players group, and in Medial Gastrocnemius Muscle, the Bending and Extension of the knee joint were not efficient, and in case of Tibialis Anterior Muscle, since Plantar Flexion of the ankle joint was effectively established in the superior players group, it was appeared that an efficient impact thereat has been accomplished.

태권도 수련생의 수련특성이 도장만족도에 미치는 영향

이종근(Jong Kun Lee),박인태(In Tae Park),지용석(Yong Seok Ji) 한국체육교육학회 2010 한국체육교육학회지 Vol.15 No.2

본 연구는 태권도 수련생의 수련특성이 도장만족도에 미치는 영향을 규명하고, 태권도 수련생들의 수련특성을 파악하여 도장운영에 필요한 경영전략을 세우는데 필요한 기초자료를 제공하는데 그 목적이 있다. 연구대상은 2010년 현재 청주시에 위치한 대권도장을 이용하는 수련생 150명을 모집단으로 선정한 후 설문지를 배포하였고, 이 중 회수된 설문지는 141부였다. 분석결과 첫째, 성별 요인에서는 수련목적만이 유의한 차이가 있는 것으로 나타났고, 연령 요인에서는 수련시간, 수련기간, 수련목적 모두 유의한 차이가 있는 것으로 나타났다. 그리고 생활수준 요인에는 수련기간, 수련목적이 유의한 차이가 있는 것으로 나타났다. 둘째, 수련특성 하위요인 중 수련시간 요인에서는 도장만족도 하위요인 프로그램만족, 시설만족, 직원만족, 사회성만족 모두 긍정적인 영향을 미치지 않는 것으로 나타났고, 수련특성 하위요인 중 수련기간 요인에서는 도장만족도 하위요인 프로그램만족, 직원만족 요인이 유의한 영향을 미치는 것으로 나타났다. 그리고 수련특성 하위요인 중 수련목적 요인에서는 도장만족도 하위요인 프로그램만족 요인만이 유의한 영향을 미치는 것으로 나타났다. The goal of this study is looking for an effect how training characteristic of Taekwondo trainer have an influence on gymnasium satisfaction. The subject of study are trainers who are using the Taekwondo gymnasium located in Cheongju now in 2010 as population. Although we collected 150 by bias sampling of ramdom sampling, the last re-collected data was 148 and it was 98% higher return rate. Except for 7 among unreliable datum, 141 datum was used in the last analysis. It was 95.2% valid analysis rate. The methods of statistic analysis for dealing datum were verified by using frequency analysis, factorial analysis, Cronbach`s a test, one-way ANOVA, multiple regression analysis, and so on. In this way we got a result as follows. First, to verify differences between demographic characteristic and training characteristic, we conduct one-way ANOVA setting Taekwondo training characteristic as independent variable and vital statistical trait as dependent variable. As a result, we found that in sexual factor goal for training only has significant differences as p<.05 in significance level. In age factor, all of training time, training period and goal for training has significant differences as p<.001 in significance level. In living standard factor, training period as p<.01 and goal for training as p<.05. Second, to verify an effect how training characteristic of Taekwondo trainer have an influence on gymnasium satisfaction, we conducted multiple regression analysis. In training time factor among training characteristic subordinated factor, all of gymnasium, staff contentment and sociality contentment didn`t have a positive influence. In training period factor, program contentment factor have an influence on p<.001 and staff contentment do so p<.05 in significance level. And in goal for training factor, only program contentment factor do so p<.001 in significance level.

누룩 사용 발효주로 제조한 증류주의 숙성 용기 및 기간에 따른 특성

이대형(Dae-Hyoung Lee),박인태(In-Tae Park),이용선(Yong-Seon Lee),서재순(Jae-Soon Seo),정재운(Jae-Woon Jung),김태완(Tae-Wan Kim),김재호(Jae-Ho Kim),안병학(Byung-Hak Ahn) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.10

본 연구에서는 누룩 사용 발효주로 제조한 증류주를 국내에서 일반적으로 사용하고 있는 항아리와 스테인리스 용기에 숙성시키며 숙성시간별 증류주의 특성을 검토하였다. 알코올 분석 결과 알코올은 40.4∼39.3%로 시작하였으며 숙성기간이 증가함에 따라 항아리와 스테인리스 용기의 알코올은 조금씩 감소하는 경향을 나타냈다. 휘발 산도는 감압증류 항아리 및 스테인리스 용기 숙성에서 약간 상승하였다. Acetic acid의 함량은 숙성기간이 증가함에 따라 항아리와 스테인리스 용기 모두에서 변화하지 않았다. 2-Thiobarbituric acid(TBA)는 증류 직후 감압증류가 상압증류보다 낮았으며 숙성기간이 증가함에 따라 TBA 값은 항아리와 스테인리스 용기 모두 큰 변화가 없었다. 숙성에 따른 증류주의 fusel alcohol 중 n-propanol은 숙성기간이 증가함에 따라 상승하지 않았으며 iso-butanol은 항아리 숙성의 경우 측정값이 감소하였으나 스테인리스 용기는 변화가 없었다. 향기성분의 경우 항아리 보관에서 증류 직후보다 180일에서 좀 더 다양한 향기 성분이 측정되었으며 특히 고비점의 향기성분들이 많아졌다. 스테인리스 용기 역시 0일차보다 180일에 좀 더 다양한 향기 성분이 나타났으나 그 양은 상대적으로 항아리보다 적었다. 항아리 숙성 관능 결과 180일에는 감압 50 cmHg의 관능점수가 가장 높았으며 상압 80℃ 증류주는 0일에 비해 180일의 관능이 가장 많이 향상되었다. This study aimed to determine the quality characteristics of fermented wine using Nuruk according to the type of aging container (jar or stainless container) and aging time. Alcohol analysis was initiated at 40.4~39.3%, and alcohol contents in the jar and stainless container decreased as aging time increased. Volatile acid content slightly increased in the reduced pressure (RP) jar and stainless container and showed a different result in the atmospheric pressure (AP) container. Amount of acetic acid did not change either in the jar or stainless container as aging time increased. In the case of 2-thiobarbituric acid (TBA), RP was lower than AP after distillation, and TBA value did not change much either in the jar or stainless container. In the case of fusel alcohol by aging time, n-propanol content did not increase either in the jar or stainless container as aging time increased. Iso-butanol content was reduced in the jar but did not change in the stainless container. In the AP container, more aromatic components were measured on the 180th day of aging time than after distillation. High boiling point aromatic components increased in particular. In the stainless container, more aromatic components were generated on the 180th day of aging, but their amount was relatively lower than in the jar. In the jar aging sensory test, the sensory score at RP 50 cmHg was highest on the 180th day of aging. In the case of AP 80°C liquor, sensory score on the 180th day was the most improved.

실내 위치에 따른 혼합 식재 선인장과 다육식물의 생장

송천영(Cheon Young Song),이상덕(Sang Deok Lee),박인태(In Tae Park),이정식(Jeong Sik Lee) 한국원예학회 2007 원예과학기술지 Vol.25 No.4

혼합식재한 선인장 및 다육식물을 재배온실, 창가, 실내내부와 외부에서 4개월간 관리한 후에 고사율 및 생육조사를 하였다. 식물의 생육은 실내보다 재배 온실 및 창가에서 양호하였다. 백운각, 용신목 및 비취전은 광도가 낮은 실내내부(4.2μmolㆍm⁻²s⁻¹) 및 외부(13.4μmolㆍm⁻²s⁻¹)에서 3-6㎝ 도장되었다. 광도가 낮은 실내 안과 바깥쪽에서 금호, 백운각, 용신목, 무륜주 및 십이지권은 고사되지 않았으나, 비모란, 금황환, 노토캑터스 마그니휘커스, 아악무 및 비취전은 20-40%가 고사되었고 특히 비모란 분홍은 100%고사되었다. 따라서 혼합식재한 선인장 및 다육식물을 실내에 들여 놓을 때 햇볕이 가장 잘 드는 창가에 놓고 관리하는 것이 식물의 도장 및 고사율을 줄일 수 있었다. This experiment was conducted to investigate the effect of indoor position on survivals and growth of several cacti and succulents planted in a pot. Echinocactus grusonii, Marginatocereus marginatus, Myrtillocactus geometrizans, Pachycereus pringlei, Eriocactus leninghausii. Gymnocalycium mihanovichii var. friedrichii, Notocactus magnificus, Portulacaria afra v. foliis-variegatis, Haworthia fasciata, and Aloe squarrosa were positioned near to window, outside indoor, inside indoor and greenhouse for four months, and monitored their plant growth and mortality. The plants of indoor including outside and inside, very low light intensity ranged from 2.8 to 13.4 μmolㆍm⁻²s⁻¹, were overgrown in Marginatocereus marginatus, Myrtillocactus geometrizans, and Aloe squarrosa, and increased mortality in Gymnocalycium mihanovichii, Eriocactus leninghausii, Notocactus magnificus, Portulacaria afra v. foliis-variegatis, and Aloe squarrosa, but did not influence in the other plants. Plant height and width in the greenhouse and near to window tended to be higher than those of outside and inside indoor. Thus, proper indoor position of several cacti and succulents planted in a pot was near to window, showing the most strong light intensity in indoor.

밥맛이 우수한 경기지역 적응 조생종 벼 ‘정드림’

장정희(Jung-Hee Jang),최병렬(Byoung-Rourl Choi),원태진(Tae-Jin Won),이지혜(Ji-Hye Lee),이종형(Jong-Hyeong Lee),한상욱(Sang-Wook Han),장재은(Jae-Eun Jang),서재순(Jae-Soon Seo),지정현(Jung-Hyeun Ji),박인태(In-Tae Park),최해춘(Hae-Chun 한국육종학회 2021 한국육종학회지 Vol.53 No.2

‘Jungdream’ is an early maturing, high-quality rice cultivar developed in 2017 by the crop breeding team at Crop Research Division,GARES, Hwaseong, Korea. ‘Jungdream’ was first developed in 2008 from crossing a medium-late maturity cultivar ‘Chilbo’ and an early maturitycultivar ‘Ungwang’. The heading date of this cultivar is July 28thin the Gyeonggido Province. Its culm length is 63 cm, and it has 19 paniclesper hill and 82 spikelets per panicle. It is a medium-small grain brown rice cultivar with a 1000-grain weight of 20.6 g. Its cold toleranceis lower than that of ‘Hwaseong’, which is the standard cultivar in the central region, as is its degree of lodging tolerance. ‘Jungdream’ isresistant to rice blast and stripe virus diseases but is susceptible to bacterial leaf blight and other viral diseases and pests. The appearance ofmilled ‘Jungdream’ rice is clear, and its amylose content is 16.5%, which is lower than that of ‘Hwaseong’. The palatability of cooked ‘Jungdream’rice is better than that of ‘Chucheong’, which is the main cultivated crop in Gyeonggido. Its milling ratio is 75.0%, which is lower than thatof ‘Hwaseong’. The yield of milled ‘Jungdream’ rice was 5.42 MT/ha in the local adaptability tests during standard cultivation in four regionsfrom 2015 to 2017. ‘Jungdream’ is highly adaptable to the environmental conditions in the Gyeonggido Province (Registration No. 8236).

지역사회 복지차원의 생활체육 활성화를 위한 동호인조직 운영모형 연구

박인태(In Tae Park) 한국사회체육학회 2000 한국사회체육학회지 Vol.13 No.-

Korean sport for all club activities are under unsegmented level of professional club of sport for all, which is lacking self survival grounds. This prevented the club activities of sport for all from performing its own part and led the club to its standstill situation. Therefore, the purpose of this study is to analyze the whole procedure of sport club activities from its forming stage through settling stage and draw out the operating model of sport club to the situation of community. The implications of the study are as follows: 1. There needs to obtain self surviving ability by efficient management and reasonable supporting system of sport for all clubs. 2. There needs to build cooperating system with the regional groups or organization for supporting sport facilities and their efficient usage. 3. There needs to maximize the exercising effect of sport participants through keeping sport instructors and their efficient positioning, which will make the program operated smoothly. 4. There needs to induce people to take part in sport activities through making them join the sport clubs with interests and enthusiasm by efficient PR. 5. There needs to support new sport clubs and also needs to make administrative or financial support more efficiently.

접목선인장 소정 신품종 ‘순정’ 육성

박인태(In Tae Park),손기철(Ki Cheol Son),이상덕(Sang Deok Lee),조창휘(Chang Hui Cho),김순재(Soon Jae Kim) 한국원예학회 2003 한국원예학회 학술발표요지 Vol.2003 No.10

볏짚 시용에 따른 벼 재배 논에서의 메탄 배출계수 개발에 관한 연구

주옥정 ( Ok Jung Ju ),원태진 ( Tae Jin Won ),조광래 ( Kwang Rae Cho ),최병열 ( Byoung Rourl Choi ),서재순 ( Jae Sun Seo ),박인태 ( In Tae Park ),김건엽 ( Gun Yeob Kim ) 한국환경농학회 2013 한국환경농학회지 Vol.32 No.3

BACKGROUND: Accurate estimates of total direct CH4 emissions from croplands on a country scale are important for global budgets of anthropogenic sources of CH4 emissions and for the development of effective mitigation strategies. Methane production resulted by the anaerobic decomposition of organic compounds where CO2 acts as inorganic electron acceptor. This process could be affected by the addition of rice straw, water management and rice variety itself. METHODS AND RESULTS: Rice (Oryza sativa L. Japonica type, var Samkwangbyeo) was cultivated in four plots: (1) Nitrogen-Phosphorus-Potassium (NPK) (N-P2O5-K2O:90-45-57 kg/ha); (2) NPK plus 3 Mg/ha rice straw (RS3); (3) NPK plus 5 Mg/ha rice straw (RS5); (4) NPK plus 7 Mg/ha rice straw (RS7) for 3 years (2010-2012) and the rice straw incorporated in fall (Nov.) in Gyeonggi-do Hwaseong-si. Gas samples were collected using the closed static chamber which were installed in each treated plot of 152.9 m2. According to application of 3, 5, 7 Mg/ha of rice straw, methane emission increased by 46, 101, 190%, respectively, compared to that of the NPK plot. CONCLUSION(S): We obtained a quantitative relationship between CH4 emission and the amount of rice straw applied from rice fields which could be described by polynomial regression of order 2. The emission scaling factor estimated by the relationship were in the range of IPCC GPG (2000).

05 포스터 발표 : 토양환경 분야PS-04 ; 국내 주요 논토양 토성별 메탄발생 잠재력 평가에 관한 연구

주옥정 ( Ok Jung Ju ),조광래 ( Kwang Rae Cho ),김대균 ( Dae Kyun Kim ),김영순 ( Young Sun Kim ),박인태 ( In Tae Park ) 한국환경농학회 2014 한국환경농학회 학술대회집 Vol.2014 No.-

메탄은 담수환경 조건에서 비교적 많이 발생하며 이와 관련된 연구로는 유기물의 투입에 따른 메탄 생성, 기후조건 등 주변 환경조건에 따른 메탄생성, 벼의 품종별 및 물 관리에 따른 메탄 생성등이 있다. 그러나 대부분의 연구가 메탄 순방출량(net flux)에 집중되어 있으며, 토양생태학적인 메탄 발생의 기작구명 연구는 거의 진행되지 않고 있다. 따라서 본 연구에서는 메탄 발생 저감 기술 개발을 위한 기초자료로 활용코자, 국내 주요 논토양 8개 지역 27지점에 대해 무작위 샘플링법으로 토양시료를 채취하여 메탄생성균, 산화균, 발생량 등을 분석하였다. 토양시료채취는 2013년 4월, 10월,2014년 3월에 실시하였으며, core sampler 안에 acryl로 제작된 직경 4.9cm, 높이 5cm의 용기를 장착하여 채취한 후 밀봉하여 실험실로 운반하였다. 메탄발생량은 WFPS (Water- Filled Pore Space)100% 조건하에서 28℃ 항온.항습기에서 48~72시간 배양하여 측정하였으며, 토양 DNA추출은 토양0.3~0.5g을 취하여 Ultra Clean Soil DNA Isolation Kit (MoBio Laboratories, Solana Beach, CA,USA)을 이용하여 분석하였다. 메탄 생성균 및 산화균의 풍부도는 RT-qPCR방법을 이용해 측정하였으며, CFX96TM Real-time PCR Detection System(Bio-Rad, Hercules, CA, USA)과 SYBR Green (Bio-Rad, Hercules, CA, USA) 검출 용액이 사용되었다. 토성별 평균 메탄발생량 및 메탄산화균의 풍부도는 sand 함량이 가장 많고(77%) clay 함량이 적은(2% 미만) 양질사토(loamy sand)에서 가장 크게 나타났으며, 대체로 clay 함량이 많은 토양에서 메탄 발생량이 작은 경향이었다.