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진도견 정자형성계 세포들의 Lectin-binding patterns
박영석,이성호,Park, Young-seok,Lee, Seong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
The lectin-binding patterns in the testis of the sexually matured Jindo dog were investigated to study the distribution of glycoconjugates in the seminiferous tubule under light and transmission electron microscopy. Positive reactions to Wheat germ agglutinin(WGA) and Dolichos biflorus agglutinin (DBA) were observed in the Sertoli cell and in the residual body of spermatid with a stronger reaction in the Sertoli cell to the lectins than in the residual body. Strong reactions to Soybean agglutinin(SBA) and Peanut agglutinin(PNA) were observed in the acrosome vesicles of the Golgi- and cap-phase spermatid, while a moderate reaction was observed in the acrosome-phase, maturation-phase spermatid and the residual body. The acrosome area of the spermatid reacted intensively to Griffonia simplicifolia agglutinin( GS-I) when the cell was in the acrosome-phase and maturation-phase, and the same reaction to the GS-I was observed in the residual body. However, the seminiferous tubule did not react to Ulex europeus agglutinin I(UEA-I). The gold-labelling of the Sertoli cells with DBA resulted in positive reactions of the Sertoli cell column and processes when observed under the electron microscopy, while the Golgi-, cap- and acrosome-phase spermatids reacted positively to SBA in the peripheral low-dense area of the acrosome vesicle of spermatid. Based on these results, we concluded that differences in the lectin-binding pattern of the seminiferous tubules were recognized in the Jindo dog compared to other animals.
진도견 정세관의 정세포와 Sertoli 세포내 glycogen의 분포
박영석,이성호,Park, Young-seok,Lee, Seong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
In an effort to obtain basic data of carbohydrate metabolism during spermiogenesis of the sexually-matured Jindo dog, the glycogen distribution in the testis was investigated by light and transmission electron microscopy. Periodic acid thiocarbohydrazide silver proteinate physical development(PA-TCH-SP-PD) staining method provided better results in the detection of glycogen granules from Sertoli cells and germ cells than the periodic acid schiff(PAS) staining method did. Pre-treatment of the tissue sections with ${\alpha}$-amylase elicited a significant decrease in PA-TCH-SP-PD stained granules, which suggested that the stained granules were of glycogen origin. High concentration of the glycogen granules were observed in the Sertoli cells, especially in its column, sheet-like processes, club-like processes, and tubular processes. The glycogen granules were unevenly distributed in some Sertoli cell columns. These results strongly indicated that the Sertoli cells of Jindo dogs showed vigorous activity of carbohydrate metabolism.
박영석,이재홍,Park, Young-seok,Lee, Jae-hong 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.3
In order to study on the Sertoli cell, we attempt have been made to measure the average number of each germ cells per Sertoli cell on the 12 stages of cycle in matured korean Jindo dog. The fine structure of Sertoli cell junctional specialization was studied with electron microscope. The results were summarized as follows; 1. The average number of various germ cells associated with Sertoli cell was 9.77 to 13. 80 through stages of cycle and the total average number was 11.62. 2. Sertoli-Sertoli cell junctional specialization was present in seminiferous epilthelium, and Sertoli-spermatid cell junctional specialization rose from stage 8 spermatid, persisted to step 13 spermatid and then disappeared. The structure of Sedoli-spermatid cell juncticnal specialization was not similar to that of Sertoli cxlls. 3. Just after spermiation, free-surface of Sertoli-spermatid cell junctional specialization was replaced by Sertoli cell cytoplasm with tubulobulbar complex at the neiglaboring region observed. 4. The Sertoli cell process was located within the cytoplasm of late stage spermatids. Some membranes of residual body and spermatid cytoplasm partly disappeared, resulting in opening of the cytoplasm of spermatid into that Sertoli cell. This fact suggested that spermatid cytoplasm was partly eliminated.
진도견(珍島犬)의 정자형성(精子形成)과 Sertoli세포(細胞) 특수(特殊) 연접부(連接部)의 미세구조(微細構造) I. 진도견(珍島犬)의 정자형성(精子形成)에 관한 연구(硏究)
박영석,이재홍,Park, Young-seok,Lee, Jae-hong 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.3
Classification of the cycle of seminiferous epithelia into 12 stages by the morphological changes in acrosomal system and evaluation of the relative frequency of stages and the cell association were histologically performed in the mature Korean native Jin-do dogs. The results were summarized as follows; 1. The minimum number of type A spermatogonia averaged 1.01 at stages I, while maximum number averaged 2.47 at stages XII. Some type A spermatogonia divided at stage XII to produce the type intermediate(IN) spermatogonia at the following stage I. The type IN spermatogonia divided at stage IV to produce the type B spermatogonia at stage V. 2. The type B spermatogonia divided at stage VI to produce the preleptotene primary spermatocytes at stage VII. The secondary spermatocytes observed at stage XII. The secondary spermatocytes observed at stage XII divided to give rise to the round spermatids at the following stage I. The numbers of the first spermatocytes and spermatids were almost constant, respectively, through all the cycles of seminiferous epithelium. 3. The acrosomal vesicle was invaginated to occupy one third to half of spermatid nucleus at the cap phase, which was different from that of rodent and ruminant spermatid nuclei. 4. The relative frequencies of stages I to XII of seminiferous epithelia cycle were 10.34, 4.84, 5.03, 8.22, 10.86, 6.63, 6.42, 18.88, 10.17, 6.18, 7.62% and 4.81%, respectively.
형태 해부학적 특징을 바탕으로 한 자연치형 지르코니아 임플란트
박영석(Young-Seok Park),이승표(Seung-Pyo Lee) 대한치과의사협회 2014 대한치과의사협회지 Vol.52 No.9
The restorative treatment using titanium implants has become a standard procedures in everyday clinical practice. However, there are several concerns about the use of titanium as a materials. Above all, the grayish metal color provokes esthetic problems when the soft tissue around the implants is not optimal. Recently, zirconia has been emerging as an alternative to titanium due to its white color and superior physicochemical properties. In this paper, the current findings about zirconia implants was reviewed based on the results from animal and clinical experiments. In addition, the immediate implantation protocols using the natural tooth form zirconia implants was introduced as a novel procedures and future perspectives were discussed.
항공사 승무원의 서비스 회복 행동, 서비스 행동 및 서비스 품질에 미치는 문화적 영향에 관한 연구
박영석(Young Seok Park),변정우(Jung Woo Byun) 한국호텔외식관광경영학회 2011 호텔경영학연구 Vol.20 No.4
The purpose of this study is to find out the cultural influence on service recovery performance, service behavior and service quality of American and Japanese flight attendants working in an American air carrier. To accomplish this study, a total of 240 questionnaires were handed out to American and Japanese flight attendants and 191 copies were used for analysis. To analyze the collected data, factor, reliability analysis and T test were conducted. The results of this study are as follows. First, American flight attendants were higher in service recovery performance than Japanese flight attendants. Second, there were differences in service behaviors between two groups showing that American flight attendants are higher in extra-role and role-prescribed service behaviors. There was no difference in cooperation. Third, out of 4 dimensions of service quality, American flight attendants are higher in all of 4 dimensions which are tangible, reliability/assurance, responsiveness, and empathy.