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- 저자 : 박영서 ( Young Seo Park ) (검색결과 160 건)
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패턴 최적배치를 위한 유전 알고리즘의 응용에 관한 연구
박영서,장승호,허유,Park, Young-Seo,Jang, Sung-Ho,Huh, You 한국섬유공학회 2003 한국섬유공학회지 Vol.40 No.4
A good algorithm for optimum packing of materials is very important in that batch production of small quantities that requires a quirk response of the manufacturing system to the change in the market demand turns out to be an industrial trend. In spite of many researches on optimum packing, performance of pattern packing on the basis of computer aided automation if still inferior to that by manual operation from the material utilization point of view. In this paper we develop a software program based on genetic algorithm that is known to be proper for searching an optimal solution and try to ascertain whether the algorithm adopted is suitable and effective for improving the efficiency of the pattern laying process for apparel manufacturing. The pattern laying problem is divided into two parts: to determine a priority order of pattern and to achieve a high efficiency of materials by the order already given. For determining the order of placing patterns, a genetic algorithm was applied and for a pattern location a path pursuit algorithm. The results show that the pattern packing software, consisting of the genetic alogorithm and the path pursuit strategy used In this study, is one of the ways suitable for making the pattern laying process efficient and consistent, which was assessed indirectly by the random search method. The simulation reveals that the pattern laying efficiency of the method adopted in this study is about 85%, independent of the number of the pattern elements.
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Bacillus alcalophilus AX2000 유래 xylanase 유전자 (XynT)의 Cloning과 염기서열 분석
박영서,Park Young-Seo 한국생명과학회 2005 생명과학회지 Vol.15 No.5
Xylanase를 생산하는 알칼리 내성 Bacillus alcalophilus AX2000의 chromosomal DNA로부터 xylanase 유전자를 cloning하여 그 염기배열 순서를 결정한 다음 이로부터 유전자 발현에 관련된 구조를 분석하였다. Xylanase 유전자의 cloning을 위해 제한효소 PstI으로 절단한 B. alcalophilus AX2000의 chromosomal DNA와 pUC19을 ligation 시켜 E. coli $DH5\alpha$에 형질전환시킨 후 형질전환체 중에서 xylanase 활성을 나타내는 재조합 plasmid pXTY99를 분리하였다. 재조합 plasmid pXTY99은 pUC19의 PstI 부위 내에 7kb의 외래 DNA가 삽입 되 었다. Cloning된 xylanase 유전자(xynT)의 염기배열을 분석한 결과 유전자의 크기는 1,020 bp이었고 이는 340개의 아미노산으로 구성된 분자량 40 kDa의 poly-peptide를 coding하고 있었다. 이 염기배열은 AUG 개시 codon으로부터 각각 259와 282 base상류에 TACAAT의 -10 box와 GTTCACA인 -35 box로 추정되는 염기배열이 존재하였으며 ribosome 결합부위가 존재하였다. B. alcalophilus AX2000의 xylanase와 아미노산배열의 유사성이 가장 높은 xylanase는 Bacillus sp. N137과 B. stearothemophilus 21 유래의 xylanase로 각각 $61\%$와 $59\%$의 유사성을 나타내었다. A gene coding for xylanase from alkali-tolerant Bacillus alcalophilus AX2000 was cloned into Escherichia coli $DH5\alpha$ using pUC19. Among 2,000 transformants, one transformant showed clear zone on the detection agar plate containing oat-spells xylan. Its recombinant plasmid, named pXTY99, was found to carry 7.0 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynT) was determined, xynT gene was found to consist of 1,020 base-pair open reading frame coding for a poly-peptide of 340 amino acids with a deduced molecular weight of 40 kDa. The coding sequence was preceded by a putative ribosome binding site, and the transcription initiation signals. The deduced amino acid sequence of xylanase is similar to those of the xylanases from Bacillus sp. Nl37 and B. stearothermophilus 21 with $61\%$ and $59\%$ identical residues, respectively.
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Bacillus Pumilus TX703 유래 Xylanase의 활성에 관여하는 아미노산 잔기의 확인
박영서,Park Young-Seo 한국생명과학회 2005 생명과학회지 Vol.15 No.4
The purified xylanase from Bacillus pumilus TX703 was modified with various chemical modifiers to determine the active sites of the enzyme. Treatment of the enzyme with group-specific reagents such as carbodiimide or N-bromosuccinimide resulted in complete loss of enzyme activity. These results assumed that these reagents reacted with glutamic acid or aspartic acid and tryptophan residues located at or near the active site. In each case, inactivation was performed by pseudo first-order kinetics. Inhibition of enzyme activity by carbodiimide and W-bromosuccinimide showed non-competitive and competitive inhibition type, respectively. Addition of xylan to the enzyme solution containing N-bromosuccinimide prevented the inactivation, indicating the presence of tryptophan at the substrate binding site. Analysis of kinetics for inactivation showed that the loss of enzyme activity was due to modification of two glutamic acid or aspartic acid residues and single tryptophan residue. Bacillus pumilus TX703으로부터 xylanase를 정제한 후 효소의 활성부위를 조사하기 위하여 여러 가지 화학수식제를 사용하여 효소활성의 저해도를 측정하였다. 여러 가지 화학수식제 중에서 carbodiimide와 N-bromosuccinimide가 효소활성을 완전히 저 해시 켜 glutamic acid 또는 aspartic acid 잔기와 tryptophan 잔기가 효소의 활성부위에 관여하리라 추측되었다. 각각의 경우에 효소 실활은 수식제의 첨가농도에 따라 pseudo first-order kinetics 양식을 보여주었으며, car-bodiimide와 N-bromosuccinimide는 각각 비경쟁적 저해와 경쟁적 저해방식을 나타내었다. 기질첨가에 의한 효소활성 보호실험을 통하여 tryptophan 잔기가 기질결합부위라 판단되었다. 효소실활속도의 분석에 의해 효소활성에는 2개의 glutamic acid 또는 aspartic acid 잔기와 1개의 tryptophan 잔기가 관여하는 것으로 나타났다.
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박영서 ( Young Seo Park ),최혁준 ( Hyuk Joon Choi ),최태현 ( Tae Hyun Choi ) 한국산업식품공학회 2009 산업 식품공학 Vol.13 No.4
목이버섯으로부터 항혈전활성과 항혈소판응집활성을 지니는 물질을 추출하여 추출물의 혈행개선활성을 조사하였다. 건조 목이버섯을 0.1 N NaOH, methanol, ethanol 등의 용매를 사용하여 추출하여 각 추출물의 항혈전활성을 activated partial thromboplastin time, thrombin time과 prothrombin time 값으로 측정한 결과 methanol 용해성 분획이 각각 100, 124, 54 s로 조사한 분획 중에서 가장 높은 활성을 나타내었다. 활성 분획을 DEAE-Sepharose CL6B ion exchange column chromatography와 Sephacryl 400-HR gel permeation column chromatography로 정제하였으며 활성물질은 분자량이 150 kDa 이상인 다당류이며 mannose가 주요 구성당으로 되어 있는 xyloglucomannan의 복합다당체인 것으로 확인되었다. 정제된 다당류의 항혈전활성은 thrombin 활성을 저해하기 때문인 것으로 해석되었다. Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by H2O. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.
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박영서 ( Young Seo Park ),장재권 ( Jae Kweon Jang ),최영진 ( Young Jin Choi ),정명수 ( Myong Soo Chung ),박훈 ( Hoon Park ),심건섭 ( Kun Sub Shim ) 한국산업식품공학회 2009 산업 식품공학 Vol.13 No.1
양파 착즙액에 팽화 홍삼 추출액의 첨가량을 달리하면서 Pediococcus pentosaceus KC-007을 이용하여 유산발효시킨 후 발효액의 특성과 생리활성을 조사하였다. 양파 착즙액에 팽화 홍삼 추출액을 각각 0.5, 1, 2, 4%(v/v) 첨가한 후 배양시간에 따른 유산균 생균수를 측정한 결과 팽화 홍삼 추출액의 첨가량에 관계없이 발효 24시간에서 가장 높은 생균수를 나타내었다. 발효액의 산도는 팽화 홍삼의 첨가량에 관계없이 모든 시료구에서 발효가 진행됨에 따라 증가하였으며 발효액의 pH는 발효 36시간까지 유의적으로 감소하는 경향을 나타내었다. 발효액의 환원당은 발효 24시간까지는 환원당 함량이 감소하다가 그 이후부터는 유의적인 변화를 나타내지 않았다. 발효액의 전자공여능과 아질산염 소거능은 1%(w/v) 이상의 팽화 홍삼 추출액을 양파 착즙액에 첨가하여 발효하였을 때 가장 높은 수치를 나타내었고 종합적 기호도는 1%(w/v)의 팽화 홍삼 추출액을 첨가하였을 때 가장 좋았으므로 양파 착즙액에 팽화 홍삼 추출액을 첨가하여 발효할 경우에는 팽화 홍삼 추출액을 1%(w/v) 첨가하는 것이 가장 적합한 것으로 확인되었다. Onion juices supplemented with different concentrations of puffed red ginseng extract were fermented using Pediococcus pentosaceus KC-007 and their biologically functional properties were investigated. When onion juices were supplemented with puffed red ginseng extract at the concentration of 0.5, 1, 2, and 4% (v/v) each, viable cell number of lactic acid bacteria was the highest at 24 hr of fermentation in all samples. The titratable acidity increased as the fermentation proceeds irrespective of the added amount of red ginseng extract, and the pH of fermentation broth decreased until 36 hr of fermentation. The reducing sugar of fermentation broth decreased until 24 hr of fermentation and did not change thereafter. The electron donating ability and nitrite scavenging ability were highest when red ginseng extract was added at the concentration of more than 1% (w/v). The overall acceptance in sensory evaluation was the best when red ginseng extract was added at the concentration of 1% (w/v). From these results, it is confirmed that the optimum concentration of puffed red ginseng extract for the lactic acid fermentation of onion juice was 1% (w/v).
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