패턴 최적배치를 위한 유전 알고리즘의 응용에 관한 연구

박영서,장승호,허유,Park, Young-Seo,Jang, Sung-Ho,Huh, You 한국섬유공학회 2003 한국섬유공학회지 Vol.40 No.4

A good algorithm for optimum packing of materials is very important in that batch production of small quantities that requires a quirk response of the manufacturing system to the change in the market demand turns out to be an industrial trend. In spite of many researches on optimum packing, performance of pattern packing on the basis of computer aided automation if still inferior to that by manual operation from the material utilization point of view. In this paper we develop a software program based on genetic algorithm that is known to be proper for searching an optimal solution and try to ascertain whether the algorithm adopted is suitable and effective for improving the efficiency of the pattern laying process for apparel manufacturing. The pattern laying problem is divided into two parts: to determine a priority order of pattern and to achieve a high efficiency of materials by the order already given. For determining the order of placing patterns, a genetic algorithm was applied and for a pattern location a path pursuit algorithm. The results show that the pattern packing software, consisting of the genetic alogorithm and the path pursuit strategy used In this study, is one of the ways suitable for making the pattern laying process efficient and consistent, which was assessed indirectly by the random search method. The simulation reveals that the pattern laying efficiency of the method adopted in this study is about 85%, independent of the number of the pattern elements.

Bacillus Pumilus TX703 유래 Xylanase의 활성에 관여하는 아미노산 잔기의 확인

박영서,Park Young-Seo 한국생명과학회 2005 생명과학회지 Vol.15 No.4

The purified xylanase from Bacillus pumilus TX703 was modified with various chemical modifiers to determine the active sites of the enzyme. Treatment of the enzyme with group-specific reagents such as carbodiimide or N-bromosuccinimide resulted in complete loss of enzyme activity. These results assumed that these reagents reacted with glutamic acid or aspartic acid and tryptophan residues located at or near the active site. In each case, inactivation was performed by pseudo first-order kinetics. Inhibition of enzyme activity by carbodiimide and W-bromosuccinimide showed non-competitive and competitive inhibition type, respectively. Addition of xylan to the enzyme solution containing N-bromosuccinimide prevented the inactivation, indicating the presence of tryptophan at the substrate binding site. Analysis of kinetics for inactivation showed that the loss of enzyme activity was due to modification of two glutamic acid or aspartic acid residues and single tryptophan residue. Bacillus pumilus TX703으로부터 xylanase를 정제한 후 효소의 활성부위를 조사하기 위하여 여러 가지 화학수식제를 사용하여 효소활성의 저해도를 측정하였다. 여러 가지 화학수식제 중에서 carbodiimide와 N-bromosuccinimide가 효소활성을 완전히 저 해시 켜 glutamic acid 또는 aspartic acid 잔기와 tryptophan 잔기가 효소의 활성부위에 관여하리라 추측되었다. 각각의 경우에 효소 실활은 수식제의 첨가농도에 따라 pseudo first-order kinetics 양식을 보여주었으며, car-bodiimide와 N-bromosuccinimide는 각각 비경쟁적 저해와 경쟁적 저해방식을 나타내었다. 기질첨가에 의한 효소활성 보호실험을 통하여 tryptophan 잔기가 기질결합부위라 판단되었다. 효소실활속도의 분석에 의해 효소활성에는 2개의 glutamic acid 또는 aspartic acid 잔기와 1개의 tryptophan 잔기가 관여하는 것으로 나타났다.

Bacillus alcalophilus AX2000 유래 xylanase 유전자 (XynT)의 Cloning과 염기서열 분석

박영서,Park Young-Seo 한국생명과학회 2005 생명과학회지 Vol.15 No.5

Xylanase를 생산하는 알칼리 내성 Bacillus alcalophilus AX2000의 chromosomal DNA로부터 xylanase 유전자를 cloning하여 그 염기배열 순서를 결정한 다음 이로부터 유전자 발현에 관련된 구조를 분석하였다. Xylanase 유전자의 cloning을 위해 제한효소 PstI으로 절단한 B. alcalophilus AX2000의 chromosomal DNA와 pUC19을 ligation 시켜 E. coli $DH5\alpha$에 형질전환시킨 후 형질전환체 중에서 xylanase 활성을 나타내는 재조합 plasmid pXTY99를 분리하였다. 재조합 plasmid pXTY99은 pUC19의 PstI 부위 내에 7kb의 외래 DNA가 삽입 되 었다. Cloning된 xylanase 유전자(xynT)의 염기배열을 분석한 결과 유전자의 크기는 1,020 bp이었고 이는 340개의 아미노산으로 구성된 분자량 40 kDa의 poly-peptide를 coding하고 있었다. 이 염기배열은 AUG 개시 codon으로부터 각각 259와 282 base상류에 TACAAT의 -10 box와 GTTCACA인 -35 box로 추정되는 염기배열이 존재하였으며 ribosome 결합부위가 존재하였다. B. alcalophilus AX2000의 xylanase와 아미노산배열의 유사성이 가장 높은 xylanase는 Bacillus sp. N137과 B. stearothemophilus 21 유래의 xylanase로 각각 $61\%$와 $59\%$의 유사성을 나타내었다. A gene coding for xylanase from alkali-tolerant Bacillus alcalophilus AX2000 was cloned into Escherichia coli $DH5\alpha$ using pUC19. Among 2,000 transformants, one transformant showed clear zone on the detection agar plate containing oat-spells xylan. Its recombinant plasmid, named pXTY99, was found to carry 7.0 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynT) was determined, xynT gene was found to consist of 1,020 base-pair open reading frame coding for a poly-peptide of 340 amino acids with a deduced molecular weight of 40 kDa. The coding sequence was preceded by a putative ribosome binding site, and the transcription initiation signals. The deduced amino acid sequence of xylanase is similar to those of the xylanases from Bacillus sp. Nl37 and B. stearothermophilus 21 with $61\%$ and $59\%$ identical residues, respectively.

광학용 플라스틱 산업 시장동향

박영서,Park, Yeong-Seo 한국광산업진흥회 2004 광산업정보 Vol.20 No.-

일본 JST의 고기능 기반 DB 구축 사업 소개

박영서,Park, Yeong-Seo 한국과학기술정보연구원 2000 지식정보인프라 Vol.1 No.-

복분자의 유산발효와 생리활성 평가

박영서 ( Park Yeong Seo ),장학길 ( Jang Hag Gil ) 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

The puree of Rubus coreanus was fermented using lactic acid bacteria and its biological activities were examined. Lactobacillus acidphilus KCCM 32820, L. casei KCCM 12452. Lactococcus lactis subsp. lactis KCCM 40104, and Streptococcus thermophilus KCCM 40430 were used as a single or mixed starter for the lactic acid fermentation, and their cultures at the late logarithmic growth phase were inoculated to final concentration of 2% (v/v). L. casei fermented the puree of Rubus coreanus best when used as a single starter, and the culture of L. casei and L. lactis with the inoculation ratio of one to one showed the highest fermentation activity when used as a mixed starter. However, the fermented broth of the puree of Rubus coreanus using L. acidophilus and S. thermophilus showed the best results in the sensory evaluation. The optimal lactic acid fermentation conditions were as follows; the concentration of oligosaccharide added was 1% (w/v), pH of puree and fermentation temperature were 4.0 and 37℃. respectively, and fermentation time was 72~96 hours. Glucose and fructose were major free sugars, and the content of lactic acid was 698.2㎎/100g in the fermented broth. The fermented broth of the puree of Rubus coreanus showed the electron donating ability and nitrite scavenging ability with the value of 69% and 38.3% at pH 1.2, respectively. SOD-like activity and inhibitory activity on xanthine oxidase were also found in the fermented broth were examined, it showed the highest growth inhibitory activity against Escherichia coli O-157:H7, and also contained antimicrobial activities against Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus.

흑미가루 복합분의 이화학적 특성과 스펀지 케이크 품질특성

박영서(Young-Seo Park),장학길(Hak-Gil Chang) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.4

흑미가루 첨가비율(5-30%)에 따른 복합분의 이화학적 특성, 호화특성, mixograph 특성 및 스펀지 케이크의 제조적성을 측정하고, 이들 특성 간의 상관관계를 구명하고자 일련의 실험을 실시하였다. 흑미가루 첨가량이 증가함에 따라서 alkaline water retention capacity, Pelshenke value, sedimentation value는 증가하였으나 water holding capacity는 감소하는 경향을 보였다. Mixograph peak time, height, width at peak와 Rapid Visco Analyser peak, minimum viscosity도 감소하였다. 스펀지 케이크 반죽의 pH와 비중은 증가하였지만 케이크의 부피는 감소하는 경향을 보였으며, 케이크의 경도는 증가하였다. In this study the replacement of wheat flour with 5 to 30% black rice flour was tested to examine its effect on sponge cake making properties. We evaluated the effects of the substitutions on various quality parameters of the cake, such as physicochemical and rheological properties, cake making quality, and texture characteristics. The water holding capacity value increased with increasing amounts of black rice flour, whereas the alkaline water retention capacity, Pelshenke value and sedimentation value decreased. The mixograph peak time and peak height, and the Rapid Visco Analyser peak, minimum, and final viscosity decreased with the addition of black rice flour. Increasing proportions of black rice flour resulted in reduced loaf volume. However, replacing 15% of the wheat flour with black rice flour did not significantly affect the characteristics of the sponge cake. The textural characteristics of the sponge cake crumb were influenced by the addition of black rice flour, and sponge cakes containing the additives showed increases in hardness, gummines, and chewiness.

광고 메시지의 기호학적 분석

박영서(Park, Young Seo),김문기(Kim, Moon Gi) 한국디지털디자인협의회 2014 (사)한국디지털디자인협의회 conference Vol.2014 No.5

부모 공감과 청소년의 삶의 만족도 간의 관계에서 또래 애착과 사회적 자기효능감의 이중매개효과: 코로나19 상황을 중심으로

박영서(Youngseo Park),최진아(Jinah Choi) 한국아동심리재활학회 2023 놀이치료연구 Vol.27 No.2

목이버섯 추출물로부터 항혈전물질의 정제와 항혈전효과

박영서 ( Young Seo Park ),최혁준 ( Hyuk Joon Choi ),최태현 ( Tae Hyun Choi ) 한국산업식품공학회 2009 산업 식품공학 Vol.13 No.4

목이버섯으로부터 항혈전활성과 항혈소판응집활성을 지니는 물질을 추출하여 추출물의 혈행개선활성을 조사하였다. 건조 목이버섯을 0.1 N NaOH, methanol, ethanol 등의 용매를 사용하여 추출하여 각 추출물의 항혈전활성을 activated partial thromboplastin time, thrombin time과 prothrombin time 값으로 측정한 결과 methanol 용해성 분획이 각각 100, 124, 54 s로 조사한 분획 중에서 가장 높은 활성을 나타내었다. 활성 분획을 DEAE-Sepharose CL6B ion exchange column chromatography와 Sephacryl 400-HR gel permeation column chromatography로 정제하였으며 활성물질은 분자량이 150 kDa 이상인 다당류이며 mannose가 주요 구성당으로 되어 있는 xyloglucomannan의 복합다당체인 것으로 확인되었다. 정제된 다당류의 항혈전활성은 thrombin 활성을 저해하기 때문인 것으로 해석되었다. Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by H2O. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.