감자 바이러스 무균종묘의 대량생산과 플러그화에 관한 기초 연구

朴良門,蘇寅燮,柳長杰,姜奉均 韓國作物學會 1997 Korean journal of crop science Vol.42 No.6

감자의 생장점 배양을 통하여 생산된 소량의 소괴경(microtuber)을 경삽하여 균일하게 생장된 플러그우량묘를 대량생산할 수 있는 기술을 개발하기 위하여 삽수를 정단 및 기부부위로 구분하고 NAA 및 IAA 10 mg /1 용액에 2시간 침지하여 cocopeat, vermiculite, perlite, peatmoss 혼용배지를 이용한 삽목과 온로장 처리 ,포장정식후 괴경형성효을에 대하여 시험한 결과를 요약하면 다음과 같다. 1. 감자의 소괴경 유내 줄기를 이용한 플러그 삽목시 고체배지는 cocopeat, vermiculite, perlite, peatmoss를 이용한 혼합배지에서 플러그의 형성율이 양호하게 나타났다. 2. 고온장일조건(20℃ 이상, 16시간의 일장)에서는 지상부생육 및 지하부발근이 양호하였으나, 외부방치구에서는 발근이 부진하고 복지가 발생하여 소괴경이 형성되었다. 3. 지상, 지하부 생체중은 생장조절제 전처리시 NAA 10mg/1 및 IAA 10mg/1 간에 차이가 없었다. 4. 삽수부위별로는 삽목묘의 초기생육은 초장에서는 비슷한 경향이었고, 그 외 엽수, 근수, 근장 등의 형질은 정단부위묘가 양호하였지만, 포장정식 후에는 큰 차이가 없었다. 5. 삽목묘를 봄재배하여 괴경형성여부를 시험한 결과 초장은 70~80cm, 복지수 10여개, 괴경수는 7~8개가 형성되었다. This experiment was carried out to develop the mass propagation system for producing plug plantlets using stem cuttings of virus-tree microtubers in potato. Cocopeat, vermiculite, perlite and peatmoss were combined and used as plug nursery media to find out the best combination suitable for the growth of seedlings derived from microtubers. Seedling growth was favored in high temperature (above 20~circC ) and a long-day photoperiod(above 16 hours) condition, while stolons and microtubers formed in outdoor condition. Shoot and root multiplication was not affected by NAA 10mg /1 or IAA 10mg /1 treatment. At the early growth stage of plug plantlets, the number of leaves and roots and the length of root increased significantly when nodes from the upper (near to apex) part of shoots rather than from basal part were taken. But after transplanting, these differences among these characters were not observed. At ninety days after transplanting the plug plantlets in spring time, plant was around 70 to 80cm in height, and the number of stolons and tubers were ten and seven, respectively.

主要作物의 葉汁酸度에 관한 硏究(1)

朴良門 제주대학교 1980 논문집 Vol.12 No.-

The pattern of pH changes in the leaf sap with changes of nutrient solution and soil pH were invstigated. Further studies on the relation between sap pH and the growth and yield of major crops were made. The results obtained are summarized as follows; 1. The sap pH of the terminal, basal and middle leaves of fifteen crops was measured. The average pH of the three different types of the leaves was the same in each crop. 2. Sap pH of corn, soybean and sweet potato showed low acidity in the morning and tended to strengthen in the afternoon. 3. No significant differences of sap pH exsisted between five varieties of sweet potatos tested. 4. Sap pH was slightly affected by the stage of crop growth, there's being tendency towards less acidity in the later stages of growth. A pH difference occured between leaves attached at different places up the stem. 5. Sap pH measurements on major crops(37 species) and weeds (16 species) showed a pH range of 3.83 ∼7.31. When classified into six groups : 19 species had a pH of 5.6 ∼6.0, 17 species a pH of 6.1∼6.5, four species (grape, ginkgo, persimmon, rumex) were below pH 4.5 and eight species (pumpkin, rice, soybean, ramie, perilla, rape, alfalfa and speedwell) showed sap pH above 6.6. 6. When crops (rice, corn, soybean, sweet potato, pumpkin) were cultivated in nutrient solutions different pH, strongly acid and alkaline solutions were neutralized by growth. Dispite this little change was observed in plant leaf sap pH.

野草의 生態的 特性

朴良門 제주대학교 1976 논문집 Vol.8 No.-

The work has taken place in Suweon, Using plants were smartweed (polygonum Conspicum Nakai), dayflower (commellina communis L.), stichwort (Alsine Grimn var. undulata ohwi), larger crabgrass (Digitaria Adscendis Henr.), Common purslane (portulaca oleracea L.), amaranth (Amaranthus Asendens Loisel.), umbrella plant (Cyerus Differmis L.), water foxtail(Alopeculus Aequalis sobel var. amurensis ohwi.), green foxtail (setaria viridisp. Beauv.), siegesbeckia (siegesbekia glabresens Makino), oriental cocklebur (Xnthium L.), goose foot (chenopodium Album L.), wild oat (Abena fatua L.), shepherd's purse (Capsella Bursa-pastoris Medicus), fielder corn (Ixeris stolonifera Maxim), bindweed (Calystegia japonica choisy), araceao (pinellia ternata Breitenbach), Curly dock (Rumax crispus L.), flat sedge (Cyperus micr-oiria steud.), bog pondweed (potamoton distincts A. Bennet), dandelion (Taraxacum Dahl.) and squill (scilla scilloides Druce.), Result were as follow : (1) Wintering weedy plants were emergencing from deep place than emergence in Summer. (2) A knowledge of the manner of weedy plants and of the agencies responsible for their dispersal forma a basis for prevention of introduction and spread. Unfortunately for the agriculturist, many weedy species reproduce and spread vegetatively as by seed. (3) The order of drought resistant is Common purslane=dayflower>larger crab-grass>amaranth>wild oat>water foxtail=barley>smartweed=oriental cocklebur. (4) Degree of covering depth were influence to control of assimilation and consumption of nutrients by respiration.

재래종 동부 수집종에 대한 연구

박양문,현승원 제주대학교 1984 논문집 Vol.17 No.-

Seven varieties of local cowpea (Vigna sinensis) collected from the mainland were examined to evaluate agronomic characters, yield components and productivity. Average flowering date of local cowpea was the 8th of August with earliest variety Gamaegwidongbu and latest variety Soebbuldongbu. Leaf area index (L.A.I.) and suppression of weeds were 7.8 and 86% respectively, indicating vigorous character of cowpea. Maturing time occured on the 14th of September and Soebbuldongbu variety showed latest maturing on the 30th of September. Grain yield per 10a. (kg/10a.) was 288.9kg and highest variety was Hindongbu (41 7.4kg). Higher podding ratio (74.5%) was observed when compared to soybean (25-40%) and leaf sap pH was 6.08. Mean stem length of cowpea was 320 cm, and variety with longest stem was Pangyodongbu of 421cm. Pod length was 17.2 cm and longest in Hindongbu (22.2cm), shortest variety was Najudongbu (12.8cm). Soebbuldongbu showed longest main root length of 181cm and root penetrating depth was upto 65cm from the top soil, and mean root length of cowpea was 135crn. Average number of pod per hill was 28.7 the greatest number was found in Mungokdongbu (39). Num-ber of seed per pod; 16.6 seeds, weight of 1 liter; 754g and weight of 100 grain was 17.4g. Mean seed size was 8.5 mm x 6.4.mm. The seed coat color varies greatly between varieties: brown (Soebbuldongbu), white (Hindongbu, Najudongbu), black (Gamaegwidongbu), pale gray (Pangyodongbu), gray (Mungok-dongbu) and yellowish white color (Suchondongbu).

在來種 옥수수品種 粒色의 優劣性

朴良門 제주대학교 1992 논문집 Vol.34 No.-

. Ten varieties (caassified by grain colors) of local corn were collected from Cheju-do and mainland. The corn grains have a wide range of colors, white,yellow,yellowish, gray,violet,brown,dark brown,black brown, red and black. Open pollinated results(changes of grain colors) were as follows: When yellow corn(seed parent) is pollinated by white corn(pollen parent), the grains are yellow but are lighter in color and often capped with white. When white corn is pollinated by yellow corn, the grains are yellow. When white corn is pollinated by black corn, the grains are black. When yellow corn is pollinated by black corn, the grains are black. When yellow corn is pollinated by violet corn, the grains are black.

植物汁液의 反應에 대한 土壤酸度의 影響

朴良門 제주대학교 1979 논문집 Vol.11 No.-

An original soil pH of 5.3 was changed to 5.5, 6.5 and 7.5 by applying calcium carbonate. The resulting various in growth, yield and leaf sap pH were observed in corn (bantam), soybean (hill) and buckwheat (native). Sample were taken at 3 week intervals during the growing stage 5 times a day every 3 hours starting at 6 a.m. At the same intervals another 8 species were investigated a t 9 a.m., noon and 3 p.m. irrespective of growth condition. Average plant leaf sap pH occured at noon, plant sap acidity seemed to be changed by some factors including plant species, sampling time of the day, growing stage and soil acidity. The average pH of leaf sap of corn was 5.52, of baryard grass 5.85, of buckwheat 4.83, soybean 6.64, garden pea 5.99, sweet potato 5.98, oriental cocklebur 5.99, speedwell 5.98, siegesbekia 7.13, and common nightshade 6.79. Soil acidity influenced the pH of leaf sap very slightly. Also differences leaf sap pH seemed to affect the length of time taken for germination, plant length, heading date, ripening period, weight of 100 grain and yields in the same plant.

제주지방 재래종 속품종에 관한 연구

박양문,송창길,강승진 제주대학교 1985 논문집 Vol.20 No.-

Thirty-four varieties of local Italian millet(Sefario italica) collected from Cheju-do area were examined evaluate agronomic characters, yield components and productivity Average heading date of local Italian millet was the 18th of August. Maturing time occured on the 27th of September and six varieties (CIM-1. 5. 12. 14, 21 and 33) showed latest maturing on the 30th of September Grain yield per 10a (kg/10a.) was 173.7kg and highest variety was CIM-2 (242.4kg). Mean culm length of Italian millet was 82.9cm. and variety with longest culm was CIM-16 of 89.6cm. Spike length was 21.6cm and longest in CIM-1(33.5cm). shortest variety was CIM-33( 13.6cm) Bristle length was 3.0mm and longest in CIM-3(8.1mm). shortest variety was CIM-21(1.5mm). Shape of spikes were clostridial(l9). branches(l2). cylindrical(2) and plectridial(1) in 34 varieties Weight of 1 liter. 648g and weight of 1000 grains was 2.6g.

主要作物의 葉汁酸度에 관한 硏究(2)

朴良門 제주대학교 1981 논문집 Vol.13 No.-

土壤酸度 5.3을 炭酸石灰를 施用하여 5.5, 6.5, 및 7.5로 矯正하고 陸稻, 大麥, 小麥, 胡麥, 옥수수, 小豆, 동부, 菜豆, 무우, 고추, 호박 등을 栽培하여 葉汁酸度와 收量에 미치는 영향을 調査하여 다음과 같이 綜合하였다. 作物의 生育時期에 따르는 葉汁酸度의 變化는 크지 않았으나 生育後期로 갈수록 酸度가 强해지는 傾向을 보였다. 土壤酸度를 달리하여 陸稻外 10種의 作物을 栽培한 結果 pH 7.5까지 높아지는 경우까지 收量의 增加를 보인 것은 고추와 호박이었으며, 陸稻, 大麥, 小麥, 胡麥, 옥수수, 小豆, 동부, 菜豆, 무우 등은 pH 6.5 水準까지 增收를 보였다. The soil pH of the field was rectified to 5.5, 6.5 and 7.5 with calcium carbonate. And the influence of soil pH on the plant sap pH and the yield was investigated in 11 crops : upland rice, barley, wheat, rye, corn, small red bean, cowpea, garden bean, radish pepper and pumpkin. The reults are as follows : Sap pH was silightly affected by the stage of crop growth, there's being atendency towards less acidity in the later stages of growth. Different levels of soil pH affected crop yield. Only two crops(pepper and pumpkin) showed tolerance of high soil pH, and increased their yield up to pH 7.5. The rest of the crops(upland rice, barley, wheat, rye, corn, small red bean, cowpea, garden bean and radish) increased their yield only up to a soil ph of 6.5.

黃酸處理에 依한 種皮破傷에 關한 硏究

朴良門 제주대학교 1977 논문집 Vol.9 No.-

A study was conducted to invetigate the effect of chemical. treatment on the germination of five woody plant seeds; Honey lovust(Gleditschia japonica Miquel Rcraiensis Nakai),Japanese wisteria (milletia japonica AGray), Camellia(Camellia japonicaL), Neolitsea sericea(Blume)koidzumi and tea tree (Theasinensis L.var.Bohea(L)K>Koch). Sulfric acid(95%W/W)and calcium oxide were used for chemical treatment and soaking time of seeds in the acid solutions were 0.0,0.2,0.4,0.5,0.6,1.0,1.5,2.0,3.0,5.0 minutes respectively. The results obtained are summarized as follows; 1) Sulfuric acid treatment significantly increased the germinaton rate of seeds highest germin- ation rate was found in Honey locust(92.0%)and Neolitsea sericea(17.3%),Cameliia(10.6%), Tea tree(4.0%),Japanese wisteria(2.7%)respectively. 2) It is possible that the sulfurhc acid treatment of plant seeds will both shorten the legth of germination and increase the germination rate. 3) The rate of hard seeds in Leguminosae was about 15.3%.

아세아 농업계 대학간 협력의 필요성 및 전망

박양문 濟州大學校 亞熱帶農業硏究所 1988 亞熱帶農業硏究 Vol.5 No.-