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DGGE를 이용한 대청호 수화 발생시기의 세균군집 분석
고소라,박성주,안치용,최애란,이정숙,김희식,윤병대,오희목,Ko So-Ra,Park Seong-Joo,Ahn Chi-Yong,Choi Aeran,Lee Jung-Sook,Kim Hee-Sik,Yoon Byung-Dae,Oh Hee-Mock 한국미생물학회 2004 미생물학회지 Vol.40 No.3
대청호에서 수화 발생시기인 2003년 7월에서 10월까지 분자생태학적 방법의 하나인 DGGE (denaturing gradient gel electrophoresis)를 이용하여 시간에 따른 세균군집구조의 변화를 연구하였다. 조사기간 동안 출현한 식물플랑크톤을 형태학적으로 분류한 결과 cyanobacteria, 규조류 및 녹조류가 발견되었고, 이 중 Microcystis, Chroococcus, Oscillatoria, Phormidium 속이 크게 우점하였다. 16S rDNA의 DGGE pronto 분석에 의하여 Microcystis flos-aquae와 Oscillatoria spp.가 우점하는 것으로 확인되었으며, Aphanizomenon flos-aquae는 8월 중순에 우점하는 것으로 확인되었다. DGGE profile을 토대로 cluster analysis를 적용하여 다양한 미생물 군집의 유사성을 비교한 결과, 9월 2일의 미생물 군집이 다른 시기의 시료와 확연히 다른 그룹으로 구분되었다. 결과적으로 분자 생물학적 방법은 형태적 분석방법과 유사한 결과를 보였으며, 일부 세균의 분포 및 변화, 미생물 군집의 유사성 등에 대한 추가적 정보를 제공하였다. The change of bacterial communities during cyanobacterial bloom was analyzed by DGGE in Daechung Reservoir from July to October in 2003. The traditional morphological analysis showed that the genera of Microcystis, Chroococcus, Oscillatoria, and Phormidium were dominated. The most frequent band in the DGGE profile by 16S rDNA sequence analysis was identified as Microcystis flos-aquae and the cyanobacterial bloom was peaked on September 2. Oscillatoria spp. were also identified and Aphanizomenon flos-aquae dominated in the middle of August. Judging from the analysis of the digitalized DGGE profiles using the cluster analysis technique, the microbial community on September 2 was considerably different from others. Consequently, it seems that the gene fingerprinting method can give not only the similar results to the traditional morphological method but also additional information on the bacterial species and similarity among the examined microbial communities.
위험 음주자의 선별을 위한 한국어판 Alcohol Use Disorders Identification Test(AUDIT-K)의 최적 절단값
조근호(Keun-Ho Joe),채숙희(Suk-Hi Chai),박애란(Aeran Park),이해국(Hae-Kook Lee),신임희(Im-Hee Shin),민성호(Seong-Ho Min) 한국중독정신의학회 2009 중독정신의학 Vol.13 No.1
Objects:Through this study, we intended to set up new cut-off score for the screening of hazardous drinking using the Korean version of alcohol use disorders identification test (AU-DIT-K). Methods:A total of 1,100 subjects were enrolled in this study. Questionnaires about drinking patterns, AUDIT-K, CAGE and NAST (National Alcohol Screening test) were col-lected. The cut-off scores of AUDIT-K for hazardous drinking were calculated and determined by area under the Receiver Operating Characteristic (ROC) curve. Pearson’s correlation test was applied to validate the test and re-test data. Results:Suggestible cut-off scores based on drinking frequency and amount, the AUDIT-K for screening the hazardous drinking would be 11 for men and 8 for women. However, we further proposed the CAGE 1 point as a reference value and concluded that cut-off scores of 10 for men and 6 for women were more appropriate in screening of hazardous drinking in Korean subjects. According to these scores recommended limits for normal drink-ing behavior among Koreans would 13 standard drinks per week for men and 6 standard drinks per week for women. Conclusion:The lower cut-offs of 10 for men and 6 for women suggest that nationwide a more vigorous effort in screening for hazardous drinking is needed in order to prevent alcohol abuse and hazardous consequences.
이해국(Hae Kook Lee),이명수(Myung-Soo Lee),기선완(Seon Wan Ki),최삼욱(Sam-Wook Choi),조근호(Keun Ho Joe),박애란(Aeran Park),정인원(In-Won Chung),김석범(Seok Beom Kim),이정태(Chung Tai Lee),권용실(Yong Sil Kweon) 대한사회정신의학회 2007 사회정신의학 Vol.12 No.2
Alcoholism is complex primary physiological disease with unique therapeutic interventions, and neither a primary behavior disorder nor ay symptomatic manifestation of any other disease process. And, it is highly prevalent disease with important impact on public health perspective. Therapeutic approach for alcoholism need to be organized by integrative and comprehensive systems, because that development and course of alcoholism are influenced by complex interaction between biological and environmental factors. We tried to developing effective intervening systems for alcoholism in seoul by evaluating the current state of service for alcoholism and referring to advanced treatment system in other countries. The problems of current state are lack of public awareness for seriousness of alcoholism, difficult access to treatment system and weak capacities to managing alcoholic patients in the community. Integrative and comprehensive intervening system for alcoholism are accomplished by developing system of matching services to alcoholic subjects in the community level based on seriousness and needs of alcoholic subjects. Referring to current state of alcoholism service in Seoul, treatment system should be developed based on continuum of care system in which the different intensity of service is placed and organized in the community level. First, the rehabilitation centers for chronic alcoholics and intensive outpatients centers should be newly established. Second, it is needed to strengthen the function of alcohol counseling centers and community mental health centers as coordination centers for alcoholics. Third, legal and political support should be established to induce improvement of inpatient and outpatient treatment service quality. Finally, setting up a committee to support and organize these integrative treatment systems is needed.