조릿대 추출물의 HL60 세포와 L1210 세포에 대한 세포독성 및 활성산소 소거효소

박시원 ( Sie Won Park ),( Jee Hee Kim ) 상명대학교 자연과학연구소 2003 自然科學硏究 Vol.11 No.-

Farnesol의 ICR 생쥐 Macrophages의 증식에 미치는 영향

박시원 ( Sie Won Park ) 상명대학교 자연과학연구소 2004 自然科學硏究 Vol.13 No.-

Monoterpenes의 세포독성 및 면역세포증식

박시원 ( Sie Won Park ) 상명대학교 자연과학연구소 2005 自然科學硏究 Vol.15 No.-

Monoterepenes, components of essential oils from various flavorable plants were investigated for their anticancer action including cytotoxicities against L1210 cancer cell and immune cell proliferation including macrophages. Both types of compounds such as chain and cyclic monoterpenes showed significant cytotoxicities (linalool, 88.1%) and macrophages proliferation (linaool, 35.7%). In an attempt to get into the reaction mechanism of monoterpenes, generation of O2- ion and the reactive oxygen species (ROS) metabolizing enzyme activities were measured according to the various reaction condition. Elevated amount of O2- ion and concomitantly enhanced activities of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx) suggested that the anticancer reaction of monoterpenes in cell level may be resulted from the increased toxicity of reactive oxygen species(ROS) evoked by monoterpenes, which may kill cancer cell himself in spite of protective effort of concurrently elevated antioxidant enzymes to overcome the toxicity of ROS.

Farnesol이 ICR 마우스의 대식세포의 증식, O2-, ROS 효소 (SOD, GPx), RNS (iNOS) 효소활성에 미치는 영향

박시원 ( Sie Won Park ) 상명대학교 자연과학연구소 2005 自然科學硏究 Vol.15 No.-

The current study was performed to find out the immunestimuating anticancer activity of farnesol, one of the monoterpenes. Farnesol showed generally the proliferating effect on the cell number of macrophages from ICR mouse. The most prominent proliferating effect was found to be 135.7% of control in 1.0 μg/ml for 48 hr. Such effect was accompanied by the coincident alterations of RNS (reactive nitrogen species) related enzymes activities such as inducible nitric oxide synthase (iNOS) and O2- ion together with and ROS (reactive oxygen species) related enzymes activities such as superoxide dismutase(SOD) and glutathione peroxidase (GPx). When L1210 cells was cultured in the presence of farnesol (1.0 μg/ml for 48 hr), O2- ion, SOD and GPx activities were increased considerably with values of 178.4 %, 275,4 %, 175.3% respectively. In addition, farnesol exhibited cytotoxic effect (76.4%) on L1210 cell in 1.0 μg/ml for 48 hr.

황해쑥의 피부암 세포에 대한 사멸작용과 O2(-) 이온의 변화

박시원 ( Sie Won Park ) 상명대학교 자연과학연구소 2003 自然科學硏究 Vol.11 No.-

기아를 일으킨 흰쥐의 생명연장에 미치는 천연약물의 효과

박시원(Sie Won Park),이현아(Hyun Ah Lee) 대한약학회 1995 약학회지 Vol.39 No.1

The current study was undertaken to select the crude drug showing life prolonging effect on the starved rats(Sprague-Dawley) and elucidate the reaction mechanism there of. As distilled water extracts prepared from twenty kinds of natural drugs were administered orally to female starved rats, Bupleuri Radix demonstrated the most prominent effect with 34.6% increment in the survival time. To investigate underlying mechanism of life prolonging effect of Bupleuri Radix, the concentrations of adrenocorticosteroids(corticosterone, testosterone and aldosterone) and enzyme activities of superoxide dismutase(SOD), glutamate oxaloacetate transaminase(GOT), glutamate pyrurate transaminase(GPT) were assayed in the serums of rats starved for 2, 4 and 6 days respectively. The results manifested much elevated values of corticosterone, aldosterone(2 days) and rather decreasing tendency afterwards, specially in the late periods of starvation(6 days). With respect to such a considerable changes according to starvation periods, Bupleur Radix restored these values much near to normal suggesting that Buplerum Radix may play a life prolonging action during starvation probably through reinforcing the homeostatic properties of corticosteroids and some enzyme activities.

감나무의 배 배양법에 의한 기내 증식

박시원(Sie Won Park) 한국생물공학회 1995 KSBB Journal Vol.10 No.1

노화과정에 따른 뇌신경의 핵단백질의 변화

박시원 ( Sie Won Park ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1

Rat cerebellum nuclei showed some significant changes of non-histone chromosomal protein (NHCP), a kind of chromosomal protein, as a function of age by electrophoresis profile analysis. Non-histone chromosomal protein was found to be prominent in peak numbers 23, 24 and 37 during developmental stages generally. In adult cerebellum, peak numbers 1, 4, 22 and 26 were distinct as compared with those of neonatal or fetal rats. Further, electrophoresis profile of high mobility group (HMG) protein included in the nonhistone chromosomal protein category revealed the presence of HMG bands 1, 2 and 17 in both neonatal and adult stages but HMG 14 band was distinct in adult cerebellum exceptionally. The HMG protein pattern obtained from nerve cell types demonstrated no much difference between the neuron and glia cell rich found fractions of adult cerebellum.

덱사메사돈이 흰쥐의 뇌의 푸로스타글란딘 $D_2$ 함량에 미치는 영향

박시원,이상섭,Park, Sie-Won,Lee, Sang-Sup 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.3

본 연구는 덱사메사돈이 흰쥐의 뇌의 $PGD_2$(prostaglandin $D_2$)의 기본 준위에 미치는 영향을 관찰하고자 행하였다. 우선 몇가지 스테로이드 호르몬을 식수에 분산 용해 (10 mg/l)시켜 일주일간 경구투여했을 때 전반적으로 전뇌의 $PGD_2$의 함량이 감소되었으며 그 억제 효과는 덱사메사돈의 경우 40%로 가장 현저하였다. 덱사메사돈을 경구투여하여 얻은 시간의존표에 의하면 투여 첫날부터 제 칠일까지 계속 $PGD_2$의 함량 감소 추세가 증가하였다. 덱사메사돈을 복강내 주사로 투여했을 때도 $PGD_2$의 함량 억제가 신속히 일어났으며 투여후 8시간째에 35%의 최고 억제율을 보였다. 아울러 $PGD_2$의 함량 자체가 오후 5시에 최고치(2.4 ng)를 나타내는 일일주기성을 보였다. 용량의존성을 관찰하기 위해서 체중 100 g당 $10\;{\mu}g$에서 $1,000\;{\mu}g$까지 복강내 주사를 행하였으며, $200\;{\mu}g$ 농도까지는 억제효과의 상관성이 나타났다. 이상과 같은 덱사메사돈의 $PGD_2$ 함량에 대한 억제효과가 뇌의 어느 부위에서 가장 현저한지 밝히기 위하여 뇌를 13개 부분으로 나누어 측청한 바 모든 부분에 있어서 비교적 균등하게(18~29%) $PGD_2$의 함량이 감소되었다. 이들 결과로부터 덱사메사돈은 부신-시상하부로 연결되는 부신피질호르몬 분비에 대한 피드백조절자로서의 작용 이외, 전뇌에 고르게 분포되어 그 부분의 세포막에서 유래되는 $PGD_2$의 함량을 감소시킴으로써 중추신경계의 기능에 영향을 미치리라 유추된다. The present study was performed to investigate the effect of dexamethasone on the basal level of $PGD_2$ of rat brain. Primarily, several steroid hormones were administered orally for one week (10 mg/l drinking water) and dexamethasone thereof was shown to have the most strongly decreasing effect (40%) on the level of $PGD_2$ of the whole brain. When dexamethasone was administered orally to find out time dependency, it was demonstrated that there was a steadily increasing abatement of $PGD_2$ in the whole brain from 1st day onward to 7th day of uptake. In a similar way, there was also a fast reduction of $PGD_2$ content with maximum value of 35% by route of intraperitoneal injection. Furthermore, this result of $PGD_2$ decrease by injection showed typical circadian rhythm with peak value (2.4 ng of $PGD_2$/whole brain) at 5:00 PM. In an attempt to observe the dose dependency, this steroid was administered intraperitoneally in concentrations of $10\;{\mu}g$ to $1,000\;{\mu}g$/100g body weight. According to the injected dose, there was a gradual increment of decreasing effect of dexamethasone rather dose dependently to the concentration of $500\;{\mu}g$/100g body weight. As the effect of dexamethasone on the level of $PGD_2$ was very evident, we dissected the brain into 13 parts in order to find out the responsible area for specially reduced content of $PGD_2$ in the brain. However, the obtained data showed that dexamethasone exerts the decreasing effect on the basal level of $PGD_2$ generally with similar ratio (18~29%) in almost all areas. This result implied that dexamethasone may be involved in the physiological homeostasis by feed-back control acting on adrenal-hypothalamus axis as known already and additionally may take part in some other central nervous functions via $PGD_2$ derived from cell membrane by being distributed overally.

3β - Hydorxy - 5α - halo - 6 , 19 - oxidoandrostan - 17 - one 류의 3β - Hydroxysteroid Dehydrogenase 에 대한 기질의 특이성

박시원,이상섭 ( Sie Won Park,Sang Sup Lee ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.2

Korean BiWhen 3β-acetoxy-5α-chloro-6, 19-oxidoandrostan-l7-one was exposed to the culture broth of Nocardia sp. (ATCC 19170), the compound was hydrolyzed and then dehydrogenated to a 3-keto compound, 6, 19-oxidoandrost-4-ene-3, 17dione. On the other hand, 3 β-acetoxy-5α-bromo-6, 19-oxidoandrostan-l7-one was also hydrolyzed but not further easily oxidized to the corresponding 3-keto compound by the same organism. The discrepancy of this result is considered to be the effect of steroidal 5α-halogen function carrying 6,19-oxido ring on 3β-hydroxysteroid dehydrogenase. In another incubation test, 3 β, 5α-dihydroxy-6β-methylandrostan-l7-one was oxidized to a 3-keto compound much slower rate than dehydroisoandrosterone. In order to clarify the substrate specificity of 5α-halo-6, 19-oxidoandrostane derivatives and 3β, 5α-dihydroxy-6β-methylandrestan-l7-one on 3β-hydroxysteroid dehydrogenase, a cell free enzyme extract was prepared from Rr opaca. This enzyme was induced well with testosterone. It possessed an alkaline optimum pH and required NAD as a cofactor. When reacted with this enzyme, 3β, 5α-dihydroxy-6β-methylandrostan-l7-one and 3β-hydroxy-5 α-chloro-6, 19-oxidoandrostan-l7-one showed relatively retarded turn-over rates to 3-keto compounds as compared with dehydroisoandrosterone. Above all, 3 β-hydroxy-5 α-bromo-6, 19-oxidoandrostan-l7-one showed extremly slow reaction rate to 3-lceto compound. These findings clearly suggest that 5 α-halogen residue of 6,19-oxidosteroids and 5 α-hydroxy residue of 6β-methylsteroid interfere with 3β-hydroxysteroid dehydrogenase activity.